##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936353.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225264
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81998899069536	31.0	31.0	34.0	30.0	34.0
2	31.916995170111512	33.0	31.0	34.0	30.0	34.0
3	31.56827988493501	33.0	31.0	34.0	30.0	34.0
4	35.48170147027488	37.0	35.0	37.0	33.0	37.0
5	35.662804531571844	37.0	35.0	37.0	33.0	37.0
6	35.67196711414163	37.0	35.0	37.0	33.0	37.0
7	26.963811350237943	35.0	17.0	37.0	0.0	37.0
8	31.1542501242986	35.0	23.0	37.0	17.0	37.0
9	35.87299346544499	39.0	32.0	39.0	32.0	39.0
10	36.60606222032815	38.0	35.0	39.0	32.0	39.0
11	36.94340862277151	39.0	37.0	39.0	33.0	39.0
12	37.065474287946586	39.0	37.0	39.0	34.0	39.0
13	37.05542829746431	39.0	37.0	39.0	33.0	39.0
14	38.29601711769302	40.0	38.0	41.0	34.0	41.0
15	38.32746022444776	40.0	38.0	41.0	34.0	41.0
16	38.27102865970595	40.0	37.0	41.0	34.0	41.0
17	38.129732225300096	40.0	37.0	41.0	33.0	41.0
18	37.98644257404645	40.0	37.0	41.0	33.0	41.0
19	37.80873996732723	40.0	37.0	41.0	33.0	41.0
20	37.82982189786206	40.0	36.0	41.0	33.0	41.0
21	37.797402159244264	40.0	36.0	41.0	33.0	41.0
22	37.78335197812345	39.0	36.0	41.0	33.0	41.0
23	37.755797641877976	39.0	36.0	41.0	33.0	41.0
24	37.667332552027844	39.0	36.0	41.0	33.0	41.0
25	37.58419454506712	39.0	36.0	41.0	33.0	41.0
26	37.45009411179771	39.0	35.0	41.0	33.0	41.0
27	37.37684672206833	39.0	35.0	41.0	33.0	41.0
28	37.3427533915761	39.0	35.0	41.0	33.0	41.0
29	37.3279174657291	39.0	35.0	41.0	33.0	41.0
30	37.204444562824065	39.0	35.0	41.0	32.0	41.0
31	37.097503373819166	39.0	35.0	41.0	32.0	41.0
32	36.905408764827044	39.0	35.0	41.0	31.0	41.0
33	36.642827970736555	39.0	35.0	41.0	30.0	41.0
34	36.45372984586974	39.0	35.0	41.0	30.0	41.0
35	36.24382502308403	39.0	35.0	41.0	30.0	41.0
36	35.88547659634917	39.0	35.0	41.0	27.0	41.0
37	35.78246857021095	39.0	35.0	40.0	27.0	41.0
38	35.5444411890049	39.0	35.0	40.0	25.0	41.0
39	35.44245862632289	39.0	35.0	40.0	25.0	41.0
40	35.16450031962497	38.0	34.0	40.0	24.0	41.0
41	34.9297801690461	38.0	34.0	40.0	23.0	41.0
42	34.87785442858158	38.0	34.0	40.0	23.0	41.0
43	34.69895322821223	38.0	34.0	40.0	22.0	41.0
44	34.551987889764895	38.0	34.0	40.0	21.0	41.0
45	34.50012873783649	38.0	33.0	40.0	22.0	41.0
46	34.20620693941331	38.0	33.0	40.0	20.0	41.0
47	33.970119504226155	38.0	33.0	40.0	18.0	41.0
48	33.80887314439946	38.0	33.0	40.0	18.0	41.0
49	33.58338216492648	37.0	33.0	40.0	15.0	41.0
50	33.45221162724625	37.0	32.0	40.0	15.0	41.0
51	31.276937282477448	35.0	28.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	13.0
10	19.0
11	10.0
12	6.0
13	2.0
14	3.0
15	16.0
16	19.0
17	34.0
18	70.0
19	154.0
20	288.0
21	491.0
22	825.0
23	1241.0
24	2032.0
25	2848.0
26	3736.0
27	4210.0
28	4552.0
29	4869.0
30	5360.0
31	6378.0
32	7881.0
33	10195.0
34	14501.0
35	18478.0
36	20985.0
37	31822.0
38	40562.0
39	43650.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.021361602386534	19.392801335322112	23.51152425598409	15.074312806307265
2	32.56401377938774	27.431369415441438	23.646033098941686	16.358583706229133
3	30.37502663541445	25.915814333404363	28.48480005682222	15.224358974358973
4	26.19770580296896	24.126802329710916	31.83464734711272	17.8408445202074
5	25.212639392002274	28.666364798636266	27.357234178563814	18.763761630797642
6	23.42895447119824	35.38381632218197	27.321276369060303	13.865952837559487
7	57.933358193053486	27.185435755380354	10.960916968534697	3.9202890830314656
8	76.81209602954755	7.2466084238937425	11.836778180268485	4.10451736629022
9	70.99891682647915	7.829036153135877	13.013175651679806	8.158871368705164
10	36.38708359968747	29.72467859933234	20.223826266070034	13.664411534910151
11	26.804993252361676	25.767099936075006	29.17820867959372	18.2496981319696
12	25.99660842389374	22.424355422970383	32.16847787484907	19.41055827828681
13	22.230360821081042	24.46684778748491	34.36367994886	18.939111442574045
14	18.69317778251296	28.406225584203426	30.806076425882523	22.094520207401093
15	17.925189999289724	26.63630229419703	36.188205838482844	19.2503018680304
16	20.582960437531074	27.40784146601321	31.227803821294124	20.78139427516159
17	20.408498472902906	26.517774699907665	31.178528304567088	21.895198522622344
18	20.885716315079193	26.965249662618085	31.445326372611692	20.70370764969103
19	21.95601605227644	28.11589956673059	28.9566908161091	20.97139356488387
20	22.50914482562682	27.650223737481355	31.93896938703033	17.901662049861496
21	22.07765111158463	29.073442716101994	30.504652319056753	18.344253853256625
22	21.42241991618723	24.66927693728248	30.47135805099794	23.43694509553235
23	20.55854464095461	27.5752006534555	31.10661268556005	20.75964202002983
24	20.942094608992115	25.37511542012927	31.69925065700689	21.983539313871724
25	19.842939839477236	29.885822856736983	29.41659563889481	20.85464166489097
26	19.824295049364302	27.85664819944598	29.43524042900774	22.883816322181975
27	21.377583635201365	27.66709283329782	29.551104481852402	21.40421904964841
28	18.014862561261452	27.036721358050997	32.41751899992897	22.530897080758578
29	20.283312025001777	25.254368207969314	30.398554584842675	24.063765182186234
30	19.189928261950424	27.573868882733148	31.56873712621635	21.667465729100076
31	21.26837843596846	26.506676610554724	28.140759286881174	24.08418566659564
32	21.905852688401165	27.200973080474466	27.596065061439024	23.29710916968535
33	19.97478514099013	27.65821436181547	28.34185666595639	24.02514383123801
34	20.6801797002628	26.082729597272536	29.01617657504084	24.220914127423825
35	21.69676468499183	25.052382981745865	29.656758292492363	23.594094040769942
36	21.591998721500104	27.222281412032107	28.827065132466796	22.358654734000993
37	20.496839264152282	26.710881454648767	30.952127281767172	21.84015199943178
38	20.301512891540593	28.120782726045885	28.282814830598763	23.29488955181476
39	22.834984729029053	25.941117977129057	28.910966687974998	22.312930605866892
40	22.687602102422048	26.289154059237163	30.209443142268626	20.813800696072164
41	20.56609134171461	26.32156048014774	30.218321613750977	22.894026564386674
42	21.14718730023439	25.60906314368918	30.884650898501313	22.35909865757511
43	21.471251509340153	25.16203210455288	29.96439732935578	23.40231905675119
44	20.554549328787555	26.200369344413666	28.777789615739753	24.467291711059026
45	19.97966830030542	25.698735705660912	28.64772000852333	25.673875985510335
46	22.185968463669294	27.749218694509555	27.240926202145037	22.823886639676115
47	19.959691739470134	25.409297535336318	31.99889906953619	22.63211165565736
48	20.046700759997158	25.84212302010086	30.025214859009875	24.085961360892107
49	19.62586121173379	24.95782726045884	31.934530151289152	23.481781376518217
50	19.598781873712625	24.72032814830599	31.17764045741885	24.50324952056254
51	20.281536330705304	24.265306484835573	28.96201789899851	26.491139285460612
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	139.0
1	285.0
2	431.0
3	2260.0
4	4089.0
5	2840.5
6	1592.0
7	1368.0
8	1144.0
9	1112.0
10	1080.0
11	1067.5
12	1055.0
13	1035.0
14	1015.0
15	951.5
16	888.0
17	937.5
18	987.0
19	989.0
20	991.0
21	1133.5
22	1276.0
23	1277.5
24	1279.0
25	1610.5
26	2335.5
27	2729.0
28	3318.5
29	3908.0
30	4493.0
31	5078.0
32	5549.0
33	6020.0
34	6738.0
35	7456.0
36	7779.5
37	8103.0
38	8892.0
39	9681.0
40	10518.5
41	11356.0
42	12028.5
43	12701.0
44	13244.5
45	13788.0
46	14833.0
47	15878.0
48	19361.5
49	22845.0
50	20515.0
51	18185.0
52	17004.0
53	15823.0
54	13918.0
55	12013.0
56	10723.0
57	9433.0
58	8657.0
59	7881.0
60	7457.0
61	7033.0
62	6151.5
63	5270.0
64	4534.5
65	3799.0
66	3152.5
67	2506.0
68	2138.5
69	1771.0
70	1612.0
71	1453.0
72	1260.5
73	1068.0
74	868.0
75	530.0
76	392.0
77	306.5
78	221.0
79	181.0
80	141.0
81	102.0
82	63.0
83	44.5
84	26.0
85	23.0
86	20.0
87	16.5
88	13.0
89	12.0
90	11.0
91	12.0
92	13.0
93	9.5
94	6.0
95	5.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	225264.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.31418043205424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.05382873510952	33.61853673161252
2	10.659964106364562	10.087349302555095
3	5.302650835053078	7.526717351336533
4	3.418188349773768	6.469151213277673
5	2.2716034183512694	5.373952700297158
6	1.7160237151178097	4.871535340966085
7	1.3361076607079854	4.425178725477127
8	1.085834468367104	4.110029436453189
9	0.7916917183717201	3.3712420328642376
>10	2.3062756114296405	14.528514327562785
>50	0.030909876240698207	1.0015598080466648
>100	0.022933133985034153	2.0083090451505665
>500	9.970927819580068E-4	0.4439320675326951
>1k	0.0029912783458740204	2.1639919168676642
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2256	1.0014915832090348	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG	1274	0.5655586334256694	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC	1057	0.46922721784217625	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGC	941	0.4177320832445486	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	342	0.15182186234817813	No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC	312	0.13850415512465375	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	307	0.13628453725406633	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	234	0.10387811634349031	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4392357411747994E-4	0.0	0.0	0.1282939129199517	0.0
2	4.4392357411747994E-4	0.0	0.0	0.6987357056609134	0.0
3	4.4392357411747994E-4	0.0	0.0	0.9224731870161234	0.0
4	4.4392357411747994E-4	0.0	0.0	1.3091306200724484	0.0
5	4.4392357411747994E-4	0.0	0.0	2.833120250017757	0.0
6	4.4392357411747994E-4	0.0	0.0	3.4976738404716246	0.0
7	4.4392357411747994E-4	0.0	0.0	4.161339583777257	0.0
8	4.4392357411747994E-4	0.0	0.0	5.154396619078059	0.0
9	4.4392357411747994E-4	0.0	0.0	5.513086866964984	0.0
10	4.4392357411747994E-4	0.0	0.0	7.215089850131402	0.0
11	4.4392357411747994E-4	0.0	0.0	8.082960437531074	0.0
12	4.4392357411747994E-4	0.0	0.0	9.525712053412885	0.0
13	4.4392357411747994E-4	0.0	0.0	9.877299524113928	0.0
14	4.4392357411747994E-4	0.0	0.0	10.049097947297394	0.0
15	4.4392357411747994E-4	0.0	0.0	10.361176219901981	0.0
16	4.4392357411747994E-4	0.0	0.0	10.835730520633568	0.0
17	4.4392357411747994E-4	0.0	0.0	11.44656935861922	0.0
18	4.4392357411747994E-4	0.0	0.0	12.088926770367214	0.0
19	4.4392357411747994E-4	0.0	0.0	12.47380850912707	0.0
20	4.4392357411747994E-4	0.0	0.0	12.868900490091626	0.0
21	4.4392357411747994E-4	0.0	0.0	13.392286383976135	0.0
22	4.4392357411747994E-4	0.0	0.0	13.949854393067689	0.0
23	4.4392357411747994E-4	0.0	0.0	14.48877761204631	0.0
24	4.4392357411747994E-4	0.0	0.0	14.912724625328503	0.0
25	4.4392357411747994E-4	0.0	0.0	15.299825981958946	0.0
26	4.4392357411747994E-4	0.0	0.0	15.641647134029405	0.0
27	4.4392357411747994E-4	0.0	0.0	16.003444846935153	0.0
28	4.4392357411747994E-4	0.0	0.0	16.388326585695008	0.0
29	4.4392357411747994E-4	0.0	0.0	16.799399815327792	0.0
30	4.4392357411747994E-4	0.0	0.0	17.258860714539384	0.0
31	4.4392357411747994E-4	0.0	0.0	17.69656935861922	0.0
32	4.4392357411747994E-4	0.0	0.0	18.103203352510832	0.0
33	4.4392357411747994E-4	0.0	0.0	18.491636479863626	0.0
34	4.4392357411747994E-4	0.0	0.0	18.90315363307053	0.0
35	4.4392357411747994E-4	0.0	0.0	19.33509127068684	0.0
36	4.4392357411747994E-4	0.0	0.0	19.762145748987855	0.0
37	4.4392357411747994E-4	0.0	0.0	20.170999360750052	0.0
38	4.4392357411747994E-4	0.0	0.0	20.565647418140493	0.0
39	4.4392357411747994E-4	0.0	0.0	20.989150507848567	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCACTA	30	2.157707E-6	45.000004	8
GCGCGCC	35	1.2061173E-7	45.000004	16
CGCGCCT	35	1.2061173E-7	45.000004	17
ACGGGTA	30	2.157707E-6	45.000004	5
TACGAAT	60	0.0	45.000004	12
CGAATAT	60	0.0	45.000004	14
GCTACGA	60	0.0	45.000004	10
ACAACGA	40	6.7739165E-9	45.0	13
CGGGTCT	25	3.8807906E-5	45.0	6
GGCCGAT	25	3.8807906E-5	45.0	8
ACACAAC	40	6.7739165E-9	45.0	11
CCTCGTG	50	2.1827873E-11	45.0	15
AGGCGAT	40	6.7739165E-9	45.0	7
ACGGGTC	20	7.0212013E-4	45.0	5
TTGACGG	25	3.8807906E-5	45.0	2
GCGATAT	20	7.0212013E-4	45.0	9
CGTACGG	20	7.0212013E-4	45.0	31
TGCAACG	20	7.0212013E-4	45.0	1
TACGGCT	195	0.0	42.692307	7
CGTTTTT	965	0.0	41.96891	1
>>END_MODULE