##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936361.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 102296 Sequences flagged as poor quality 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.791673183702198 31.0 31.0 34.0 30.0 34.0 2 31.907288652537733 33.0 31.0 34.0 30.0 34.0 3 31.74357746148432 33.0 31.0 34.0 30.0 34.0 4 35.55000195511066 37.0 35.0 37.0 33.0 37.0 5 35.67109173379213 37.0 35.0 37.0 35.0 37.0 6 35.67339876437006 37.0 35.0 37.0 35.0 37.0 7 27.01886681786189 35.0 17.0 37.0 0.0 37.0 8 31.269472902166264 35.0 25.0 37.0 17.0 37.0 9 35.966860874325484 39.0 32.0 39.0 32.0 39.0 10 36.56698209118636 38.0 35.0 39.0 32.0 39.0 11 37.00250254164386 39.0 37.0 39.0 34.0 39.0 12 37.07874208180183 39.0 37.0 39.0 34.0 39.0 13 37.011525377336355 39.0 37.0 39.0 33.0 39.0 14 38.10570305779307 40.0 37.0 41.0 33.0 41.0 15 38.224143661531244 40.0 37.0 41.0 33.0 41.0 16 38.13891061234066 40.0 37.0 41.0 33.0 41.0 17 38.04187847032142 40.0 37.0 41.0 33.0 41.0 18 37.95204113552827 40.0 37.0 41.0 33.0 41.0 19 37.83765738640807 39.0 37.0 41.0 33.0 41.0 20 37.8954504574959 39.0 36.0 41.0 33.0 41.0 21 37.84517478689294 39.0 36.0 41.0 33.0 41.0 22 37.848312739501054 39.0 36.0 41.0 33.0 41.0 23 37.78171189489325 39.0 36.0 41.0 33.0 41.0 24 37.766462031751 39.0 36.0 41.0 33.0 41.0 25 37.663388597794636 39.0 36.0 41.0 33.0 41.0 26 37.53728396027215 39.0 36.0 41.0 33.0 41.0 27 37.4663036677876 39.0 36.0 41.0 33.0 41.0 28 37.49138773754594 39.0 36.0 41.0 33.0 41.0 29 37.49906154688355 39.0 36.0 41.0 33.0 41.0 30 37.41168765152108 39.0 36.0 41.0 33.0 41.0 31 37.27059709079534 39.0 36.0 41.0 32.0 41.0 32 37.11531242668335 39.0 35.0 41.0 32.0 41.0 33 37.00313795260812 39.0 35.0 41.0 31.0 41.0 34 36.83267185422695 39.0 35.0 41.0 31.0 41.0 35 36.75063541096426 39.0 35.0 40.0 31.0 41.0 36 36.531056932822395 39.0 35.0 40.0 30.0 41.0 37 36.421424102604206 39.0 35.0 40.0 30.0 41.0 38 36.3264252756706 39.0 35.0 40.0 30.0 41.0 39 36.2871568780793 39.0 35.0 40.0 30.0 41.0 40 36.067334011105025 39.0 35.0 40.0 29.0 41.0 41 36.03474231641511 39.0 35.0 40.0 29.0 41.0 42 35.93826738093376 38.0 35.0 40.0 29.0 41.0 43 35.881530069601936 38.0 35.0 40.0 28.0 41.0 44 35.67345741768984 38.0 35.0 40.0 27.0 41.0 45 35.57072612809885 38.0 35.0 40.0 27.0 41.0 46 35.391158989598814 38.0 34.0 40.0 26.0 41.0 47 35.24128998201298 38.0 34.0 40.0 26.0 41.0 48 35.12338703370611 38.0 34.0 40.0 26.0 41.0 49 34.975991241104246 38.0 34.0 40.0 25.0 41.0 50 34.674278564166734 38.0 34.0 40.0 23.0 41.0 51 32.47517987018065 36.0 30.0 39.0 18.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 3.0 9 6.0 10 5.0 11 5.0 12 5.0 13 1.0 14 1.0 15 0.0 16 7.0 17 13.0 18 31.0 19 35.0 20 83.0 21 149.0 22 270.0 23 428.0 24 549.0 25 896.0 26 1111.0 27 1209.0 28 1426.0 29 1760.0 30 2140.0 31 2735.0 32 3525.0 33 4656.0 34 6849.0 35 9216.0 36 10334.0 37 15669.0 38 19655.0 39 19519.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.65930241651677 23.940330022679284 28.441972315633063 10.958395245170877 2 29.8017517791507 27.778212246813172 25.883710017987017 16.53632595604911 3 31.288613435520453 25.713615390631112 27.89258622038007 15.105184953468367 4 27.854461562524442 28.358880112614376 27.567060295612734 16.219598029248456 5 24.523930554469384 31.67768045671385 26.5719089700477 17.226480018769063 6 25.837764917494333 36.688629076405725 24.454524126065536 13.019081880034411 7 62.02295299913975 27.578790959568313 6.385391413153984 4.012864628137953 8 83.21635254555407 6.198678345194338 6.716782669899116 3.8681864393524674 9 77.30018769062329 9.442206928912176 8.63670133729569 4.620904043168843 10 33.677758661140224 41.82177211230156 13.548916868694768 10.951552357863454 11 29.125283491045593 28.876006881989518 25.929655118479705 16.06905450848518 12 28.15261593806209 26.937514663329942 28.263079690310473 16.64678970829749 13 24.98240400406663 29.64436537108 29.903417533432393 15.469813091420976 14 21.528701024477986 33.761828419488545 26.477086103073432 18.232384452960037 15 19.942128724485805 29.57300383201689 32.42551028388207 18.05935715961523 16 21.63134433408931 29.656096035035585 29.842809102995226 18.869750527879877 17 22.61672010635802 28.162391491358413 27.061664190193163 22.159224212090404 18 23.180769531555487 28.15554860405099 29.633612262454058 19.03006960193947 19 23.415382810667083 30.299327441933215 26.56897630405881 19.716313443340894 20 23.77903339329006 31.36290764057246 26.765464925314774 18.09259404082271 21 23.38898881676703 30.691327129115507 27.72542425901306 18.194259795104404 22 22.18464065066083 28.389184327832957 26.641315398451553 22.784859623054665 23 19.8678345194338 30.725541565652613 27.449753656056934 21.95687025885665 24 22.059513568467974 29.107687495112224 28.384296551184796 20.448502385235003 25 19.20602956127317 32.874208180183004 26.944357550637367 20.975404707906467 26 18.52858371783843 33.90064127629624 27.10760929068585 20.46316571517948 27 19.688941894111206 32.492961601626654 28.471298975522014 19.346797528740126 28 17.636075701884728 31.788144208962226 30.063736607491986 20.512043481661063 29 18.684015015249862 30.321811214514742 28.96203175099711 22.032142019238286 30 20.86298584499883 33.37667161961367 26.486861656369747 19.27348087901775 31 22.023344021271605 32.84683663095331 24.070344881520295 21.05947446625479 32 21.487643700633456 34.005239696566825 23.075193555955266 21.43192304684445 33 21.588331899585516 33.06483146946117 23.869946038945805 21.476890592007507 34 19.535465707359037 32.60538046453429 25.650074294205048 22.20907953390162 35 19.928442949870963 31.8057402048956 23.98529756784234 24.2805192773911 36 21.32439196058497 33.126417455227966 25.174004848674436 20.37518573551263 37 19.971455384374757 32.83315085633846 26.10952529913193 21.085868460154845 38 18.79545632282787 33.44119027136936 24.385117697661688 23.378235708141084 39 21.684132321889418 30.412723860170487 25.837764917494333 22.065378900445765 40 22.047782904512395 29.631657151794794 25.691131618049585 22.629428325643232 41 20.58242746539454 29.663916477672636 24.65785563462892 25.095800422303903 42 21.482755923985298 30.49874872917807 24.84652381324783 23.1719715335888 43 22.28239618362399 30.188863689684837 25.26491749433018 22.26382263236099 44 20.211933995464143 31.807695315554863 25.345077031359974 22.635293657621023 45 20.815085633846873 31.69038867599906 23.753616954719636 23.740908735434427 46 21.551184797059513 33.1459685618206 23.800539610541957 21.50230703057793 47 19.31258309220302 30.371666536325957 26.693125830922032 23.622624540548994 48 20.113200907171347 29.999217955736295 26.324587471650894 23.562993665441464 49 21.392820833659183 28.138930163447252 26.994212872448582 23.474036130444983 50 19.84241808086338 28.806600453585673 26.960975991241103 24.390005474309845 51 19.996871822945177 28.357902557284742 25.03616954719637 26.609056072573704 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 49.0 1 97.5 2 146.0 3 408.0 4 670.0 5 487.0 6 304.0 7 274.5 8 245.0 9 256.5 10 268.0 11 277.5 12 287.0 13 285.0 14 283.0 15 282.5 16 282.0 17 275.0 18 268.0 19 301.0 20 334.0 21 349.0 22 364.0 23 396.0 24 428.0 25 521.5 26 792.0 27 969.0 28 1140.0 29 1311.0 30 1619.5 31 1928.0 32 2298.5 33 2669.0 34 3297.5 35 3926.0 36 4310.5 37 4695.0 38 4974.5 39 5254.0 40 6026.5 41 6799.0 42 7445.0 43 8091.0 44 8811.5 45 9532.0 46 10615.5 47 11699.0 48 11650.5 49 11602.0 50 10505.0 51 9408.0 52 7821.0 53 6234.0 54 5228.0 55 4222.0 56 3609.5 57 2997.0 58 2493.5 59 1990.0 60 1723.5 61 1457.0 62 1258.0 63 1059.0 64 859.5 65 660.0 66 549.5 67 439.0 68 373.0 69 307.0 70 244.0 71 181.0 72 154.0 73 127.0 74 96.0 75 59.0 76 53.0 77 41.0 78 29.0 79 26.0 80 23.0 81 18.0 82 13.0 83 10.0 84 7.0 85 5.5 86 4.0 87 2.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 102296.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.510557597560016 #Duplication Level Percentage of deduplicated Percentage of total 1 77.08181061005253 44.33017908813639 2 9.78395743740545 11.253616954719638 3 4.371844775713484 7.542816923437867 4 2.5224796450850744 5.802768436693516 5 1.6589893083578384 4.770470008602487 6 1.130356444731519 3.900445765230312 7 0.7903996192483556 3.1819425979510445 8 0.6340194795261002 2.917025103620865 9 0.44704322551036024 2.3138734652381325 >10 1.5519029083306422 12.118753421443653 >50 0.013598273019326547 0.51908188003441 >100 0.013598273019326547 1.349026354891687 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 378 0.36951591460076644 No Hit GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC 209 0.20430906389301637 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 183 0.17889262532259326 No Hit CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT 137 0.13392508015953702 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGC 126 0.1231719715335888 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCG 121 0.11828419488543053 No Hit GAATCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTC 117 0.11437397356690389 No Hit TCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC 109 0.10655353092985063 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.20137639790412137 0.0 2 0.0 0.0 0.0 0.8905529052944396 0.0 3 0.0 0.0 0.0 1.1779541722061468 0.0 4 0.0 0.0 0.0 1.5474700868069133 0.0 5 0.0 0.0 0.0 2.603229842809103 0.0 6 0.0 0.0 0.0 3.184875263939939 0.0 7 0.0 0.0 0.0 3.8144208962227264 0.0 8 0.0 0.0 0.0 5.153671697818097 0.0 9 0.0 0.0 0.0 5.691327129115508 0.0 10 0.0 0.0 0.0 7.1214905763666225 0.0 11 0.0 0.0 0.0 8.737389536247752 0.0 12 0.0 0.0 0.0 10.23500430124345 0.0 13 0.0 0.0 0.0 10.814694611715023 0.0 14 0.0 0.0 0.0 11.070814108078517 0.0 15 0.0 0.0 0.0 11.487252678501603 0.0 16 0.0 0.0 0.0 12.298623602095878 0.0 17 0.0 0.0 0.0 13.359271134746226 0.0 18 0.0 0.0 0.0 14.516696645030109 0.0 19 0.0 0.0 0.0 15.180456713850004 0.0 20 0.0 0.0 0.0 15.901892547118166 0.0 21 0.0 0.0 0.0 16.755298349886605 0.0 22 0.0 0.0 0.0 17.647806365840307 0.0 23 0.0 0.0 0.0 18.554000156408854 0.0 24 0.0 0.0 0.0 19.29694220692891 0.0 25 0.0 0.0 0.0 19.888363181356066 0.0 26 0.0 0.0 0.0 20.502267928364745 0.0 27 0.0 0.0 0.0 21.072182685540003 0.0 28 0.0 0.0 0.0 21.699773207163524 0.0 29 0.0 0.0 0.0 22.32149839680926 0.0 30 0.0 0.0 0.0 23.064440447329318 0.0 31 0.0 0.0 0.0 23.70180652224916 0.0 32 0.0 0.0 0.0 24.306913271291155 0.0 33 0.0 0.0 0.0 24.89246891374052 0.0 34 0.0 0.0 0.0 25.50050832877141 0.0 35 0.0 0.0 0.0 26.13103151638383 0.0 36 0.0 0.0 0.0 26.731250488777665 0.0 37 0.0 0.0 0.0 27.351998123093768 0.0 38 0.0 0.0 0.0 27.972745757409868 0.0 39 0.0 0.0 0.0 28.565144287166653 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGAC 25 3.8658407E-5 45.0 25 TTGATTC 20 7.0031435E-4 45.0 43 GTTGAAT 25 3.8658407E-5 45.0 35 TAATATC 35 1.1974771E-7 45.0 21 GATACTA 20 7.0031435E-4 45.0 1 GGTGTTC 40 6.710252E-9 45.0 9 AGGAGCC 20 7.0031435E-4 45.0 7 CGACGGT 25 3.8658407E-5 45.0 28 ACCGATT 20 7.0031435E-4 45.0 10 ACCTGTA 20 7.0031435E-4 45.0 14 GTCTCAC 25 3.8658407E-5 45.0 22 CTAAGGA 35 1.1974771E-7 45.0 4 CACGACG 25 3.8658407E-5 45.0 26 AGGCGAT 55 1.8189894E-12 45.0 7 ATATCAG 20 7.0031435E-4 45.0 1 CTAAAGC 20 7.0031435E-4 45.0 5 CTACGAA 20 7.0031435E-4 45.0 11 TACGAAT 20 7.0031435E-4 45.0 12 TACTGGA 20 7.0031435E-4 45.0 15 GGGAACG 25 3.8658407E-5 45.0 7 >>END_MODULE