##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936363.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80044
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.688583778921593	31.0	31.0	34.0	30.0	34.0
2	31.807968117535356	31.0	31.0	34.0	30.0	34.0
3	31.656538903603018	31.0	31.0	34.0	30.0	34.0
4	35.48597021638099	37.0	35.0	37.0	33.0	37.0
5	35.61466193593524	37.0	35.0	37.0	33.0	37.0
6	35.621583129278896	37.0	35.0	37.0	33.0	37.0
7	26.974014292139323	35.0	17.0	37.0	0.0	37.0
8	31.240592674029283	35.0	25.0	37.0	17.0	37.0
9	35.89460796561891	39.0	32.0	39.0	32.0	39.0
10	36.454237669281895	37.0	35.0	39.0	32.0	39.0
11	36.87790465244116	39.0	37.0	39.0	33.0	39.0
12	36.94256908700215	39.0	35.0	39.0	33.0	39.0
13	36.793326170606164	39.0	35.0	39.0	33.0	39.0
14	37.82697016640847	40.0	37.0	41.0	33.0	41.0
15	37.94697916146119	40.0	37.0	41.0	33.0	41.0
16	37.910799060516716	40.0	37.0	41.0	33.0	41.0
17	37.85815301584129	40.0	36.0	41.0	33.0	41.0
18	37.78483084303633	39.0	36.0	41.0	33.0	41.0
19	37.65381540152916	39.0	36.0	41.0	33.0	41.0
20	37.704225176153116	39.0	36.0	41.0	33.0	41.0
21	37.638049073009846	39.0	36.0	41.0	33.0	41.0
22	37.66965668882115	39.0	36.0	41.0	33.0	41.0
23	37.624031782519616	39.0	36.0	41.0	33.0	41.0
24	37.595247613812404	39.0	36.0	41.0	33.0	41.0
25	37.51724051771526	39.0	35.0	41.0	33.0	41.0
26	37.38375143670981	39.0	35.0	41.0	33.0	41.0
27	37.304420068962074	39.0	35.0	41.0	32.0	41.0
28	37.30769326870222	39.0	35.0	41.0	32.0	41.0
29	37.2876667832692	39.0	35.0	41.0	32.0	41.0
30	37.18598520813553	39.0	35.0	41.0	32.0	41.0
31	37.09652191294788	39.0	35.0	41.0	32.0	41.0
32	36.94807855679376	39.0	35.0	40.0	31.0	41.0
33	36.84046274548998	39.0	35.0	40.0	31.0	41.0
34	36.6164234670931	39.0	35.0	40.0	30.0	41.0
35	36.48806906201589	39.0	35.0	40.0	30.0	41.0
36	36.30921743041327	39.0	35.0	40.0	30.0	41.0
37	36.1266178601769	38.0	35.0	40.0	30.0	41.0
38	36.05199640197891	38.0	35.0	40.0	30.0	41.0
39	36.07781969916546	38.0	35.0	40.0	30.0	41.0
40	35.80859277397431	38.0	35.0	40.0	29.0	41.0
41	35.79327619809105	38.0	35.0	40.0	29.0	41.0
42	35.66369746639349	38.0	35.0	40.0	28.0	41.0
43	35.541539653190746	38.0	35.0	40.0	27.0	41.0
44	35.22249013042826	38.0	34.0	40.0	26.0	41.0
45	35.16397231522662	38.0	34.0	40.0	26.0	41.0
46	35.06508920093948	38.0	34.0	40.0	25.0	41.0
47	34.857116086152615	38.0	34.0	40.0	24.0	41.0
48	34.72452651041927	38.0	34.0	40.0	23.0	41.0
49	34.520326320523715	38.0	33.0	40.0	23.0	41.0
50	34.17947628804158	37.0	33.0	40.0	22.0	41.0
51	31.911386237569335	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	1.0
11	1.0
12	2.0
13	0.0
14	0.0
15	3.0
16	7.0
17	19.0
18	25.0
19	53.0
20	76.0
21	127.0
22	227.0
23	322.0
24	493.0
25	666.0
26	889.0
27	1073.0
28	1308.0
29	1565.0
30	1807.0
31	2351.0
32	3028.0
33	3985.0
34	6001.0
35	7768.0
36	8027.0
37	11595.0
38	14705.0
39	13915.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.597521363250216	22.866173604517513	26.033181750037482	12.503123282194792
2	28.233221728049575	26.572884913297685	28.91659587227025	16.277297486382487
3	29.333866373494576	24.568987057118584	30.843036330018492	15.254110239368348
4	26.25181150367298	26.68032582079856	30.486982159812104	16.580880515716355
5	23.57328469341862	30.393283693968314	28.17325470990955	17.86017690270351
6	23.495827294987755	34.709659687172056	26.60911498675728	15.185398031082906
7	61.15011743541052	26.915196641846983	7.68577282494628	4.248913097796212
8	81.740042976363	4.031532657038628	8.68397381440208	5.544450552196293
9	75.9182449652691	7.0698615761331265	11.326270551196842	5.685622907400929
10	33.61651091899455	36.5923741941932	16.783269201938932	13.00784568487332
11	26.941432212283246	25.593423617010647	29.17145569936535	18.293688471340765
12	26.083154265154164	21.93168757183549	32.76822747488881	19.216930688121533
13	22.151566638348907	28.259457298485835	32.93063814901804	16.65833791414722
14	17.36295037729249	31.778771675578433	29.41632102343711	21.44195692369197
15	14.595722352706012	27.037629303882866	39.402078856628854	18.96456948678227
16	17.180550697116587	26.306781270301332	34.48103543051322	22.03163260206886
17	18.899605217130578	25.229873569536753	29.942281745040226	25.928239468292443
18	19.0832542101844	25.041227324971267	35.06321523162261	20.812303233221726
19	20.05022237769227	28.591774524011793	29.729898555794314	21.628104542501625
20	21.516915696366997	27.677277497376444	30.618160011993407	20.187646794263156
21	19.657938134026285	28.544300634650945	32.36095147668782	19.43680975463495
22	17.289240917495377	26.15436509919544	30.498225975713357	26.05816800759582
23	15.006746289540754	27.923392134326118	31.607615811303784	25.462245764829344
24	17.841437209534757	25.96696816750787	33.12553095797311	23.06606366498426
25	16.905701863974816	29.26390485233122	30.48073559542252	23.34965768827145
26	15.646394483034332	31.210334316126133	30.428264454549996	22.71500674628954
27	17.149317875168656	30.412023387137076	30.506971165858776	21.93168757183549
28	13.467592823946827	28.89410824046774	34.446054669931534	23.19224426565389
29	15.916246064664435	29.0877517365449	31.898705711858476	23.097296486932187
30	19.6516915696367	30.234620958472842	29.67742741492179	20.436260056968667
31	20.970965968717206	29.470041477187547	28.49682674529009	21.062165808805158
32	21.91544650442257	31.889960521713057	26.436709809604718	19.757883164259656
33	22.984858327919643	28.298185997701264	27.28999050522213	21.426965169156965
34	19.88031582629554	28.200739593223727	29.79611213832392	22.122832442156813
35	19.97651291789516	29.54250162410674	27.3462095847284	23.134775873269703
36	24.399080505721855	28.79791114886812	28.1332766978162	18.669731647593824
37	20.040227874668933	30.595672380190898	30.845534955774323	18.51856478936585
38	21.097146569386837	29.571235820298835	27.83344160711609	21.498176003198243
39	23.938084053770424	26.500424766378494	28.77167557843186	20.78981560141922
40	25.680875518464845	25.9107490880016	28.065813802408673	20.34256159112488
41	22.13282694518015	26.871470691119885	26.749038029084005	24.24666433461596
42	22.756234071260806	26.554145220128927	28.957823197241517	21.731797511368747
43	22.455149667682775	26.90020488731198	29.642446654340116	21.002198790665133
44	20.72485133176753	27.965868772175305	28.69047024136725	22.61880965468992
45	20.45749837589326	27.659787117085603	27.09259907051122	24.790115436509918
46	24.542751486682324	27.29373844385588	27.97461396232072	20.188896107141073
47	20.473739443306183	26.214332117335466	31.621358252960874	21.69057018639748
48	21.107141072410172	25.366048673229724	30.763080305831792	22.76372994852831
49	22.341462195792314	22.428914097246512	32.56209085003248	22.66753285692869
50	21.41072410174404	23.059817100594675	31.230323322172804	24.29913547548848
51	21.00344810354305	23.430863025336066	27.80470741092399	27.76098146019689
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	64.0
1	103.0
2	142.0
3	363.0
4	584.0
5	418.0
6	252.0
7	233.0
8	214.0
9	224.5
10	235.0
11	242.5
12	250.0
13	257.5
14	265.0
15	267.0
16	269.0
17	286.5
18	304.0
19	293.5
20	283.0
21	296.5
22	310.0
23	344.0
24	378.0
25	454.5
26	678.5
27	826.0
28	1034.0
29	1242.0
30	1483.5
31	1725.0
32	1964.5
33	2204.0
34	2463.5
35	2723.0
36	2972.5
37	3222.0
38	3495.0
39	3768.0
40	4259.5
41	4751.0
42	5523.5
43	6296.0
44	6946.0
45	7596.0
46	8623.5
47	9651.0
48	9924.0
49	10197.0
50	9125.0
51	8053.0
52	6328.5
53	4604.0
54	3696.5
55	2789.0
56	2344.5
57	1900.0
58	1636.5
59	1373.0
60	1163.0
61	953.0
62	837.0
63	721.0
64	619.0
65	517.0
66	414.5
67	312.0
68	281.5
69	251.0
70	187.5
71	124.0
72	92.0
73	60.0
74	49.0
75	28.0
76	18.0
77	21.5
78	25.0
79	19.5
80	14.0
81	8.5
82	3.0
83	3.0
84	3.0
85	2.0
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	80044.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.043775923242215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69408180106218	45.873519564239665
2	9.968049766191998	11.771025935735345
3	4.223355409322697	7.480885512967868
4	2.3952095808383236	5.656888711208835
5	1.5319184951651468	4.522512618060067
6	1.0643024904255094	3.770426265553945
7	0.7659592475825734	3.1657588326420467
8	0.6072660333044159	2.8684223676977663
9	0.46126827616851107	2.4511518664734395
>10	1.2568502570830071	10.063215231622607
>50	0.021159095237087665	0.8995052721003448
>100	0.010579547618543832	1.476687821698066
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	332	0.4147718754684923	No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	294	0.3672979861076408	No Hit
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	226	0.2823447104092749	No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT	205	0.2561091399730148	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	125	0.1561641097396432	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCG	95	0.11868472340212882	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTC	92	0.1149367847683774	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGC	89	0.11118884613462594	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	83	0.10369296886712308	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36105142171805504	0.0
2	0.0	0.0	0.0	1.6803258207985607	0.0
3	0.0	0.0	0.0	2.225026235570436	0.0
4	0.0	0.0	0.0	2.830942981360252	0.0
5	0.0	0.0	0.0	4.531257808205487	0.0
6	0.0	0.0	0.0	5.389535755334566	0.0
7	0.0	0.0	0.0	6.307780720603668	0.0
8	0.0	0.0	0.0	8.262955374544001	0.0
9	0.0	0.0	0.0	8.988806156613862	0.0
10	0.0	0.0	0.0	11.00769576732797	0.0
11	0.0	0.0	0.0	13.311428714207185	0.0
12	0.0	0.0	0.0	15.196641846984159	0.0
13	0.0	0.0	0.0	15.959972015391534	0.0
14	0.0	0.0	0.0	16.26730298335915	0.0
15	0.0	0.0	0.0	16.85073209734646	0.0
16	0.0	0.0	0.0	18.02758482834441	0.0
17	0.0	0.0	0.0	19.55924241667083	0.0
18	0.0	0.0	0.0	21.290790065463995	0.0
19	0.0	0.0	0.0	22.21278296936685	0.0
20	0.0	0.0	0.0	23.10978961571136	0.0
21	0.0	0.0	0.0	24.1767028134526	0.0
22	0.0	0.0	0.0	25.298585777822197	0.0
23	0.0	0.0	0.0	26.486682324721404	0.0
24	0.0	0.0	0.0	27.342461646094648	0.0
25	0.0	0.0	0.0	28.03707960621658	0.0
26	0.0	0.0	0.0	28.660486732297237	0.0
27	0.0	0.0	0.0	29.313877367447905	0.0
28	0.0	0.0	0.0	29.959772125331067	0.0
29	0.0	0.0	0.0	30.665633901354255	0.0
30	0.0	0.0	0.0	31.450202388686222	0.0
31	0.0	0.0	0.0	32.17855179651192	0.0
32	0.0	0.0	0.0	32.80320823547049	0.0
33	0.0	0.0	0.0	33.40287841687072	0.0
34	0.0	0.0	0.0	34.07625805806806	0.0
35	0.0	0.0	0.0	34.77712258257958	0.0
36	0.0	0.0	0.0	35.36429963520064	0.0
37	0.0	0.0	0.0	35.89775623407126	0.0
38	0.0	0.0	0.0	36.519914047274	0.0
39	0.0	0.0	0.0	37.220778571785516	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATGTA	30	2.1401866E-6	45.000004	8
GCCACCG	30	2.1401866E-6	45.000004	9
CGGACGG	30	2.1401866E-6	45.000004	2
TGTCACT	50	2.1827873E-11	45.0	3
TTAGGAT	25	3.8582442E-5	45.0	5
GGAATAA	20	6.993958E-4	45.0	8
CTCACGT	25	3.8582442E-5	45.0	45
GTACAGG	20	6.993958E-4	45.0	2
CGAAAGG	20	6.993958E-4	45.0	3
CGACGGT	25	3.8582442E-5	45.0	28
CCCTAGC	45	3.7471182E-10	45.0	21
CGGTTTT	20	6.993958E-4	45.0	1
GCAACAA	20	6.993958E-4	45.0	9
AATGGGC	20	6.993958E-4	45.0	4
GGATAGC	20	6.993958E-4	45.0	8
TGTTGTG	20	6.993958E-4	45.0	13
TCGGACG	20	6.993958E-4	45.0	1
GGGACCA	40	6.679329E-9	45.0	7
ATATAGG	25	3.8582442E-5	45.0	2
GTCACTT	50	2.1827873E-11	45.0	4
>>END_MODULE