##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936343.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125979
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.105850975162525	33.0	31.0	34.0	30.0	34.0
2	32.232411751164875	33.0	31.0	34.0	30.0	34.0
3	32.08226767953389	33.0	31.0	34.0	30.0	34.0
4	35.82225609030076	37.0	35.0	37.0	35.0	37.0
5	35.91062796180316	37.0	35.0	37.0	35.0	37.0
6	35.88352026925122	37.0	35.0	37.0	35.0	37.0
7	27.553901840782988	37.0	26.0	37.0	0.0	37.0
8	31.529262813643545	37.0	28.0	37.0	17.0	37.0
9	36.146492669413156	39.0	32.0	39.0	32.0	39.0
10	36.88176600862048	38.0	37.0	39.0	34.0	39.0
11	37.17052842140357	39.0	37.0	39.0	34.0	39.0
12	37.15328745267068	39.0	37.0	39.0	34.0	39.0
13	37.08669698918074	39.0	37.0	39.0	33.0	39.0
14	38.155684677604995	40.0	38.0	41.0	33.0	41.0
15	38.25451067241366	40.0	38.0	41.0	33.0	41.0
16	38.26561569785441	40.0	37.0	41.0	33.0	41.0
17	38.1797124917645	40.0	37.0	41.0	33.0	41.0
18	38.081481834273966	40.0	37.0	41.0	33.0	41.0
19	37.96451789583978	40.0	37.0	41.0	33.0	41.0
20	38.01539145413125	40.0	37.0	41.0	33.0	41.0
21	37.97428142785702	40.0	36.0	41.0	33.0	41.0
22	38.018272886750964	40.0	36.0	41.0	34.0	41.0
23	37.993086149278845	40.0	36.0	41.0	34.0	41.0
24	37.93506060533898	40.0	36.0	41.0	34.0	41.0
25	37.80895228569841	40.0	36.0	41.0	33.0	41.0
26	37.692242357853296	40.0	36.0	41.0	33.0	41.0
27	37.59374181411187	39.0	36.0	41.0	33.0	41.0
28	37.53725620936823	39.0	36.0	41.0	33.0	41.0
29	37.470800689003724	39.0	36.0	41.0	33.0	41.0
30	37.32788798133022	39.0	35.0	41.0	33.0	41.0
31	37.06281205597758	39.0	35.0	41.0	31.0	41.0
32	36.94995991395391	39.0	35.0	41.0	31.0	41.0
33	36.86211193929147	39.0	35.0	41.0	31.0	41.0
34	36.487898776780256	39.0	35.0	41.0	30.0	41.0
35	36.45737781693774	39.0	35.0	41.0	30.0	41.0
36	36.149969439351004	39.0	35.0	41.0	29.0	41.0
37	36.076989021979855	39.0	35.0	41.0	29.0	41.0
38	35.907619523888904	39.0	35.0	40.0	28.0	41.0
39	35.63716968701133	39.0	35.0	40.0	26.0	41.0
40	35.40135260638678	38.0	34.0	40.0	25.0	41.0
41	35.150866414243644	38.0	34.0	40.0	24.0	41.0
42	35.20312115511315	38.0	34.0	40.0	25.0	41.0
43	35.26532993594171	38.0	34.0	40.0	25.0	41.0
44	35.079354495590536	38.0	34.0	40.0	24.0	41.0
45	34.9603346589511	38.0	34.0	40.0	24.0	41.0
46	34.82082728073726	38.0	34.0	40.0	24.0	41.0
47	34.576627850673525	38.0	33.0	40.0	23.0	41.0
48	34.47538875526874	37.0	33.0	40.0	23.0	41.0
49	34.33690535724208	37.0	33.0	40.0	23.0	41.0
50	34.124123861913496	37.0	33.0	40.0	22.0	41.0
51	31.874510831170273	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	13.0
10	11.0
11	9.0
12	5.0
13	6.0
14	3.0
15	5.0
16	9.0
17	30.0
18	40.0
19	85.0
20	144.0
21	228.0
22	382.0
23	570.0
24	867.0
25	1243.0
26	1587.0
27	1793.0
28	1968.0
29	2221.0
30	2659.0
31	3252.0
32	4150.0
33	5782.0
34	8934.0
35	10809.0
36	12066.0
37	18718.0
38	23574.0
39	24810.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.418347502361506	20.375618158581986	23.481691393009946	12.724342946046562
2	32.510180268139926	26.6314226974337	24.27706205002421	16.58133498440216
3	31.64654426531406	26.01227188658427	26.41392613054557	15.9272577175561
4	27.872899451495886	24.90891339032696	28.429341398169537	18.788845760007618
5	26.240087633653225	28.29519205581883	25.54632121226554	19.91839909826241
6	25.09227728430929	34.82802689337112	25.721747275339542	14.357948546980051
7	60.66249136760889	26.129751784027494	8.918153025504251	4.289603822859365
8	79.57834242214973	6.513784043372309	9.414267457274624	4.493606077203344
9	73.72895482580431	7.026567920050167	11.13836433056303	8.106112923582502
10	42.75077592297129	24.32468903547417	18.476889005310408	14.447646036244135
11	31.94897562292128	25.131966438850924	24.99226061486438	17.926797323363417
12	28.316624199271306	22.04097508314878	30.160582319275438	19.48181839830448
13	24.46360107636987	23.322140991752594	32.73164575048222	19.48261218139531
14	20.451821335301915	26.79573579723605	30.982941601378005	21.769501266084028
15	18.94125211344748	25.16054263012089	36.52196000920788	19.376245247223743
16	23.809523809523807	25.021630589225186	29.984362473110597	21.184483128140403
17	23.914303177513712	24.24054802784591	28.81511998031418	23.030028814326197
18	24.32627660165583	24.650933885806364	30.528897673421763	20.493891839116042
19	24.21038427039427	26.738583414696098	27.880043499313377	21.17098881559625
20	24.196096174759283	27.42203065590297	29.201692345549656	19.180180823788092
21	24.895419077782805	26.781447701601063	29.970868160566443	18.352265060049692
22	23.607109121361496	23.21339270830853	30.187570944363745	22.991927225966233
23	21.819509600806484	26.068630486033385	30.79402122575985	21.317838687400283
24	23.41660117956167	24.30960715674835	29.499361004611877	22.774430659078103
25	22.326736995848513	27.89115646258503	28.075314139658197	21.706792401908253
26	21.920320053342223	26.078155883123376	28.20708213273641	23.79444193079799
27	22.384683161479295	26.74175854705943	29.34616086808119	21.527397423380087
28	19.650894196651823	25.64871923098294	30.64082108922916	24.059565483136076
29	22.960175902332928	25.434397796458143	29.361242746807008	22.244183554401925
30	24.108780034767697	24.960509291231077	28.629374737059347	22.30133593694187
31	25.824145294056944	25.264528215019965	27.679216377332732	21.23211011359036
32	27.275180784098936	25.528064201176388	27.023551544305	20.173203470419672
33	26.43615205708888	24.6517276688972	26.444089887997208	22.468030386016714
34	23.48883544082744	24.404067344557426	31.28934187443939	20.817755340175744
35	23.586470760999852	25.471705601727272	27.88401241476754	23.05781122250534
36	26.160709324569968	25.893998206050217	27.60380698370363	20.341485485676184
37	24.299287976567523	26.48616039181133	27.710173917875203	21.504377713745942
38	26.246437898379888	26.57030139943959	25.94400654077267	21.239254161407853
39	24.9732098206844	25.09624619976345	27.91814508767334	22.012398891878803
40	26.856857095230158	23.19831082958271	29.309646845902886	20.635185229284243
41	21.63535192373332	26.275807872740696	28.300748537454655	23.788091666071328
42	22.710928011811493	24.711261400709642	30.084379142555505	22.49343144492336
43	23.27530778939347	25.56140309099136	28.060232260932377	23.1030568586828
44	23.39199390374586	24.626326609990553	28.11976599274482	23.86191349351876
45	22.91731161542797	23.91112804515038	27.692710689876883	25.478849649544767
46	24.26753665293422	25.35184435501155	27.84114812786258	22.539470864191653
47	21.60121925082752	24.783495661975408	31.587010533501612	22.028274553695457
48	22.38944586002429	25.830495558783607	29.034997896474806	22.745060684717295
49	23.420570095015837	23.39199390374586	30.624945427412502	22.562490573825798
50	21.63297057446082	25.22960175902333	29.24376284936378	23.893664817152064
51	22.326736995848513	24.023051460957777	27.607775899157794	26.042435644035912
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	66.0
1	168.0
2	270.0
3	667.5
4	1065.0
5	725.5
6	386.0
7	339.5
8	293.0
9	313.0
10	333.0
11	330.5
12	328.0
13	337.5
14	347.0
15	328.0
16	309.0
17	322.0
18	335.0
19	356.5
20	378.0
21	421.0
22	464.0
23	543.5
24	623.0
25	769.5
26	1049.5
27	1183.0
28	1346.0
29	1509.0
30	1882.5
31	2256.0
32	2730.0
33	3204.0
34	3584.5
35	3965.0
36	4105.5
37	4246.0
38	4583.0
39	4920.0
40	5295.5
41	5671.0
42	6083.5
43	6496.0
44	6803.0
45	7110.0
46	7604.0
47	8098.0
48	9839.0
49	11580.0
50	11106.0
51	10632.0
52	10268.5
53	9905.0
54	9013.5
55	8122.0
56	7628.0
57	7134.0
58	6611.0
59	6088.0
60	5532.0
61	4976.0
62	4549.5
63	4123.0
64	3541.5
65	2960.0
66	2488.5
67	2017.0
68	1727.5
69	1438.0
70	1181.0
71	924.0
72	733.0
73	542.0
74	446.0
75	283.5
76	217.0
77	156.0
78	95.0
79	64.5
80	34.0
81	30.0
82	26.0
83	25.5
84	25.0
85	16.0
86	7.0
87	5.5
88	4.0
89	4.0
90	4.0
91	3.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	125979.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.947380118908704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.83339825187355	36.08776065852245
2	10.711559135596653	10.914517498948237
3	5.7242572020628515	8.749077227156906
4	3.6614056681675833	7.461561053826431
5	2.6034931368119283	6.632057723906365
6	2.0207843198354705	6.177220012859285
7	1.4037985136251032	5.006389953881203
8	0.970662013305704	3.956214924709674
9	0.663727155165698	3.043364370252185
>10	1.3710795693563715	8.724469951341096
>50	0.01869653958213234	0.7318680097476563
>100	0.01246435972142156	1.1295533382547887
>500	0.004674134895533085	1.385945276593718
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	661	0.524690623040348	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGC	575	0.45642527722874443	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	510	0.4048293763246255	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	426	0.33815159669468725	No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	195	0.1547877027123568	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	186	0.14764365489486345	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTT	132	0.10477936798990307	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12859286071488105	0.0
2	0.0	0.0	0.0	0.6874161566610308	0.0
3	0.0	0.0	0.0	0.9112629882758237	0.0
4	0.0	0.0	0.0	1.257352415878837	0.0
5	0.0	0.0	0.0	2.4623151477627223	0.0
6	0.0	0.0	0.0	2.90445232935648	0.0
7	0.0	0.0	0.0	3.381515966946872	0.0
8	0.0	0.0	0.0	4.0617880757904095	0.0
9	0.0	0.0	0.0	4.332468109764326	0.0
10	0.0	0.0	0.0	5.766834154898833	0.0
11	0.0	0.0	0.0	6.717786297716286	0.0
12	0.0	0.0	0.0	8.168028004667445	0.0
13	0.0	0.0	0.0	8.517292564633788	0.0
14	0.0	0.0	0.0	8.725263734431929	0.0
15	0.0	0.0	0.0	9.030076441311648	0.0
16	0.0	0.0	0.0	9.488883067812889	0.0
17	0.0	0.0	0.0	9.973884536311607	0.0
18	0.0	0.0	0.0	10.509688122623611	0.0
19	0.0	0.0	0.0	10.856571333317458	0.0
20	0.0	0.0	0.0	11.169321871105502	0.0
21	0.0	0.0	0.0	11.567800982703467	0.0
22	0.0	0.0	0.0	11.99326871938974	0.0
23	0.0	0.0	0.0	12.377459735352717	0.0
24	0.0	0.0	0.0	12.718786464410735	0.0
25	0.0	0.0	0.0	13.028361869835448	0.0
26	0.0	0.0	0.0	13.299041903809366	0.0
27	0.0	0.0	0.0	13.641956199049048	0.0
28	0.0	0.0	0.0	13.92374919629462	0.0
29	0.0	0.0	0.0	14.242056215718494	0.0
30	0.0	0.0	0.0	14.668317735495599	0.0
31	0.0	0.0	0.0	14.97074909310282	0.0
32	0.0	0.0	0.0	15.316838520705831	0.0
33	0.0	0.0	0.0	15.610538264313893	0.0
34	0.0	0.0	0.0	15.922495019011105	0.0
35	0.0	0.0	0.0	16.308273601155747	0.0
36	0.0	0.0	0.0	16.66547599203042	0.0
37	0.0	0.0	0.0	17.02029703363259	0.0
38	0.0	0.0	0.0	17.421157494503053	0.0
39	0.0	0.0	0.0	18.00935076481001	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGATG	30	2.1500855E-6	45.000004	6
CTCTAAC	30	2.1500855E-6	45.000004	23
CCAGCGG	20	7.0093625E-4	45.0	2
TACGGTT	40	6.73208E-9	45.0	33
ACGGGCT	20	7.0093625E-4	45.0	5
AATGCGG	20	7.0093625E-4	45.0	2
CGGGCAA	20	7.0093625E-4	45.0	6
CGTACGG	40	6.73208E-9	45.0	31
GATTGTC	40	6.73208E-9	45.0	23
TTACGGG	25	3.8709888E-5	45.0	3
GCAATAG	20	7.0093625E-4	45.0	1
CGTTTTA	25	3.8709888E-5	45.0	1
GTACGGT	40	6.73208E-9	45.0	32
TAGTAGG	25	3.8709888E-5	45.0	2
ACGGTTA	40	6.73208E-9	45.0	34
TACGGCT	115	0.0	44.999996	7
ACATACG	50	1.0659278E-9	40.5	17
CGGCTGT	145	0.0	40.34483	9
CTATGGG	45	1.9050276E-8	40.0	3
CTCGTAC	45	1.9050276E-8	40.0	29
>>END_MODULE