##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936350.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	153222
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.019527221939406	33.0	31.0	34.0	30.0	34.0
2	32.141239508686745	33.0	31.0	34.0	30.0	34.0
3	32.043766560937726	33.0	31.0	34.0	30.0	34.0
4	35.78633616582475	37.0	35.0	37.0	35.0	37.0
5	35.86345955541633	37.0	35.0	37.0	35.0	37.0
6	35.840636462126845	37.0	35.0	37.0	35.0	37.0
7	26.821154925532888	35.0	10.0	37.0	0.0	37.0
8	31.153600657868974	35.0	19.0	37.0	17.0	37.0
9	35.93758076516427	39.0	32.0	39.0	32.0	39.0
10	36.654527417733746	38.0	35.0	39.0	33.0	39.0
11	37.049483755596455	39.0	37.0	39.0	34.0	39.0
12	37.10608789860464	39.0	37.0	39.0	34.0	39.0
13	37.06234091710068	39.0	37.0	39.0	33.0	39.0
14	38.23296915586535	40.0	37.0	41.0	33.0	41.0
15	38.32147472295101	40.0	37.0	41.0	34.0	41.0
16	38.268864784430434	40.0	37.0	41.0	34.0	41.0
17	38.17251439088382	40.0	37.0	41.0	34.0	41.0
18	38.05563169779796	40.0	37.0	41.0	33.0	41.0
19	37.94560180652909	40.0	37.0	41.0	33.0	41.0
20	38.01326832961324	40.0	37.0	41.0	34.0	41.0
21	37.962009372022294	40.0	37.0	41.0	33.0	41.0
22	37.96856848233283	40.0	37.0	41.0	33.0	41.0
23	37.921434258787905	40.0	36.0	41.0	33.0	41.0
24	37.857344245604416	40.0	36.0	41.0	33.0	41.0
25	37.75111276448552	40.0	36.0	41.0	33.0	41.0
26	37.63466081894245	40.0	36.0	41.0	33.0	41.0
27	37.51488036965971	40.0	36.0	41.0	33.0	41.0
28	37.425049927556095	40.0	36.0	41.0	33.0	41.0
29	37.41874534988448	40.0	36.0	41.0	33.0	41.0
30	37.342326819908365	39.0	36.0	41.0	32.0	41.0
31	37.15169492631606	39.0	35.0	41.0	32.0	41.0
32	37.03451201524585	39.0	35.0	41.0	31.0	41.0
33	36.94100063959483	39.0	35.0	41.0	31.0	41.0
34	36.77461461147877	39.0	35.0	41.0	31.0	41.0
35	36.7214760282466	39.0	35.0	41.0	30.0	41.0
36	36.45676861025179	39.0	35.0	41.0	30.0	41.0
37	36.346177441881714	39.0	35.0	41.0	30.0	41.0
38	36.17346073018235	39.0	35.0	41.0	29.0	41.0
39	36.131932751171504	39.0	35.0	41.0	29.0	41.0
40	35.94312827139706	39.0	35.0	40.0	28.0	41.0
41	35.781212880656824	39.0	35.0	40.0	27.0	41.0
42	35.68473848402971	38.0	35.0	40.0	27.0	41.0
43	35.584661471590245	38.0	35.0	40.0	27.0	41.0
44	35.34075393872943	38.0	34.0	40.0	26.0	41.0
45	35.25249637780475	38.0	34.0	40.0	25.0	41.0
46	35.01134301862657	38.0	34.0	40.0	24.0	41.0
47	34.84706504287896	38.0	34.0	40.0	23.0	41.0
48	34.79744423124617	38.0	34.0	40.0	23.0	41.0
49	34.62455783112086	38.0	34.0	40.0	23.0	41.0
50	34.39929644568012	37.0	33.0	40.0	22.0	41.0
51	32.19341869966454	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	11.0
10	11.0
11	10.0
12	6.0
13	7.0
14	8.0
15	9.0
16	26.0
17	29.0
18	65.0
19	102.0
20	193.0
21	294.0
22	454.0
23	712.0
24	1023.0
25	1354.0
26	1683.0
27	2037.0
28	2294.0
29	2599.0
30	3118.0
31	4010.0
32	5038.0
33	6954.0
34	10550.0
35	13231.0
36	14249.0
37	22823.0
38	28977.0
39	31334.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05097179256243	19.733458641709415	23.521426427014397	11.69414313871376
2	33.247183824777125	25.166098863087544	24.889376199240317	16.697341112895014
3	33.871767761809664	23.852318857605304	25.934917962172534	16.3409954184125
4	28.617953035464883	26.306274555873177	25.280964874495833	19.79480753416611
5	25.67516414091971	29.76596050175562	24.49060839827179	20.068266959052877
6	25.878137604260488	35.085692655101745	24.249128715197557	14.787041025440212
7	60.59573690462206	28.35819921421206	6.722924906345042	4.323138974820848
8	80.91592591142263	6.658312774927882	8.12938089830442	4.296380415345055
9	73.95935309550848	9.05157222853115	10.47891294983749	6.51016172612288
10	41.315215830624844	26.417877328320998	17.790526164649986	14.47638067640417
11	31.97191003902834	27.033324196264246	23.140280116432365	17.854485648275052
12	30.96683243920586	22.762397044810797	27.43927112294579	18.831499393037554
13	26.220125047316966	24.251086658573833	28.566393859889573	20.96239443421963
14	21.19538969599666	28.25051232851679	28.656459255198342	21.89763872028821
15	20.39459085509914	27.121431648196737	33.27655297542129	19.207424521282846
16	23.37197008262521	25.007505449609063	31.56596311234679	20.054561355418933
17	23.819033820208585	24.725561603425096	28.22571171241728	23.22969286394904
18	23.649345394264532	26.790539217605826	28.946234874887416	20.613880513242226
19	25.53484486561982	27.402722846588613	26.90997376355876	20.152458524232813
20	26.9935126809466	26.156165563691896	27.875239848063593	18.97508190729791
21	26.02628865306549	26.61497696153294	27.837386276122228	19.521348109279344
22	23.36479095691219	25.906854107112554	27.50779914111551	23.220555794859745
23	23.81707587683231	26.62019814386968	28.018169714531854	21.544556264766157
24	22.999308193340383	24.993147198183028	29.45856339168005	22.548981216796545
25	22.4308519664278	26.774875670595605	28.354935975251593	22.439336387725
26	21.656159037213975	28.433253710302697	27.670308441346542	22.240278811136783
27	22.648183681194606	28.388221012648312	28.606858022999308	20.356737283157774
28	20.876244925663418	27.38444870841002	30.774301340538564	20.965005025388
29	22.333607445406013	26.699168526712874	29.453342209343308	21.513881818537808
30	23.630418608293848	26.679589092950103	28.136951612692695	21.55304068606336
31	24.251086658573833	27.053556277819112	26.792497160982105	21.90285990262495
32	24.788868439258067	27.637676051741916	27.70555142211954	19.867904086880475
33	23.906488624348984	26.814034538121156	28.041012387255094	21.238464450274765
34	23.45681429559724	26.08502695435381	29.219694299774186	21.238464450274765
35	23.8268655937137	24.866533526517077	28.305987390844656	23.000613488924568
36	24.080092937045595	27.31004686011147	27.473861455926695	21.13599874691624
37	23.862108574486694	26.90866846797457	29.35283444936106	19.876388508177676
38	22.860294213624673	26.443330592212604	28.50112908068032	22.195246113482398
39	23.28647322186109	25.392567646943647	29.147250394851916	22.173708736343343
40	23.96783751680568	25.13934030361175	30.40751328138257	20.485308898199996
41	21.83890041899988	25.2927125347535	29.305843808330394	23.562543237916227
42	22.761744397018706	25.73390244220804	28.77589380115127	22.728459359621986
43	23.475088433775827	25.34231376695253	28.510918797561708	22.671679001709936
44	22.336218036574383	26.180966179791415	28.37386276122228	23.108953022411924
45	23.020192922687343	25.671900901959248	27.370090456983988	23.937815718369425
46	22.5528971035491	27.422302280351385	28.29815561733955	21.72664499875997
47	22.49676939342914	25.196773309315894	29.937606871075957	22.36885042617901
48	21.972040568586756	25.027084883371842	29.932385688739217	23.06848885930219
49	21.39509992037697	24.73469867251439	30.658782681338188	23.21141872577045
50	20.555142211953896	25.873569069715835	29.25558992833927	24.315698789990993
51	21.083134275756745	25.542023991332837	28.56052002976074	24.814321703149677
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	103.0
1	240.0
2	377.0
3	510.0
4	643.0
5	478.0
6	313.0
7	308.5
8	304.0
9	303.5
10	303.0
11	303.0
12	303.0
13	317.5
14	332.0
15	362.5
16	393.0
17	408.5
18	424.0
19	455.0
20	486.0
21	566.0
22	646.0
23	726.0
24	806.0
25	1014.0
26	1413.0
27	1604.0
28	1911.5
29	2219.0
30	2586.5
31	2954.0
32	3321.0
33	3688.0
34	4251.0
35	4814.0
36	5108.5
37	5403.0
38	5852.0
39	6301.0
40	7044.5
41	7788.0
42	8612.0
43	9436.0
44	10193.0
45	10950.0
46	11518.5
47	12087.0
48	12481.0
49	12875.0
50	12553.0
51	12231.0
52	11224.5
53	10218.0
54	9110.5
55	8003.0
56	7802.5
57	7602.0
58	7396.0
59	7190.0
60	6834.5
61	6479.0
62	5673.0
63	4867.0
64	4091.5
65	3316.0
66	2761.0
67	2206.0
68	1916.0
69	1626.0
70	1354.0
71	1082.0
72	882.0
73	682.0
74	532.5
75	297.5
76	212.0
77	191.0
78	170.0
79	129.5
80	89.0
81	63.5
82	38.0
83	34.0
84	30.0
85	19.0
86	8.0
87	6.5
88	5.0
89	3.5
90	2.0
91	3.0
92	4.0
93	3.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	153222.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.69223740716085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.56312008210742	33.65313075145867
2	9.843311666096476	9.388991137042984
3	5.238453643516935	7.495007244390492
4	3.5620937393089287	6.795368811267312
5	2.671228190215532	6.369842450822989
6	1.8227848101265824	5.215961154403415
7	1.464249059185768	4.88833196277297
8	1.1823469038658914	4.511101538943494
9	0.8963393773520355	3.8473587343854017
>10	2.738282586383852	16.907493701948805
>50	0.009579199452617175	0.28455443735233843
>100	0.008210742387957578	0.6428580752111316
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	251	0.16381459581522234	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	174	0.11356071582409838	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	172	0.11225542023991332	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08876009972458263	0.0
2	0.0	0.0	0.0	0.4986229131586848	0.0
3	0.0	0.0	0.0	0.625889232616726	0.0
4	0.0	0.0	0.0	0.8021041364817063	0.0
5	0.0	0.0	0.0	1.3738236023547532	0.0
6	0.0	0.0	0.0	1.6681677565884794	0.0
7	0.0	0.0	0.0	1.9696910365352234	0.0
8	0.0	0.0	0.0	2.5192204774771247	0.0
9	0.0	0.0	0.0	2.728720418738823	0.0
10	0.0	0.0	0.0	3.567372831577711	0.0
11	0.0	0.0	0.0	4.537207450627195	0.0
12	0.0	0.0	0.0	5.5566433018757095	0.0
13	0.0	0.0	0.0	5.882314550129877	0.0
14	0.0	0.0	0.0	6.036339429063712	0.0
15	0.0	0.0	0.0	6.3306835832974375	0.0
16	0.0	0.0	0.0	6.950046337993238	0.0
17	0.0	0.0	0.0	7.592251765412278	0.0
18	6.526477920925193E-4	0.0	0.0	8.291237550743366	0.0
19	6.526477920925193E-4	0.0	0.0	8.672383861325397	0.0
20	6.526477920925193E-4	0.0	0.0	9.101173460730182	0.0
21	6.526477920925193E-4	0.0	0.0	9.590659304799573	0.0
22	0.0013052955841850386	0.0	0.0	10.108861651721032	0.0
23	0.0013052955841850386	0.0	0.0	10.62119016851366	0.0
24	0.0013052955841850386	0.0	0.0	11.012126195977078	0.0
25	0.0013052955841850386	0.0	0.0	11.361292764746576	0.0
26	0.0013052955841850386	0.0	0.0	11.692185195337483	0.0
27	0.0013052955841850386	0.0	0.0	12.03286734280978	0.0
28	0.0013052955841850386	0.0	0.0	12.373549490282075	0.0
29	0.0013052955841850386	0.0	0.0	12.725326650219943	0.0
30	0.0013052955841850386	0.0	0.0	13.132578872485674	0.0
31	0.0013052955841850386	0.0	0.0	13.526778138909556	0.0
32	0.0013052955841850386	0.0	0.0	13.920977405333439	0.0
33	0.0013052955841850386	0.0	0.0	14.296249885786636	0.0
34	0.0013052955841850386	0.0	0.0	14.643458511179857	0.0
35	0.0013052955841850386	0.0	0.0	15.038310425395832	0.0
36	0.0013052955841850386	0.0	0.0	15.427288509482972	0.0
37	0.0013052955841850386	0.0	0.0	15.835193379540797	0.0
38	0.0013052955841850386	0.0	0.0	16.201981438696794	0.0
39	0.0013052955841850386	0.0	0.0	16.609233660962524	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	25	3.87494E-5	45.000004	4
TACGGCT	25	3.87494E-5	45.000004	7
TCGATCA	25	3.87494E-5	45.000004	17
CTCGATC	25	3.87494E-5	45.000004	16
ACAAGCG	25	3.87494E-5	45.000004	14
ATACTCG	20	7.014137E-4	45.0	44
TTAAGTC	20	7.014137E-4	45.0	41
TACTCGC	20	7.014137E-4	45.0	45
CAAGCGA	20	7.014137E-4	45.0	15
GTCTTGC	20	7.014137E-4	45.0	45
ATTTCCG	65	0.0	41.53846	32
TAGTTGG	45	1.909575E-8	40.0	2
CGGGAAT	45	1.909575E-8	40.0	6
GACGAGA	40	3.4321238E-7	39.375	13
CTCGTAA	35	6.2129075E-6	38.57143	33
CGGTTCG	35	6.2129075E-6	38.57143	29
TTTCCGC	70	0.0	38.57143	33
TCGTAAC	35	6.2129075E-6	38.57143	34
CGTAACC	35	6.2129075E-6	38.57143	35
GTAAGGC	35	6.2129075E-6	38.57143	4
>>END_MODULE