##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936340.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86388
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.973086539797194	33.0	31.0	34.0	30.0	34.0
2	32.07227855720702	33.0	31.0	34.0	30.0	34.0
3	31.96524980321341	33.0	31.0	34.0	30.0	34.0
4	35.73107376024448	37.0	35.0	37.0	35.0	37.0
5	35.81440709357781	37.0	35.0	37.0	35.0	37.0
6	35.780177802472565	37.0	35.0	37.0	35.0	37.0
7	26.59309163309719	35.0	10.0	37.0	0.0	37.0
8	30.98393295365097	35.0	19.0	37.0	17.0	37.0
9	35.80989257767283	38.0	32.0	39.0	32.0	39.0
10	36.69792100754734	38.0	35.0	39.0	33.0	39.0
11	37.05707968699356	39.0	37.0	39.0	34.0	39.0
12	37.06005463721813	39.0	37.0	39.0	34.0	39.0
13	36.93500254665	39.0	37.0	39.0	33.0	39.0
14	38.137646432374865	40.0	37.0	41.0	33.0	41.0
15	38.201879890725564	40.0	37.0	41.0	33.0	41.0
16	38.16521970644071	40.0	37.0	41.0	33.0	41.0
17	38.05571375654026	40.0	37.0	41.0	33.0	41.0
18	37.953002731860906	40.0	37.0	41.0	33.0	41.0
19	37.83671343242117	40.0	36.0	41.0	33.0	41.0
20	37.921817845071075	40.0	36.0	41.0	33.0	41.0
21	37.89652498032134	40.0	36.0	41.0	33.0	41.0
22	37.93716719914803	40.0	36.0	41.0	33.0	41.0
23	37.91952586007316	40.0	36.0	41.0	33.0	41.0
24	37.86246932444321	40.0	36.0	41.0	33.0	41.0
25	37.76317312589712	40.0	36.0	41.0	33.0	41.0
26	37.55174329768023	39.0	36.0	41.0	33.0	41.0
27	37.46612955503079	39.0	36.0	41.0	33.0	41.0
28	37.42882113256471	39.0	36.0	41.0	33.0	41.0
29	37.379902301245544	39.0	36.0	41.0	33.0	41.0
30	37.373142103069874	39.0	36.0	41.0	33.0	41.0
31	37.19530490345881	39.0	35.0	41.0	32.0	41.0
32	37.071711348798445	39.0	35.0	41.0	32.0	41.0
33	36.99143399546233	39.0	35.0	41.0	31.0	41.0
34	36.76922720748252	39.0	35.0	41.0	31.0	41.0
35	36.708281242765196	39.0	35.0	41.0	31.0	41.0
36	36.47740426911145	39.0	35.0	41.0	30.0	41.0
37	36.318296522665186	39.0	35.0	41.0	30.0	41.0
38	36.22099134139001	39.0	35.0	40.0	30.0	41.0
39	36.21235588276149	39.0	35.0	40.0	30.0	41.0
40	35.89618928554892	38.0	35.0	40.0	29.0	41.0
41	35.834502477195905	38.0	35.0	40.0	28.0	41.0
42	35.70965874890031	38.0	35.0	40.0	28.0	41.0
43	35.666666666666664	38.0	35.0	40.0	28.0	41.0
44	35.40534564985877	38.0	34.0	40.0	26.0	41.0
45	35.30587581608557	38.0	34.0	40.0	26.0	41.0
46	35.15561189054035	38.0	34.0	40.0	26.0	41.0
47	34.94147335278048	38.0	34.0	40.0	24.0	41.0
48	34.816409686530534	38.0	34.0	40.0	24.0	41.0
49	34.656341158494236	38.0	34.0	40.0	24.0	41.0
50	34.43741028846599	37.0	33.0	40.0	23.0	41.0
51	32.038303931101545	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	4.0
11	2.0
12	0.0
13	9.0
14	7.0
15	5.0
16	4.0
17	17.0
18	36.0
19	55.0
20	106.0
21	142.0
22	249.0
23	325.0
24	520.0
25	715.0
26	855.0
27	1100.0
28	1378.0
29	1558.0
30	1861.0
31	2326.0
32	2957.0
33	4050.0
34	6298.0
35	7719.0
36	8282.0
37	12816.0
38	16118.0
39	16867.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.582719822197525	19.79673102745752	22.094503866277723	12.52604528406723
2	30.896652312821228	27.822151224707138	24.307774227902023	16.973422234569615
3	32.11209890262536	27.297772838820205	25.090290318099733	15.499837940454691
4	28.51784970134741	25.068296522665186	27.721442792980504	18.692410983006898
5	27.00953836180951	29.182293837107004	23.630596842154002	20.17757095892948
6	24.938648886419408	36.39047089873594	24.599481409455016	14.071398805389638
7	57.20354678890587	28.903319905542435	9.169097559846275	4.724035745705422
8	75.88669722646664	8.745427605685975	9.940037968236329	5.427837199611057
9	68.86604621012178	9.92267444552484	11.660184284854378	9.551095059499005
10	41.06125850812613	26.63101356669908	18.796592119275825	13.511135805898967
11	33.65397971940548	24.83562531833125	23.791498819280456	17.71889614298282
12	33.05204426540723	21.203176367088023	27.62536463397694	18.119414733527805
13	24.35870722785572	26.293003657915452	29.344353382414223	20.003935731814604
14	18.65305366486086	30.815622540167613	26.546511089503174	23.984812705468354
15	18.406491642357732	26.8335879983331	36.12191508079826	18.638005278510906
16	21.013335185442422	25.701486317544102	31.67222299393434	21.61295550307913
17	20.982080844561747	24.689771727554753	29.073482428115017	25.25466499976849
18	21.081631708107608	27.881187201926195	28.86627772375793	22.170903366208268
19	24.667777932120202	28.25855442885586	25.59846274945594	21.475204889567994
20	26.737509839329537	26.2744825670232	28.7655692920313	18.222438301615966
21	24.394591841459462	28.669491133027737	26.650692225772097	20.285224799740707
22	21.98916516182803	26.260591748854008	26.717831180256518	25.03241190906144
23	23.029818956336527	28.418298837801547	27.406584247812194	21.14529795804973
24	24.61800250034727	24.13645413714868	28.376626383293974	22.868916979210073
25	21.37102375329907	29.226281427976108	26.275640135203965	23.12705468352086
26	20.87442700375052	29.726350882066953	26.75255822567949	22.64666388850303
27	23.53104597860814	29.464740473213872	26.046441635412325	20.95777191276566
28	19.59184145946196	28.845441496504144	30.316710654257534	21.24600638977636
29	22.37926563874612	29.314256609714313	27.479511043200443	20.82696670833912
30	24.056581932675837	27.403111543269898	27.801314997453353	20.738991526600916
31	23.644487660323193	27.193591702551277	27.8198360883456	21.342084548779923
32	26.411075612353567	29.47863129138306	26.42149372598046	17.688799370282908
33	26.090429226281426	28.010834838171967	24.962957818215493	20.93577811733111
34	23.00666759272121	27.93559290642219	29.432328564152428	19.62541093670417
35	26.123998703523636	24.65157197758948	26.055702180858454	23.16872713802843
36	25.494281613187013	28.222669815252114	26.8301152937908	19.45293327777006
37	24.40964022780942	27.066259202667037	30.000694540908455	18.523406028615085
38	24.21401120525999	26.657637634856695	27.162337361670602	21.966013798212714
39	25.056720840857526	24.587905727647357	30.38616474510349	19.969208686391628
40	27.12413761170533	23.867898319211	29.3385655415104	19.66939852757327
41	21.043431958142335	27.239894429781913	28.950780200953837	22.765893411121915
42	23.587766819465667	26.081168680835304	28.71232115571607	21.61874334398296
43	26.584710839468446	24.95022456822707	26.85558179376765	21.609482798536835
44	22.999722183636617	26.539565680418576	28.137009769875444	22.323702366069362
45	23.01592813816734	25.480390795017826	26.401815066907442	25.101865999907396
46	24.197805250729267	28.362735565124787	25.629717090336623	21.809742093809326
47	22.927952956429134	25.280131499745334	31.030930221790065	20.76098532203547
48	22.924480251886838	24.811316386535168	28.719266564800666	23.54493679677733
49	21.210121776172617	24.934018613696345	31.115432698985966	22.740426911145068
50	20.690373663008753	25.845024771959068	29.732138722970785	23.7324628420614
51	21.447423253229616	24.864564522850397	27.253785247951107	26.434226975968883
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	151.0
2	241.0
3	388.5
4	536.0
5	381.5
6	227.0
7	191.5
8	156.0
9	165.5
10	175.0
11	190.0
12	205.0
13	205.0
14	205.0
15	213.0
16	221.0
17	225.5
18	230.0
19	239.5
20	249.0
21	331.5
22	414.0
23	417.0
24	420.0
25	544.0
26	839.5
27	1011.0
28	1220.0
29	1429.0
30	1634.5
31	1840.0
32	2064.5
33	2289.0
34	2399.0
35	2509.0
36	2644.5
37	2780.0
38	3007.0
39	3234.0
40	3785.5
41	4337.0
42	4669.5
43	5002.0
44	5462.0
45	5922.0
46	7341.5
47	8761.0
48	7963.0
49	7165.0
50	6962.0
51	6759.0
52	5852.5
53	4946.0
54	4619.0
55	4292.0
56	4159.5
57	4027.0
58	4000.0
59	3973.0
60	3960.5
61	3948.0
62	3519.0
63	3090.0
64	2502.0
65	1914.0
66	1527.0
67	1140.0
68	993.5
69	847.0
70	636.0
71	425.0
72	365.5
73	306.0
74	237.0
75	138.0
76	108.0
77	95.5
78	83.0
79	60.5
80	38.0
81	26.0
82	14.0
83	13.0
84	12.0
85	9.0
86	6.0
87	3.5
88	1.0
89	0.5
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	86388.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.23253229615224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.55324351649385	34.735148400240774
2	10.789776868636993	10.624160763068945
3	5.645294020831864	8.337963606056396
4	3.719639792151607	7.325091447886281
5	2.649831887329242	6.522896698615549
6	1.9280054548447014	5.695235449367968
7	1.349603818391291	4.651108950317174
8	0.9969198937245775	3.9264712691577532
9	0.6818555876889798	3.0212529517988607
>10	1.6270485057957726	10.611427513080521
>50	0.03056594013778185	1.0927443626429596
>100	0.02351226164444758	2.0465805435940174
>500	0.0047024523288895165	1.4099180441728019
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	630	0.7292679538824837	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	588	0.6806500902903181	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	483	0.5591054313099041	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	224	0.2592952724915497	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	203	0.23498634069546698	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	142	0.1643746816687503	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	139	0.16090197712645274	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	129	0.1493262953187943	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	128	0.14816872713802842	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	109	0.12617493170347735	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	109	0.12617493170347735	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT	102	0.11807195443811641	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	92	0.10649627263045794	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15164143168032598	0.0
2	0.0	0.0	0.0	0.9630967263971848	0.0
3	0.0	0.0	0.0	1.2015557716349492	0.0
4	0.0	0.0	0.0	1.5928138167338057	0.0
5	0.0	0.0	0.0	3.165948974394592	0.0
6	0.0	0.0	0.0	3.596564337639487	0.0
7	0.0	0.0	0.0	4.226281427976108	0.0
8	0.0	0.0	0.0	5.101402972635088	0.0
9	0.0	0.0	0.0	5.43825531323795	0.0
10	0.0	0.0	0.0	7.154928925313701	0.0
11	0.0	0.0	0.0	8.123813492614715	0.0
12	0.0	0.0	0.0	9.905310922813353	0.0
13	0.0	0.0	0.0	10.279205445200722	0.0
14	0.0	0.0	0.0	10.478307172292448	0.0
15	0.0	0.0	0.0	10.863777376487475	0.0
16	0.0	0.0	0.0	11.48423392137797	0.0
17	0.0	0.0	0.0	12.096587489003102	0.0
18	0.0	0.0	0.0	12.70778348844747	0.0
19	0.0	0.0	0.0	13.11177478353475	0.0
20	0.0	0.0	0.0	13.50766310135667	0.0
21	0.0	0.0	0.0	13.963744964578414	0.0
22	0.0	0.0	0.0	14.454553873223134	0.0
23	0.0	0.0	0.0	14.932629531879428	0.0
24	0.0	0.0	0.0	15.29726350882067	0.0
25	0.0	0.0	0.0	15.648006667592721	0.0
26	0.0	0.0	0.0	16.0045376672686	0.0
27	0.0	0.0	0.0	16.302032689725426	0.0
28	0.0	0.0	0.0	16.58216418947076	0.0
29	0.0	0.0	0.0	16.89123489373524	0.0
30	0.0	0.0	0.0	17.29291105246099	0.0
31	0.0	0.0	0.0	17.676066120294486	0.0
32	0.0	0.0	0.0	18.060378756308747	0.0
33	0.0	0.0	0.0	18.412279483261564	0.0
34	0.0	0.0	0.0	18.72019261934528	0.0
35	0.0	0.0	0.0	19.074408482659628	0.0
36	0.0	0.0	0.0	19.473769505023846	0.0
37	0.0	0.0	0.0	19.88586377737649	0.0
38	0.0	0.0	0.0	20.2447099134139	0.0
39	0.0	0.0	0.0	20.590822799462888	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATTC	30	2.1421783E-6	45.000004	43
GAATCTA	25	3.8608086E-5	45.000004	1
TATGCTT	30	2.1421783E-6	45.000004	37
ACGGGAG	25	3.8608086E-5	45.000004	5
TGATTCA	30	2.1421783E-6	45.000004	44
TACGAAT	30	2.1421783E-6	45.000004	12
CGGGATA	25	3.8608086E-5	45.000004	6
CGAATAT	30	2.1421783E-6	45.000004	14
ATGAAAC	25	3.8608086E-5	45.000004	3
GCTACGA	25	3.8608086E-5	45.000004	10
AGCTACG	25	3.8608086E-5	45.000004	9
AGGGCGA	30	2.1421783E-6	45.000004	6
AAGGAAC	25	3.8608086E-5	45.000004	6
TGACTAT	30	2.1421783E-6	45.000004	23
ACACGGG	20	6.997062E-4	45.0	3
TCCGCCC	20	6.997062E-4	45.0	35
CACGGGA	20	6.997062E-4	45.0	4
GGCCGAT	20	6.997062E-4	45.0	8
TTTCCGC	20	6.997062E-4	45.0	33
TAACGGG	20	6.997062E-4	45.0	3
>>END_MODULE