FastQCFastQC Report
Sat 14 Jan 2017
SRR2936358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248917
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15060.6050209507586867No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.10204204614389537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG502.1827873E-1145.0000045
ACGGGCG351.2068085E-745.0000045
CGAACGG207.022628E-445.02
TATGCGG302.1586275E-644.9999962
CGTTTTT5500.042.5454561
GGCCGAT556.002665E-1140.9090928
GCGAGAC403.4436925E-739.37521
CACGACC403.4436925E-739.37527
AGACACG403.4436925E-739.37524
GCGATGC403.4436925E-739.3759
ACGACCA403.4436925E-739.37528
ACACGCG2450.037.6530636
TACGCGG301.1373542E-437.4999962
GCTACGA601.546141E-1037.49999610
GCCGATG552.730303E-936.818189
TAGGGCG552.730303E-936.818185
GGGCGAT2900.036.4655157
CTCACGA250.00210394536.00000424
GCCCACC250.00210394536.00000412
CGCGCAA250.00210394536.00000418
Produced by FastQC (version 0.11.5)