##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936357.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197746
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.985309437359035	33.0	31.0	34.0	30.0	34.0
2	32.09602722684656	33.0	31.0	34.0	30.0	34.0
3	31.867972044946548	33.0	31.0	34.0	30.0	34.0
4	35.66086798215893	37.0	35.0	37.0	33.0	37.0
5	35.780526534038614	37.0	35.0	37.0	35.0	37.0
6	35.75570681581423	37.0	35.0	37.0	35.0	37.0
7	26.75782569558929	35.0	10.0	37.0	0.0	37.0
8	31.030958906880542	35.0	19.0	37.0	17.0	37.0
9	35.873974694810514	39.0	32.0	39.0	32.0	39.0
10	36.69970568304795	38.0	35.0	39.0	33.0	39.0
11	37.11202755049407	39.0	37.0	39.0	34.0	39.0
12	37.09452024314019	39.0	37.0	39.0	33.0	39.0
13	37.01712297593883	39.0	37.0	39.0	33.0	39.0
14	38.2326924438421	40.0	38.0	41.0	34.0	41.0
15	38.23112983322039	40.0	37.0	41.0	33.0	41.0
16	38.222350894581936	40.0	37.0	41.0	33.0	41.0
17	38.07932903826121	40.0	37.0	41.0	33.0	41.0
18	37.960312724404034	40.0	37.0	41.0	33.0	41.0
19	37.80302509279581	40.0	37.0	41.0	33.0	41.0
20	37.89176013674107	40.0	36.0	41.0	33.0	41.0
21	37.934006250442486	40.0	36.0	41.0	33.0	41.0
22	37.9880452701951	40.0	37.0	41.0	33.0	41.0
23	37.99517562934269	40.0	36.0	41.0	34.0	41.0
24	37.898890495888665	40.0	36.0	41.0	33.0	41.0
25	37.82069422390339	40.0	36.0	41.0	33.0	41.0
26	37.689485501603066	40.0	36.0	41.0	33.0	41.0
27	37.65131532369808	40.0	36.0	41.0	33.0	41.0
28	37.627764910541806	40.0	36.0	41.0	33.0	41.0
29	37.56865372750903	40.0	36.0	41.0	33.0	41.0
30	37.46246194613292	40.0	36.0	41.0	33.0	41.0
31	37.325857413045014	40.0	36.0	41.0	32.0	41.0
32	37.17786453329018	39.0	35.0	41.0	32.0	41.0
33	37.040673389095105	39.0	35.0	41.0	31.0	41.0
34	36.720469693445125	39.0	35.0	41.0	30.0	41.0
35	36.642799348659395	39.0	35.0	41.0	30.0	41.0
36	36.41156331860063	39.0	35.0	41.0	30.0	41.0
37	36.27791206901783	39.0	35.0	41.0	30.0	41.0
38	36.15329260768865	39.0	35.0	41.0	29.0	41.0
39	35.87075339071334	39.0	35.0	40.0	27.0	41.0
40	35.68773072527384	39.0	35.0	40.0	26.0	41.0
41	35.55554094646668	39.0	35.0	40.0	26.0	41.0
42	35.555348780759154	39.0	35.0	40.0	26.0	41.0
43	35.56533128356578	38.0	35.0	40.0	26.0	41.0
44	35.365362636918064	38.0	35.0	40.0	26.0	41.0
45	35.30091632700535	38.0	34.0	40.0	25.0	41.0
46	35.10129155583425	38.0	34.0	40.0	24.0	41.0
47	34.83300294316952	38.0	34.0	40.0	23.0	41.0
48	34.76681197091218	38.0	34.0	40.0	23.0	41.0
49	34.635330171027476	38.0	34.0	40.0	23.0	41.0
50	34.44086353200571	38.0	33.0	40.0	22.0	41.0
51	32.12446269456778	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	5.0
10	9.0
11	9.0
12	7.0
13	4.0
14	5.0
15	8.0
16	19.0
17	53.0
18	93.0
19	158.0
20	215.0
21	353.0
22	569.0
23	848.0
24	1244.0
25	1772.0
26	2428.0
27	2973.0
28	3263.0
29	3785.0
30	4376.0
31	5513.0
32	6614.0
33	8959.0
34	12727.0
35	15595.0
36	18001.0
37	28749.0
38	37328.0
39	42044.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75838702173495	19.82694972338252	24.86523115511818	12.549432099764344
2	29.951048314504465	29.013481941480485	25.152974017173545	15.882495726841503
3	28.730796071728378	29.116138885236616	27.09435336239418	15.05871168064082
4	25.804820325063467	24.533492460024476	32.21354667098197	17.448140543930094
5	26.719630232722785	28.432433525836174	26.41671639375765	18.43121984768339
6	25.044754381883834	35.73827030635259	25.64198517289857	13.57499013886501
7	59.79842828679215	27.187402020774126	9.526362100876883	3.4878075915568454
8	80.47292992020067	6.9179654708565534	8.946325083693223	3.6627795252495625
9	74.27356305563703	7.2406015798043954	9.748364062989896	8.737471301568679
10	33.97590848866728	36.08922557219868	16.448878864806368	13.485987074327674
11	31.362454866343693	24.258897777957582	25.799763332760207	18.578884022938517
12	28.407148564319883	22.23104386435124	29.64459458092705	19.717212990401826
13	25.007838338070048	23.464444287115796	33.18853478705005	18.339182587764103
14	21.611056607971843	26.86274311490498	30.19782953890344	21.32837073821974
15	20.09851021006746	24.694810514498396	36.43967513881444	18.767004136619704
16	23.904402617499215	26.19876002548724	28.619036541826382	21.27780081518716
17	24.32716717405156	24.440443801644534	29.616781123259127	21.615607901044775
18	24.303905009456575	24.872816643573067	30.155350803556075	20.66792754341428
19	23.139279682016323	26.322150637686732	27.78463281178886	22.753936868508088
20	24.159275029583405	27.24505173303126	30.12399745127588	18.471675786109454
21	24.033861620462613	27.082216580866366	30.08556430977112	18.798357488899903
22	23.037128437490516	22.64672863167902	30.1027580836022	24.213384847228262
23	21.28032931133879	26.826332770321525	30.978629150526434	20.914708767813256
24	23.347122065680214	25.08521032030989	28.683766043308083	22.88390157070181
25	21.519019348052552	29.757365509289695	27.005350297856843	21.718264844800906
26	21.238356275221747	25.459933449981285	28.601842767995304	24.699867506801652
27	22.251777532794595	26.353503989966924	28.461258381964743	22.933460095273738
28	19.39862247529659	25.632882586752704	31.521750123896314	23.446744814054394
29	22.55974836406299	24.996712955002884	30.146753916640538	22.29678476429359
30	22.997178198294783	27.037715048597693	29.726012157009496	20.239094596098024
31	25.60810332446674	24.962831106571056	27.763899143345505	21.6651664256167
32	24.826798013613423	26.696873767358127	26.67816289583607	21.79816532319238
33	24.984576173475066	24.978507782711155	25.782063859698805	24.254852184114974
34	22.16075167133596	27.327480707574363	30.0066752298403	20.50509239124938
35	20.68562701647568	29.808946830782922	27.183862126161845	22.32156402657955
36	24.243726801047806	26.293325781558156	27.830651441748504	21.632295975645523
37	21.06237294306838	28.920433283100543	28.35152164898405	21.665672124847028
38	24.703407401413934	26.991190719407726	25.839207872725616	22.46619400645272
39	23.47809816633459	26.047555955619835	27.21572117767237	23.258624700373208
40	23.82652493602905	26.701930759661387	28.6938800279146	20.777664276394972
41	20.08182213546671	28.35759003974796	27.355294165242285	24.205293659543052
42	21.262629838277388	25.92163684726872	30.7758437591658	22.0398895552881
43	22.39489041497679	24.946648731200632	27.725466001840744	24.932994851981835
44	22.772142040799814	25.0735792380124	28.409171361241185	23.745107359946598
45	20.781709870237577	25.311257876265515	27.627866050387873	26.27916620310904
46	24.01869064355284	26.50774225521629	27.074631092411476	22.398936008819394
47	19.677262751206094	25.794200641226624	32.60242937910249	21.926107228464797
48	21.14884751145409	26.72721572117767	27.945445167032457	24.178491600335782
49	21.272238123653576	24.47685414622799	31.32301032637828	22.927897403740154
50	21.511433859597666	24.88191922971893	29.834737491529538	23.771909419153864
51	21.157444398369627	24.726163866778595	27.999049285446986	26.11734244940479
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	71.0
1	185.5
2	300.0
3	1297.5
4	2295.0
5	1594.5
6	894.0
7	747.5
8	601.0
9	563.0
10	525.0
11	529.0
12	533.0
13	517.5
14	502.0
15	519.5
16	537.0
17	499.5
18	462.0
19	473.0
20	484.0
21	551.0
22	618.0
23	755.0
24	892.0
25	1081.5
26	1569.0
27	1867.0
28	2131.0
29	2395.0
30	2912.5
31	3430.0
32	4002.0
33	4574.0
34	5410.5
35	6247.0
36	6630.5
37	7014.0
38	7624.0
39	8234.0
40	8870.0
41	9506.0
42	10396.5
43	11287.0
44	12710.0
45	14133.0
46	18768.0
47	23403.0
48	19857.5
49	16312.0
50	15778.5
51	15245.0
52	13896.5
53	12548.0
54	11673.0
55	10798.0
56	10205.0
57	9612.0
58	8815.5
59	8019.0
60	7262.0
61	6505.0
62	5775.0
63	5045.0
64	4407.5
65	3770.0
66	3208.0
67	2646.0
68	2213.0
69	1780.0
70	1519.0
71	1258.0
72	1054.0
73	850.0
74	710.0
75	428.0
76	286.0
77	232.5
78	179.0
79	134.0
80	89.0
81	73.5
82	58.0
83	52.5
84	47.0
85	38.0
86	29.0
87	20.5
88	12.0
89	8.5
90	5.0
91	4.5
92	4.0
93	2.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	197746.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.21635835870258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.7944217701962	31.332112912524146
2	11.574443873029074	10.69857291677202
3	6.461248919477848	8.958461865221041
4	4.144828265365299	7.662354737896089
5	2.849295882526726	6.584203978841543
6	1.9980085566412447	5.5404407674491525
7	1.4180827433773566	4.587703417515399
8	1.0438664638749986	3.859496525846288
9	0.7167007692223523	2.9810969627704225
>10	1.9673709665065486	11.684686415907276
>50	0.012036196124344848	0.38787130965986666
>100	0.013130395772012561	1.7219058792592519
>500	0.002188399295335427	0.5638546418132352
>1k	0.004376798590670854	3.4372376685242685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	1947	0.9845964014442771	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG	1933	0.9775166122197162	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC	1781	0.9006503292101989	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1136	0.5744743256500764	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTT	582	0.294316952049599	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	533	0.2695376897636362	RNA PCR Primer, Index 33 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT	421	0.21289937596714975	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG	417	0.21087657904584667	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC	390	0.19722269982705085	No Hit
GAATCTATCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	378	0.19115430906314163	RNA PCR Primer, Index 33 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	371	0.18761441445086122	RNA PCR Primer, Index 33 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTC	366	0.18508591829923235	No Hit
GAATCTATCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTT	232	0.11732222143557897	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	215	0.10872533452004086	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	201	0.10164554529548005	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05410981764485755	0.0
2	0.0	0.0	0.0	0.500136538792188	0.0
3	0.0	0.0	0.0	0.6073447756212516	0.0
4	0.0	0.0	0.0	1.113044005947023	0.0
5	0.0	0.0	0.0	2.931032738968171	0.0
6	0.0	0.0	0.0	3.2936190871117494	0.0
7	0.0	0.0	0.0	3.788192934370354	0.0
8	0.0	0.0	0.0	4.539156291404124	0.0
9	0.0	0.0	0.0	4.767226644281047	0.0
10	0.0	0.0	0.0	6.597352158830014	0.0
11	0.0	0.0	0.0	7.097994396852528	0.0
12	0.0	0.0	0.0	8.804729299202007	0.0
13	0.0	0.0	0.0	9.061118808977172	0.0
14	0.0	0.0	0.0	9.185015120406987	0.0
15	0.0	0.0	0.0	9.417636766356841	0.0
16	0.0	0.0	0.0	9.699816936878623	0.0
17	0.0	0.0	0.0	9.994639588158547	0.0
18	0.0	0.0	0.0	10.340537861701375	0.0
19	0.0	0.0	0.0	10.556977132280805	0.0
20	0.0	0.0	0.0	10.784541785927402	0.0
21	0.0	0.0	0.0	11.085432827971236	0.0
22	0.0	0.0	0.0	11.390875163088001	0.0
23	0.0	0.0	0.0	11.677606626682714	0.0
24	0.0	0.0	0.0	11.896068694183448	0.0
25	0.0	0.0	0.0	12.08216601094333	0.0
26	0.0	0.0	0.0	12.283434304612989	0.0
27	0.0	0.0	0.0	12.513021755180889	0.0
28	0.0	0.0	0.0	12.720358439614454	0.0
29	0.0	0.0	0.0	12.965622566322454	0.0
30	0.0	0.0	0.0	13.277133292203128	0.0
31	0.0	0.0	0.0	13.520880321220151	0.0
32	0.0	0.0	0.0	13.779798327146946	0.0
33	0.0	0.0	0.0	13.999271793108331	0.0
34	0.0	0.0	0.0	14.272855076714574	0.0
35	0.0	0.0	0.0	14.54239276647821	0.0
36	0.0	0.0	0.0	14.789679690107512	0.0
37	0.0	0.0	0.0	15.065285770635057	0.0
38	0.0	0.0	0.0	15.397024465728764	0.0
39	0.0	0.0	0.0	15.932559950643755	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAG	20	7.019105E-4	45.000004	2
GTCTAAT	20	7.019105E-4	45.000004	33
TACAGCG	20	7.019105E-4	45.000004	1
TAGGCGA	35	1.2051169E-7	45.0	6
GGCACCG	60	0.0	44.999996	8
TACGGCT	210	0.0	43.92857	7
TACGGGA	120	0.0	43.124996	4
GAATGAC	65	0.0	41.538464	1
GTAGGGC	60	3.6379788E-12	41.249996	4
AGGCGAT	60	3.6379788E-12	41.249996	7
ACGGCTG	290	0.0	41.120693	8
CGTTTTT	575	0.0	41.086956	1
TGATACC	285	0.0	41.05263	4
ATACGGC	275	0.0	40.909092	6
TAAGGAT	50	1.0713848E-9	40.5	5
CGACGGT	45	1.9144863E-8	40.0	28
AGCGACT	45	1.9144863E-8	40.0	19
CACAACG	45	1.9144863E-8	40.0	12
GCGATTT	40	3.4388904E-7	39.375004	9
CGGCTGT	305	0.0	39.098362	9
>>END_MODULE