##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936361.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102296
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.791673183702198	31.0	31.0	34.0	30.0	34.0
2	31.907288652537733	33.0	31.0	34.0	30.0	34.0
3	31.74357746148432	33.0	31.0	34.0	30.0	34.0
4	35.55000195511066	37.0	35.0	37.0	33.0	37.0
5	35.67109173379213	37.0	35.0	37.0	35.0	37.0
6	35.67339876437006	37.0	35.0	37.0	35.0	37.0
7	27.01886681786189	35.0	17.0	37.0	0.0	37.0
8	31.269472902166264	35.0	25.0	37.0	17.0	37.0
9	35.966860874325484	39.0	32.0	39.0	32.0	39.0
10	36.56698209118636	38.0	35.0	39.0	32.0	39.0
11	37.00250254164386	39.0	37.0	39.0	34.0	39.0
12	37.07874208180183	39.0	37.0	39.0	34.0	39.0
13	37.011525377336355	39.0	37.0	39.0	33.0	39.0
14	38.10570305779307	40.0	37.0	41.0	33.0	41.0
15	38.224143661531244	40.0	37.0	41.0	33.0	41.0
16	38.13891061234066	40.0	37.0	41.0	33.0	41.0
17	38.04187847032142	40.0	37.0	41.0	33.0	41.0
18	37.95204113552827	40.0	37.0	41.0	33.0	41.0
19	37.83765738640807	39.0	37.0	41.0	33.0	41.0
20	37.8954504574959	39.0	36.0	41.0	33.0	41.0
21	37.84517478689294	39.0	36.0	41.0	33.0	41.0
22	37.848312739501054	39.0	36.0	41.0	33.0	41.0
23	37.78171189489325	39.0	36.0	41.0	33.0	41.0
24	37.766462031751	39.0	36.0	41.0	33.0	41.0
25	37.663388597794636	39.0	36.0	41.0	33.0	41.0
26	37.53728396027215	39.0	36.0	41.0	33.0	41.0
27	37.4663036677876	39.0	36.0	41.0	33.0	41.0
28	37.49138773754594	39.0	36.0	41.0	33.0	41.0
29	37.49906154688355	39.0	36.0	41.0	33.0	41.0
30	37.41168765152108	39.0	36.0	41.0	33.0	41.0
31	37.27059709079534	39.0	36.0	41.0	32.0	41.0
32	37.11531242668335	39.0	35.0	41.0	32.0	41.0
33	37.00313795260812	39.0	35.0	41.0	31.0	41.0
34	36.83267185422695	39.0	35.0	41.0	31.0	41.0
35	36.75063541096426	39.0	35.0	40.0	31.0	41.0
36	36.531056932822395	39.0	35.0	40.0	30.0	41.0
37	36.421424102604206	39.0	35.0	40.0	30.0	41.0
38	36.3264252756706	39.0	35.0	40.0	30.0	41.0
39	36.2871568780793	39.0	35.0	40.0	30.0	41.0
40	36.067334011105025	39.0	35.0	40.0	29.0	41.0
41	36.03474231641511	39.0	35.0	40.0	29.0	41.0
42	35.93826738093376	38.0	35.0	40.0	29.0	41.0
43	35.881530069601936	38.0	35.0	40.0	28.0	41.0
44	35.67345741768984	38.0	35.0	40.0	27.0	41.0
45	35.57072612809885	38.0	35.0	40.0	27.0	41.0
46	35.391158989598814	38.0	34.0	40.0	26.0	41.0
47	35.24128998201298	38.0	34.0	40.0	26.0	41.0
48	35.12338703370611	38.0	34.0	40.0	26.0	41.0
49	34.975991241104246	38.0	34.0	40.0	25.0	41.0
50	34.674278564166734	38.0	34.0	40.0	23.0	41.0
51	32.47517987018065	36.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	6.0
10	5.0
11	5.0
12	5.0
13	1.0
14	1.0
15	0.0
16	7.0
17	13.0
18	31.0
19	35.0
20	83.0
21	149.0
22	270.0
23	428.0
24	549.0
25	896.0
26	1111.0
27	1209.0
28	1426.0
29	1760.0
30	2140.0
31	2735.0
32	3525.0
33	4656.0
34	6849.0
35	9216.0
36	10334.0
37	15669.0
38	19655.0
39	19519.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.65930241651677	23.940330022679284	28.441972315633063	10.958395245170877
2	29.8017517791507	27.778212246813172	25.883710017987017	16.53632595604911
3	31.288613435520453	25.713615390631112	27.89258622038007	15.105184953468367
4	27.854461562524442	28.358880112614376	27.567060295612734	16.219598029248456
5	24.523930554469384	31.67768045671385	26.5719089700477	17.226480018769063
6	25.837764917494333	36.688629076405725	24.454524126065536	13.019081880034411
7	62.02295299913975	27.578790959568313	6.385391413153984	4.012864628137953
8	83.21635254555407	6.198678345194338	6.716782669899116	3.8681864393524674
9	77.30018769062329	9.442206928912176	8.63670133729569	4.620904043168843
10	33.677758661140224	41.82177211230156	13.548916868694768	10.951552357863454
11	29.125283491045593	28.876006881989518	25.929655118479705	16.06905450848518
12	28.15261593806209	26.937514663329942	28.263079690310473	16.64678970829749
13	24.98240400406663	29.64436537108	29.903417533432393	15.469813091420976
14	21.528701024477986	33.761828419488545	26.477086103073432	18.232384452960037
15	19.942128724485805	29.57300383201689	32.42551028388207	18.05935715961523
16	21.63134433408931	29.656096035035585	29.842809102995226	18.869750527879877
17	22.61672010635802	28.162391491358413	27.061664190193163	22.159224212090404
18	23.180769531555487	28.15554860405099	29.633612262454058	19.03006960193947
19	23.415382810667083	30.299327441933215	26.56897630405881	19.716313443340894
20	23.77903339329006	31.36290764057246	26.765464925314774	18.09259404082271
21	23.38898881676703	30.691327129115507	27.72542425901306	18.194259795104404
22	22.18464065066083	28.389184327832957	26.641315398451553	22.784859623054665
23	19.8678345194338	30.725541565652613	27.449753656056934	21.95687025885665
24	22.059513568467974	29.107687495112224	28.384296551184796	20.448502385235003
25	19.20602956127317	32.874208180183004	26.944357550637367	20.975404707906467
26	18.52858371783843	33.90064127629624	27.10760929068585	20.46316571517948
27	19.688941894111206	32.492961601626654	28.471298975522014	19.346797528740126
28	17.636075701884728	31.788144208962226	30.063736607491986	20.512043481661063
29	18.684015015249862	30.321811214514742	28.96203175099711	22.032142019238286
30	20.86298584499883	33.37667161961367	26.486861656369747	19.27348087901775
31	22.023344021271605	32.84683663095331	24.070344881520295	21.05947446625479
32	21.487643700633456	34.005239696566825	23.075193555955266	21.43192304684445
33	21.588331899585516	33.06483146946117	23.869946038945805	21.476890592007507
34	19.535465707359037	32.60538046453429	25.650074294205048	22.20907953390162
35	19.928442949870963	31.8057402048956	23.98529756784234	24.2805192773911
36	21.32439196058497	33.126417455227966	25.174004848674436	20.37518573551263
37	19.971455384374757	32.83315085633846	26.10952529913193	21.085868460154845
38	18.79545632282787	33.44119027136936	24.385117697661688	23.378235708141084
39	21.684132321889418	30.412723860170487	25.837764917494333	22.065378900445765
40	22.047782904512395	29.631657151794794	25.691131618049585	22.629428325643232
41	20.58242746539454	29.663916477672636	24.65785563462892	25.095800422303903
42	21.482755923985298	30.49874872917807	24.84652381324783	23.1719715335888
43	22.28239618362399	30.188863689684837	25.26491749433018	22.26382263236099
44	20.211933995464143	31.807695315554863	25.345077031359974	22.635293657621023
45	20.815085633846873	31.69038867599906	23.753616954719636	23.740908735434427
46	21.551184797059513	33.1459685618206	23.800539610541957	21.50230703057793
47	19.31258309220302	30.371666536325957	26.693125830922032	23.622624540548994
48	20.113200907171347	29.999217955736295	26.324587471650894	23.562993665441464
49	21.392820833659183	28.138930163447252	26.994212872448582	23.474036130444983
50	19.84241808086338	28.806600453585673	26.960975991241103	24.390005474309845
51	19.996871822945177	28.357902557284742	25.03616954719637	26.609056072573704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	49.0
1	97.5
2	146.0
3	408.0
4	670.0
5	487.0
6	304.0
7	274.5
8	245.0
9	256.5
10	268.0
11	277.5
12	287.0
13	285.0
14	283.0
15	282.5
16	282.0
17	275.0
18	268.0
19	301.0
20	334.0
21	349.0
22	364.0
23	396.0
24	428.0
25	521.5
26	792.0
27	969.0
28	1140.0
29	1311.0
30	1619.5
31	1928.0
32	2298.5
33	2669.0
34	3297.5
35	3926.0
36	4310.5
37	4695.0
38	4974.5
39	5254.0
40	6026.5
41	6799.0
42	7445.0
43	8091.0
44	8811.5
45	9532.0
46	10615.5
47	11699.0
48	11650.5
49	11602.0
50	10505.0
51	9408.0
52	7821.0
53	6234.0
54	5228.0
55	4222.0
56	3609.5
57	2997.0
58	2493.5
59	1990.0
60	1723.5
61	1457.0
62	1258.0
63	1059.0
64	859.5
65	660.0
66	549.5
67	439.0
68	373.0
69	307.0
70	244.0
71	181.0
72	154.0
73	127.0
74	96.0
75	59.0
76	53.0
77	41.0
78	29.0
79	26.0
80	23.0
81	18.0
82	13.0
83	10.0
84	7.0
85	5.5
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	102296.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.510557597560016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.08181061005253	44.33017908813639
2	9.78395743740545	11.253616954719638
3	4.371844775713484	7.542816923437867
4	2.5224796450850744	5.802768436693516
5	1.6589893083578384	4.770470008602487
6	1.130356444731519	3.900445765230312
7	0.7903996192483556	3.1819425979510445
8	0.6340194795261002	2.917025103620865
9	0.44704322551036024	2.3138734652381325
>10	1.5519029083306422	12.118753421443653
>50	0.013598273019326547	0.51908188003441
>100	0.013598273019326547	1.349026354891687
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	378	0.36951591460076644	No Hit
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	209	0.20430906389301637	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	183	0.17889262532259326	No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT	137	0.13392508015953702	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGC	126	0.1231719715335888	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCG	121	0.11828419488543053	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC	117	0.11437397356690389	No Hit
TCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	109	0.10655353092985063	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20137639790412137	0.0
2	0.0	0.0	0.0	0.8905529052944396	0.0
3	0.0	0.0	0.0	1.1779541722061468	0.0
4	0.0	0.0	0.0	1.5474700868069133	0.0
5	0.0	0.0	0.0	2.603229842809103	0.0
6	0.0	0.0	0.0	3.184875263939939	0.0
7	0.0	0.0	0.0	3.8144208962227264	0.0
8	0.0	0.0	0.0	5.153671697818097	0.0
9	0.0	0.0	0.0	5.691327129115508	0.0
10	0.0	0.0	0.0	7.1214905763666225	0.0
11	0.0	0.0	0.0	8.737389536247752	0.0
12	0.0	0.0	0.0	10.23500430124345	0.0
13	0.0	0.0	0.0	10.814694611715023	0.0
14	0.0	0.0	0.0	11.070814108078517	0.0
15	0.0	0.0	0.0	11.487252678501603	0.0
16	0.0	0.0	0.0	12.298623602095878	0.0
17	0.0	0.0	0.0	13.359271134746226	0.0
18	0.0	0.0	0.0	14.516696645030109	0.0
19	0.0	0.0	0.0	15.180456713850004	0.0
20	0.0	0.0	0.0	15.901892547118166	0.0
21	0.0	0.0	0.0	16.755298349886605	0.0
22	0.0	0.0	0.0	17.647806365840307	0.0
23	0.0	0.0	0.0	18.554000156408854	0.0
24	0.0	0.0	0.0	19.29694220692891	0.0
25	0.0	0.0	0.0	19.888363181356066	0.0
26	0.0	0.0	0.0	20.502267928364745	0.0
27	0.0	0.0	0.0	21.072182685540003	0.0
28	0.0	0.0	0.0	21.699773207163524	0.0
29	0.0	0.0	0.0	22.32149839680926	0.0
30	0.0	0.0	0.0	23.064440447329318	0.0
31	0.0	0.0	0.0	23.70180652224916	0.0
32	0.0	0.0	0.0	24.306913271291155	0.0
33	0.0	0.0	0.0	24.89246891374052	0.0
34	0.0	0.0	0.0	25.50050832877141	0.0
35	0.0	0.0	0.0	26.13103151638383	0.0
36	0.0	0.0	0.0	26.731250488777665	0.0
37	0.0	0.0	0.0	27.351998123093768	0.0
38	0.0	0.0	0.0	27.972745757409868	0.0
39	0.0	0.0	0.0	28.565144287166653	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	3.8658407E-5	45.0	25
TTGATTC	20	7.0031435E-4	45.0	43
GTTGAAT	25	3.8658407E-5	45.0	35
TAATATC	35	1.1974771E-7	45.0	21
GATACTA	20	7.0031435E-4	45.0	1
GGTGTTC	40	6.710252E-9	45.0	9
AGGAGCC	20	7.0031435E-4	45.0	7
CGACGGT	25	3.8658407E-5	45.0	28
ACCGATT	20	7.0031435E-4	45.0	10
ACCTGTA	20	7.0031435E-4	45.0	14
GTCTCAC	25	3.8658407E-5	45.0	22
CTAAGGA	35	1.1974771E-7	45.0	4
CACGACG	25	3.8658407E-5	45.0	26
AGGCGAT	55	1.8189894E-12	45.0	7
ATATCAG	20	7.0031435E-4	45.0	1
CTAAAGC	20	7.0031435E-4	45.0	5
CTACGAA	20	7.0031435E-4	45.0	11
TACGAAT	20	7.0031435E-4	45.0	12
TACTGGA	20	7.0031435E-4	45.0	15
GGGAACG	25	3.8658407E-5	45.0	7
>>END_MODULE