SRR2936344.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936344.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77210
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	1747	2.2626602771661704	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC	1686	2.1836549669731897	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1620	2.0981738116824244	RNA PCR Primer, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	450	0.5828260588006734	Illumina PCR Primer Index 7 (95% over 22bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC	412	0.5336096360575056	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT	410	0.5310192980183914	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	410	0.5310192980183914	No Hit
GAATCTATCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	350	0.45330915684496825	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTC	273	0.3535811423390752	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	228	0.2952985364590079	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTT	216	0.2797565082243233	RNA PCR Primer, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	168	0.21758839528558477	RNA PCR Primer, Index 16 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTA	167	0.2162932262660277	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163	0.2111125501877995	No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	162	0.20981738116824244	RNA PCR Primer, Index 16 (96% over 26bp)
GAATGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT	130	0.1683719725424168	RNA PCR Primer, Index 16 (95% over 21bp)
GAATGAATGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT	94	0.1217458878383629	No Hit
GAATCTATCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTT	88	0.1139748737210206	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTTC	87	0.11267970470146353	No Hit
GCTGTCACTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	86	0.1113845356819065	Illumina PCR Primer Index 7 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACT	40	6.673872E-9	45.0	3
CGTGGGG	40	6.673872E-9	45.0	4
CAACGCA	35	1.1923476E-7	45.0	17
ACGCAAG	35	1.1923476E-7	45.0	19
TTATGAC	35	1.1923476E-7	45.0	27
GGGTAAT	20	6.9924275E-4	45.0	8
AATGGGC	20	6.9924275E-4	45.0	4
ACTTATA	40	6.673872E-9	45.0	7
TGAAACC	50	2.1827873E-11	45.0	4
GACCCGC	25	3.856979E-5	45.0	31
ACGGGAC	25	3.856979E-5	45.0	5
AAACGGC	80	0.0	45.0	6
TCGTGGG	20	6.9924275E-4	45.0	3
TGACCCG	25	3.856979E-5	45.0	30
CTTATGA	35	1.1923476E-7	45.0	26
AACGCAA	35	1.1923476E-7	45.0	18
ACTAATC	20	6.9924275E-4	45.0	10
GACGGGA	20	6.9924275E-4	45.0	4
AGGGGTT	25	3.856979E-5	45.0	6
TGTAGGG	35	1.1923476E-7	45.0	3

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content