##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936346.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	170271
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.816709833148334	31.0	31.0	34.0	30.0	34.0
2	31.92394477039543	33.0	31.0	34.0	30.0	34.0
3	31.73161607085176	33.0	31.0	34.0	30.0	34.0
4	35.56186902056134	37.0	35.0	37.0	33.0	37.0
5	35.665415719646916	37.0	35.0	37.0	35.0	37.0
6	35.64198835973243	37.0	35.0	37.0	33.0	37.0
7	26.93782264742675	35.0	17.0	37.0	0.0	37.0
8	31.121212655120367	35.0	23.0	37.0	17.0	37.0
9	35.791362005273946	38.0	32.0	39.0	32.0	39.0
10	36.52650187054754	37.0	35.0	39.0	32.0	39.0
11	36.87347816128407	39.0	37.0	39.0	33.0	39.0
12	36.944688173558625	39.0	37.0	39.0	33.0	39.0
13	36.90606738669533	39.0	37.0	39.0	33.0	39.0
14	38.09791450100135	40.0	37.0	41.0	33.0	41.0
15	38.12292756840566	40.0	37.0	41.0	33.0	41.0
16	38.092699285257034	40.0	37.0	41.0	33.0	41.0
17	37.93409917132101	40.0	37.0	41.0	33.0	41.0
18	37.8064497183901	40.0	36.0	41.0	33.0	41.0
19	37.63969789335823	39.0	36.0	41.0	32.0	41.0
20	37.72529673285527	39.0	36.0	41.0	33.0	41.0
21	37.72431594340786	39.0	36.0	41.0	33.0	41.0
22	37.73013020420389	39.0	36.0	41.0	33.0	41.0
23	37.71120155516794	39.0	36.0	41.0	33.0	41.0
24	37.64778500155634	39.0	36.0	41.0	33.0	41.0
25	37.553670325539876	39.0	36.0	41.0	33.0	41.0
26	37.42756546916386	39.0	35.0	41.0	33.0	41.0
27	37.301531088676285	39.0	35.0	41.0	32.0	41.0
28	37.27198994543992	39.0	35.0	41.0	32.0	41.0
29	37.228670766014176	39.0	35.0	41.0	32.0	41.0
30	37.08825343129482	39.0	35.0	41.0	32.0	41.0
31	36.96246571641677	39.0	35.0	41.0	31.0	41.0
32	36.842985593553806	39.0	35.0	41.0	31.0	41.0
33	36.69772891449513	39.0	35.0	41.0	31.0	41.0
34	36.57853656817661	39.0	35.0	41.0	30.0	41.0
35	36.33266968538389	39.0	35.0	41.0	30.0	41.0
36	36.156750121864555	39.0	35.0	40.0	29.0	41.0
37	36.101943372623644	39.0	35.0	40.0	29.0	41.0
38	35.99800318316096	39.0	35.0	40.0	29.0	41.0
39	36.00756441202554	39.0	35.0	40.0	29.0	41.0
40	35.76531529150589	38.0	35.0	40.0	28.0	41.0
41	35.703507937346934	38.0	35.0	40.0	27.0	41.0
42	35.63195141862091	38.0	35.0	40.0	27.0	41.0
43	35.61259991425434	38.0	35.0	40.0	27.0	41.0
44	35.408219837787996	38.0	34.0	40.0	26.0	41.0
45	35.32333750315673	38.0	34.0	40.0	26.0	41.0
46	35.084435987337834	38.0	34.0	40.0	25.0	41.0
47	34.88735016532469	38.0	34.0	40.0	24.0	41.0
48	34.773778271108995	38.0	34.0	40.0	23.0	41.0
49	34.59496919616376	38.0	34.0	40.0	23.0	41.0
50	34.362304796471506	37.0	33.0	40.0	23.0	41.0
51	31.915217506210688	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	9.0
10	9.0
11	11.0
12	8.0
13	2.0
14	10.0
15	9.0
16	24.0
17	48.0
18	73.0
19	117.0
20	221.0
21	342.0
22	518.0
23	796.0
24	1117.0
25	1613.0
26	2059.0
27	2359.0
28	2778.0
29	3139.0
30	3821.0
31	4799.0
32	6245.0
33	8280.0
34	12184.0
35	14927.0
36	16492.0
37	25176.0
38	30662.0
39	32405.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.84464177693207	20.02924749370122	23.504296092699285	13.621814636667432
2	31.16032677320272	27.707595538876262	24.102166546270357	17.029911141650665
3	30.19715629790158	27.344057414357113	26.54885447316337	15.909931814577938
4	27.5425645001204	23.748025206876097	30.88077241573727	17.82863787726624
5	26.896535522784266	28.01240375636485	25.055940236446606	20.035120484404274
6	23.47962953174645	35.69662479224295	26.438442247945922	14.385303428064674
7	57.72738751754556	27.07801093550869	10.571970564570595	4.622630982375155
8	75.36162940254066	7.758808017806908	11.44410968397437	5.435452895678066
9	69.7687803560207	7.6918559237920725	12.550581132430066	9.988782587757164
10	38.90915070681443	24.702386196122653	20.97303709968227	15.415425997380646
11	29.034891437766852	24.812798421340098	26.887138737659377	19.26517140323367
12	28.03295922382555	21.45462233733284	30.605329151763954	19.90708928707766
13	23.440280494035978	23.838469263703153	33.3621109877783	19.35913925448256
14	19.494218040652843	27.689976566767093	29.711459966758873	23.10434542582119
15	17.848018746586327	26.062570842950354	37.037428569750574	19.051981840712745
16	20.768657023215933	26.265776321276086	30.679328834622453	22.28623782088553
17	20.76219673344257	25.65498528815829	30.335171579423392	23.24764639897575
18	21.138068138438136	26.740901269153287	30.503139113530782	21.617891478877787
19	22.07950854813797	29.046637419172967	27.405723816739197	21.46813021594987
20	23.896024572593102	27.597183313658814	29.37141380505195	19.13537830869614
21	22.5681413746322	29.177605111851108	28.509258769843367	19.74499474367332
22	20.57249913373387	25.22214587334308	28.65255974299793	25.55279524992512
23	21.035878100204965	28.143958748113302	29.44658808605106	21.37357506563067
24	22.74609299293479	23.488439017801035	29.68385691045451	24.08161107880966
25	20.63710203146748	29.389032777161113	27.076836337368075	22.897028854003324
26	20.816815546980987	26.925313177229242	28.25554557147136	24.002325704318412
27	21.576780543956396	29.0713039801258	27.477961602386785	21.87395387353102
28	18.50520640625826	26.8472024008786	31.796371666343653	22.85121952651949
29	23.375677596302367	25.20393960216361	28.532750732655593	22.887632068878435
30	21.165083895672193	29.75139630353965	28.583845751772174	20.49967404901598
31	21.363590981435475	26.819599344574236	29.397842263215697	22.41896741077459
32	24.009373293162074	27.73989698774307	28.77001955705904	19.480710162035813
33	21.219115410140304	28.657845434630673	26.85659918600349	23.266439969225527
34	22.070699062083385	25.2133363872885	31.758784525844096	20.95718002478402
35	22.81656888137146	26.578219426678647	28.192704570948663	22.412507121001227
36	22.24865068038597	28.047054401512884	28.308402487798862	21.395892430302283
37	20.36694445912692	28.165101514644302	32.09765608941041	19.370297936818364
38	20.422737870805953	28.448179666531587	28.32132306734558	22.807759395316875
39	21.7036371431424	25.556906343417257	30.565392814983174	22.174063698457168
40	24.13446799513716	25.52460489455045	29.963411267919966	20.37751584239242
41	19.672756958025737	25.968602991701463	29.313271197091694	25.045368853181106
42	21.392368635880448	24.915575758643573	30.5577579270692	23.134297678406774
43	22.38020567213442	25.29555825713128	27.92019780232688	24.404038268407422
44	21.21852811107	25.982110870318493	28.143958748113302	24.655402270498207
45	20.768657023215933	24.509752101062425	28.725972126786125	25.995618748935524
46	22.72671212361471	27.6059927997134	26.97288440192399	22.694410674747903
47	19.818994426531823	24.29303874411967	33.55474508283854	22.333221746509977
48	20.895513622401936	24.991337338712995	30.212426073729524	23.900722965155545
49	20.281786093932613	23.74097761803243	32.34314710079814	23.634089187236818
50	19.4689641806297	23.691057197056455	31.20907259603808	25.630906026275763
51	19.57761450863623	23.65112086027568	28.532163433585282	28.2391011975028
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	77.0
1	206.5
2	336.0
3	1123.0
4	1910.0
5	1339.5
6	769.0
7	641.0
8	513.0
9	519.0
10	525.0
11	547.5
12	570.0
13	559.0
14	548.0
15	550.0
16	552.0
17	544.5
18	537.0
19	540.0
20	543.0
21	598.5
22	654.0
23	737.5
24	821.0
25	1040.5
26	1530.5
27	1801.0
28	2006.0
29	2211.0
30	2535.5
31	2860.0
32	3355.0
33	3850.0
34	4418.0
35	4986.0
36	5330.0
37	5674.0
38	6446.0
39	7218.0
40	7981.0
41	8744.0
42	10190.5
43	11637.0
44	16504.0
45	21371.0
46	18746.5
47	16122.0
48	15201.0
49	14280.0
50	12325.5
51	10371.0
52	9220.5
53	8070.0
54	7694.5
55	7319.0
56	6995.5
57	6672.0
58	6612.0
59	6552.0
60	6170.5
61	5789.0
62	5455.5
63	5122.0
64	4295.0
65	3468.0
66	2864.0
67	2260.0
68	1910.0
69	1560.0
70	1345.5
71	1131.0
72	899.0
73	667.0
74	539.5
75	305.5
76	199.0
77	167.0
78	135.0
79	102.5
80	70.0
81	59.5
82	49.0
83	40.0
84	31.0
85	20.0
86	9.0
87	6.5
88	4.0
89	3.5
90	3.0
91	4.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	170271.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.369117465687054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.89039690312951	37.72163198665657
2	9.44335618674502	9.51307034080965
3	4.503054894827667	6.804447028560354
4	2.8776642880462666	5.797816422056604
5	2.0859568117158713	5.253390183883339
6	1.450492047945525	4.383600260760788
7	1.1286786996875144	3.9795385003905537
8	0.8570029382957884	3.4533185333967618
9	0.6949302737745441	3.1502722131190866
>10	2.015997388181521	13.299387447069671
>50	0.026817779021500864	1.0072179055740555
>100	0.020987827060305022	2.452560917596067
>500	0.001165990392239168	0.5820133786728215
>1k	0.003497971176717504	2.6017348814536825
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	1629	0.9567101855277763	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	1500	0.8809486054583575	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	1301	0.7640760904675488	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	991	0.5820133786728215	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	431	0.2531258993017014	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	412	0.24196721696589557	No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	399	0.2343323290519231	Illumina Single End Adapter 2 (95% over 21bp)
GAATCTATCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	339	0.1990943848335888	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	312	0.18323730993533838	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	274	0.16091994526372663	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC	266	0.15622155270128207	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	233	0.1368406833811982	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	226	0.1327295898890592	No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	213	0.12509470197508676	Illumina Single End Adapter 2 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTT	176	0.10336463637378061	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.872990703055717E-4	0.0	0.0	0.17266592666983807	0.0
2	5.872990703055717E-4	0.0	0.0	0.937916615277998	0.0
3	5.872990703055717E-4	0.0	0.0	1.2996928425862302	0.0
4	5.872990703055717E-4	0.0	0.0	1.7824526783774102	0.0
5	5.872990703055717E-4	0.0	0.0	3.74403157319802	0.0
6	5.872990703055717E-4	0.0	0.0	4.503996570173429	0.0
7	5.872990703055717E-4	0.0	0.0	5.38846897005362	0.0
8	5.872990703055717E-4	0.0	0.0	6.730447345701852	0.0
9	5.872990703055717E-4	0.0	0.0	7.183842227977753	0.0
10	5.872990703055717E-4	0.0	0.0	9.449054742146343	0.0
11	5.872990703055717E-4	0.0	0.0	10.779874435458769	0.0
12	5.872990703055717E-4	0.0	0.0	12.742040629349685	0.0
13	5.872990703055717E-4	0.0	0.0	13.213641782805057	0.0
14	5.872990703055717E-4	0.0	0.0	13.420958354622925	0.0
15	5.872990703055717E-4	0.0	0.0	13.850273975016298	0.0
16	5.872990703055717E-4	0.0	0.0	14.602016785007429	0.0
17	5.872990703055717E-4	0.0	0.0	15.418362492732173	0.0
18	5.872990703055717E-4	0.0	0.0	16.297549200979613	0.0
19	5.872990703055717E-4	0.0	0.0	16.820245373551575	0.0
20	5.872990703055717E-4	0.0	0.0	17.337068555420476	0.0
21	5.872990703055717E-4	0.0	0.0	17.98544672903783	0.0
22	5.872990703055717E-4	0.0	0.0	18.62971380916304	0.0
23	5.872990703055717E-4	0.0	0.0	19.32625050654545	0.0
24	5.872990703055717E-4	0.0	0.0	19.868914847507796	0.0
25	5.872990703055717E-4	0.0	0.0	20.37340474890028	0.0
26	5.872990703055717E-4	0.0	0.0	20.7862759953251	0.0
27	5.872990703055717E-4	0.0	0.0	21.224988400843362	0.0
28	5.872990703055717E-4	0.0	0.0	21.69071656359568	0.0
29	5.872990703055717E-4	0.0	0.0	22.208714343605195	0.0
30	5.872990703055717E-4	0.0	0.0	22.726124824544403	0.0
31	5.872990703055717E-4	0.0	0.0	23.223567137093223	0.0
32	5.872990703055717E-4	0.0	0.0	23.69986668311104	0.0
33	5.872990703055717E-4	0.0	0.0	24.1697059393555	0.0
34	5.872990703055717E-4	0.0	0.0	24.590799372764593	0.0
35	5.872990703055717E-4	0.0	0.0	25.077670302047913	0.0
36	5.872990703055717E-4	0.0	0.0	25.517557305706784	0.0
37	5.872990703055717E-4	0.0	0.0	26.01030122569316	0.0
38	5.872990703055717E-4	0.0	0.0	26.48131508007823	0.0
39	5.872990703055717E-4	0.0	0.0	26.963487616799103	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGG	25	3.8767706E-5	45.000004	3
CACATCC	25	3.8767706E-5	45.000004	45
AAACGGC	50	2.1827873E-11	45.000004	6
CGTTTTC	25	3.8767706E-5	45.000004	1
TCGGGAT	20	7.016348E-4	45.0	6
TGCGGGC	20	7.016348E-4	45.0	4
ATCGTTG	20	7.016348E-4	45.0	23
TGACCGG	20	7.016348E-4	45.0	2
AGTGACG	20	7.016348E-4	45.0	10
TACGAAT	45	3.8016879E-10	45.0	12
CGGCTTC	20	7.016348E-4	45.0	42
CCGGTCG	20	7.016348E-4	45.0	14
ATTATCC	20	7.016348E-4	45.0	45
GACGAGA	20	7.016348E-4	45.0	13
GGCGAAC	20	7.016348E-4	45.0	8
CTTAGGT	20	7.016348E-4	45.0	4
GACCTAT	40	6.7557266E-9	45.0	33
TGCATAT	65	0.0	44.999996	27
AGTTAAT	75	0.0	42.0	39
GCATATC	70	0.0	41.785717	28
>>END_MODULE