##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936356.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	167425
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9495893683739	33.0	31.0	34.0	30.0	34.0
2	32.08325518889055	33.0	31.0	34.0	30.0	34.0
3	31.985276989696878	33.0	31.0	34.0	30.0	34.0
4	35.737243541884425	37.0	35.0	37.0	35.0	37.0
5	35.80816484993281	37.0	35.0	37.0	35.0	37.0
6	35.78613110347917	37.0	35.0	37.0	35.0	37.0
7	26.79835150067194	35.0	10.0	37.0	0.0	37.0
8	31.131366283410483	35.0	19.0	37.0	17.0	37.0
9	35.91466029565477	39.0	32.0	39.0	32.0	39.0
10	36.59845303867403	37.0	35.0	39.0	32.0	39.0
11	36.95954009257877	39.0	37.0	39.0	33.0	39.0
12	37.032271166193816	39.0	37.0	39.0	34.0	39.0
13	37.032211437957294	39.0	37.0	39.0	33.0	39.0
14	38.18522920710766	40.0	37.0	41.0	33.0	41.0
15	38.28517246528296	40.0	37.0	41.0	34.0	41.0
16	38.209437061370764	40.0	37.0	41.0	34.0	41.0
17	38.09696281917277	40.0	37.0	41.0	33.0	41.0
18	38.029410183664325	40.0	37.0	41.0	33.0	41.0
19	37.95881738091683	40.0	37.0	41.0	33.0	41.0
20	38.0298521726146	40.0	37.0	41.0	34.0	41.0
21	37.990407645214276	40.0	37.0	41.0	33.0	41.0
22	37.96321935194863	40.0	37.0	41.0	33.0	41.0
23	37.925333731521576	40.0	37.0	41.0	33.0	41.0
24	37.87279080185157	40.0	36.0	41.0	33.0	41.0
25	37.79262356278931	40.0	36.0	41.0	33.0	41.0
26	37.69542183067045	40.0	36.0	41.0	33.0	41.0
27	37.59124384052561	40.0	36.0	41.0	33.0	41.0
28	37.54573092429446	40.0	36.0	41.0	33.0	41.0
29	37.527961773928624	40.0	36.0	41.0	33.0	41.0
30	37.44911452889353	40.0	36.0	41.0	33.0	41.0
31	37.30401075108257	39.0	36.0	41.0	32.0	41.0
32	37.17446020606241	39.0	35.0	41.0	32.0	41.0
33	37.10126325220248	39.0	35.0	41.0	31.0	41.0
34	36.99329251903838	39.0	35.0	41.0	31.0	41.0
35	36.95105868299238	39.0	35.0	41.0	31.0	41.0
36	36.81577422726594	39.0	35.0	41.0	31.0	41.0
37	36.77042257727341	39.0	35.0	41.0	31.0	41.0
38	36.65085859340003	39.0	35.0	41.0	30.0	41.0
39	36.61653874869344	39.0	35.0	41.0	30.0	41.0
40	36.47490518142452	39.0	35.0	40.0	30.0	41.0
41	36.42596983724056	39.0	35.0	40.0	30.0	41.0
42	36.333850978049874	39.0	35.0	40.0	30.0	41.0
43	36.31355532327908	39.0	35.0	40.0	30.0	41.0
44	36.12493355233687	39.0	35.0	40.0	29.0	41.0
45	36.03245035090339	38.0	35.0	40.0	29.0	41.0
46	35.88022696729879	38.0	35.0	40.0	29.0	41.0
47	35.729216066895624	38.0	35.0	40.0	28.0	41.0
48	35.66785127669105	38.0	35.0	40.0	28.0	41.0
49	35.46076153501568	38.0	34.0	40.0	27.0	41.0
50	35.2672181573839	38.0	34.0	40.0	26.0	41.0
51	33.0295893683739	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	10.0
10	9.0
11	8.0
12	6.0
13	7.0
14	5.0
15	6.0
16	11.0
17	26.0
18	50.0
19	80.0
20	155.0
21	254.0
22	400.0
23	565.0
24	799.0
25	1090.0
26	1419.0
27	1720.0
28	2050.0
29	2570.0
30	3186.0
31	4226.0
32	5502.0
33	7693.0
34	11693.0
35	14508.0
36	15948.0
37	25321.0
38	32690.0
39	35407.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.193370165745854	22.057040465880245	24.229953710616694	12.519635657757206
2	30.822756458115574	25.17545169478871	25.731521576825443	18.27027027027027
3	30.838883081977002	24.916231148275347	27.604001791847093	16.640883977900554
4	28.659997013588175	25.75302374197402	26.445274003285054	19.141705241152753
5	25.007615350156787	30.77557115126176	24.298043900253845	19.91876959832761
6	25.586979244437806	34.77437658653128	25.094818575481558	14.54382559354935
7	61.72853516499926	27.793937583992832	6.3138718829326566	4.163655368075258
8	83.1064655816037	5.276989696879199	7.308944303419442	4.307600418097655
9	76.87621322980439	7.672689263849485	9.542780349410183	5.908317156935942
10	40.820068687472	28.615798118560548	17.00582350306107	13.558309690906375
11	28.545318799462443	28.630730177691504	24.993280573391072	17.83067044945498
12	26.852620576377483	24.361953113334327	29.022547409287743	19.762878901000448
13	23.53770344930566	25.076302822159175	31.556816484993284	19.829177243541885
14	19.129759593847993	28.673137225623414	30.71464835000747	21.482454830521128
15	18.346722413020757	28.324324324324323	33.935194863371656	19.39375839928326
16	21.340898909959684	27.84709571449903	30.44228759145886	20.369717784082425
17	21.13722562341347	27.053904733462748	28.573391070628638	23.235478572495147
18	22.229655069434077	26.90936240107511	30.437509332536955	20.42347319695386
19	22.734358668060324	28.197103180528593	27.700761535015676	21.3677766163954
20	24.222786322233837	27.77124085411378	28.237121098999552	19.76885172465283
21	24.219799910407644	27.578318650141853	28.72987905032104	19.47200238912946
22	22.623264148126026	25.147379423622517	29.490816783634465	22.738539644616992
23	21.729132447364492	25.67477975212782	29.57802000895924	23.018067791548454
24	21.77452590712259	25.41615648797969	30.215917575033597	22.59340002986412
25	21.4788711363297	27.30297147976706	28.619381812751975	22.59877557115126
26	20.489174257130056	28.337464536359562	28.12065103777811	23.05271016873227
27	19.93071524563237	28.858294758847247	28.002986411826193	23.208003583694193
28	18.97685530834702	28.275347170374793	30.588024488576977	22.15977303270121
29	19.995221741078094	26.183067044945496	29.315215768254443	24.506495445721967
30	20.577572047185306	27.562789308645662	28.480215021651485	23.379423622517546
31	22.272659399731225	27.854263102881887	27.58130506196805	22.291772435418842
32	23.07301776915037	28.48499328057339	25.794833507540687	22.647155442735553
33	21.55233686725399	28.842765417351053	26.16634313871883	23.438554576676125
34	20.8278333582201	27.001343885321788	28.820068687472002	23.350754068986113
35	20.87979692399582	26.30252351799313	27.981484246677617	24.836195311333434
36	21.519486337165894	27.672689263849488	27.4397491414066	23.36807525757802
37	20.650440495744363	28.50948185754816	28.660594295953413	22.17948335075407
38	20.795580110497237	29.852172614603557	26.54920113483649	22.803046140062715
39	21.53859937285352	27.151859041361803	28.450948185754815	22.858593400029864
40	22.588024488576973	25.936986710467373	28.52142750485292	22.95356129610273
41	20.669553531431983	25.872480215021653	28.272958040913842	25.18500821263252
42	21.062565327758698	25.319396744811108	27.872181573839033	25.74585635359116
43	21.953710616694043	25.395848887561595	27.948036434224278	24.702404061520085
44	19.86322233836046	27.268329102583245	27.202628042407046	25.665820516649244
45	20.744213827086753	26.490070180677915	27.154845453187992	25.610870539047337
46	21.99671494699119	26.92130804837987	27.94385545766761	23.138121546961326
47	19.74257130058235	26.09765566671644	29.969837240555474	24.189935792145736
48	20.185754815589068	25.48066298342541	29.380319546065405	24.953262654920113
49	20.363744960430044	24.84694639390772	30.272062117365984	24.51724652829625
50	20.076452142750487	24.887561594743914	30.019411676870238	25.01657458563536
51	19.825593549350458	25.049425115723455	28.167836344631926	26.957144990294164
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	170.0
2	266.0
3	429.5
4	593.0
5	433.5
6	274.0
7	244.0
8	214.0
9	214.0
10	214.0
11	227.5
12	241.0
13	264.0
14	287.0
15	286.0
16	285.0
17	301.5
18	318.0
19	379.5
20	441.0
21	514.0
22	587.0
23	800.5
24	1014.0
25	1242.5
26	1640.5
27	1810.0
28	2277.0
29	2744.0
30	3147.0
31	3550.0
32	3990.0
33	4430.0
34	4856.0
35	5282.0
36	5893.5
37	6505.0
38	7020.5
39	7536.0
40	8283.0
41	9030.0
42	10289.0
43	11548.0
44	12269.5
45	12991.0
46	14085.0
47	15179.0
48	15571.0
49	15963.0
50	14982.0
51	14001.0
52	12393.5
53	10786.0
54	9781.5
55	8777.0
56	8013.0
57	7249.0
58	6771.5
59	6294.0
60	5697.5
61	5101.0
62	4562.5
63	4024.0
64	3475.5
65	2927.0
66	2424.0
67	1921.0
68	1585.5
69	1250.0
70	1083.0
71	916.0
72	754.0
73	592.0
74	464.5
75	258.0
76	179.0
77	153.5
78	128.0
79	88.0
80	48.0
81	30.0
82	12.0
83	14.5
84	17.0
85	11.0
86	5.0
87	6.5
88	8.0
89	6.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	167425.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.14603553830074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.17344033134869	32.38226071375242
2	9.659590991457415	8.91503658354487
3	5.20062127879886	7.199641630580857
4	3.4610406419880926	6.388532178587428
5	2.593838985244629	5.984769299686427
6	1.947967900595392	5.393459758100642
7	1.6166192078695314	5.222039719277288
8	1.1894900336526015	4.391219949230999
9	1.0548796272327208	4.381066149021951
>10	3.0779187160238157	18.270867552635508
>50	0.014237639140564329	0.398387337613857
>100	0.010354646647683147	1.0727191279677468
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	284	0.16962819172763924	No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	277	0.16544721517097208	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	269	0.16066895624906674	No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	256	0.15290428550097057	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG	242	0.14454233238763625	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGC	182	0.10870539047334628	No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT	171	0.10213528445572645	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16186352097954307	0.0
2	0.0	0.0	0.0	0.7973719575929521	0.0
3	0.0	0.0	0.0	1.087651187098701	0.0
4	0.0	0.0	0.0	1.4292967000149321	0.0
5	0.0	0.0	0.0	2.5055995221741076	0.0
6	0.0	0.0	0.0	3.0646558160370314	0.0
7	0.0	0.0	0.0	3.561594743915186	0.0
8	0.0	0.0	0.0	4.518441093026729	0.0
9	0.0	0.0	0.0	4.8320143347767655	0.0
10	0.0	0.0	0.0	5.819919366880693	0.0
11	0.0	0.0	0.0	6.963715096311781	0.0
12	0.0	0.0	0.0	7.972525011199044	0.0
13	0.0	0.0	0.0	8.398984619979094	0.0
14	0.0	0.0	0.0	8.572196505898164	0.0
15	0.0	0.0	0.0	8.849335523368673	0.0
16	0.0	0.0	0.0	9.458563535911603	0.0
17	0.0	0.0	0.0	10.163356726892639	0.0
18	0.0	0.0	0.0	10.899805883231297	0.0
19	0.0	0.0	0.0	11.371658951769449	0.0
20	0.0	0.0	0.0	11.836344631924742	0.0
21	0.0	0.0	0.0	12.401373749440047	0.0
22	0.0	0.0	0.0	12.995669702852023	0.0
23	0.0	0.0	0.0	13.572047185306854	0.0
24	0.0	0.0	0.0	14.063610571897865	0.0
25	0.0	0.0	0.0	14.476929968642676	0.0
26	0.0	0.0	0.0	14.847245035090339	0.0
27	0.0	0.0	0.0	15.224130207555621	0.0
28	0.0	0.0	0.0	15.627893086456622	0.0
29	0.0	0.0	0.0	16.057339107062862	0.0
30	0.0	0.0	0.0	16.497536210243393	0.0
31	0.0	0.0	0.0	16.942511572345825	0.0
32	0.0	0.0	0.0	17.355830969090636	0.0
33	0.0	0.0	0.0	17.779901448409735	0.0
34	0.0	0.0	0.0	18.184261609675975	0.0
35	0.0	0.0	0.0	18.60295654770793	0.0
36	0.0	0.0	0.0	18.963117813946543	0.0
37	0.0	0.0	0.0	19.318500821263253	0.0
38	0.0	0.0	0.0	19.72644467672092	0.0
39	0.0	0.0	0.0	20.13319396744811	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGG	20	7.016009E-4	45.0	3
GGCACCG	40	6.7557266E-9	45.0	8
CACGCCG	20	7.016009E-4	45.0	26
AGGCGAT	40	6.7557266E-9	45.0	7
GGCCCCT	20	7.016009E-4	45.0	8
TCGGCAT	20	7.016009E-4	45.0	45
GGCGATA	45	3.8016879E-10	45.0	8
CGGTAGT	20	7.016009E-4	45.0	12
CTTAGTC	25	3.8764912E-5	45.0	40
GTACGTA	20	7.016009E-4	45.0	22
TACGTAG	20	7.016009E-4	45.0	23
CGTTTTT	165	0.0	40.90909	1
ATAGCGG	45	1.911394E-8	40.0	2
AAGGATC	45	1.911394E-8	40.0	6
TTAGCGG	40	3.4346704E-7	39.375	2
CTATCTC	75	0.0	39.0	5
TCGATGG	35	6.2163617E-6	38.57143	2
ATACTCT	35	6.2163617E-6	38.57143	27
GCGGGTG	35	6.2163617E-6	38.57143	5
AGTACGG	35	6.2163617E-6	38.57143	2
>>END_MODULE