##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936362.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	261771
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02893368631361	33.0	31.0	34.0	30.0	34.0
2	32.168467859312145	33.0	31.0	34.0	30.0	34.0
3	31.884223233284054	33.0	31.0	34.0	30.0	34.0
4	35.70673986041235	37.0	35.0	37.0	35.0	37.0
5	35.853180833629395	37.0	35.0	37.0	35.0	37.0
6	35.85216467828751	37.0	35.0	37.0	35.0	37.0
7	26.997780502805888	36.0	11.0	37.0	0.0	37.0
8	31.310301752294944	37.0	23.0	37.0	17.0	37.0
9	36.091595325685425	39.0	32.0	39.0	32.0	39.0
10	36.77089135160121	38.0	35.0	39.0	33.0	39.0
11	37.251124074095294	39.0	37.0	39.0	34.0	39.0
12	37.30398325253752	39.0	37.0	39.0	34.0	39.0
13	37.29234330770024	39.0	37.0	39.0	34.0	39.0
14	38.48705547978959	40.0	38.0	41.0	34.0	41.0
15	38.57826497205573	40.0	38.0	41.0	34.0	41.0
16	38.50913966787765	40.0	38.0	41.0	34.0	41.0
17	38.41407566155151	40.0	38.0	41.0	34.0	41.0
18	38.29507470269816	40.0	38.0	41.0	34.0	41.0
19	38.173334708581166	40.0	37.0	41.0	34.0	41.0
20	38.18455061867052	40.0	37.0	41.0	34.0	41.0
21	38.13033911319436	40.0	37.0	41.0	34.0	41.0
22	38.13592796757471	40.0	37.0	41.0	34.0	41.0
23	38.040921263241536	40.0	37.0	41.0	34.0	41.0
24	38.01856966585298	40.0	37.0	41.0	34.0	41.0
25	37.918424882817426	40.0	36.0	41.0	33.0	41.0
26	37.80903155811759	40.0	36.0	41.0	33.0	41.0
27	37.674138846549084	40.0	36.0	41.0	33.0	41.0
28	37.62802602274507	40.0	36.0	41.0	33.0	41.0
29	37.609471637423546	40.0	36.0	41.0	33.0	41.0
30	37.454351322338994	40.0	36.0	41.0	33.0	41.0
31	37.3256816072063	40.0	36.0	41.0	32.0	41.0
32	37.15655286490864	40.0	35.0	41.0	31.0	41.0
33	36.96238697181888	40.0	35.0	41.0	31.0	41.0
34	36.74168261572137	39.0	35.0	41.0	30.0	41.0
35	36.54007510381211	39.0	35.0	41.0	30.0	41.0
36	36.300017954624465	39.0	35.0	41.0	29.0	41.0
37	36.18679303666182	39.0	35.0	41.0	29.0	41.0
38	36.01807304858063	39.0	35.0	41.0	28.0	41.0
39	35.94102478884216	39.0	35.0	41.0	27.0	41.0
40	35.758915235071875	39.0	35.0	41.0	26.0	41.0
41	35.627177953249216	39.0	35.0	41.0	25.0	41.0
42	35.518430231003435	39.0	35.0	40.0	25.0	41.0
43	35.43555626864703	39.0	35.0	40.0	24.0	41.0
44	35.21324363661368	39.0	34.0	40.0	24.0	41.0
45	35.103269651718485	38.0	34.0	40.0	23.0	41.0
46	34.95998792838015	38.0	34.0	40.0	23.0	41.0
47	34.80962749884441	38.0	34.0	40.0	23.0	41.0
48	34.7307150142682	38.0	34.0	40.0	23.0	41.0
49	34.58329226690504	38.0	34.0	40.0	22.0	41.0
50	34.35761791795119	38.0	33.0	40.0	21.0	41.0
51	32.30333765008347	36.0	30.0	39.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	19.0
10	25.0
11	19.0
12	12.0
13	8.0
14	8.0
15	12.0
16	28.0
17	46.0
18	99.0
19	182.0
20	378.0
21	535.0
22	857.0
23	1279.0
24	1882.0
25	2706.0
26	3577.0
27	4082.0
28	4259.0
29	4446.0
30	5298.0
31	6244.0
32	7856.0
33	10493.0
34	15589.0
35	19577.0
36	23708.0
37	38602.0
38	51258.0
39	58658.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.83050452494738	21.028303364391014	25.739673225834796	14.401518884826814
2	33.0044198937239	24.162722379484357	26.717627239075377	16.11523048771636
3	30.67184676683055	24.12872319699279	29.443674050983493	15.755755985193165
4	27.64171737893044	26.14002315000516	28.805711862658583	17.41254760840582
5	24.621902349763726	29.978492651974438	26.907869855713585	18.491735142548258
6	26.16179790733122	34.22151422426472	25.986071795577047	13.630616072827012
7	62.37321934056867	27.112246963949403	7.007269712840612	3.5072639826413163
8	84.68050318790088	4.005027294849315	8.196095060186192	3.1183744570636165
9	77.54029285138537	8.225510083240694	10.030140848298704	4.204056217075229
10	31.58638657452506	40.45979119153764	15.939504375962196	12.0143178579751
11	30.881572061076284	25.36797429814609	26.284424172272715	17.466029468504914
12	27.843038380874884	23.86169590978374	28.842767151441528	19.452498557899844
13	25.628125346199543	24.00113075932781	30.702789843030743	19.66795405144191
14	21.40382242494394	27.023619881499478	30.893032459668945	20.679525233887635
15	20.726130854831133	25.593362137135127	33.08196859086759	20.59853841716615
16	24.91376050059021	26.452510018298437	30.159948963024934	18.473780518086418
17	24.194811495543814	25.057779509571343	29.440999957978537	21.306409036906302
18	24.32851614579155	24.760191159448524	30.192038079084387	20.719254615675535
19	23.754350176299134	26.41583674280191	28.901979210836952	20.927833870062003
20	25.085284466193734	26.67904389714674	29.126985036539573	19.108686600119952
21	24.806032753819178	26.005554473184578	30.27187885594661	18.916533917049634
22	24.562308277081875	23.638982163799657	30.26118248392679	21.537527075191676
23	21.702938828212446	25.903556925709875	30.734878959090196	21.658625286987483
24	21.342700299116405	25.202944558411744	31.25670910834279	22.19764603412907
25	21.7850716847932	27.058001077277467	29.33212617134824	21.824801066581095
26	22.35121537527075	26.844073636881088	29.15792811273976	21.646782875108396
27	21.55089754021645	26.143079256296538	30.09424267776033	22.211780525726684
28	19.8077709142724	26.554125552486717	31.31324707473326	22.32485645850763
29	21.928326667201485	25.4424668889984	30.7608558625669	21.868350581233216
30	22.299643581603767	25.30494210588644	30.70660997589496	21.68880433661483
31	22.282834997001196	25.69917981747405	29.61558002987344	22.402405155651316
32	23.15191522361148	25.163215176623844	30.11410736865428	21.5707622311104
33	22.827967956725534	25.194540266110454	29.411202921637614	22.566288855526395
34	21.201737396426648	25.243437966772486	31.77662919116327	21.7781954456376
35	21.302970917328505	25.129598007418696	31.612363478001765	21.95506759725103
36	22.166320944642454	26.50981201126175	29.92119065901112	21.402676385084675
37	21.79118389737595	26.595765000706724	29.966650240095348	21.646400861821974
38	21.573436324115352	26.46931860290101	29.588839099823893	22.368405973159746
39	22.368405973159746	25.318312570911218	29.45780854258111	22.855472913347928
40	22.764553751179466	25.02607240679831	30.385336802013974	21.82403704000825
41	21.710579093940886	25.465005672897302	29.78633996890412	23.03807526425769
42	22.575839187686945	25.259482524802213	29.47767323347506	22.68700505403578
43	22.170141077506674	25.639203731505784	30.065973694565095	22.124681496422447
44	22.505930756271702	25.69688773775552	28.848497350737855	22.94868415523492
45	22.063941383881332	25.437500716274915	29.22439842457721	23.27415947526655
46	21.82021690714403	25.62430521333532	29.313025507027135	23.242452372493517
47	21.277758040424647	25.6636525818368	30.38610082858682	22.67248854915174
48	21.26018542924923	25.365300205141132	30.647397916499536	22.7271164491101
49	22.154860546049793	24.681878435731992	30.31581038388515	22.84745063433306
50	20.488518590676584	25.78933495306967	30.441874768404446	23.280271687849304
51	21.026393297958904	25.06236366900841	29.54337951873966	24.367863514293028
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	86.0
1	224.5
2	363.0
3	1938.0
4	3513.0
5	2434.0
6	1355.0
7	1199.5
8	1044.0
9	1013.5
10	983.0
11	936.5
12	890.0
13	864.5
14	839.0
15	799.5
16	760.0
17	751.0
18	742.0
19	776.0
20	810.0
21	840.5
22	871.0
23	1063.0
24	1255.0
25	1525.5
26	2177.0
27	2558.0
28	3027.5
29	3497.0
30	4099.5
31	4702.0
32	5553.0
33	6404.0
34	7432.0
35	8460.0
36	9124.0
37	9788.0
38	10606.0
39	11424.0
40	12517.5
41	13611.0
42	14994.5
43	16378.0
44	17409.5
45	18441.0
46	19745.0
47	21049.0
48	22011.5
49	22974.0
50	22156.5
51	21339.0
52	19637.5
53	17936.0
54	16297.0
55	14658.0
56	13586.5
57	12515.0
58	11599.5
59	10684.0
60	9661.0
61	8638.0
62	7603.5
63	6569.0
64	5762.5
65	4956.0
66	4200.0
67	3444.0
68	2852.5
69	2261.0
70	1905.5
71	1550.0
72	1289.5
73	1029.0
74	824.0
75	530.0
76	441.0
77	338.5
78	236.0
79	178.5
80	121.0
81	106.5
82	92.0
83	70.5
84	49.0
85	35.0
86	21.0
87	14.5
88	8.0
89	6.0
90	4.0
91	3.5
92	3.0
93	3.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	261771.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.987606572192064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.53847659966603	34.06523533294023
2	10.648639148246362	10.433026902470704
3	5.919644881892946	8.69967703563786
4	3.759601802300308	7.366955758367669
5	2.726113738862323	6.677289365521752
6	1.903163710985201	5.593886106972965
7	1.4743259358327474	5.055658923262746
8	1.1022581940457434	4.3197592600710255
9	0.7676599446403859	3.3845241014346565
>10	2.1476971541781036	13.166711338542203
>50	0.006985625259281167	0.22813937457495534
>100	0.004657083506187445	0.3467718493539321
>500	0.0	0.0
>1k	7.761805843645741E-4	0.662364650849287
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1742	0.6654671449473013	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	311	0.11880613207727365	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08404292301286238	0.0
2	0.0	0.0	0.0	0.31821706758961077	0.0
3	0.0	0.0	0.0	0.49088707305240076	0.0
4	0.0	0.0	0.0	0.6513326533496835	0.0
5	0.0	0.0	0.0	1.1494779788441043	0.0
6	0.0	0.0	0.0	1.5998716435357623	0.0
7	0.0	0.0	0.0	2.008243846720989	0.0
8	0.0	0.0	0.0	2.777618605575102	0.0
9	0.0	0.0	0.0	3.1474074668316963	0.0
10	0.0	0.0	0.0	3.854896073285429	0.0
11	0.0	0.0	0.0	4.594091782512196	0.0
12	0.0	0.0	0.0	5.204931027501137	0.0
13	0.0	0.0	0.0	5.511687696498084	0.0
14	0.0	0.0	0.0	5.673279316654633	0.0
15	0.0	0.0	0.0	5.879184478036146	0.0
16	0.0	0.0	0.0	6.24515320642852	0.0
17	0.0	0.0	0.0	6.696310897693022	0.0
18	0.0	0.0	0.0	7.176883612012026	0.0
19	0.0	0.0	0.0	7.464539616687868	0.0
20	0.0	0.0	0.0	7.804149428317117	0.0
21	0.0	0.0	0.0	8.186162714739218	0.0
22	0.0	0.0	0.0	8.600647130507198	0.0
23	0.0	0.0	0.0	8.99335678894912	0.0
24	0.0	0.0	0.0	9.31157385653873	0.0
25	0.0	0.0	0.0	9.627498844409809	0.0
26	0.0	0.0	0.0	9.912480756080697	0.0
27	0.0	0.0	0.0	10.210833132776358	0.0
28	0.0	0.0	0.0	10.532106306657345	0.0
29	0.0	0.0	0.0	10.85070538753338	0.0
30	0.0	0.0	0.0	11.212853983061532	0.0
31	3.8201328642210175E-4	0.0	0.0	11.518082598912791	0.0
32	3.8201328642210175E-4	0.0	0.0	11.832097520351757	0.0
33	3.8201328642210175E-4	0.0	0.0	12.152988680946324	0.0
34	3.8201328642210175E-4	0.0	0.0	12.465857562526026	0.0
35	3.8201328642210175E-4	0.0	0.0	12.806231400728118	0.0
36	3.8201328642210175E-4	0.0	0.0	13.116044176016441	0.0
37	3.8201328642210175E-4	0.0	0.0	13.435025270178897	0.0
38	3.8201328642210175E-4	0.0	0.0	13.77348904194888	0.0
39	3.8201328642210175E-4	0.0	0.0	14.190647550721813	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	25	3.8825263E-5	45.0	24
CGTTGAT	25	3.8825263E-5	45.0	25
GACGGTC	45	3.8198777E-10	45.0	29
ACGTAAG	25	3.8825263E-5	45.0	2
CGGTTTT	85	0.0	42.35294	1
TAGGAGT	75	0.0	42.000004	6
GGATTAT	65	0.0	41.538464	8
CGTTTTT	815	0.0	41.13497	1
ACTAACC	55	6.002665E-11	40.909092	14
CGTTTTA	110	0.0	40.909092	1
CGACGGT	50	1.0750227E-9	40.5	28
CACGACG	50	1.0750227E-9	40.5	26
CGGTCTA	50	1.0750227E-9	40.5	31
ACGACGG	50	1.0750227E-9	40.5	27
GCGATTG	45	1.9184881E-8	40.0	9
GTTTGCG	40	3.444602E-7	39.375	1
AGGATAC	35	6.2298186E-6	38.571426	7
TTACACG	170	0.0	38.38235	34
TTAATCG	30	1.1375147E-4	37.500004	20
ATCGTTG	30	1.1375147E-4	37.500004	23
>>END_MODULE