##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936338.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136260
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.900733891090564	33.0	31.0	34.0	30.0	34.0
2	32.02984001174226	33.0	31.0	34.0	30.0	34.0
3	31.56443563775136	33.0	31.0	34.0	28.0	34.0
4	35.481704095112285	37.0	35.0	37.0	33.0	37.0
5	35.71467782181124	37.0	35.0	37.0	35.0	37.0
6	35.74524438573316	37.0	35.0	37.0	35.0	37.0
7	27.001218259210333	35.0	16.0	37.0	0.0	37.0
8	31.27172317628064	36.0	23.0	37.0	17.0	37.0
9	36.05581241743725	39.0	32.0	39.0	32.0	39.0
10	36.748745046235136	38.0	35.0	39.0	33.0	39.0
11	37.00743431674739	39.0	37.0	39.0	34.0	39.0
12	37.187098194627914	39.0	37.0	39.0	34.0	39.0
13	37.233083810362544	39.0	37.0	39.0	34.0	39.0
14	38.51282107735212	40.0	38.0	41.0	34.0	41.0
15	38.56196976368707	40.0	38.0	41.0	34.0	41.0
16	38.49178775869661	40.0	38.0	41.0	34.0	41.0
17	38.384683692939966	40.0	38.0	41.0	34.0	41.0
18	38.2228533685601	40.0	37.0	41.0	34.0	41.0
19	38.03986496403934	40.0	37.0	41.0	34.0	41.0
20	38.011478056656394	40.0	36.0	41.0	34.0	41.0
21	37.98483047115808	40.0	36.0	41.0	33.0	41.0
22	37.99671950682519	40.0	36.0	41.0	34.0	41.0
23	37.94147952443857	40.0	36.0	41.0	34.0	41.0
24	37.874445912226626	40.0	36.0	41.0	33.0	41.0
25	37.77140026420079	40.0	36.0	41.0	33.0	41.0
26	37.700051372376336	40.0	36.0	41.0	33.0	41.0
27	37.59431234404814	39.0	36.0	41.0	33.0	41.0
28	37.52197269925143	40.0	36.0	41.0	33.0	41.0
29	37.481256421547045	40.0	36.0	41.0	33.0	41.0
30	37.37261852341113	40.0	36.0	41.0	33.0	41.0
31	37.246183766329075	39.0	35.0	41.0	32.0	41.0
32	37.0618816967562	39.0	35.0	41.0	32.0	41.0
33	36.83628357551739	39.0	35.0	41.0	31.0	41.0
34	36.61086158814032	39.0	35.0	41.0	30.0	41.0
35	36.398899163364156	39.0	35.0	41.0	30.0	41.0
36	36.14351974167034	39.0	35.0	41.0	29.0	41.0
37	36.050983414061356	39.0	35.0	41.0	28.0	41.0
38	35.89140613532952	39.0	35.0	41.0	27.0	41.0
39	35.77768971084691	39.0	35.0	41.0	26.0	41.0
40	35.56978570380156	39.0	35.0	41.0	25.0	41.0
41	35.46339351240276	39.0	35.0	41.0	24.0	41.0
42	35.39929546455306	39.0	35.0	40.0	24.0	41.0
43	35.29014384265375	39.0	35.0	40.0	23.0	41.0
44	35.10851313665052	39.0	35.0	40.0	23.0	41.0
45	34.995435197416704	38.0	34.0	40.0	23.0	41.0
46	34.77796125055042	38.0	34.0	40.0	22.0	41.0
47	34.60144576544841	38.0	34.0	40.0	21.0	41.0
48	34.53103625421987	38.0	34.0	40.0	20.0	41.0
49	34.38088947600176	38.0	34.0	40.0	20.0	41.0
50	34.20934977249376	38.0	33.0	40.0	19.0	41.0
51	32.1471304858359	36.0	30.0	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	11.0
10	8.0
11	6.0
12	1.0
13	1.0
14	2.0
15	6.0
16	12.0
17	24.0
18	43.0
19	87.0
20	153.0
21	290.0
22	515.0
23	749.0
24	1120.0
25	1710.0
26	2147.0
27	2381.0
28	2440.0
29	2474.0
30	2781.0
31	3300.0
32	4133.0
33	5412.0
34	7957.0
35	10556.0
36	12065.0
37	19694.0
38	25678.0
39	30495.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.114633788345806	21.14853955672978	24.452517246440628	16.28430940848378
2	34.020989285190076	24.9398209305739	25.435931307793926	15.603258476442097
3	29.291795097607515	24.470864523704684	30.935711140466754	15.301629238221048
4	25.220167327168646	24.66020842506972	32.61191839131073	17.507705856450904
5	24.272713929252898	28.51460443270219	29.511228533685603	17.701453104359313
6	23.860267136356963	33.4155291354763	29.179509760751504	13.544693967415236
7	60.04036400998092	26.343020695728754	10.369881109643329	3.246734184646998
8	82.02333773667988	4.189050344928813	10.997357992073976	2.7902539263173343
9	76.35109349772495	6.729781300455012	12.104799647732277	4.814325554087773
10	35.366211654190515	35.89167767503302	18.300308234258036	10.44180243651842
11	21.532364597093792	26.14120064582416	35.33465433729634	16.991780419785705
12	22.818141787758698	22.82988404520769	36.26302656685748	18.088947600176134
13	20.78379568472039	25.266402465874066	36.525025686188165	17.424776163217377
14	18.07060032291208	28.51533832379275	33.18875678849258	20.22530456480258
15	16.539703507999413	28.601937472479083	36.834727726405404	18.0236312931161
16	20.46602084250697	28.769998532217816	33.18728900631147	17.576691618963746
17	21.02451196242478	26.94994862762366	32.32643475708205	19.699104652869515
18	20.58784676354029	26.761338617349185	33.72669895787465	18.92411566123587
19	20.22530456480258	29.27711727579627	31.585204755614267	18.912373403786876
20	21.05239982386614	28.47130485835902	32.691178629091446	17.7851166886834
21	21.238808160868928	28.387641274034937	33.93659180977543	16.43695875532071
22	20.93057390283282	25.410245119624246	32.76970497578159	20.889476001761338
23	18.61001027447527	27.588433876412736	33.91163951269632	19.889916336415673
24	18.97695581975635	26.574930280346397	34.60076324673418	19.84735065316307
25	18.246000293556435	30.243651842066637	32.11947746954352	19.390870394833406
26	18.91677675033025	29.67708792015265	31.238808160868924	20.16732716864817
27	18.925583443416997	29.15529135476295	31.868486716571258	20.05063848524879
28	17.713195361808307	28.49919271980038	34.09951563188023	19.68809628651108
29	18.37589901658594	27.5407309555262	33.78687802730075	20.29649200058711
30	19.26317334507559	28.915308968149127	32.88492587699985	18.93659180977543
31	21.410538676060472	28.687068838984292	31.119917804197854	18.782474680757375
32	20.62821077352121	29.815793336268896	31.075150447673565	18.480845442536328
33	19.90826361367973	28.710553353882283	31.282107735212094	20.09907529722589
34	20.47776309995597	27.963452223690005	31.704095112285337	19.854689564068693
35	20.113019227946573	28.67752825480699	30.943050051372378	20.266402465874066
36	20.818288565976808	28.676794363716425	30.079260237780716	20.425656832526055
37	20.2443857331572	29.810656098634965	31.241009834140616	18.703948334067224
38	20.10127697049758	29.15529135476295	30.76838397181858	19.975047702920886
39	20.569499486276236	28.891824453251136	30.643622486423016	19.895053574049612
40	22.61558784676354	27.42844561867019	30.531337149567005	19.424629384999268
41	20.234111257889328	27.803463965947454	30.710406575664173	21.252018200499045
42	20.899750477029208	27.822545134302068	30.1027447526787	21.174959635990017
43	20.57463672391017	28.153530016145606	29.505357404961103	21.76647585498312
44	20.129898723029502	28.67459269044474	29.45545281080288	21.740055775722883
45	19.851753999706442	28.317187729340965	29.763687068838983	22.067371202113605
46	21.26229267576692	29.004843681197713	28.82797592837223	20.904887714663147
47	19.299134008513136	27.756494936151476	31.98077205342727	20.96359900190812
48	20.213562307353587	27.322031410538678	31.133127843827975	21.33127843827976
49	19.77249376192573	26.748128577719065	31.798767062967855	21.680610597387346
50	19.275649493615145	26.83105827095259	32.17011595479231	21.72317628063995
51	20.04403346543373	26.79656538969617	30.269338030236316	22.890063114633787
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	47.0
1	123.0
2	199.0
3	1549.0
4	2899.0
5	2033.5
6	1168.0
7	1020.5
8	873.0
9	838.5
10	804.0
11	797.5
12	791.0
13	794.5
14	798.0
15	766.5
16	735.0
17	726.5
18	718.0
19	728.0
20	738.0
21	782.5
22	827.0
23	936.5
24	1046.0
25	1224.5
26	1669.0
27	1935.0
28	2242.0
29	2549.0
30	2958.0
31	3367.0
32	3876.5
33	4386.0
34	4991.0
35	5596.0
36	6024.0
37	6452.0
38	6876.0
39	7300.0
40	7974.0
41	8648.0
42	9285.0
43	9922.0
44	10361.5
45	10801.0
46	12054.5
47	13308.0
48	12452.0
49	11596.0
50	10763.0
51	9930.0
52	8692.5
53	7455.0
54	6356.5
55	5258.0
56	4632.0
57	4006.0
58	3550.0
59	3094.0
60	2781.5
61	2469.0
62	2229.5
63	1990.0
64	1615.0
65	1240.0
66	1029.0
67	818.0
68	640.5
69	463.0
70	369.0
71	275.0
72	214.5
73	154.0
74	116.0
75	63.5
76	49.0
77	37.5
78	26.0
79	21.5
80	17.0
81	15.0
82	13.0
83	8.0
84	3.0
85	5.0
86	7.0
87	5.5
88	4.0
89	2.5
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	136260.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.04902392484955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.32816620569885	42.40349332159107
2	10.802084003344696	12.324966974900924
3	5.363092558049785	9.178775869660942
4	3.2340644497330673	7.380008806693087
5	2.0093908792693123	5.7316894172904735
6	1.3893355631311508	4.7556142668428
7	0.9532385669260951	3.806693086745927
8	0.634205956133016	2.8944664611771613
9	0.38335370167878047	1.9682959048877144
>10	0.8799125233163955	6.49787171583737
>50	0.009004952723998198	0.35006605019815057
>100	0.012864218177140285	1.5404373990899751
>500	0.0	0.0
>1k	0.0012864218177140283	1.1676207250843975
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1591	1.1676207250843975	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGC	381	0.27961250550418315	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTC	362	0.2656685747835021	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCG	359	0.2634669015118156	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	280	0.20548950535740496	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	153	0.11228533685601057	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10494642595038896	0.0
2	0.0	0.0	0.0	0.4652869514164098	0.0
3	0.0	0.0	0.0	0.6715103478643769	0.0
4	0.0	0.0	0.0	0.996624100983414	0.0
5	0.0	0.0	0.0	1.964626449434904	0.0
6	0.0	0.0	0.0	2.5627476882430646	0.0
7	0.0	0.0	0.0	3.004550124761485	0.0
8	0.0	0.0	0.0	3.7450462351387053	0.0
9	0.0	0.0	0.0	4.0415382357258185	0.0
10	0.0	0.0	0.0	5.085865257595772	0.0
11	0.0	0.0	0.0	5.889476001761339	0.0
12	0.0	0.0	0.0	6.850873330397769	0.0
13	0.0	0.0	0.0	7.235432261852341	0.0
14	0.0	0.0	0.0	7.41156612358726	0.0
15	0.0	0.0	0.0	7.627330104212535	0.0
16	0.0	0.0	0.0	8.017760164391603	0.0
17	0.0	0.0	0.0	8.543226185234111	0.0
18	0.0	0.0	0.0	9.150154117129018	0.0
19	0.0	0.0	0.0	9.498018494055483	0.0
20	0.0	0.0	0.0	9.872302950242185	0.0
21	0.0	0.0	0.0	10.255394099515632	0.0
22	0.0	0.0	0.0	10.718479377660355	0.0
23	0.0	0.0	0.0	11.205049170703068	0.0
24	0.0	0.0	0.0	11.581535300161455	0.0
25	0.0	0.0	0.0	11.905915162189931	0.0
26	0.0	0.0	0.0	12.206810509320416	0.0
27	0.0	0.0	0.0	12.517246440628211	0.0
28	0.0	0.0	0.0	12.835021282841627	0.0
29	0.0	0.0	0.0	13.174078966681344	0.0
30	0.0	0.0	0.0	13.568912373403787	0.0
31	0.0	0.0	0.0	13.949067958314986	0.0
32	0.0	0.0	0.0	14.338030236312932	0.0
33	0.0	0.0	0.0	14.704975781594012	0.0
34	0.0	0.0	0.0	15.06091296051666	0.0
35	0.0	0.0	0.0	15.460883604873036	0.0
36	0.0	0.0	0.0	15.833700278878615	0.0
37	0.0	0.0	0.0	16.19257302216351	0.0
38	0.0	0.0	0.0	16.57859973579921	0.0
39	0.0	0.0	0.0	16.99691765741964	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCGG	35	1.2014061E-7	45.000004	2
CGGTTTT	70	0.0	45.000004	1
GGCGATC	35	1.2014061E-7	45.000004	8
AGACTAT	20	7.011392E-4	45.0	34
GATAATT	20	7.011392E-4	45.0	9
CTCGTCC	45	3.8016879E-10	45.0	37
GGGTATC	20	7.011392E-4	45.0	8
TAGCGGG	85	0.0	45.0	3
GACCGAT	25	3.8726685E-5	45.0	9
TCCTCGT	45	3.8016879E-10	45.0	35
CCTCGTC	45	3.8016879E-10	45.0	36
ACGGGTA	20	7.011392E-4	45.0	5
CTACGAA	20	7.011392E-4	45.0	11
TACGAAT	25	3.8726685E-5	45.0	12
GCGATGT	25	3.8726685E-5	45.0	9
GCGATGA	25	3.8726685E-5	45.0	9
TCGAGGG	25	3.8726685E-5	45.0	3
ATAGTGG	20	7.011392E-4	45.0	1
AGCTACG	20	7.011392E-4	45.0	9
CGTCCCC	45	3.8016879E-10	45.0	39
>>END_MODULE