##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936337.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237610
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10045031774757	33.0	31.0	34.0	30.0	34.0
2	32.21677117966416	33.0	31.0	34.0	30.0	34.0
3	32.0275198855267	33.0	31.0	34.0	30.0	34.0
4	35.80565632759564	37.0	35.0	37.0	35.0	37.0
5	35.901254155969866	37.0	35.0	37.0	35.0	37.0
6	35.876596102857626	37.0	35.0	37.0	35.0	37.0
7	26.710054290644333	36.0	10.0	37.0	0.0	37.0
8	31.075097849417112	35.0	19.0	37.0	17.0	37.0
9	35.921425865914735	39.0	32.0	39.0	32.0	39.0
10	36.778018601910695	38.0	35.0	39.0	33.0	39.0
11	37.16104961912377	39.0	37.0	39.0	34.0	39.0
12	37.208345608349816	39.0	37.0	39.0	34.0	39.0
13	37.18672193931232	39.0	37.0	39.0	34.0	39.0
14	38.46726147889399	40.0	38.0	41.0	34.0	41.0
15	38.502529354825135	40.0	38.0	41.0	34.0	41.0
16	38.43541517612895	40.0	38.0	41.0	34.0	41.0
17	38.311076974874794	40.0	37.0	41.0	34.0	41.0
18	38.18443668195783	40.0	37.0	41.0	33.0	41.0
19	38.057809014772104	40.0	37.0	41.0	34.0	41.0
20	38.112937165944196	40.0	37.0	41.0	34.0	41.0
21	38.086103278481545	40.0	37.0	41.0	34.0	41.0
22	38.070476831783175	40.0	37.0	41.0	34.0	41.0
23	38.046546862505785	40.0	37.0	41.0	34.0	41.0
24	37.98040065653802	40.0	36.0	41.0	34.0	41.0
25	37.88622953579395	40.0	36.0	41.0	33.0	41.0
26	37.75982492319347	40.0	36.0	41.0	33.0	41.0
27	37.69682673288161	40.0	36.0	41.0	33.0	41.0
28	37.63518370438955	40.0	36.0	41.0	33.0	41.0
29	37.590328689869956	40.0	36.0	41.0	33.0	41.0
30	37.5075249358192	40.0	36.0	41.0	33.0	41.0
31	37.40416649130929	40.0	36.0	41.0	33.0	41.0
32	37.26692479272758	40.0	35.0	41.0	32.0	41.0
33	37.126223643786034	40.0	35.0	41.0	32.0	41.0
34	36.94621017633938	39.0	35.0	41.0	31.0	41.0
35	36.851710786583055	39.0	35.0	41.0	31.0	41.0
36	36.728559404065486	39.0	35.0	41.0	31.0	41.0
37	36.665822145532594	39.0	35.0	41.0	31.0	41.0
38	36.54838601068979	39.0	35.0	41.0	30.0	41.0
39	36.46004376920163	39.0	35.0	41.0	30.0	41.0
40	36.269109044232145	39.0	35.0	41.0	30.0	41.0
41	36.17772821009217	39.0	35.0	41.0	30.0	41.0
42	36.10517654980851	39.0	35.0	40.0	29.0	41.0
43	36.07816169353142	39.0	35.0	40.0	29.0	41.0
44	35.92361432599638	39.0	35.0	40.0	29.0	41.0
45	35.84085686629351	38.0	35.0	40.0	28.0	41.0
46	35.67223601700265	38.0	35.0	40.0	28.0	41.0
47	35.51540760069021	38.0	35.0	40.0	27.0	41.0
48	35.395509448255545	38.0	34.0	40.0	26.0	41.0
49	35.232250326164724	38.0	34.0	40.0	26.0	41.0
50	35.03442616051513	38.0	34.0	40.0	26.0	41.0
51	32.9793064264972	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	25.0
10	21.0
11	18.0
12	9.0
13	10.0
14	14.0
15	20.0
16	30.0
17	52.0
18	97.0
19	177.0
20	272.0
21	419.0
22	625.0
23	828.0
24	1234.0
25	1785.0
26	2324.0
27	2756.0
28	3013.0
29	3577.0
30	4477.0
31	5594.0
32	7088.0
33	10291.0
34	15942.0
35	19650.0
36	22089.0
37	35385.0
38	46583.0
39	53179.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.11893438828332	20.19948655359623	22.29830394343672	13.383275114683727
2	32.24822187618366	25.998905769959173	24.817137325870124	16.935735027987036
3	31.386726147889398	25.92651824418164	26.310761331593792	16.37599427633517
4	28.425150456630615	24.476663440090903	28.27490425487143	18.823281848407053
5	26.492992719161652	29.03034384074744	24.620596776229956	19.85606666386095
6	25.89116619670889	35.11847144480451	24.867640250831194	14.122722107655402
7	58.70291654391651	28.596439543790243	8.420521021842516	4.280122890450739
8	79.2218340978915	7.0922940953663565	9.6603678296368	4.025503977105341
9	72.22044526745506	8.924708556037205	11.281090863179159	7.573755313328563
10	40.150667059467196	27.88519001725517	17.950843819704556	14.013299103573083
11	30.68431463322251	25.233786456798956	25.924834813349605	18.15706409662893
12	30.244097470645176	22.91317705483776	28.31488573713228	18.52783973738479
13	24.848701653970792	23.331509616598627	30.273978367913806	21.54581036151677
14	20.25377719792938	27.525777534615546	29.068221034468245	23.152224232986825
15	20.07028323723749	26.84777576701317	33.35255250199907	19.729388493750264
16	23.29279070746181	26.05572156054038	30.365304490551743	20.286183241446068
17	22.392155212322713	26.437018643996463	28.64904675729136	22.52177938638946
18	22.59711291612306	28.14191321914061	28.05563738899878	21.205336475737553
19	24.589032448129288	28.105719456251844	26.3823071419553	20.922940953663566
20	26.081393880728925	26.227852363116032	28.929337990825303	18.76141576532974
21	25.490509658684402	27.709271495307437	27.709271495307437	19.09094735070073
22	24.021295400025252	24.836917638146545	28.4811245317958	22.660662430032406
23	24.240983123605908	26.717730735238415	29.006775809098944	20.034510332056733
24	24.078111190606457	24.51748663776777	29.042969571987708	22.36143259963806
25	23.06510668742898	27.593114767896974	26.843988047641094	22.497790497032955
26	21.33285636126426	27.952106392828586	28.138125499768528	22.57691174613863
27	22.369008038382223	27.85194225832246	28.264803669879214	21.5142460334161
28	19.97601111064349	28.234081057194558	30.09763898825807	21.692268843903875
29	22.358486595682	26.225327216867978	29.17217288834645	22.244013299103575
30	21.766339800513446	25.65253987626783	31.21880392239384	21.36231640082488
31	23.469130087117545	25.849922141324015	28.692395101216277	21.988552670342155
32	23.892092083666512	27.072513783089935	27.686124321366947	21.349269811876603
33	22.646774125668113	26.814107150372458	27.706325491351375	22.832793232608058
34	22.653507848996256	26.841462901393037	29.631749505492195	20.873279744118513
35	22.307141955304914	26.894490972602163	28.581288666301923	22.217078405791003
36	22.04578931863137	29.64521695214848	28.208408737006017	20.10058499221413
37	22.3349185640335	28.762257480745763	28.546778334245193	20.35604562097555
38	21.646395353730906	28.107823744791887	26.877235806573797	23.36854509490341
39	23.974580194436264	26.00606035099533	26.892807541770125	23.12655191279828
40	22.867724422372795	26.34948024073061	28.968056899962125	21.814738436934473
41	20.288287529986114	28.159168385168975	29.306847354909305	22.24569672993561
42	20.595513656832626	27.976936997601108	29.314001935945456	22.113547409620807
43	22.169521484785996	26.734985901266782	27.65245570472623	23.443036909220993
44	21.911956567484534	26.210597197087665	28.17263583182526	23.70481040360254
45	22.07735364673204	25.72534825975338	27.677707167206766	24.519590926307817
46	22.41656495938723	28.264382812171206	27.02032742729683	22.298724801144733
47	21.43975421909852	25.943773410210007	30.433062581541183	22.18340978915029
48	21.35221581583267	26.33769622490636	29.458356129792516	22.85173182946846
49	20.993224190901056	26.339379655738394	29.631328647784184	23.036067505576366
50	19.67173098775304	26.252262110180546	29.856066663860947	24.21994023820546
51	20.205378561508354	25.612137536298977	28.85232103025967	25.330162871933
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	89.0
1	325.0
2	561.0
3	1721.0
4	2881.0
5	1929.5
6	978.0
7	831.0
8	684.0
9	655.5
10	627.0
11	626.5
12	626.0
13	607.5
14	589.0
15	601.5
16	614.0
17	651.5
18	689.0
19	682.0
20	675.0
21	789.5
22	904.0
23	1061.0
24	1218.0
25	1549.0
26	2276.0
27	2672.0
28	3230.5
29	3789.0
30	4586.0
31	5383.0
32	6310.5
33	7238.0
34	7594.0
35	7950.0
36	8331.0
37	8712.0
38	9279.0
39	9846.0
40	10815.0
41	11784.0
42	12953.0
43	14122.0
44	17352.5
45	20583.0
46	18759.5
47	16936.0
48	16608.0
49	16280.0
50	15457.0
51	14634.0
52	13623.0
53	12612.0
54	12228.0
55	11844.0
56	11818.5
57	11793.0
58	11749.0
59	11705.0
60	11383.5
61	11062.0
62	10013.5
63	8965.0
64	7474.0
65	5983.0
66	4991.0
67	3999.0
68	3292.0
69	2585.0
70	2088.5
71	1592.0
72	1328.0
73	1064.0
74	870.0
75	507.0
76	338.0
77	271.0
78	204.0
79	155.5
80	107.0
81	84.5
82	62.0
83	42.0
84	22.0
85	24.5
86	27.0
87	20.0
88	13.0
89	8.0
90	3.0
91	3.5
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	237610.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.58664216779892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.80960671850531	27.600235303567626
2	11.403930797991585	9.713102404008184
3	6.756548236220581	8.632161060761966
4	4.402990306545924	7.500342906126341
5	3.1809812422833774	6.773365495380135
6	2.3989854605648873	6.129884122489749
7	1.8699886530539767	5.57455763378178
8	1.4089914498956535	4.80033717553555
9	1.0899933856382893	4.1777242451500936
>10	2.6229840830526294	14.54452763960425
>50	0.02599984222621722	0.7610186774799468
>100	0.024999848294439637	1.8840216167718435
>500	9.999939317775854E-4	0.38625850054522204
>1k	0.0029999817953327563	1.5224632187973195
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1462	0.6152939691090442	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	1091	0.4591557594377341	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	1022	0.43011657758511845	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGC	907	0.3817179411640924	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	315	0.1325701780228105	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTT	291	0.12246959303059636	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	282	0.11868187365851605	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT	264	0.11110643491435546	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	240	0.10100584992214132	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06607466015740078	0.0
2	0.0	0.0	0.0	0.33331930474306637	0.0
3	0.0	0.0	0.0	0.44484659736543075	0.0
4	0.0	0.0	0.0	0.6780017676023736	0.0
5	0.0	0.0	0.0	1.6405033458187788	0.0
6	0.0	0.0	0.0	2.058835907579647	0.0
7	0.0	0.0	0.0	2.3816337696224905	0.0
8	0.0	0.0	0.0	2.925802786078027	0.0
9	0.0	0.0	0.0	3.1273936282143007	0.0
10	0.0	0.0	0.0	4.121038676823366	0.0
11	0.0	0.0	0.0	4.6273304995580995	0.0
12	0.0	0.0	0.0	5.522915702201086	0.0
13	0.0	0.0	0.0	5.7186145364252345	0.0
14	0.0	0.0	0.0	5.83350869071167	0.0
15	0.0	0.0	0.0	6.039308109928033	0.0
16	0.0	0.0	0.0	6.329279070746181	0.0
17	0.0	0.0	0.0	6.656285509869114	0.0
18	0.0	0.0	0.0	6.990867387736206	0.0
19	0.0	0.0	0.0	7.213921972980935	0.0
20	0.0	0.0	0.0	7.441185135305753	0.0
21	0.0	0.0	0.0	7.74672783132023	0.0
22	0.0	0.0	0.0	8.045115946298557	0.0
23	0.0	0.0	0.0	8.339295484196793	0.0
24	0.0	0.0	0.0	8.562770927149531	0.0
25	0.0	0.0	0.0	8.78961323176634	0.0
26	0.0	0.0	0.0	9.012667817011069	0.0
27	0.0	0.0	0.0	9.219729809351458	0.0
28	0.0	0.0	0.0	9.460460418332561	0.0
29	0.0	0.0	0.0	9.673414418585077	0.0
30	0.0	0.0	0.0	9.934346197550608	0.0
31	0.0	0.0	0.0	10.162871932999453	0.0
32	0.0	0.0	0.0	10.412861411556753	0.0
33	0.0	0.0	0.0	10.672951475106267	0.0
34	0.0	0.0	0.0	10.908631791591263	0.0
35	0.0	0.0	0.0	11.161988131812635	0.0
36	0.0	0.0	0.0	11.404823029333782	0.0
37	0.0	0.0	0.0	11.672909389335466	0.0
38	0.0	0.0	0.0	11.946887757249273	0.0
39	0.0	0.0	0.0	12.214974117250957	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.02198E-4	45.000004	6
GCGAAAA	20	7.02198E-4	45.000004	1
ATTACGG	20	7.02198E-4	45.000004	2
CGATTGA	20	7.02198E-4	45.000004	10
TATGCGG	20	7.02198E-4	45.000004	2
CCCACAT	20	7.02198E-4	45.000004	45
CGAATAT	45	3.8198777E-10	45.000004	14
GCTACGA	45	3.8198777E-10	45.000004	10
CAATACA	20	7.02198E-4	45.000004	38
TGCGTAG	20	7.02198E-4	45.000004	1
ACAACCT	25	3.8814378E-5	45.0	37
AATCCCC	25	3.8814378E-5	45.0	37
ATATCAC	50	2.1827873E-11	45.0	30
ATCTATC	70	0.0	41.785713	3
GCATATC	55	6.002665E-11	40.909092	28
GACCCAC	50	1.0732037E-9	40.5	12
TACGAAT	50	1.0732037E-9	40.5	12
TCTATCT	75	0.0	39.0	4
CTATCTC	110	0.0	38.863636	5
ACATACG	70	0.0	38.571426	17
>>END_MODULE