##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936350.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 153222 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.019527221939406 33.0 31.0 34.0 30.0 34.0 2 32.141239508686745 33.0 31.0 34.0 30.0 34.0 3 32.043766560937726 33.0 31.0 34.0 30.0 34.0 4 35.78633616582475 37.0 35.0 37.0 35.0 37.0 5 35.86345955541633 37.0 35.0 37.0 35.0 37.0 6 35.840636462126845 37.0 35.0 37.0 35.0 37.0 7 26.821154925532888 35.0 10.0 37.0 0.0 37.0 8 31.153600657868974 35.0 19.0 37.0 17.0 37.0 9 35.93758076516427 39.0 32.0 39.0 32.0 39.0 10 36.654527417733746 38.0 35.0 39.0 33.0 39.0 11 37.049483755596455 39.0 37.0 39.0 34.0 39.0 12 37.10608789860464 39.0 37.0 39.0 34.0 39.0 13 37.06234091710068 39.0 37.0 39.0 33.0 39.0 14 38.23296915586535 40.0 37.0 41.0 33.0 41.0 15 38.32147472295101 40.0 37.0 41.0 34.0 41.0 16 38.268864784430434 40.0 37.0 41.0 34.0 41.0 17 38.17251439088382 40.0 37.0 41.0 34.0 41.0 18 38.05563169779796 40.0 37.0 41.0 33.0 41.0 19 37.94560180652909 40.0 37.0 41.0 33.0 41.0 20 38.01326832961324 40.0 37.0 41.0 34.0 41.0 21 37.962009372022294 40.0 37.0 41.0 33.0 41.0 22 37.96856848233283 40.0 37.0 41.0 33.0 41.0 23 37.921434258787905 40.0 36.0 41.0 33.0 41.0 24 37.857344245604416 40.0 36.0 41.0 33.0 41.0 25 37.75111276448552 40.0 36.0 41.0 33.0 41.0 26 37.63466081894245 40.0 36.0 41.0 33.0 41.0 27 37.51488036965971 40.0 36.0 41.0 33.0 41.0 28 37.425049927556095 40.0 36.0 41.0 33.0 41.0 29 37.41874534988448 40.0 36.0 41.0 33.0 41.0 30 37.342326819908365 39.0 36.0 41.0 32.0 41.0 31 37.15169492631606 39.0 35.0 41.0 32.0 41.0 32 37.03451201524585 39.0 35.0 41.0 31.0 41.0 33 36.94100063959483 39.0 35.0 41.0 31.0 41.0 34 36.77461461147877 39.0 35.0 41.0 31.0 41.0 35 36.7214760282466 39.0 35.0 41.0 30.0 41.0 36 36.45676861025179 39.0 35.0 41.0 30.0 41.0 37 36.346177441881714 39.0 35.0 41.0 30.0 41.0 38 36.17346073018235 39.0 35.0 41.0 29.0 41.0 39 36.131932751171504 39.0 35.0 41.0 29.0 41.0 40 35.94312827139706 39.0 35.0 40.0 28.0 41.0 41 35.781212880656824 39.0 35.0 40.0 27.0 41.0 42 35.68473848402971 38.0 35.0 40.0 27.0 41.0 43 35.584661471590245 38.0 35.0 40.0 27.0 41.0 44 35.34075393872943 38.0 34.0 40.0 26.0 41.0 45 35.25249637780475 38.0 34.0 40.0 25.0 41.0 46 35.01134301862657 38.0 34.0 40.0 24.0 41.0 47 34.84706504287896 38.0 34.0 40.0 23.0 41.0 48 34.79744423124617 38.0 34.0 40.0 23.0 41.0 49 34.62455783112086 38.0 34.0 40.0 23.0 41.0 50 34.39929644568012 37.0 33.0 40.0 22.0 41.0 51 32.19341869966454 35.0 30.0 39.0 15.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 11.0 10 11.0 11 10.0 12 6.0 13 7.0 14 8.0 15 9.0 16 26.0 17 29.0 18 65.0 19 102.0 20 193.0 21 294.0 22 454.0 23 712.0 24 1023.0 25 1354.0 26 1683.0 27 2037.0 28 2294.0 29 2599.0 30 3118.0 31 4010.0 32 5038.0 33 6954.0 34 10550.0 35 13231.0 36 14249.0 37 22823.0 38 28977.0 39 31334.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.05097179256243 19.733458641709415 23.521426427014397 11.69414313871376 2 33.247183824777125 25.166098863087544 24.889376199240317 16.697341112895014 3 33.871767761809664 23.852318857605304 25.934917962172534 16.3409954184125 4 28.617953035464883 26.306274555873177 25.280964874495833 19.79480753416611 5 25.67516414091971 29.76596050175562 24.49060839827179 20.068266959052877 6 25.878137604260488 35.085692655101745 24.249128715197557 14.787041025440212 7 60.59573690462206 28.35819921421206 6.722924906345042 4.323138974820848 8 80.91592591142263 6.658312774927882 8.12938089830442 4.296380415345055 9 73.95935309550848 9.05157222853115 10.47891294983749 6.51016172612288 10 41.315215830624844 26.417877328320998 17.790526164649986 14.47638067640417 11 31.97191003902834 27.033324196264246 23.140280116432365 17.854485648275052 12 30.96683243920586 22.762397044810797 27.43927112294579 18.831499393037554 13 26.220125047316966 24.251086658573833 28.566393859889573 20.96239443421963 14 21.19538969599666 28.25051232851679 28.656459255198342 21.89763872028821 15 20.39459085509914 27.121431648196737 33.27655297542129 19.207424521282846 16 23.37197008262521 25.007505449609063 31.56596311234679 20.054561355418933 17 23.819033820208585 24.725561603425096 28.22571171241728 23.22969286394904 18 23.649345394264532 26.790539217605826 28.946234874887416 20.613880513242226 19 25.53484486561982 27.402722846588613 26.90997376355876 20.152458524232813 20 26.9935126809466 26.156165563691896 27.875239848063593 18.97508190729791 21 26.02628865306549 26.61497696153294 27.837386276122228 19.521348109279344 22 23.36479095691219 25.906854107112554 27.50779914111551 23.220555794859745 23 23.81707587683231 26.62019814386968 28.018169714531854 21.544556264766157 24 22.999308193340383 24.993147198183028 29.45856339168005 22.548981216796545 25 22.4308519664278 26.774875670595605 28.354935975251593 22.439336387725 26 21.656159037213975 28.433253710302697 27.670308441346542 22.240278811136783 27 22.648183681194606 28.388221012648312 28.606858022999308 20.356737283157774 28 20.876244925663418 27.38444870841002 30.774301340538564 20.965005025388 29 22.333607445406013 26.699168526712874 29.453342209343308 21.513881818537808 30 23.630418608293848 26.679589092950103 28.136951612692695 21.55304068606336 31 24.251086658573833 27.053556277819112 26.792497160982105 21.90285990262495 32 24.788868439258067 27.637676051741916 27.70555142211954 19.867904086880475 33 23.906488624348984 26.814034538121156 28.041012387255094 21.238464450274765 34 23.45681429559724 26.08502695435381 29.219694299774186 21.238464450274765 35 23.8268655937137 24.866533526517077 28.305987390844656 23.000613488924568 36 24.080092937045595 27.31004686011147 27.473861455926695 21.13599874691624 37 23.862108574486694 26.90866846797457 29.35283444936106 19.876388508177676 38 22.860294213624673 26.443330592212604 28.50112908068032 22.195246113482398 39 23.28647322186109 25.392567646943647 29.147250394851916 22.173708736343343 40 23.96783751680568 25.13934030361175 30.40751328138257 20.485308898199996 41 21.83890041899988 25.2927125347535 29.305843808330394 23.562543237916227 42 22.761744397018706 25.73390244220804 28.77589380115127 22.728459359621986 43 23.475088433775827 25.34231376695253 28.510918797561708 22.671679001709936 44 22.336218036574383 26.180966179791415 28.37386276122228 23.108953022411924 45 23.020192922687343 25.671900901959248 27.370090456983988 23.937815718369425 46 22.5528971035491 27.422302280351385 28.29815561733955 21.72664499875997 47 22.49676939342914 25.196773309315894 29.937606871075957 22.36885042617901 48 21.972040568586756 25.027084883371842 29.932385688739217 23.06848885930219 49 21.39509992037697 24.73469867251439 30.658782681338188 23.21141872577045 50 20.555142211953896 25.873569069715835 29.25558992833927 24.315698789990993 51 21.083134275756745 25.542023991332837 28.56052002976074 24.814321703149677 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 103.0 1 240.0 2 377.0 3 510.0 4 643.0 5 478.0 6 313.0 7 308.5 8 304.0 9 303.5 10 303.0 11 303.0 12 303.0 13 317.5 14 332.0 15 362.5 16 393.0 17 408.5 18 424.0 19 455.0 20 486.0 21 566.0 22 646.0 23 726.0 24 806.0 25 1014.0 26 1413.0 27 1604.0 28 1911.5 29 2219.0 30 2586.5 31 2954.0 32 3321.0 33 3688.0 34 4251.0 35 4814.0 36 5108.5 37 5403.0 38 5852.0 39 6301.0 40 7044.5 41 7788.0 42 8612.0 43 9436.0 44 10193.0 45 10950.0 46 11518.5 47 12087.0 48 12481.0 49 12875.0 50 12553.0 51 12231.0 52 11224.5 53 10218.0 54 9110.5 55 8003.0 56 7802.5 57 7602.0 58 7396.0 59 7190.0 60 6834.5 61 6479.0 62 5673.0 63 4867.0 64 4091.5 65 3316.0 66 2761.0 67 2206.0 68 1916.0 69 1626.0 70 1354.0 71 1082.0 72 882.0 73 682.0 74 532.5 75 297.5 76 212.0 77 191.0 78 170.0 79 129.5 80 89.0 81 63.5 82 38.0 83 34.0 84 30.0 85 19.0 86 8.0 87 6.5 88 5.0 89 3.5 90 2.0 91 3.0 92 4.0 93 3.5 94 3.0 95 2.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 153222.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.69223740716085 #Duplication Level Percentage of deduplicated Percentage of total 1 70.56312008210742 33.65313075145867 2 9.843311666096476 9.388991137042984 3 5.238453643516935 7.495007244390492 4 3.5620937393089287 6.795368811267312 5 2.671228190215532 6.369842450822989 6 1.8227848101265824 5.215961154403415 7 1.464249059185768 4.88833196277297 8 1.1823469038658914 4.511101538943494 9 0.8963393773520355 3.8473587343854017 >10 2.738282586383852 16.907493701948805 >50 0.009579199452617175 0.28455443735233843 >100 0.008210742387957578 0.6428580752111316 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 251 0.16381459581522234 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG 174 0.11356071582409838 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC 172 0.11225542023991332 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.08876009972458263 0.0 2 0.0 0.0 0.0 0.4986229131586848 0.0 3 0.0 0.0 0.0 0.625889232616726 0.0 4 0.0 0.0 0.0 0.8021041364817063 0.0 5 0.0 0.0 0.0 1.3738236023547532 0.0 6 0.0 0.0 0.0 1.6681677565884794 0.0 7 0.0 0.0 0.0 1.9696910365352234 0.0 8 0.0 0.0 0.0 2.5192204774771247 0.0 9 0.0 0.0 0.0 2.728720418738823 0.0 10 0.0 0.0 0.0 3.567372831577711 0.0 11 0.0 0.0 0.0 4.537207450627195 0.0 12 0.0 0.0 0.0 5.5566433018757095 0.0 13 0.0 0.0 0.0 5.882314550129877 0.0 14 0.0 0.0 0.0 6.036339429063712 0.0 15 0.0 0.0 0.0 6.3306835832974375 0.0 16 0.0 0.0 0.0 6.950046337993238 0.0 17 0.0 0.0 0.0 7.592251765412278 0.0 18 6.526477920925193E-4 0.0 0.0 8.291237550743366 0.0 19 6.526477920925193E-4 0.0 0.0 8.672383861325397 0.0 20 6.526477920925193E-4 0.0 0.0 9.101173460730182 0.0 21 6.526477920925193E-4 0.0 0.0 9.590659304799573 0.0 22 0.0013052955841850386 0.0 0.0 10.108861651721032 0.0 23 0.0013052955841850386 0.0 0.0 10.62119016851366 0.0 24 0.0013052955841850386 0.0 0.0 11.012126195977078 0.0 25 0.0013052955841850386 0.0 0.0 11.361292764746576 0.0 26 0.0013052955841850386 0.0 0.0 11.692185195337483 0.0 27 0.0013052955841850386 0.0 0.0 12.03286734280978 0.0 28 0.0013052955841850386 0.0 0.0 12.373549490282075 0.0 29 0.0013052955841850386 0.0 0.0 12.725326650219943 0.0 30 0.0013052955841850386 0.0 0.0 13.132578872485674 0.0 31 0.0013052955841850386 0.0 0.0 13.526778138909556 0.0 32 0.0013052955841850386 0.0 0.0 13.920977405333439 0.0 33 0.0013052955841850386 0.0 0.0 14.296249885786636 0.0 34 0.0013052955841850386 0.0 0.0 14.643458511179857 0.0 35 0.0013052955841850386 0.0 0.0 15.038310425395832 0.0 36 0.0013052955841850386 0.0 0.0 15.427288509482972 0.0 37 0.0013052955841850386 0.0 0.0 15.835193379540797 0.0 38 0.0013052955841850386 0.0 0.0 16.201981438696794 0.0 39 0.0013052955841850386 0.0 0.0 16.609233660962524 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGGT 25 3.87494E-5 45.000004 4 TACGGCT 25 3.87494E-5 45.000004 7 TCGATCA 25 3.87494E-5 45.000004 17 CTCGATC 25 3.87494E-5 45.000004 16 ACAAGCG 25 3.87494E-5 45.000004 14 ATACTCG 20 7.014137E-4 45.0 44 TTAAGTC 20 7.014137E-4 45.0 41 TACTCGC 20 7.014137E-4 45.0 45 CAAGCGA 20 7.014137E-4 45.0 15 GTCTTGC 20 7.014137E-4 45.0 45 ATTTCCG 65 0.0 41.53846 32 TAGTTGG 45 1.909575E-8 40.0 2 CGGGAAT 45 1.909575E-8 40.0 6 GACGAGA 40 3.4321238E-7 39.375 13 CTCGTAA 35 6.2129075E-6 38.57143 33 CGGTTCG 35 6.2129075E-6 38.57143 29 TTTCCGC 70 0.0 38.57143 33 TCGTAAC 35 6.2129075E-6 38.57143 34 CGTAACC 35 6.2129075E-6 38.57143 35 GTAAGGC 35 6.2129075E-6 38.57143 4 >>END_MODULE