##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936367.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	198558
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.115558174437695	33.0	31.0	34.0	30.0	34.0
2	32.250979562646684	33.0	31.0	34.0	30.0	34.0
3	32.10795837991922	33.0	31.0	34.0	30.0	34.0
4	35.85107122352159	37.0	35.0	37.0	35.0	37.0
5	35.92327682591485	37.0	35.0	37.0	35.0	37.0
6	35.91033350456794	37.0	35.0	37.0	35.0	37.0
7	26.795037218344262	36.0	10.0	37.0	0.0	37.0
8	31.18660038880327	37.0	19.0	37.0	17.0	37.0
9	36.065940430503936	39.0	32.0	39.0	32.0	39.0
10	36.83666737175032	38.0	37.0	39.0	34.0	39.0
11	37.24154655063004	39.0	37.0	39.0	34.0	39.0
12	37.25696773738656	39.0	37.0	39.0	34.0	39.0
13	37.21751830699342	39.0	37.0	39.0	34.0	39.0
14	38.366200304193235	40.0	38.0	41.0	34.0	41.0
15	38.44173994500348	40.0	38.0	41.0	34.0	41.0
16	38.385902355986666	40.0	38.0	41.0	34.0	41.0
17	38.30009871171144	40.0	37.0	41.0	34.0	41.0
18	38.194975775340204	40.0	37.0	41.0	33.0	41.0
19	38.066368517007625	40.0	37.0	41.0	34.0	41.0
20	38.117562626537335	40.0	37.0	41.0	34.0	41.0
21	38.03875441936361	40.0	37.0	41.0	33.0	41.0
22	38.05786722267549	40.0	37.0	41.0	34.0	41.0
23	38.00014101673063	40.0	37.0	41.0	34.0	41.0
24	37.95213992888728	40.0	36.0	41.0	33.0	41.0
25	37.844161403720825	40.0	36.0	41.0	33.0	41.0
26	37.70179494152842	40.0	36.0	41.0	33.0	41.0
27	37.588644124135016	40.0	36.0	41.0	33.0	41.0
28	37.546404577000175	40.0	36.0	41.0	33.0	41.0
29	37.51452975956647	40.0	36.0	41.0	33.0	41.0
30	37.40248189445905	40.0	36.0	41.0	32.0	41.0
31	37.253412101250014	40.0	35.0	41.0	32.0	41.0
32	37.083552412896985	39.0	35.0	41.0	31.0	41.0
33	36.961663594516466	39.0	35.0	41.0	31.0	41.0
34	36.755567642703895	39.0	35.0	41.0	31.0	41.0
35	36.64897410328468	39.0	35.0	41.0	30.0	41.0
36	36.4970890117749	39.0	35.0	41.0	30.0	41.0
37	36.349792000322324	39.0	35.0	41.0	30.0	41.0
38	36.144078808207176	39.0	35.0	41.0	29.0	41.0
39	36.066912438682905	39.0	35.0	41.0	29.0	41.0
40	35.77563734525932	39.0	35.0	40.0	27.0	41.0
41	35.6979421629952	39.0	35.0	40.0	27.0	41.0
42	35.56454537213308	38.0	35.0	40.0	26.0	41.0
43	35.46187511961241	38.0	35.0	40.0	26.0	41.0
44	35.2232597024547	38.0	34.0	40.0	25.0	41.0
45	35.09351927396529	38.0	34.0	40.0	24.0	41.0
46	34.985490385680755	38.0	34.0	40.0	24.0	41.0
47	34.791355674412515	38.0	34.0	40.0	23.0	41.0
48	34.7320480665599	38.0	34.0	40.0	23.0	41.0
49	34.58653390948741	37.0	34.0	40.0	23.0	41.0
50	34.33621410368759	37.0	33.0	40.0	23.0	41.0
51	32.216858550146554	35.0	30.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	12.0
10	13.0
11	9.0
12	7.0
13	5.0
14	6.0
15	6.0
16	26.0
17	46.0
18	111.0
19	128.0
20	262.0
21	394.0
22	646.0
23	888.0
24	1363.0
25	1841.0
26	2293.0
27	2677.0
28	3031.0
29	3446.0
30	3995.0
31	5050.0
32	6268.0
33	8584.0
34	12999.0
35	16152.0
36	18448.0
37	29582.0
38	37919.0
39	42337.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.529215644799	21.550378227016793	25.92995497537244	11.990451152811774
2	30.393134499743148	25.92995497537244	27.181982090875213	16.494928434009207
3	31.723728079452858	24.958954058763684	27.56776357537848	15.74955428640498
4	27.767705154161504	26.138458284229294	28.157515688111285	17.93632087349792
5	25.825703320944005	30.764814311183635	25.740086020205684	17.669396347666677
6	26.13291834124034	35.31713655455837	24.391865349167496	14.158079755033794
7	62.830507962408966	27.86591323442017	6.111564379173844	3.1920144239970187
8	86.33648606452523	4.780467168283323	6.054150424561086	2.828896342630365
9	79.35716516080944	7.779590850028708	7.894922390435037	4.96832159872682
10	38.627504306046596	34.683064897913965	13.615668973297476	13.07376182274197
11	33.32074255381299	25.24249841356178	23.25063709344373	18.186121939181497
12	30.300466362473433	22.769669315766677	26.591222715780777	20.33864160597911
13	26.656694769286553	23.894277742523595	29.40198833590185	20.047039152287997
14	21.49951147775461	26.342428912458825	30.076854118192166	22.081205491594396
15	21.12833529749494	24.14911512001531	33.271890329274065	21.450659253215683
16	26.245731725742605	25.23292942112632	28.965843733317215	19.555495119813855
17	26.225082847329244	23.781967989202148	27.65841718792494	22.33453197554367
18	26.86217629105853	23.20027397536236	28.90842977870446	21.029119954874645
19	26.31019651688675	25.89470079271548	26.666263761722014	21.12883892867575
20	28.285438008037957	26.363581422053002	26.146012751941498	19.204967817967546
21	27.52545855619013	25.697781001017333	27.127589923347333	19.6491705194452
22	26.473876650651196	23.30956194159893	27.302349943089677	22.9142114646602
23	24.591806927950525	26.334874444746625	28.05427129604448	21.019047331258374
24	24.66281892444525	24.54748738403892	27.98023751246487	22.809456179050958
25	24.88492027518408	26.471358494747125	26.41797358958088	22.225747640487917
26	23.8353528943684	25.803543548988205	26.616404274821466	23.744699281821934
27	23.254666142890237	25.875059176663743	27.945990592169544	22.924284088276472
28	21.174669366129795	27.052045246225287	28.18168998479034	23.59159540285458
29	23.676205441231275	26.833972944932967	27.819075534604497	21.670746079231257
30	24.688000483485933	25.106014363561275	28.63797983460752	21.568005318345268
31	25.817645222050988	26.05938818884155	26.57611378035637	21.546852808751098
32	25.913838777586395	26.323290927587912	27.172916729620567	20.58995356520513
33	26.091116953232806	25.67360670433828	26.133421972421157	22.101854370007757
34	23.540225022411587	26.889876006003284	28.27385449087924	21.296044480705888
35	23.26322787296407	27.146727908218253	27.584887035526144	22.005157183291534
36	24.499642421861623	28.760362211545242	25.95916558385963	20.78082978273351
37	23.942626335881705	29.105853201583415	25.479708699725016	21.47181176280986
38	24.116882724443236	28.773456622246396	25.210266017989706	21.89939463532066
39	23.69987610672952	26.72569224105803	25.37696793883903	24.19746371337342
40	25.55072069621974	24.648213620201652	27.183996615598467	22.617069067980136
41	22.758085798607965	26.037228416885743	26.437615205632607	24.76707057887368
42	24.627564741788294	26.03471026098168	26.29206579437746	23.045659202852566
43	24.22063074769085	25.656483244190614	26.214002961351348	23.90888304676719
44	23.69584705728301	25.301423261716984	27.00722207113287	23.995507609867143
45	23.548283121304607	25.178537253598442	26.652665719840048	24.620513905256903
46	23.59663171466272	26.348472486628594	26.13644375950604	23.918452039202652
47	22.682541121485915	26.741808438844068	27.773245097150458	22.802405342519567
48	23.427915269090143	26.340918018916387	27.333071445119312	22.898095266874165
49	23.744195650641124	25.12716687315545	28.287956163942024	22.840681312261406
50	22.19603340081991	26.577624673898807	27.533013023902335	23.693328901378944
51	22.666928554880688	25.14630485802637	26.59676265876973	25.590003928323213
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	62.0
1	210.0
2	358.0
3	977.5
4	1597.0
5	1089.5
6	582.0
7	526.0
8	470.0
9	461.5
10	453.0
11	451.0
12	449.0
13	439.0
14	429.0
15	415.5
16	402.0
17	438.0
18	474.0
19	480.0
20	486.0
21	552.0
22	618.0
23	708.0
24	798.0
25	983.5
26	1502.5
27	1836.0
28	2147.0
29	2458.0
30	2932.5
31	3407.0
32	3804.0
33	4201.0
34	4673.5
35	5146.0
36	5750.0
37	6354.0
38	6909.5
39	7465.0
40	8237.0
41	9009.0
42	9688.0
43	10367.0
44	11305.0
45	12243.0
46	13366.0
47	14489.0
48	14861.5
49	15234.0
50	15233.0
51	15232.0
52	14569.0
53	13906.0
54	13079.5
55	12253.0
56	11861.0
57	11469.0
58	11319.0
59	11169.0
60	10361.0
61	9553.0
62	8558.0
63	7563.0
64	6415.0
65	5267.0
66	4598.5
67	3930.0
68	3322.5
69	2715.0
70	2243.0
71	1771.0
72	1520.0
73	1269.0
74	1083.5
75	681.0
76	464.0
77	358.0
78	252.0
79	198.5
80	145.0
81	105.0
82	65.0
83	51.0
84	37.0
85	26.0
86	15.0
87	13.5
88	12.0
89	11.0
90	10.0
91	7.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	198558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.84323975865994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.85326466761997	31.784667452331313
2	10.90516175506123	10.21666213398604
3	5.948758748965177	8.359773970326051
4	4.098439969465977	7.6793682450467875
5	2.9845932201567558	6.990400789693692
6	2.1126533420778184	5.937811621793129
7	1.5546548257732957	5.097754812195933
8	1.1912569481029125	4.46418678673234
9	0.900968702626571	3.798386365696673
>10	2.4373461203513562	14.55191933843008
>50	0.006450849899474255	0.19440163579407527
>100	0.00537570824956188	0.5313308957584182
>500	0.001075141649912376	0.3933359522154735
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	781	0.3933359522154735	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG	315	0.15864382195630494	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC	287	0.1445421488935223	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06043574169763998	0.0
2	0.0	0.0	0.0	0.2649100011079886	0.0
3	0.0	0.0	0.0	0.34246920295329325	0.0
4	0.0	0.0	0.0	0.45226080037067257	0.0
5	0.0	0.0	0.0	0.8758146234349661	0.0
6	0.0	0.0	0.0	1.0853251946534515	0.0
7	0.0	0.0	0.0	1.3451988839533033	0.0
8	0.0	0.0	0.0	1.8926459774977589	0.0
9	0.0	0.0	0.0	2.1515124044359832	0.0
10	0.0	0.0	0.0	2.9155209057303155	0.0
11	0.0	0.0	0.0	3.4997330754741687	0.0
12	0.0	0.0	0.0	4.226976500569103	0.0
13	0.0	0.0	0.0	4.460661368466645	0.0
14	0.0	0.0	0.0	4.571963859426464	0.0
15	0.0	0.0	0.0	4.764854601678099	0.0
16	0.0	0.0	0.0	5.039333595221548	0.0
17	0.0	0.0	0.0	5.364175706846362	0.0
18	0.0	0.0	0.0	5.746431773083935	0.0
19	0.0	0.0	0.0	5.959467762568116	0.0
20	0.0	0.0	0.0	6.189627212199961	0.0
21	0.0	0.0	0.0	6.4782078788061925	0.0
22	0.0	0.0	0.0	6.801035465707753	0.0
23	0.0	0.0	0.0	7.104725067738394	0.0
24	0.0	0.0	0.0	7.368124175303941	0.0
25	0.0	0.0	0.0	7.588211001319514	0.0
26	5.036311808136665E-4	0.0	0.0	7.791174367187422	0.0
27	5.036311808136665E-4	0.0	0.0	8.041982695232628	0.0
28	5.036311808136665E-4	0.0	0.0	8.273653038406914	0.0
29	5.036311808136665E-4	0.0	0.0	8.52597225999456	0.0
30	5.036311808136665E-4	0.0	0.0	8.846281690992052	0.0
31	5.036311808136665E-4	0.0	0.0	9.095579125494817	0.0
32	5.036311808136665E-4	0.0	0.0	9.377612586750471	0.0
33	5.036311808136665E-4	0.0	0.0	9.626910021253236	0.0
34	5.036311808136665E-4	0.0	0.0	9.888798235276342	0.0
35	5.036311808136665E-4	0.0	0.0	10.231267438229636	0.0
36	5.036311808136665E-4	0.0	0.0	10.532438884356209	0.0
37	5.036311808136665E-4	0.0	0.0	10.836632117567662	0.0
38	5.036311808136665E-4	0.0	0.0	11.152912499118646	0.0
39	5.036311808136665E-4	0.0	0.0	11.65352189284743	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGT	20	7.0191786E-4	45.000004	45
GCGATGC	20	7.0191786E-4	45.000004	9
CGGTCTA	20	7.0191786E-4	45.000004	31
GTAACGG	20	7.0191786E-4	45.000004	2
ATTACGG	30	2.1564065E-6	44.999996	2
CGGGATT	60	3.6379788E-12	41.249996	6
CCGCACT	40	3.4389814E-7	39.375004	34
TCCGCCC	35	6.222219E-6	38.571426	35
GGATCGT	35	6.222219E-6	38.571426	8
CGCGCAA	35	6.222219E-6	38.571426	18
AACGACG	35	6.222219E-6	38.571426	13
CGCAATC	35	6.222219E-6	38.571426	20
ACGCGCA	35	6.222219E-6	38.571426	17
CGTTTTT	320	0.0	37.968754	1
TTAGGAT	30	1.1365239E-4	37.499996	5
GATCGTT	30	1.1365239E-4	37.499996	9
TCACACG	30	1.1365239E-4	37.499996	38
AGGCGAT	60	1.546141E-10	37.499996	7
ATCGTTT	30	1.1365239E-4	37.499996	10
TGTTGCG	30	1.1365239E-4	37.499996	2
>>END_MODULE