##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936366.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	287973
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02590173384311	33.0	31.0	34.0	30.0	34.0
2	32.15994902299868	33.0	31.0	34.0	30.0	34.0
3	31.762866657638043	33.0	31.0	34.0	30.0	34.0
4	35.63981692728138	37.0	35.0	37.0	33.0	37.0
5	35.823115361509586	37.0	35.0	37.0	35.0	37.0
6	35.843502689488254	37.0	35.0	37.0	35.0	37.0
7	26.88078048983759	36.0	10.0	37.0	0.0	37.0
8	31.252718136769765	37.0	19.0	37.0	17.0	37.0
9	36.11256958117601	39.0	32.0	39.0	32.0	39.0
10	36.81028429748622	38.0	35.0	39.0	34.0	39.0
11	37.278036482586906	39.0	37.0	39.0	34.0	39.0
12	37.337785139579054	39.0	37.0	39.0	35.0	39.0
13	37.327933521545425	39.0	37.0	39.0	34.0	39.0
14	38.53159844846566	40.0	38.0	41.0	34.0	41.0
15	38.60378577158275	40.0	38.0	41.0	34.0	41.0
16	38.55133988255844	40.0	38.0	41.0	34.0	41.0
17	38.475402207845875	40.0	38.0	41.0	34.0	41.0
18	38.33565299524608	40.0	38.0	41.0	34.0	41.0
19	38.175565070336454	40.0	37.0	41.0	34.0	41.0
20	38.160570609050154	40.0	37.0	41.0	34.0	41.0
21	38.10921510002674	40.0	37.0	41.0	34.0	41.0
22	38.1016761988103	40.0	37.0	41.0	34.0	41.0
23	38.00741041694881	40.0	36.0	41.0	34.0	41.0
24	37.970952137874036	40.0	36.0	41.0	34.0	41.0
25	37.858205456761574	40.0	36.0	41.0	33.0	41.0
26	37.78593826504568	40.0	36.0	41.0	33.0	41.0
27	37.668020960298364	40.0	36.0	41.0	33.0	41.0
28	37.60523035145656	40.0	36.0	41.0	33.0	41.0
29	37.59546207456949	40.0	36.0	41.0	33.0	41.0
30	37.515225385713244	40.0	36.0	41.0	33.0	41.0
31	37.34099030117407	40.0	35.0	41.0	33.0	41.0
32	37.14145076100885	40.0	35.0	41.0	32.0	41.0
33	36.952978230598006	39.0	35.0	41.0	31.0	41.0
34	36.67988665604067	39.0	35.0	41.0	30.0	41.0
35	36.45865758248169	39.0	35.0	41.0	30.0	41.0
36	36.248978202817625	39.0	35.0	41.0	29.0	41.0
37	36.125883329339906	39.0	35.0	41.0	29.0	41.0
38	35.97117090838377	39.0	35.0	41.0	27.0	41.0
39	35.93737260090356	39.0	35.0	41.0	27.0	41.0
40	35.76376257496363	39.0	35.0	41.0	26.0	41.0
41	35.6466995169686	39.0	35.0	41.0	26.0	41.0
42	35.558962124921436	39.0	35.0	40.0	25.0	41.0
43	35.49895649939404	39.0	35.0	40.0	25.0	41.0
44	35.304920947449936	39.0	35.0	40.0	24.0	41.0
45	35.24709608192435	38.0	34.0	40.0	24.0	41.0
46	35.14007910463828	38.0	34.0	40.0	23.0	41.0
47	35.02689488250635	38.0	34.0	40.0	23.0	41.0
48	34.93280967312908	38.0	34.0	40.0	23.0	41.0
49	34.782746993641766	38.0	34.0	40.0	23.0	41.0
50	34.59363898698837	38.0	34.0	40.0	23.0	41.0
51	32.683609227253946	36.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	20.0
10	18.0
11	17.0
12	3.0
13	10.0
14	8.0
15	19.0
16	25.0
17	41.0
18	102.0
19	209.0
20	348.0
21	515.0
22	879.0
23	1375.0
24	2061.0
25	3030.0
26	4097.0
27	4784.0
28	4860.0
29	5027.0
30	5627.0
31	6636.0
32	8297.0
33	11196.0
34	16728.0
35	21550.0
36	25735.0
37	42619.0
38	56485.0
39	65634.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.25383976970063	21.277341973032193	25.901386588325988	15.567431668941186
2	34.140006181135035	23.67339993679963	26.27468547398541	15.911908408079926
3	30.776149152871973	23.144878165661364	30.729269757928694	15.34970292353797
4	26.50352637226407	25.444052046546027	30.29277050279019	17.75965107839971
5	24.29533324304709	30.015661190458825	28.784643004726135	16.904362561767943
6	26.083348091661374	33.80664159487174	26.549711257652625	13.560299055814259
7	60.97307733711147	27.43555819469186	8.442458147117959	3.1489063210787123
8	84.23984192962534	3.892031544623975	9.334208415372276	2.5339181103784036
9	76.7783785285426	8.274734089654238	11.083677983699861	3.8632093981032942
10	31.706444701412977	39.606143631521014	16.879707472575554	11.807704194490455
11	30.363263222593783	25.214863893490012	27.610227347702736	16.811645536213465
12	28.84020376910335	22.75768908890764	29.710771495938854	18.69133564605015
13	25.403075982817835	22.963958426658053	32.02834988002348	19.604615710500635
14	21.068989106617632	25.836449944960115	32.22697961267202	20.867581335750227
15	20.290791150559254	25.62636080465877	34.388987856500435	19.69386018828154
16	24.445347306865575	25.201320957173067	32.0339059564612	18.31942577950016
17	23.85987575224066	24.558205109506794	30.920954395030087	20.660964743222454
18	24.336656561552644	24.543967663635133	31.7324888097148	19.38688696509742
19	23.699791299878807	25.614206887451253	30.39486340733333	20.29113840533661
20	24.831494619287227	26.179190410212065	30.029898636330486	18.959416334170218
21	24.69050917968004	25.375990110183942	31.13937764998802	18.794123060148
22	24.45889024318252	23.328575942883532	31.604351796869846	20.6081820170641
23	21.78120865497807	25.45828949241769	32.05856104565358	20.701940806950653
24	21.915596253815462	24.907196160751184	32.103704166710074	21.073503418723284
25	21.819059425710048	26.67229219405986	30.436186725838883	21.072461654391212
26	21.668003597559494	26.979959926798692	30.101433120466154	21.25060335517566
27	21.722522597604637	25.47738850517236	32.071409472415816	20.728679424807186
28	20.669993367433754	26.415670913592592	31.977650682529262	20.936685036444388
29	21.945807419445572	25.69025568369257	31.331409541866773	21.032527354995086
30	22.791720057088686	25.229448594139033	31.190771357036944	20.788059991735334
31	23.438655707305895	25.945835199827762	30.055942744632308	20.559566348234036
32	23.8866143700972	26.300035072732513	30.188594069582912	19.624756487587376
33	23.896684758640564	26.372958575977606	29.339903393720938	20.390453271660885
34	22.307994152229547	25.969101269910723	31.722418421171426	20.000486156688304
35	22.563573668364743	26.815361162331193	30.08233410771149	20.53873106159258
36	23.344549662641985	27.64252204199699	29.098908578234767	19.91401971712626
37	22.490997419897006	28.817632208575112	29.029457622763243	19.66191274876464
38	21.948238202887076	28.13770735450893	28.924933934778608	20.989120507825387
39	23.061189764318183	27.02927010518347	28.56969229754178	21.33984783295656
40	23.658120726595897	26.636524951992023	29.326360457403993	20.378993864008084
41	21.27248040614919	27.37652488254107	29.900025349598746	21.450969361710996
42	22.418768426206622	27.522024634253906	28.75859889642432	21.300608043115155
43	21.724258871491426	27.551888545106664	29.693408757070976	21.03044382633094
44	22.021161706132172	27.12615418806624	29.478110795109263	21.37457331069232
45	22.203470464244912	26.625065544339225	28.521771138266434	22.64969285314943
46	21.805516489393103	27.1060134109795	29.02911036798589	22.05935973164151
47	21.54958971848055	27.14282241737941	30.145534477190573	21.16205338694947
48	21.69300594152924	26.81605567188591	30.027815107666346	21.46312327891851
49	22.199997916471336	26.35837387532859	30.10421115868502	21.337417049515057
50	21.124897125772208	26.43650620023405	30.204567789341365	22.23402888465238
51	21.31831803676039	25.98993655655218	29.636806228361685	23.054939178325746
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	284.5
2	438.0
3	3365.5
4	6293.0
5	4236.0
6	2179.0
7	1836.5
8	1494.0
9	1455.5
10	1417.0
11	1371.0
12	1325.0
13	1252.0
14	1179.0
15	1117.0
16	1055.0
17	1021.5
18	988.0
19	1001.5
20	1015.0
21	1113.5
22	1212.0
23	1426.5
24	1641.0
25	1938.5
26	2608.0
27	2980.0
28	3785.0
29	4590.0
30	5112.5
31	5635.0
32	6614.5
33	7594.0
34	8676.5
35	9759.0
36	10403.0
37	11047.0
38	11698.5
39	12350.0
40	13446.5
41	14543.0
42	15434.5
43	16326.0
44	17482.0
45	18638.0
46	20143.0
47	21648.0
48	22144.0
49	22640.0
50	22281.0
51	21922.0
52	20269.0
53	18616.0
54	17433.0
55	16250.0
56	15325.0
57	14400.0
58	13434.0
59	12468.0
60	11477.5
61	10487.0
62	9198.5
63	7910.0
64	6718.5
65	5527.0
66	4626.0
67	3725.0
68	3078.5
69	2432.0
70	1933.5
71	1435.0
72	1212.5
73	990.0
74	796.5
75	483.5
76	364.0
77	290.0
78	216.0
79	166.5
80	117.0
81	87.0
82	57.0
83	49.5
84	42.0
85	35.5
86	29.0
87	22.0
88	15.0
89	10.0
90	5.0
91	5.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	287973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.18184910364663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.22050858512839	33.55210759007814
2	11.53076380600699	11.342085711136518
3	6.445507182666743	9.510058849631589
4	4.09415024093201	8.054315174287066
5	2.8518897302841286	7.013060518753676
6	1.9132722319372422	5.645895972320079
7	1.412089045755099	4.861440525846775
8	1.020348047107042	4.014608294881525
9	0.7072075392293214	3.1303597031403974
>10	1.7901793720852894	10.999576065412796
>50	0.008450531320682735	0.2763824829125396
>100	0.004225265660341367	0.2950850569442152
>500	7.042109433902281E-4	0.19395261958774707
>1k	7.042109433902281E-4	1.111071435066951
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3208	1.113993325763179	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	560	0.19446267532025574	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06146409559229511	0.0
2	0.0	0.0	0.0	0.22918815305601567	0.0
3	0.0	0.0	0.0	0.3403096818104475	0.0
4	0.0	0.0	0.0	0.4403190576894362	0.0
5	0.0	0.0	0.0	0.734443854111323	0.0
6	0.0	0.0	0.0	1.0587798161633208	0.0
7	0.0	0.0	0.0	1.3501265743663469	0.0
8	0.0	0.0	0.0	1.9685873328402315	0.0
9	0.0	0.0	0.0	2.354040135707167	0.0
10	0.0	0.0	0.0	2.9461095311018743	0.0
11	0.0	0.0	0.0	3.5215106971834165	0.0
12	0.0	0.0	0.0	4.000375035159546	0.0
13	0.0	0.0	0.0	4.256301806072097	0.0
14	0.0	0.0	0.0	4.404579596003792	0.0
15	3.472547773575995E-4	0.0	0.0	4.564316793588288	0.0
16	3.472547773575995E-4	0.0	0.0	4.8504547301309495	0.0
17	3.472547773575995E-4	0.0	0.0	5.167845596635796	0.0
18	3.472547773575995E-4	0.0	0.0	5.503988221117952	0.0
19	3.472547773575995E-4	0.0	0.0	5.719633437857022	0.0
20	3.472547773575995E-4	0.0	0.0	5.958891979456407	0.0
21	3.472547773575995E-4	0.0	0.0	6.2502387376594335	0.0
22	3.472547773575995E-4	0.0	0.0	6.565546075500134	0.0
23	3.472547773575995E-4	0.0	0.0	6.873561063016324	0.0
24	3.472547773575995E-4	0.0	0.0	7.135043910366597	0.0
25	3.472547773575995E-4	0.0	0.0	7.3545089296566	0.0
26	3.472547773575995E-4	0.0	0.0	7.575015713278676	0.0
27	3.472547773575995E-4	0.0	0.0	7.794827987346036	0.0
28	3.472547773575995E-4	0.0	0.0	8.024363395179408	0.0
29	3.472547773575995E-4	0.0	0.0	8.266399974997656	0.0
30	6.94509554715199E-4	0.0	0.0	8.562608300083689	0.0
31	6.94509554715199E-4	0.0	0.0	8.833814281199974	0.0
32	6.94509554715199E-4	0.0	0.0	9.085573994784234	0.0
33	6.94509554715199E-4	0.0	0.0	9.330388612821341	0.0
34	6.94509554715199E-4	0.0	0.0	9.617568313696076	0.0
35	6.94509554715199E-4	0.0	0.0	9.889468804367077	0.0
36	6.94509554715199E-4	0.0	0.0	10.171786938358805	0.0
37	6.94509554715199E-4	0.0	0.0	10.422504887610991	0.0
38	6.94509554715199E-4	0.0	0.0	10.7336451681234	0.0
39	6.94509554715199E-4	0.0	0.0	11.139933257631792	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGG	20	7.0244714E-4	45.000004	2
GTATGCG	20	7.0244714E-4	45.000004	1
TATCGCG	20	7.0244714E-4	45.000004	1
CCCGTGA	25	3.883501E-5	45.0	37
TAGGCGA	25	3.883501E-5	45.0	6
CACGACG	65	0.0	44.999996	26
CGTTTTT	1150	0.0	43.630436	1
TCTCACG	80	0.0	42.187504	23
TCACGAC	75	0.0	42.000004	25
GCGATAA	40	3.4462028E-7	39.375004	9
CGTTTTA	225	0.0	39.0	1
AGGCGAT	110	0.0	38.863636	7
GCGATTT	35	6.231994E-6	38.571426	9
CGGGATA	65	9.094947E-12	38.07692	6
AGTTGCG	30	1.1377984E-4	37.499996	1
CGGGCTA	30	1.1377984E-4	37.499996	6
CGATGTT	30	1.1377984E-4	37.499996	10
TCGACGG	30	1.1377984E-4	37.499996	2
CTCACGA	85	0.0	37.058823	24
ATAGGGC	80	0.0	36.562504	4
>>END_MODULE