##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936364.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260175
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75378879600269	31.0	31.0	34.0	30.0	34.0
2	31.86932641491304	33.0	31.0	34.0	30.0	34.0
3	31.282709714615162	31.0	31.0	34.0	28.0	34.0
4	35.3056596521572	37.0	35.0	37.0	33.0	37.0
5	35.58800807149034	37.0	35.0	37.0	33.0	37.0
6	35.632312866339966	37.0	35.0	37.0	33.0	37.0
7	27.146232343614873	35.0	19.0	37.0	0.0	37.0
8	31.356636879023736	35.0	26.0	37.0	17.0	37.0
9	36.01082348419333	39.0	32.0	39.0	32.0	39.0
10	36.649113096953975	38.0	35.0	39.0	32.0	39.0
11	37.03809359085231	39.0	37.0	39.0	34.0	39.0
12	37.230064379744405	39.0	37.0	39.0	34.0	39.0
13	37.29889497453637	39.0	37.0	39.0	34.0	39.0
14	38.52426251561449	40.0	38.0	41.0	34.0	41.0
15	38.57918708561545	40.0	38.0	41.0	34.0	41.0
16	38.46165081195349	40.0	38.0	41.0	34.0	41.0
17	38.38057076967426	40.0	38.0	41.0	34.0	41.0
18	38.19637551647929	40.0	37.0	41.0	34.0	41.0
19	37.99835879696358	40.0	37.0	41.0	34.0	41.0
20	37.93860670702412	40.0	36.0	41.0	34.0	41.0
21	37.89228403958874	40.0	36.0	41.0	33.0	41.0
22	37.87589891419237	39.0	36.0	41.0	33.0	41.0
23	37.809812626117036	39.0	36.0	41.0	33.0	41.0
24	37.76262131257807	39.0	36.0	41.0	33.0	41.0
25	37.659475353127704	39.0	36.0	41.0	33.0	41.0
26	37.575670221965986	39.0	35.0	41.0	33.0	41.0
27	37.463949264917844	39.0	35.0	41.0	33.0	41.0
28	37.42783511098299	39.0	35.0	41.0	33.0	41.0
29	37.41160757182666	39.0	35.0	41.0	33.0	41.0
30	37.30070145094648	39.0	35.0	41.0	33.0	41.0
31	37.1172979725185	39.0	35.0	41.0	32.0	41.0
32	36.84053425578937	39.0	35.0	41.0	31.0	41.0
33	36.556621504756414	39.0	35.0	41.0	30.0	41.0
34	36.230029787642934	39.0	35.0	41.0	29.0	41.0
35	35.90819640626501	39.0	35.0	41.0	27.0	41.0
36	35.62873450562122	39.0	35.0	41.0	25.0	41.0
37	35.440165273373694	39.0	35.0	41.0	24.0	41.0
38	35.23738253098876	39.0	35.0	40.0	24.0	41.0
39	35.10742384933218	39.0	35.0	40.0	23.0	41.0
40	34.89651196310176	39.0	34.0	40.0	22.0	41.0
41	34.738157009705006	38.0	34.0	40.0	21.0	41.0
42	34.62582492553089	38.0	34.0	40.0	20.0	41.0
43	34.54218122417603	38.0	34.0	40.0	19.0	41.0
44	34.273454405688476	38.0	33.0	40.0	18.0	41.0
45	34.16255212837513	38.0	33.0	40.0	18.0	41.0
46	33.940639953877195	38.0	33.0	40.0	15.0	41.0
47	33.739925050446814	38.0	33.0	40.0	15.0	41.0
48	33.642917267223986	38.0	33.0	40.0	15.0	41.0
49	33.4090400691842	38.0	32.0	40.0	13.0	41.0
50	33.17206879984626	38.0	32.0	40.0	12.0	41.0
51	31.12235995003363	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	9.0
10	12.0
11	11.0
12	5.0
13	3.0
14	2.0
15	12.0
16	10.0
17	32.0
18	64.0
19	146.0
20	314.0
21	569.0
22	983.0
23	1690.0
24	2682.0
25	4061.0
26	5208.0
27	5690.0
28	5552.0
29	5701.0
30	6068.0
31	6966.0
32	8523.0
33	10935.0
34	14840.0
35	19820.0
36	23184.0
37	36184.0
38	46996.0
39	53883.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.64600749495532	19.722110118189683	25.7472854809263	18.884596905928703
2	35.77015470356491	22.942634765061978	25.852983568751803	15.434226962621311
3	29.252234073219945	22.325357932161047	33.79571442298453	14.626693571634478
4	24.550014413375614	24.678773902181224	34.47986931872778	16.291342365715384
5	22.259248582684734	27.702507927356585	32.70721629672336	17.33102719323532
6	22.66551359661766	32.167579513788795	31.62909580090324	13.537811088690304
7	58.13048909387912	26.127798597098106	11.970788892091862	3.770923416930912
8	79.25357932161046	3.4092437782261937	13.506678197367156	3.830498702796195
9	72.81791102142789	7.152877870663977	15.430383395791294	4.598827712116845
10	30.322667435380033	37.50552512731815	20.933602383011436	11.23820505429038
11	21.204189487844722	25.10502546363025	36.505813394830405	17.184971653694628
12	20.988565388680698	22.471029115018737	37.221869895262806	19.318535601037762
13	20.611895839338906	23.755933506293843	38.09705006245796	17.53512059190929
14	17.37945613529355	27.145959450369944	35.9577207648698	19.516863649466707
15	15.946190064379746	27.191313538964156	38.88229076583069	17.980205630825406
16	19.063707120207553	27.082540597674644	36.36167963870472	17.49207264341309
17	19.589507062554052	26.193139233208417	34.34111655616412	19.87623714807341
18	19.260113385221487	25.960219083309312	36.211780532334004	18.567886999135197
19	18.849620447775536	28.24867877390218	33.681944844815995	19.219755933506296
20	19.872777937926394	27.996156433169983	33.6212164889017	18.509849140001922
21	19.882386855001442	27.18516383203613	35.12328240607284	17.809166906889594
22	18.811953492841358	25.039300470836938	34.95301239550302	21.195733640818677
23	17.362928797924475	26.468338618237723	34.956855962333044	21.211876621504757
24	17.84452772172576	25.240319016046893	36.36360142211973	20.55155184010762
25	18.043240126837706	27.750936869414815	33.561256846353416	20.644566157394063
26	17.749207264341308	27.99346593638897	33.26376477371	20.99356202555972
27	17.499375420390123	27.426155472278275	33.8537522821178	21.2207168252138
28	16.522725088882485	26.601710387239358	35.09560872489671	21.779955798981455
29	17.79955798981455	24.43316998174306	34.8415489574325	22.925723071009894
30	18.300374747765925	24.780628423176708	34.52214855385798	22.396848275199385
31	18.409916402421448	25.537042375324305	32.9047756317863	23.148265590467954
32	18.512155280099932	25.604304794849618	33.78456807917748	22.09897184587297
33	18.147016431248197	25.543576438935332	34.47102911501874	21.838378014797733
34	17.82108196406265	24.55193619679062	35.048332852887484	22.578648986259246
35	17.183818583645625	25.40251753627366	34.71355818199289	22.700105698087825
36	17.761506678197367	26.737388296339006	34.152013068127225	21.34909195733641
37	18.4210627462285	27.710195061016623	33.623522628999716	20.245219563755164
38	17.186509080426635	27.82127414240415	34.37340251753627	20.618814259632938
39	19.247813971365428	27.173248774863072	33.0742769289901	20.504660324781394
40	19.935043720572693	25.53089266839627	34.29922167771692	20.234841933314115
41	18.47410396848275	26.906505236859807	32.16219852022677	22.45719227443067
42	18.482559815508793	27.556452387815895	32.2736619582973	21.687325838378015
43	18.772749111175173	26.66128567310464	32.57538195445373	21.990583261266455
44	18.564812145671183	26.74545978668204	31.41923705198424	23.270491015662532
45	18.56212164889017	25.726530220044204	32.016911694052084	23.69443643701355
46	19.57451715191698	26.69933698472182	31.019890458345344	22.706255405015856
47	18.201210723551455	25.94715095608725	32.87210531373114	22.979533006630152
48	18.057845680791775	25.395983472662632	33.10387239358124	23.44229845296435
49	18.369174594023256	24.885173440953203	33.421351013740754	23.32430095128279
50	17.964831363505333	24.824445085038917	34.10819640626501	23.102527145190738
51	17.632362832708754	24.446622465648122	33.52666474488325	24.39434995675987
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	224.0
2	302.0
3	3615.0
4	6928.0
5	4851.5
6	2775.0
7	2399.5
8	2024.0
9	2040.0
10	2056.0
11	1944.0
12	1832.0
13	1853.0
14	1874.0
15	1841.0
16	1808.0
17	1715.0
18	1622.0
19	1604.0
20	1586.0
21	1719.0
22	1852.0
23	1941.0
24	2030.0
25	2353.0
26	3091.0
27	3506.0
28	4123.0
29	4740.0
30	5463.0
31	6186.0
32	7222.0
33	8258.0
34	8977.5
35	9697.0
36	10610.0
37	11523.0
38	12212.5
39	12902.0
40	13832.0
41	14762.0
42	15971.5
43	17181.0
44	18764.0
45	20347.0
46	22841.5
47	25336.0
48	25109.0
49	24882.0
50	23110.5
51	21339.0
52	18111.5
53	14884.0
54	12490.5
55	10097.0
56	8803.0
57	7509.0
58	6566.0
59	5623.0
60	4957.5
61	4292.0
62	3662.0
63	3032.0
64	2447.5
65	1863.0
66	1496.0
67	1129.0
68	884.5
69	640.0
70	507.5
71	375.0
72	282.5
73	190.0
74	162.0
75	118.5
76	103.0
77	76.5
78	50.0
79	42.5
80	35.0
81	27.5
82	20.0
83	16.0
84	12.0
85	10.5
86	9.0
87	7.0
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	260175.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.18446730619352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.96488763873977	42.68046745957438
2	9.752576960800276	10.958866828104386
3	4.722474523334632	7.959891463818794
4	2.89340135530257	6.50256855402773
5	1.9061108071177317	5.354691016224417
6	1.3369944838507837	4.5070993719885255
7	0.9654856340244541	3.797170722761252
8	0.7287453794244838	3.27553367558516
9	0.5090547187873894	2.5740871384296256
>10	1.1984666461010656	8.590058077386729
>50	0.010219618367289223	0.4348485418320028
>100	0.009538310476136607	1.325216242801403
>500	0.0013626157823052297	0.5320770010092288
>1k	6.813078911526148E-4	1.507423906456362
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3938	1.5135966176611897	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	788	0.30287306620543863	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	602	0.23138272316709907	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	474	0.1821850677428654	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	474	0.1821850677428654	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	460	0.17680407418083982	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	272	0.10454501777649658	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.687133660036514E-4	0.0	0.0	0.18871913135389642	0.0
2	7.687133660036514E-4	0.0	0.0	0.7041414432593447	0.0
3	7.687133660036514E-4	0.0	0.0	1.0819640626501392	0.0
4	7.687133660036514E-4	0.0	0.0	1.4759296627270106	0.0
5	7.687133660036514E-4	0.0	0.0	2.5940232535793215	0.0
6	7.687133660036514E-4	0.0	0.0	3.7290285384837127	0.0
7	7.687133660036514E-4	0.0	0.0	4.612280196021908	0.0
8	7.687133660036514E-4	0.0	0.0	6.167771692130297	0.0
9	7.687133660036514E-4	0.0	0.0	6.862304218314596	0.0
10	7.687133660036514E-4	0.0	0.0	8.021139617565101	0.0
11	7.687133660036514E-4	0.0	0.0	9.262996060344	0.0
12	7.687133660036514E-4	0.0	0.0	10.294993754203901	0.0
13	7.687133660036514E-4	0.0	0.0	10.771211684443163	0.0
14	7.687133660036514E-4	0.0	0.0	10.987988853656192	0.0
15	7.687133660036514E-4	0.0	0.0	11.255116748342461	0.0
16	7.687133660036514E-4	0.0	0.0	11.804746805035073	0.0
17	7.687133660036514E-4	0.0	0.0	12.49274526760834	0.0
18	7.687133660036514E-4	0.0	0.0	13.293744594984146	0.0
19	7.687133660036514E-4	0.0	0.0	13.764581531661381	0.0
20	7.687133660036514E-4	0.0	0.0	14.260401652733737	0.0
21	7.687133660036514E-4	0.0	0.0	14.84616123762852	0.0
22	7.687133660036514E-4	0.0	0.0	15.519169789564716	0.0
23	7.687133660036514E-4	0.0	0.0	16.184491207840875	0.0
24	7.687133660036514E-4	0.0	0.0	16.716825213798405	0.0
25	7.687133660036514E-4	0.0	0.0	17.1792063034496	0.0
26	7.687133660036514E-4	0.0	0.0	17.613529355241663	0.0
27	7.687133660036514E-4	0.0	0.0	18.032862496396657	0.0
28	7.687133660036514E-4	0.0	0.0	18.4840972422408	0.0
29	7.687133660036514E-4	0.0	0.0	18.954165465552034	0.0
30	7.687133660036514E-4	0.0	0.0	19.50533294897665	0.0
31	7.687133660036514E-4	0.0	0.0	19.980397809166906	0.0
32	7.687133660036514E-4	0.0	0.0	20.43778226193908	0.0
33	7.687133660036514E-4	0.0	0.0	20.87633323724416	0.0
34	7.687133660036514E-4	0.0	0.0	21.31373114250024	0.0
35	7.687133660036514E-4	0.0	0.0	21.799173633131545	0.0
36	7.687133660036514E-4	0.0	0.0	22.205438647064476	0.0
37	7.687133660036514E-4	0.0	0.0	22.670894590179685	0.0
38	7.687133660036514E-4	0.0	0.0	23.093686941481696	0.0
39	7.687133660036514E-4	0.0	0.0	23.557989814547902	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGCGG	45	3.8198777E-10	45.000004	2
AAGGATC	60	0.0	45.000004	6
TCACGAC	25	3.8824604E-5	45.0	25
GTTTGCG	25	3.8824604E-5	45.0	1
CGACGGT	25	3.8824604E-5	45.0	28
CGGTTTT	175	0.0	45.0	1
TTAGACT	20	7.0232165E-4	45.0	27
GGATCGA	25	3.8824604E-5	45.0	8
CTGCGCG	20	7.0232165E-4	45.0	28
CGCTAGG	25	3.8824604E-5	45.0	2
GACGGTC	25	3.8824604E-5	45.0	29
CTTAGCG	20	7.0232165E-4	45.0	1
ATCGCGG	20	7.0232165E-4	45.0	2
GACTAAC	20	7.0232165E-4	45.0	9
CGTTTTT	1605	0.0	44.018692	1
CGTTCTG	100	0.0	42.75	1
CACGGGA	70	0.0	41.785717	4
ACACGGG	55	6.002665E-11	40.90909	3
TGCGGGC	50	1.0750227E-9	40.5	4
ATAGCGG	45	1.9184881E-8	40.000004	2
>>END_MODULE