##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936341.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	181127
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86519955611256	31.0	31.0	34.0	30.0	34.0
2	31.9691597608308	33.0	31.0	34.0	30.0	34.0
3	31.696505766672004	33.0	31.0	34.0	30.0	34.0
4	35.5641014315922	37.0	35.0	37.0	33.0	37.0
5	35.71835231633053	37.0	35.0	37.0	35.0	37.0
6	35.69198959845854	37.0	35.0	37.0	35.0	37.0
7	27.013211724370194	35.0	18.0	37.0	0.0	37.0
8	31.222286020306193	35.0	23.0	37.0	17.0	37.0
9	35.93506213872035	39.0	32.0	39.0	32.0	39.0
10	36.61716916859441	38.0	35.0	39.0	32.0	39.0
11	36.9771155045907	39.0	37.0	39.0	33.0	39.0
12	37.03264560225698	39.0	37.0	39.0	33.0	39.0
13	36.97606099587582	39.0	37.0	39.0	33.0	39.0
14	38.07311996554903	40.0	37.0	41.0	33.0	41.0
15	38.157419931871004	40.0	37.0	41.0	33.0	41.0
16	38.14026070105506	40.0	37.0	41.0	33.0	41.0
17	38.04896012190341	40.0	37.0	41.0	33.0	41.0
18	37.91372904094917	40.0	37.0	41.0	33.0	41.0
19	37.7520689902665	40.0	37.0	41.0	33.0	41.0
20	37.7788623452053	40.0	36.0	41.0	33.0	41.0
21	37.741507340153596	40.0	36.0	41.0	33.0	41.0
22	37.78287058251945	39.0	36.0	41.0	33.0	41.0
23	37.73206092962396	39.0	36.0	41.0	33.0	41.0
24	37.64903079055028	39.0	36.0	41.0	33.0	41.0
25	37.55064678374842	39.0	35.0	41.0	33.0	41.0
26	37.430002153185335	39.0	35.0	41.0	33.0	41.0
27	37.33193284270153	39.0	35.0	41.0	33.0	41.0
28	37.32462305454184	39.0	35.0	41.0	33.0	41.0
29	37.25515798307265	39.0	35.0	41.0	32.0	41.0
30	37.13669966377183	39.0	35.0	41.0	32.0	41.0
31	36.98976408818122	39.0	35.0	41.0	31.0	41.0
32	36.83292938104203	39.0	35.0	41.0	31.0	41.0
33	36.65008529926516	39.0	35.0	41.0	30.0	41.0
34	36.39391697538191	39.0	35.0	41.0	30.0	41.0
35	36.249846792582	39.0	35.0	40.0	30.0	41.0
36	36.03754823963296	39.0	35.0	40.0	29.0	41.0
37	35.93881641058484	39.0	35.0	40.0	29.0	41.0
38	35.752748071794926	39.0	35.0	40.0	27.0	41.0
39	35.6412793233477	39.0	35.0	40.0	27.0	41.0
40	35.380633478167255	38.0	35.0	40.0	25.0	41.0
41	35.315309147725074	38.0	34.0	40.0	25.0	41.0
42	35.27104738664031	38.0	34.0	40.0	25.0	41.0
43	35.323656881635536	38.0	34.0	40.0	25.0	41.0
44	35.123366477664845	38.0	34.0	40.0	24.0	41.0
45	35.00272184710176	38.0	34.0	40.0	24.0	41.0
46	34.870085630524436	38.0	34.0	40.0	23.0	41.0
47	34.64658499285032	38.0	34.0	40.0	23.0	41.0
48	34.58402667741419	38.0	33.0	40.0	23.0	41.0
49	34.43496552142972	38.0	33.0	40.0	23.0	41.0
50	34.18895029454471	37.0	33.0	40.0	22.0	41.0
51	31.95039392249637	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	9.0
10	15.0
11	10.0
12	7.0
13	3.0
14	5.0
15	12.0
16	23.0
17	41.0
18	72.0
19	140.0
20	249.0
21	395.0
22	597.0
23	890.0
24	1363.0
25	1973.0
26	2499.0
27	2803.0
28	3072.0
29	3479.0
30	4156.0
31	4976.0
32	6511.0
33	8403.0
34	12546.0
35	15262.0
36	17414.0
37	26440.0
38	32996.0
39	34753.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.870472099686964	19.76403297134055	23.220723580692002	14.144771348280488
2	32.65112324501593	25.01449259359455	24.97971036896763	17.35467379242189
3	29.94749540377746	25.791847709066012	28.327637514009506	15.933019373147017
4	26.88169074737615	24.359151313719103	30.85404163929177	17.905116299612978
5	25.14257951603019	28.60810370623927	27.203564349876054	19.045752427854488
6	23.49842927890375	34.98098019621592	27.571814251878518	13.948776273001817
7	59.096103838742984	27.02799693033065	9.760554748877858	4.115344482048507
8	79.06220497220183	5.783787066533427	10.72893605039558	4.4250719108691685
9	73.76260855642725	6.887984673737211	12.120225035472348	7.2291817343631815
10	38.28253104175523	29.792907738768932	18.893373158060367	13.031188061415472
11	27.89037526155681	25.68584473877445	28.56448790075472	17.859292098914022
12	26.472033435103548	22.04751362303798	32.31047828319356	19.169974658664916
13	23.449292485383182	23.666267315198727	34.3344724971981	18.549967702219988
14	20.11130311880614	26.67244530081103	32.32648914849802	20.889762431884808
15	18.192759776289567	26.344498611471508	36.48820993004908	18.974531682189845
16	22.756960585666413	25.958581547753784	31.00145201985347	20.28300584672633
17	22.85854676552916	24.63299232030564	30.59731569561689	21.911145218548313
18	22.847504789457123	25.523527690515497	31.575634775599443	20.053332744427944
19	22.760825277291623	27.20301225107245	29.22148547704097	20.81467699459495
20	22.696229717270203	27.749590066638323	30.25391023977651	19.30026997631496
21	23.337216428252	26.548775168804212	31.63967823681726	18.474330166126528
22	21.928812380263572	23.59615076714129	31.297929077387685	23.17710777520745
23	20.82295847664898	26.167274895515302	31.63912613801366	21.37064048982206
24	21.734473601395706	25.41642052261673	30.789446079270345	22.05965979671722
25	20.98582762371154	28.171393552590175	29.333561534172155	21.509217289526134
26	20.29515202040557	27.335515963936906	29.08677336896211	23.28255864669541
27	20.45802116746813	27.5955545004334	29.58642278622182	22.36000154587665
28	18.975083780993447	25.67811535552402	31.264251050367974	24.082549813114554
29	21.138758992309263	26.803292717264682	29.05309534194239	23.004852948483663
30	21.640616804783384	26.09494995224345	30.031414421924946	22.233018821048216
31	22.15517288974035	25.822765242067717	28.476152092178413	23.545909776013517
32	23.245015928050485	27.15718805037349	26.854637905999656	22.743158115576364
33	22.78014873541769	25.358450148238525	27.67560882695567	24.185792289388107
34	20.45802116746813	26.479210719550373	30.363225802889687	22.699542310091815
35	20.25429670893903	27.84786365367946	28.08581823803188	23.81202139934963
36	21.717358538484046	28.922247925488747	27.285827071612733	22.07456646441447
37	19.7491263036433	27.58175203034335	29.044261761084766	23.624859904928584
38	21.49320642422168	27.30239003572079	27.50059350621387	23.703810033843656
39	21.930468676674376	26.260027494520422	28.66110519138505	23.148398637420154
40	22.94080948726584	26.205369712963833	29.76364650217803	21.0901742975923
41	19.794950504342257	27.49672881458866	28.88856989846903	23.819750782600053
42	19.788877417502636	27.318400901025246	30.169991221629022	22.722730459843092
43	20.649047353514387	26.456022569799092	28.913414344631118	23.98151573205541
44	21.209979737973907	25.611863499091797	28.341439984099555	24.83671677883474
45	20.290183131173155	25.40537854654469	28.329293810420314	25.975144511861842
46	22.446128959238546	26.67244530081103	27.5972107968442	23.284214943106218
47	18.94416624799174	26.16451440149729	31.313939942692144	23.577379407818825
48	20.55132586527685	26.474793929121553	29.138670656500686	23.83520954910091
49	20.49335549089865	25.00841950675493	30.53658482722068	23.96164017512574
50	20.22227497833012	25.198341495193983	30.165022332396607	24.41436119407929
51	19.656925803441784	25.716762271776155	28.303345166651024	26.322966758131034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	60.0
1	194.5
2	329.0
3	1462.0
4	2595.0
5	1792.0
6	989.0
7	851.0
8	713.0
9	703.0
10	693.0
11	708.5
12	724.0
13	704.0
14	684.0
15	663.0
16	642.0
17	604.0
18	566.0
19	577.5
20	589.0
21	693.5
22	798.0
23	853.5
24	909.0
25	1089.0
26	1617.5
27	1966.0
28	2344.5
29	2723.0
30	3121.5
31	3520.0
32	3994.5
33	4469.0
34	5063.5
35	5658.0
36	6090.0
37	6522.0
38	7156.5
39	7791.0
40	8434.0
41	9077.0
42	9789.5
43	10502.0
44	11497.5
45	12493.0
46	15359.5
47	18226.0
48	17254.0
49	16282.0
50	15395.5
51	14509.0
52	12963.5
53	11418.0
54	10206.0
55	8994.0
56	8431.0
57	7868.0
58	7381.5
59	6895.0
60	6197.5
61	5500.0
62	4918.0
63	4336.0
64	4001.0
65	3666.0
66	3107.0
67	2548.0
68	2138.0
69	1728.0
70	1382.0
71	1036.0
72	887.5
73	739.0
74	604.0
75	377.5
76	286.0
77	227.0
78	168.0
79	127.0
80	86.0
81	68.0
82	50.0
83	36.5
84	23.0
85	17.0
86	11.0
87	8.0
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	181127.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.766291055447276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8558603804382	38.970998249846794
2	10.17222257099211	10.735009137235199
3	5.0683240211773075	8.023099813942704
4	3.118002804109904	6.58101773893456
5	2.165861008223994	5.714222617279589
6	1.467972461129596	4.647567728720732
7	1.1090882457572144	4.096573122726043
8	0.8464645196392324	3.573183456911449
9	0.6079059158348504	2.8869246440342966
>10	1.549584615062674	10.504783936133212
>50	0.020926193316173857	0.709446962628432
>100	0.013602025655513005	1.2968800896608457
>500	0.0031389289974260784	1.4658223235630248
>1k	0.0010463096658086927	0.7944701783831234
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1439	0.7944701783831234	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC	968	0.5344316418866321	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCG	883	0.48750324358047115	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGC	804	0.4438874380959216	No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	318	0.17556741954540184	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	229	0.1264306260248334	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCG	228	0.1258785272212315	No Hit
GAATCTATCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC	208	0.11483655114919367	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCT	203	0.1120760571311842	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14023309611488072	0.0
2	0.0	0.0	0.0	0.6349136241421764	0.0
3	0.0	0.0	0.0	0.8336691934388578	0.0
4	0.0	0.0	0.0	1.1654805744035952	0.0
5	0.0	0.0	0.0	2.450766589188801	0.0
6	0.0	0.0	0.0	3.0304703329707885	0.0
7	0.0	0.0	0.0	3.6162471635923965	0.0
8	0.0	0.0	0.0	4.657505507185566	0.0
9	0.0	0.0	0.0	5.093663562031061	0.0
10	0.0	0.0	0.0	6.585434529363375	0.0
11	0.0	0.0	0.0	7.551607435666687	0.0
12	0.0	0.0	0.0	8.936823333903835	0.0
13	0.0	0.0	0.0	9.357522622248478	0.0
14	0.0	0.0	0.0	9.551309302312742	0.0
15	0.0	0.0	0.0	9.853307347882977	0.0
16	0.0	0.0	0.0	10.362342444803922	0.0
17	0.0	0.0	0.0	10.926035323281454	0.0
18	0.0	0.0	0.0	11.605668950515383	0.0
19	0.0	0.0	0.0	12.011461571162775	0.0
20	0.0	0.0	0.0	12.469151479348744	0.0
21	0.0	0.0	0.0	12.94671694446438	0.0
22	0.0	0.0	0.0	13.512066119352719	0.0
23	0.0	0.0	0.0	14.043737267221342	0.0
24	0.0	0.0	0.0	14.482655816084847	0.0
25	0.0	0.0	0.0	14.884031646303422	0.0
26	0.0	0.0	0.0	15.271605006431951	0.0
27	0.0	0.0	0.0	15.71162775290266	0.0
28	0.0	0.0	0.0	16.104722101067207	0.0
29	0.0	0.0	0.0	16.53149447625147	0.0
30	0.0	0.0	0.0	17.059300932494878	0.0
31	5.520988036018926E-4	0.0	0.0	17.49656318494758	0.0
32	5.520988036018926E-4	0.0	0.0	17.942659018257906	0.0
33	5.520988036018926E-4	0.0	0.0	18.367775097031366	0.0
34	5.520988036018926E-4	0.0	0.0	18.798964262644443	0.0
35	5.520988036018926E-4	0.0	0.0	19.254445775616006	0.0
36	5.520988036018926E-4	0.0	0.0	19.649748518994958	0.0
37	5.520988036018926E-4	0.0	0.0	20.105782130770123	0.0
38	5.520988036018926E-4	0.0	0.0	20.58886858392178	0.0
39	5.520988036018926E-4	0.0	0.0	21.22157381284955	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAG	20	7.0175383E-4	45.0	6
CTCACGA	20	7.0175383E-4	45.0	24
TTGGCAA	20	7.0175383E-4	45.0	1
TAGACGG	40	6.7611836E-9	45.0	2
TACGGTT	20	7.0175383E-4	45.0	33
ATATGAG	20	7.0175383E-4	45.0	1
ATCAACC	20	7.0175383E-4	45.0	10
CGTTAAG	20	7.0175383E-4	45.0	2
AATGCGG	20	7.0175383E-4	45.0	2
TCTCGTG	20	7.0175383E-4	45.0	43
TCGTACG	20	7.0175383E-4	45.0	30
CGTCACG	20	7.0175383E-4	45.0	36
GTACGGT	20	7.0175383E-4	45.0	32
ACGGTTA	20	7.0175383E-4	45.0	34
CGTTTTA	90	0.0	42.5	1
CGTTTTT	545	0.0	42.11009	1
GACGGGA	65	0.0	41.538464	4
CTCGTCC	60	3.6379788E-12	41.249996	37
AAGGGCG	45	1.9128493E-8	40.0	5
AGGCGAT	40	3.4367622E-7	39.375	7
>>END_MODULE