##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936365.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136188
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80381531412459	31.0	31.0	34.0	30.0	34.0
2	31.92648397803037	33.0	31.0	34.0	30.0	34.0
3	31.788366082180517	33.0	31.0	34.0	30.0	34.0
4	35.593789467500805	37.0	35.0	37.0	33.0	37.0
5	35.69285840162129	37.0	35.0	37.0	35.0	37.0
6	35.66209945075924	37.0	35.0	37.0	33.0	37.0
7	27.046230211178663	35.0	18.0	37.0	0.0	37.0
8	31.28445971744941	35.0	25.0	37.0	17.0	37.0
9	35.967486122125294	39.0	32.0	39.0	32.0	39.0
10	36.524708491203334	37.0	35.0	39.0	32.0	39.0
11	36.89113578288836	39.0	37.0	39.0	33.0	39.0
12	37.02020736041354	39.0	37.0	39.0	33.0	39.0
13	37.01710135988486	39.0	37.0	39.0	33.0	39.0
14	38.14488794901166	40.0	37.0	41.0	33.0	41.0
15	38.20997444708785	40.0	37.0	41.0	33.0	41.0
16	38.141774605692135	40.0	37.0	41.0	33.0	41.0
17	38.07709930390343	40.0	37.0	41.0	33.0	41.0
18	37.98402942990572	40.0	37.0	41.0	33.0	41.0
19	37.88620877022939	40.0	37.0	41.0	33.0	41.0
20	37.94374687931389	40.0	37.0	41.0	33.0	41.0
21	37.8942050694628	40.0	37.0	41.0	33.0	41.0
22	37.913810321026816	40.0	37.0	41.0	33.0	41.0
23	37.871332275971454	40.0	36.0	41.0	33.0	41.0
24	37.84783534525803	40.0	36.0	41.0	33.0	41.0
25	37.770537786001704	39.0	36.0	41.0	33.0	41.0
26	37.665337621523186	39.0	36.0	41.0	33.0	41.0
27	37.540833259905426	39.0	36.0	41.0	33.0	41.0
28	37.5260962786736	39.0	36.0	41.0	33.0	41.0
29	37.54338120832966	40.0	36.0	41.0	33.0	41.0
30	37.419199929509205	39.0	36.0	41.0	32.0	41.0
31	37.22790554233853	39.0	36.0	41.0	32.0	41.0
32	37.14868417188005	39.0	36.0	41.0	31.0	41.0
33	37.053191176902516	39.0	35.0	41.0	31.0	41.0
34	36.89914676770347	39.0	35.0	41.0	31.0	41.0
35	36.829639909536816	39.0	35.0	41.0	31.0	41.0
36	36.61586189678973	39.0	35.0	41.0	30.0	41.0
37	36.51518489147355	39.0	35.0	41.0	30.0	41.0
38	36.39630510764531	39.0	35.0	40.0	30.0	41.0
39	36.431895614885306	39.0	35.0	40.0	30.0	41.0
40	36.31703233765089	39.0	35.0	40.0	30.0	41.0
41	36.28237436484859	39.0	35.0	40.0	30.0	41.0
42	36.1561958469175	39.0	35.0	40.0	29.0	41.0
43	36.09543425265075	39.0	35.0	40.0	29.0	41.0
44	35.863989485123504	39.0	35.0	40.0	28.0	41.0
45	35.779936558287076	38.0	35.0	40.0	28.0	41.0
46	35.61075865715041	38.0	35.0	40.0	27.0	41.0
47	35.44147061415103	38.0	34.0	40.0	26.0	41.0
48	35.33330396216994	38.0	34.0	40.0	26.0	41.0
49	35.1444033248157	38.0	34.0	40.0	26.0	41.0
50	34.895262431344904	38.0	34.0	40.0	24.0	41.0
51	32.606632008693865	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	9.0
11	3.0
12	6.0
13	1.0
14	9.0
15	4.0
16	9.0
17	21.0
18	39.0
19	72.0
20	132.0
21	231.0
22	334.0
23	541.0
24	766.0
25	1044.0
26	1337.0
27	1588.0
28	1896.0
29	2198.0
30	2846.0
31	3664.0
32	4529.0
33	6092.0
34	9143.0
35	11702.0
36	13280.0
37	20392.0
38	26426.0
39	27856.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.00417070520164	23.14374247363938	26.447264076130057	11.40482274502893
2	29.792639586454015	24.917026463418214	28.336564161306427	16.953769788821337
3	29.588510000881136	25.139513026110965	29.529033395012778	15.742943577995124
4	27.19329162628132	25.711516433165915	29.38070901988428	17.714482920668488
5	24.477927570711074	30.248626898111436	26.95832231914706	18.31512321203043
6	25.637354245601667	34.65136429053955	26.050753370341003	13.660528093517785
7	62.90715775251857	26.715275942080062	6.661379857256146	3.716186448145211
8	84.7504919669868	4.179516550650572	7.099744470878491	3.9702470114841253
9	78.12142038946163	7.474226804123711	9.179957118101449	5.224395688313214
10	35.570681704702324	36.293212324140164	15.760566276030193	12.375539695127324
11	25.586688988750844	26.41128440097512	29.07451464152495	18.927511968749084
12	25.918583135077984	23.439656944811585	31.27441477956942	19.36734514054102
13	23.20689047493171	26.83716627015596	31.51011836578847	18.44582488912386
14	18.69841689429318	29.428437160395926	30.429993832055686	21.443152113255206
15	17.12265397832408	27.537668517049962	35.77113989485123	19.568537609774722
16	20.290333950127764	27.06919846095104	31.93232883954533	20.708138749375863
17	19.978999618174875	26.68149910417952	29.95050958968485	23.38899168796076
18	20.77716098334655	26.016242253355653	33.1497635621347	20.056833201163098
19	20.272711252092694	29.076717478779337	29.280847064352216	21.36972420477575
20	21.43140364789849	28.049461039151762	30.93738067964872	19.581754633301024
21	20.67069051605134	27.914353687549564	31.903691955238344	19.511263841160748
22	20.04581901489118	24.997797162745616	29.899844332834025	25.05653948952918
23	17.262167004435046	27.977501688841894	30.81108467706406	23.949246629659
24	19.401121978441566	25.754838899168796	32.44191852439275	22.402120597996884
25	18.199841395717684	29.39319176432578	29.39319176432578	23.013775075630747
26	18.82691573413223	28.403383558022732	30.05404294064088	22.71565776720416
27	18.97744294651511	28.404852116192323	31.024025611654476	21.59367932563809
28	16.80911680911681	27.503157400064616	33.306165007195936	22.38156078362264
29	19.899697477017064	24.649748876553	32.04026786501014	23.4102857814198
30	21.833788586365905	26.87388022439569	30.263312479807325	21.029018709431078
31	20.470966604987222	27.8152260111023	29.048814873557145	22.664992510353336
32	21.8881252386407	28.11701471495286	28.423209093312185	21.571650953094252
33	22.181102593473728	27.254236790319265	28.15593150644697	22.408729109760035
34	19.4774870032602	26.399535935618413	30.343348900049932	23.779628161071457
35	21.143566246659027	26.770346873439653	29.423297206802363	22.662789673098953
36	22.438834552236614	27.09710106617323	29.641378094986344	20.822686286603812
37	18.90621787529004	27.888653919581756	31.951420095749995	21.253708109378213
38	18.323200281963167	29.048080594472346	30.94545774958146	21.683261373983022
39	20.5546744206538	26.876083061650075	30.161247687020882	22.407994830675243
40	21.10905512967369	26.388521749346495	30.007049079214028	22.495374041765796
41	19.14192146150909	27.58392809939202	29.25881869180838	24.01533174729051
42	20.893911357828884	27.368050048462422	29.405674508767294	22.332364084941407
43	20.63691367815079	26.602196963021708	30.73545393133022	22.025435427497282
44	20.12218403970981	25.665991129908655	30.001174846535672	24.21064998384586
45	20.5010720474638	25.571269127970158	29.501865068875375	24.425793755690663
46	22.019561194818927	27.136017857667344	28.66625547037918	22.17816547713455
47	18.862161130202367	26.734367198284726	32.120304285252736	22.283167386260168
48	19.82626956853761	25.43249038094399	31.335359943607365	23.405880106911034
49	20.268305577583927	24.556495432784093	32.058624842129994	23.116574147501982
50	19.230034951684434	23.626163832349402	32.37950480218522	24.76429641378095
51	19.53696360912856	24.17173319235175	29.49085088260346	26.800452315916235
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	87.0
1	144.0
2	201.0
3	523.0
4	845.0
5	608.0
6	371.0
7	320.0
8	269.0
9	279.0
10	289.0
11	298.0
12	307.0
13	304.5
14	302.0
15	317.0
16	332.0
17	350.0
18	368.0
19	424.5
20	481.0
21	547.5
22	614.0
23	671.5
24	729.0
25	905.5
26	1341.0
27	1600.0
28	1947.5
29	2295.0
30	2647.5
31	3000.0
32	3605.5
33	4211.0
34	4762.0
35	5313.0
36	5566.5
37	5820.0
38	6337.5
39	6855.0
40	7498.5
41	8142.0
42	8867.5
43	9593.0
44	10669.0
45	11745.0
46	12998.5
47	14252.0
48	14516.5
49	14781.0
50	13796.5
51	12812.0
52	10773.0
53	8734.0
54	7221.5
55	5709.0
56	4966.5
57	4224.0
58	3798.0
59	3372.0
60	2874.5
61	2377.0
62	2095.0
63	1813.0
64	1577.5
65	1342.0
66	1067.5
67	793.0
68	627.0
69	461.0
70	363.5
71	266.0
72	222.0
73	178.0
74	135.5
75	77.5
76	62.0
77	47.0
78	32.0
79	22.0
80	12.0
81	12.0
82	12.0
83	8.0
84	4.0
85	5.5
86	7.0
87	3.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	136188.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.63421153111875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06477459840009	43.07868534672659
2	9.843249620765212	11.149293623520427
3	4.450984713920834	7.562340294299057
4	2.870515629659402	6.502775574940524
5	1.8488506268718639	5.235409874585132
6	1.2744881951017129	4.330778042118248
7	1.030740707127021	4.086263106881663
8	0.7390216390722038	3.348312626663142
9	0.48619844675802876	2.478191911181602
>10	1.3665417676878995	10.343789467500809
>50	0.012965291913547434	0.47067289335330575
>100	0.01166876272219269	1.4134872382295063
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	488	0.35832819337973976	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGC	263	0.1931153993009663	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	217	0.15933856140041708	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTC	194	0.14245014245014245	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCG	191	0.1402473051957588	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGC	178	0.13070167709342967	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGCT	146	0.10720474638000412	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGC	137	0.10059623461685317	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17108702675712986	0.0
2	0.0	0.0	0.0	0.8245954122242782	0.0
3	0.0	0.0	0.0	1.1542867212970305	0.0
4	0.0	0.0	0.0	1.5625458924427997	0.0
5	0.0	0.0	0.0	2.709489822891885	0.0
6	0.0	0.0	0.0	3.297647369812318	0.0
7	0.0	0.0	0.0	3.9364701735835754	0.0
8	0.0	0.0	0.0	5.358034481745822	0.0
9	0.0	0.0	0.0	5.916820865274474	0.0
10	0.0	0.0	0.0	7.464681176021382	0.0
11	0.0	0.0	0.0	9.268070608276794	0.0
12	0.0	0.0	0.0	10.768937057596851	0.0
13	0.0	0.0	0.0	11.382794372485094	0.0
14	0.0	0.0	0.0	11.60160953975387	0.0
15	0.0	0.0	0.0	12.004728757306077	0.0
16	0.0	0.0	0.0	12.847681146650219	0.0
17	0.0	0.0	0.0	14.054835962052456	0.0
18	0.0	0.0	0.0	15.366992686580316	0.0
19	0.0	0.0	0.0	16.168825447175962	0.0
20	0.0	0.0	0.0	16.90163597380092	0.0
21	0.0	0.0	0.0	17.79231650365671	0.0
22	0.0	0.0	0.0	18.74687931388962	0.0
23	0.0	0.0	0.0	19.82112961494405	0.0
24	0.0	0.0	0.0	20.520897582753253	0.0
25	0.0	0.0	0.0	21.1340206185567	0.0
26	0.0	0.0	0.0	21.717038211883573	0.0
27	0.0	0.0	0.0	22.33676975945017	0.0
28	0.0	0.0	0.0	22.921255910946634	0.0
29	0.0	0.0	0.0	23.58430992451611	0.0
30	0.0	0.0	0.0	24.358240079889566	0.0
31	7.342790847945487E-4	0.0	0.0	25.0271683261374	0.0
32	7.342790847945487E-4	0.0	0.0	25.630745733838516	0.0
33	7.342790847945487E-4	0.0	0.0	26.241665932387583	0.0
34	7.342790847945487E-4	0.0	0.0	26.867271712632537	0.0
35	7.342790847945487E-4	0.0	0.0	27.557494052339415	0.0
36	7.342790847945487E-4	0.0	0.0	28.125091784885598	0.0
37	7.342790847945487E-4	0.0	0.0	28.730137750756306	0.0
38	7.342790847945487E-4	0.0	0.0	29.32637236760948	0.0
39	7.342790847945487E-4	0.0	0.0	29.922606984462654	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.0113706E-4	45.000004	25
AGTCCTA	20	7.0113706E-4	45.000004	31
CTCACGA	20	7.0113706E-4	45.000004	24
GGCACCG	30	2.1513788E-6	45.000004	8
CACGACG	20	7.0113706E-4	45.000004	26
GCTTGCG	20	7.0113706E-4	45.000004	1
CTTGCGG	20	7.0113706E-4	45.000004	2
CTCATGC	30	2.1513788E-6	45.000004	23
TCACTCA	20	7.0113706E-4	45.000004	32
GGGCCAC	20	7.0113706E-4	45.000004	7
CAATGGT	20	7.0113706E-4	45.000004	13
TAAGGAT	40	6.7393557E-9	45.000004	5
CGGTCTA	20	7.0113706E-4	45.000004	31
GCGGAAG	20	7.0113706E-4	45.000004	1
ACGGTCT	20	7.0113706E-4	45.000004	30
CCGGGGT	25	3.872653E-5	45.0	5
GTATTGG	25	3.872653E-5	45.0	2
CGAATAT	55	1.8189894E-12	45.0	14
TAGGGCG	25	3.872653E-5	45.0	5
ATTGGGC	25	3.872653E-5	45.0	4
>>END_MODULE