##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936334.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197960
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01601838755304	33.0	31.0	34.0	30.0	34.0
2	32.150015154576685	33.0	31.0	34.0	30.0	34.0
3	32.02229743382502	33.0	31.0	34.0	30.0	34.0
4	35.77317134774702	37.0	35.0	37.0	35.0	37.0
5	35.855652657102446	37.0	35.0	37.0	35.0	37.0
6	35.8372297433825	37.0	35.0	37.0	35.0	37.0
7	26.78132451000202	35.0	10.0	37.0	0.0	37.0
8	31.135193978581533	35.0	19.0	37.0	17.0	37.0
9	35.91261365932512	39.0	32.0	39.0	32.0	39.0
10	36.71067892503536	38.0	35.0	39.0	33.0	39.0
11	37.068261264902	39.0	37.0	39.0	34.0	39.0
12	37.0920135380885	39.0	37.0	39.0	34.0	39.0
13	37.04418569407961	39.0	37.0	39.0	33.0	39.0
14	38.2189937361083	40.0	37.0	41.0	33.0	41.0
15	38.28620933521923	40.0	37.0	41.0	34.0	41.0
16	38.2048999797939	40.0	37.0	41.0	33.0	41.0
17	38.08030915336432	40.0	37.0	41.0	33.0	41.0
18	38.01776621539705	40.0	37.0	41.0	33.0	41.0
19	37.926581127500505	40.0	37.0	41.0	33.0	41.0
20	37.96866538694686	40.0	36.0	41.0	33.0	41.0
21	37.93880581935745	40.0	36.0	41.0	33.0	41.0
22	37.93776015356638	40.0	36.0	41.0	33.0	41.0
23	37.90466255809255	40.0	36.0	41.0	33.0	41.0
24	37.83707314609012	40.0	36.0	41.0	33.0	41.0
25	37.75334916144676	40.0	36.0	41.0	33.0	41.0
26	37.61746312386341	40.0	36.0	41.0	33.0	41.0
27	37.49248332996565	39.0	36.0	41.0	33.0	41.0
28	37.432910689028084	39.0	36.0	41.0	33.0	41.0
29	37.385436451808445	39.0	36.0	41.0	33.0	41.0
30	37.310926449787836	39.0	35.0	41.0	32.0	41.0
31	37.16706910486967	39.0	35.0	41.0	32.0	41.0
32	37.04226106284098	39.0	35.0	41.0	31.0	41.0
33	36.948196605374825	39.0	35.0	41.0	31.0	41.0
34	36.76452313598707	39.0	35.0	41.0	31.0	41.0
35	36.70941604364518	39.0	35.0	41.0	31.0	41.0
36	36.58163770458678	39.0	35.0	41.0	30.0	41.0
37	36.51402808648212	39.0	35.0	41.0	30.0	41.0
38	36.40597090321277	39.0	35.0	41.0	30.0	41.0
39	36.33728531016367	39.0	35.0	41.0	30.0	41.0
40	36.168422913719944	39.0	35.0	40.0	30.0	41.0
41	36.101505354617096	39.0	35.0	40.0	29.0	41.0
42	36.045074762578295	39.0	35.0	40.0	29.0	41.0
43	36.030657708628006	38.0	35.0	40.0	29.0	41.0
44	35.854187714689836	38.0	35.0	40.0	29.0	41.0
45	35.75865831481107	38.0	35.0	40.0	28.0	41.0
46	35.60666801374015	38.0	35.0	40.0	28.0	41.0
47	35.478743180440496	38.0	34.0	40.0	27.0	41.0
48	35.38830571832693	38.0	34.0	40.0	27.0	41.0
49	35.221236613457265	38.0	34.0	40.0	26.0	41.0
50	35.0172964235199	38.0	34.0	40.0	26.0	41.0
51	32.7873459284704	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	15.0
10	11.0
11	12.0
12	7.0
13	9.0
14	4.0
15	14.0
16	15.0
17	31.0
18	65.0
19	138.0
20	218.0
21	365.0
22	552.0
23	721.0
24	1019.0
25	1421.0
26	1926.0
27	2284.0
28	2659.0
29	3131.0
30	3884.0
31	4904.0
32	6461.0
33	9129.0
34	14588.0
35	17417.0
36	18772.0
37	29481.0
38	37690.0
39	41003.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.47736916548798	21.46342695494039	24.880783996767022	13.178419882804606
2	31.531622550010102	25.845120226308342	25.28389573651243	17.339361487169125
3	32.53435037381289	24.568094564558496	26.60335421297232	16.294200848656295
4	29.55799151343706	24.7696504344312	26.64275611234593	19.029601939785813
5	25.316730652657103	29.878763386542733	24.763588603758336	20.040917357041828
6	26.8286522529804	34.59486765003031	25.00303091533643	13.573449181652858
7	60.7541927662154	28.30975954738331	6.749848454233179	4.186199232168115
8	81.93271367953122	6.194685795110123	7.869771671044655	4.002828854314003
9	74.8918973530006	8.454738330975955	10.296019397858153	6.357344918165286
10	41.913517882400484	28.07688421903415	16.537684380682965	13.4719135178824
11	32.09890887047889	25.20357648009699	24.982319660537485	17.715194988886644
12	31.234087694483737	22.174176601333603	27.961204283693675	18.63053142048899
13	25.63952313598707	23.826025459688825	29.079106890280865	21.45534451404324
14	19.890887047888462	27.775308143059203	29.051323499696906	23.282481309355425
15	20.612750050515256	26.345221256819563	33.50171751869064	19.54031117397454
16	23.82451000202061	25.418771468983632	30.68650232370176	20.070216205294
17	22.370680945645585	25.965346534653467	28.20064659527177	23.463325924429178
18	23.2001414427157	27.01252778339058	28.476459890887046	21.310870883006668
19	25.63699737320671	28.172358052131745	25.943624974742374	20.247019599919174
20	27.02414629218024	26.92867245908264	27.174176601333606	18.873004647403516
21	25.701151747827844	27.610628409779753	27.027177207516672	19.661042634875734
22	23.989694887856132	25.530915336431605	27.41614467569206	23.063245100020204
23	23.62649020004041	26.53212770256618	28.219337239846432	21.622044857546978
24	24.505455647605576	24.478177409577693	29.04273590624368	21.973631036573046
25	23.153667407557084	26.414427157001413	27.212063043039	23.219842392402505
26	21.50434431198222	27.5990099009901	27.864720145483936	23.031925641543747
27	21.789755506162862	27.914225095978985	27.925338452212568	22.370680945645585
28	20.476358860375836	28.192564154374622	29.20387957163063	22.127197413618912
29	22.42675287936957	27.08173368357244	28.624469589816126	21.867043847241867
30	22.657607597494444	26.182056981208323	28.166801374014955	22.993534047282278
31	24.557486360880986	27.13477470195999	26.116387148918975	22.19135178824005
32	24.575671852899575	28.68054152354011	26.059304910082847	20.68448171347747
33	23.94271570014144	27.15043443119822	25.968882602545968	22.937967266114367
34	22.299454435239443	27.007476257829865	28.35623358254193	22.336835724388767
35	21.552333804809052	26.511921600323298	28.392604566579106	23.54314002828854
36	22.027177207516672	28.819458476459893	26.846837744998993	22.30652657102445
37	22.525762780359667	29.548393614871692	26.871590220246517	21.054253384522127
38	22.113053142048898	28.37441907456052	26.0007072135785	23.511820569812084
39	23.530511214386745	27.11406344716104	26.319963629015962	23.03546170943625
40	22.402000404122045	26.489189735300062	28.50323297635886	22.605576884219033
41	19.358456253788646	27.083249141240657	28.45726409375631	25.10103051121439
42	20.875934532228733	26.722065063649218	27.89856536674076	24.503435037381287
43	22.1868054152354	25.321277025661747	27.329258436047688	25.162659123055164
44	21.50484946453829	26.514447363103656	27.102950090927457	24.877753081430594
45	21.7710648615882	26.335118205698123	26.224489795918366	25.66932713679531
46	22.043342089310972	28.189028086482114	26.26187108506769	23.50575873913922
47	20.900181854920184	25.72388361285108	29.571630632451	23.804303899777732
48	21.429581733683573	24.9136189129117	29.591331582137805	24.06546777126692
49	21.387654071529603	25.086886239644375	29.03919983835118	24.48625985047484
50	19.274095776924632	25.81430592038796	29.116993331986258	25.79460497070115
51	18.97656092139826	25.72691452818751	28.53101636694282	26.76550818347141
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	89.0
1	272.0
2	455.0
3	836.0
4	1217.0
5	850.5
6	484.0
7	443.5
8	403.0
9	385.0
10	367.0
11	380.5
12	394.0
13	420.0
14	446.0
15	466.5
16	487.0
17	471.0
18	455.0
19	585.0
20	715.0
21	802.0
22	889.0
23	950.0
24	1011.0
25	1269.0
26	1845.0
27	2163.0
28	2658.0
29	3153.0
30	3516.0
31	3879.0
32	4495.5
33	5112.0
34	5628.0
35	6144.0
36	6517.0
37	6890.0
38	7655.5
39	8421.0
40	9135.0
41	9849.0
42	10559.5
43	11270.0
44	12271.0
45	13272.0
46	14740.5
47	16209.0
48	15897.5
49	15586.0
50	14920.5
51	14255.0
52	13022.5
53	11790.0
54	10934.0
55	10078.0
56	9983.5
57	9889.0
58	9749.5
59	9610.0
60	9475.5
61	9341.0
62	8525.0
63	7709.0
64	6540.5
65	5372.0
66	4305.5
67	3239.0
68	2670.0
69	2101.0
70	1790.0
71	1479.0
72	1204.5
73	930.0
74	735.0
75	405.0
76	270.0
77	234.0
78	198.0
79	173.5
80	149.0
81	102.5
82	56.0
83	45.5
84	35.0
85	24.0
86	13.0
87	9.5
88	6.0
89	4.0
90	2.0
91	4.0
92	6.0
93	3.5
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	197960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.530208122853104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.13059261275978	30.78399676702364
2	9.941918504401489	8.854314002828854
3	5.338506216535076	7.131743786623561
4	3.6788728559760413	6.5528389573651245
5	2.742989381976586	6.1072944029096785
6	2.027180324893366	5.416245706203274
7	1.476994282602777	4.603960396039604
8	1.289817587802886	4.594867650030309
9	0.9880660677012434	3.9598908870478886
>10	3.340820401125329	19.646393210749647
>50	0.030628913694527637	0.9633259244291776
>100	0.012478446319992741	1.1244695898161243
>500	0.001134404210908431	0.2606587189331178
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	516	0.2606587189331178	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTC	356	0.1798343099616084	RNA PCR Primer, Index 41 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCG	352	0.17781369973732067	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGC	326	0.1646797332794504	No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	232	0.11719539300868863	Illumina PCR Primer Index 11 (96% over 25bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	217	0.10961810466760963	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	203	0.10254596888260256	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09143261264902	0.0
2	0.0	0.0	0.0	0.4480703172358052	0.0
3	0.0	0.0	0.0	0.5758739139220045	0.0
4	0.0	0.0	0.0	0.793089513032936	0.0
5	0.0	0.0	0.0	1.5942614669630228	0.0
6	0.0	0.0	0.0	1.9998989694887856	0.0
7	0.0	0.0	0.0	2.3898767427763183	0.0
8	0.0	0.0	0.0	3.1228531016366943	0.0
9	0.0	0.0	0.0	3.406748838149121	0.0
10	0.0	0.0	0.0	4.272075166700343	0.0
11	0.0	0.0	0.0	5.150030309153364	0.0
12	0.0	0.0	0.0	6.078500707213578	0.0
13	0.0	0.0	0.0	6.396241664982825	0.0
14	0.0	0.0	0.0	6.561931703374419	0.0
15	0.0	0.0	0.0	6.816023439078601	0.0
16	0.0	0.0	0.0	7.268640129319055	0.0
17	0.0	0.0	0.0	7.825823398666397	0.0
18	0.0	0.0	0.0	8.417357041826632	0.0
19	0.0	0.0	0.0	8.79824206910487	0.0
20	0.0	0.0	0.0	9.220549605981006	0.0
21	0.0	0.0	0.0	9.688826025459688	0.0
22	0.0	0.0	0.0	10.181349767629824	0.0
23	0.0	0.0	0.0	10.658213780561729	0.0
24	0.0	0.0	0.0	11.035562739947464	0.0
25	0.0	0.0	0.0	11.374520105071731	0.0
26	0.0	0.0	0.0	11.681147706607396	0.0
27	0.0	0.0	0.0	11.988280460699132	0.0
28	0.0	0.0	0.0	12.333299656496262	0.0
29	0.0	0.0	0.0	12.658617902606586	0.0
30	0.0	0.0	0.0	13.029905031319458	0.0
31	0.0	0.0	0.0	13.402707617700546	0.0
32	0.0	0.0	0.0	13.775510204081632	0.0
33	0.0	0.0	0.0	14.136189129116993	0.0
34	0.0	0.0	0.0	14.510507173166296	0.0
35	0.0	0.0	0.0	14.88785613255203	0.0
36	0.0	0.0	0.0	15.262174176601334	0.0
37	0.0	0.0	0.0	15.627399474641342	0.0
38	0.0	0.0	0.0	15.985552636896342	0.0
39	0.0	0.0	0.0	16.36593251161851	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATATG	20	7.0191285E-4	45.0	1
CGTGGCG	20	7.0191285E-4	45.0	27
AAATCGA	20	7.0191285E-4	45.0	34
TATGGGT	20	7.0191285E-4	45.0	4
CAACCGT	20	7.0191285E-4	45.0	40
TCAGACG	25	3.879075E-5	45.0	22
AATCTCG	20	7.0191285E-4	45.0	18
CTATTGG	20	7.0191285E-4	45.0	2
CTCGAAC	20	7.0191285E-4	45.0	13
ATAACGG	20	7.0191285E-4	45.0	2
ACCCGCT	20	7.0191285E-4	45.0	34
CGGTAGT	25	3.879075E-5	45.0	12
GTAACGG	25	3.879075E-5	45.0	2
CACCCAT	45	3.8198777E-10	45.0	31
TAACGGG	60	3.6379788E-12	41.250004	3
CTATCTC	55	6.002665E-11	40.909092	5
ACGGGCT	50	1.0713848E-9	40.5	5
AACGGGA	45	1.9144863E-8	40.0	4
TAGGGCG	45	1.9144863E-8	40.0	5
CATACGA	45	1.9144863E-8	40.0	18
>>END_MODULE