SRR2936357.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936357.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197746
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	1947	0.9845964014442771	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG	1933	0.9775166122197162	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC	1781	0.9006503292101989	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1136	0.5744743256500764	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTT	582	0.294316952049599	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	533	0.2695376897636362	RNA PCR Primer, Index 33 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT	421	0.21289937596714975	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG	417	0.21087657904584667	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC	390	0.19722269982705085	No Hit
GAATCTATCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	378	0.19115430906314163	RNA PCR Primer, Index 33 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	371	0.18761441445086122	RNA PCR Primer, Index 33 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTC	366	0.18508591829923235	No Hit
GAATCTATCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTT	232	0.11732222143557897	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	215	0.10872533452004086	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	201	0.10164554529548005	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAG	20	7.019105E-4	45.000004	2
GTCTAAT	20	7.019105E-4	45.000004	33
TACAGCG	20	7.019105E-4	45.000004	1
TAGGCGA	35	1.2051169E-7	45.0	6
GGCACCG	60	0.0	44.999996	8
TACGGCT	210	0.0	43.92857	7
TACGGGA	120	0.0	43.124996	4
GAATGAC	65	0.0	41.538464	1
GTAGGGC	60	3.6379788E-12	41.249996	4
AGGCGAT	60	3.6379788E-12	41.249996	7
ACGGCTG	290	0.0	41.120693	8
CGTTTTT	575	0.0	41.086956	1
TGATACC	285	0.0	41.05263	4
ATACGGC	275	0.0	40.909092	6
TAAGGAT	50	1.0713848E-9	40.5	5
CGACGGT	45	1.9144863E-8	40.0	28
AGCGACT	45	1.9144863E-8	40.0	19
CACAACG	45	1.9144863E-8	40.0	12
GCGATTT	40	3.4388904E-7	39.375004	9
CGGCTGT	305	0.0	39.098362	9