##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936344.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77210
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72257479601088	31.0	31.0	34.0	30.0	34.0
2	31.827936795751846	31.0	31.0	34.0	30.0	34.0
3	31.76705090014247	31.0	31.0	34.0	30.0	34.0
4	35.54592669343349	37.0	35.0	37.0	33.0	37.0
5	35.60277166170185	37.0	35.0	37.0	33.0	37.0
6	35.541030954539565	37.0	35.0	37.0	33.0	37.0
7	27.009869187929024	35.0	19.0	37.0	0.0	37.0
8	30.98570133402409	35.0	23.0	37.0	17.0	37.0
9	35.52902473772827	38.0	32.0	39.0	30.0	39.0
10	36.42271726460303	37.0	35.0	39.0	32.0	39.0
11	36.73445149592022	38.0	35.0	39.0	33.0	39.0
12	36.700297888874495	39.0	35.0	39.0	33.0	39.0
13	36.50450718818806	39.0	35.0	39.0	32.0	39.0
14	37.77710141173423	39.0	36.0	41.0	33.0	41.0
15	37.707732159046756	39.0	36.0	41.0	33.0	41.0
16	37.73671804170444	39.0	36.0	41.0	33.0	41.0
17	37.534671674653545	39.0	36.0	41.0	32.0	41.0
18	37.452415490221476	39.0	36.0	41.0	32.0	41.0
19	37.34713120062168	39.0	36.0	41.0	32.0	41.0
20	37.49664551223935	39.0	36.0	41.0	32.0	41.0
21	37.52506152052843	39.0	36.0	41.0	33.0	41.0
22	37.55328325346458	39.0	36.0	41.0	33.0	41.0
23	37.61973837585805	39.0	36.0	41.0	33.0	41.0
24	37.5216293226266	39.0	36.0	41.0	33.0	41.0
25	37.48305918922419	39.0	36.0	41.0	33.0	41.0
26	37.24685921512757	39.0	35.0	41.0	32.0	41.0
27	37.18558476881233	39.0	35.0	40.0	32.0	41.0
28	37.18593446444761	39.0	35.0	40.0	32.0	41.0
29	37.1289599792773	39.0	35.0	40.0	32.0	41.0
30	37.034671674653545	39.0	35.0	40.0	32.0	41.0
31	36.89077839658076	39.0	35.0	40.0	31.0	41.0
32	36.82721150110089	39.0	35.0	40.0	31.0	41.0
33	36.732651210983036	39.0	35.0	40.0	31.0	41.0
34	36.471260199456026	38.0	35.0	40.0	30.0	41.0
35	36.59040279756508	39.0	35.0	40.0	30.0	41.0
36	36.165431938868025	38.0	35.0	40.0	30.0	41.0
37	36.06496567802098	38.0	35.0	40.0	30.0	41.0
38	35.93811682424556	38.0	35.0	40.0	30.0	41.0
39	35.53797435565341	38.0	34.0	40.0	27.0	41.0
40	35.28283901049087	38.0	34.0	40.0	25.0	41.0
41	35.1298277425204	38.0	34.0	40.0	25.0	41.0
42	35.037883693822046	38.0	34.0	40.0	24.0	41.0
43	35.0853904934594	38.0	34.0	40.0	25.0	41.0
44	34.59162025644346	38.0	33.0	40.0	23.0	41.0
45	34.61678539049346	38.0	33.0	40.0	23.0	41.0
46	34.051210983033286	37.0	33.0	40.0	22.0	41.0
47	33.693498251521824	37.0	32.0	40.0	21.0	41.0
48	33.631097008159564	37.0	32.0	40.0	20.0	41.0
49	33.35688382333895	37.0	32.0	40.0	18.0	41.0
50	33.14821914259811	36.0	31.0	40.0	17.0	41.0
51	30.22157751586582	34.0	26.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	1.0
12	0.0
13	0.0
14	2.0
15	2.0
16	9.0
17	8.0
18	31.0
19	42.0
20	62.0
21	121.0
22	170.0
23	300.0
24	470.0
25	689.0
26	963.0
27	1217.0
28	1467.0
29	1862.0
30	2350.0
31	2786.0
32	3389.0
33	4323.0
34	6167.0
35	7777.0
36	7520.0
37	10521.0
38	12653.0
39	12297.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.86931744592669	18.500194275352932	21.08405646936925	11.546431809351121
2	26.01735526486206	34.39450848335708	22.333894573241807	17.25424167853905
3	27.853904934593963	34.18598627120839	23.666623494366014	14.293485299831628
4	26.429219013081205	21.052972412899884	35.70651470016838	16.811293873850538
5	30.055692267840957	24.788239865302423	22.756119673617405	22.399948193239215
6	22.11760134697578	37.52363683460692	26.702499676207747	13.656262142209558
7	53.81168242455641	26.60277166170185	14.341406553555238	5.244139360186504
8	66.98873202952986	12.200492164227432	13.649786297111774	7.160989509130941
9	62.57997668695765	6.536718041704442	13.442559253982644	17.440746017355266
10	40.52583862194016	21.21616370936407	22.45564046108017	15.802357207615595
11	29.518197124724775	22.87268488537754	27.176531537365626	20.432586452532057
12	26.926563916591117	19.408107758062425	32.039891205802356	21.6254371195441
13	18.904287009454734	25.876181841730343	37.83706773733972	17.382463411475197
14	19.309674912576092	30.47532703017744	27.842248413417952	22.37274964382852
15	15.295946120968788	24.030565988861547	43.75080948063722	16.922678409532445
16	16.4926822950395	26.451236886413675	30.181323662737984	26.874757155808833
17	17.228338298147907	23.604455381427275	32.69783706773734	26.469369252687475
18	17.38634891853387	26.636446056210332	33.468462634373786	22.508742390882013
19	18.581789923585028	27.909597202434917	28.144022794974745	25.36459007900531
20	20.32508742390882	27.90441652635669	33.16021240771921	18.610283642015283
21	20.323792254889263	30.6242714674265	30.084185986271205	18.96775029141303
22	17.39930060872944	23.73915295946121	29.923585027846133	28.937961403963218
23	17.171350861287397	29.825152182359798	30.121745887838365	22.88175106851444
24	22.91801580106204	21.927211501100892	28.416008289081724	26.738764408755344
25	17.541769200880715	32.67452402538532	26.917497733454216	22.866209040279756
26	17.480896256961532	26.829426240124338	28.605102965937057	27.084574536977073
27	22.524284419116693	28.909467685532963	25.779044165263564	22.787203730086777
28	15.071881880585417	23.974873721020593	36.05361999740966	24.89962440098433
29	20.77321590467556	25.795881362517807	27.379873073436084	26.05102965937055
30	22.41808055951302	27.076803522859734	29.453438673746923	21.051677243880327
31	22.29762984069421	26.278979406812592	27.67128610283642	23.75210464965678
32	23.38039114104391	26.83719725424168	22.822173293614817	26.960238311099598
33	19.257868151793808	22.480248672451754	23.710659241030953	34.55122393472348
34	18.919829037689418	29.975391788628414	26.530242196606658	24.57453697707551
35	19.1918145317964	23.372620126926563	23.550058282605878	33.88550705867115
36	19.71765315373656	26.457712731511464	28.296852739282475	25.527781375469498
37	20.452013987825413	24.325864525320554	30.085481155290765	25.13664033156327
38	16.297111773086385	24.38932780727885	26.49786297111773	32.815697448517035
39	20.3678280015542	26.203859603678282	27.110477917368215	26.3178344773993
40	19.79406812589043	22.476363165393085	28.375858049475454	29.353710659241035
41	17.49125760911799	28.510555627509387	24.19505245434529	29.803134309027328
42	18.1453179639943	21.077580624271466	31.994560290117864	28.782541121616372
43	23.37909597202435	21.460950654060355	24.86465483745629	30.295298536459008
44	19.82126667530113	22.305400854811552	29.51301644864655	28.360316021240774
45	18.34347882398653	22.31576220696801	26.830721409143894	32.51003755990157
46	26.105426758191946	25.692267840953242	24.56029011786038	23.64201528299443
47	16.33726201269266	21.67335837326771	36.59629581660407	25.393083797435565
48	21.209687864266286	23.034581012822173	26.44087553425722	29.31485558865432
49	17.9251392306696	18.934075896904545	36.514700168371974	26.62608470405388
50	19.89509130941588	20.10102318352545	31.845615852868796	28.158269654189873
51	18.439321331433753	20.463670509001425	26.242714674264995	34.854293485299834
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	41.0
1	82.0
2	123.0
3	232.5
4	342.0
5	259.0
6	176.0
7	155.5
8	135.0
9	147.0
10	159.0
11	151.0
12	143.0
13	145.0
14	147.0
15	152.0
16	157.0
17	152.0
18	147.0
19	184.5
20	222.0
21	229.0
22	236.0
23	311.0
24	386.0
25	429.5
26	528.5
27	584.0
28	751.5
29	919.0
30	1065.0
31	1211.0
32	1244.5
33	1278.0
34	1504.5
35	1731.0
36	1930.5
37	2130.0
38	2321.5
39	2513.0
40	2846.5
41	3180.0
42	3634.0
43	4088.0
44	4593.0
45	5098.0
46	5703.5
47	6309.0
48	10407.0
49	14505.0
50	11384.0
51	8263.0
52	7771.5
53	7280.0
54	5921.0
55	4562.0
56	3788.0
57	3014.0
58	2632.0
59	2250.0
60	1959.0
61	1668.0
62	1489.5
63	1311.0
64	1103.5
65	896.0
66	733.0
67	570.0
68	464.5
69	359.0
70	304.5
71	250.0
72	204.0
73	158.0
74	122.5
75	65.5
76	44.0
77	37.5
78	31.0
79	23.5
80	16.0
81	11.5
82	7.0
83	6.5
84	6.0
85	3.5
86	1.0
87	1.0
88	1.0
89	2.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	77210.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.803263825929285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.09089461532338	36.6468074083668
2	9.936042037100925	9.698225618443207
3	4.588519412966747	6.71804170444243
4	2.79716568031634	5.460432586452532
5	1.942623603407553	4.740318611578811
6	1.374698123722831	4.025385312783318
7	1.0854276691170617	3.7080689029918403
8	0.7775802128393621	3.0358761818417306
9	0.6024256256468777	2.6460303069550575
>10	1.7303150128712081	11.115140525838623
>50	0.03184628858045169	1.0775806242714674
>100	0.034500145962156	4.583603160212408
>500	0.0	0.0
>1k	0.007961572145112922	6.544489055821785
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	1747	2.2626602771661704	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC	1686	2.1836549669731897	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1620	2.0981738116824244	RNA PCR Primer, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	450	0.5828260588006734	Illumina PCR Primer Index 7 (95% over 22bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC	412	0.5336096360575056	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT	410	0.5310192980183914	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	410	0.5310192980183914	No Hit
GAATCTATCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	350	0.45330915684496825	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTC	273	0.3535811423390752	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	228	0.2952985364590079	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTT	216	0.2797565082243233	RNA PCR Primer, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	168	0.21758839528558477	RNA PCR Primer, Index 16 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTA	167	0.2162932262660277	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163	0.2111125501877995	No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	162	0.20981738116824244	RNA PCR Primer, Index 16 (96% over 26bp)
GAATGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT	130	0.1683719725424168	RNA PCR Primer, Index 16 (95% over 21bp)
GAATGAATGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT	94	0.1217458878383629	No Hit
GAATCTATCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTT	88	0.1139748737210206	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTTC	87	0.11267970470146353	No Hit
GCTGTCACTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	86	0.1113845356819065	Illumina PCR Primer Index 7 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3496956352804041	0.0
2	0.0	0.0	0.0	2.0968786426628676	0.0
3	0.0	0.0	0.0	2.605880067348789	0.0
4	0.0	0.0	0.0	3.4606916202564433	0.0
5	0.0	0.0	0.0	7.655744074601736	0.0
6	0.0	0.0	0.0	8.577904416526357	0.0
7	0.0	0.0	0.0	10.137287916073047	0.0
8	0.0	0.0	0.0	12.001036135215646	0.0
9	0.0	0.0	0.0	12.524284419116695	0.0
10	0.0	0.0	0.0	16.90713638129776	0.0
11	0.0	0.0	0.0	18.579199585545915	0.0
12	0.0	0.0	0.0	22.976298406942107	0.0
13	0.0	0.0	0.0	23.62517808574019	0.0
14	0.0	0.0	0.0	23.98134956611838	0.0
15	0.0	0.0	0.0	24.739023442559255	0.0
16	0.0	0.0	0.0	25.6844968268359	0.0
17	0.0	0.0	0.0	26.672710788757932	0.0
18	0.0	0.0	0.0	27.70237015930579	0.0
19	0.0	0.0	0.0	28.3745628804559	0.0
20	0.0	0.0	0.0	28.97293096749126	0.0
21	0.0	0.0	0.0	29.694340111384534	0.0
22	0.0	0.0	0.0	30.415749255277813	0.0
23	0.0	0.0	0.0	31.08405646936925	0.0
24	0.0	0.0	0.0	31.754954021499806	0.0
25	0.0	0.0	0.0	32.23287138971636	0.0
26	0.0	0.0	0.0	32.70431291283513	0.0
27	0.0	0.0	0.0	33.16021240771921	0.0
28	0.0	0.0	0.0	33.675689677502916	0.0
29	0.0	0.0	0.0	34.13417951042611	0.0
30	0.0	0.0	0.0	34.640590597072915	0.0
31	0.0	0.0	0.0	35.14441134568061	0.0
32	0.0	0.0	0.0	35.617148037818936	0.0
33	0.0	0.0	0.0	36.09377023701593	0.0
34	0.0	0.0	0.0	36.548374562880454	0.0
35	0.0	0.0	0.0	36.98484652247118	0.0
36	0.0	0.0	0.0	37.46017355264862	0.0
37	0.0	0.0	0.0	37.903121357337135	0.0
38	0.0	0.0	0.0	38.36679186633856	0.0
39	0.0	0.0	0.0	38.81362517808574	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACT	40	6.673872E-9	45.0	3
CGTGGGG	40	6.673872E-9	45.0	4
CAACGCA	35	1.1923476E-7	45.0	17
ACGCAAG	35	1.1923476E-7	45.0	19
TTATGAC	35	1.1923476E-7	45.0	27
GGGTAAT	20	6.9924275E-4	45.0	8
AATGGGC	20	6.9924275E-4	45.0	4
ACTTATA	40	6.673872E-9	45.0	7
TGAAACC	50	2.1827873E-11	45.0	4
GACCCGC	25	3.856979E-5	45.0	31
ACGGGAC	25	3.856979E-5	45.0	5
AAACGGC	80	0.0	45.0	6
TCGTGGG	20	6.9924275E-4	45.0	3
TGACCCG	25	3.856979E-5	45.0	30
CTTATGA	35	1.1923476E-7	45.0	26
AACGCAA	35	1.1923476E-7	45.0	18
ACTAATC	20	6.9924275E-4	45.0	10
GACGGGA	20	6.9924275E-4	45.0	4
AGGGGTT	25	3.856979E-5	45.0	6
TGTAGGG	35	1.1923476E-7	45.0	3
>>END_MODULE