##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936333.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	226765
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89658016007761	33.0	31.0	34.0	30.0	34.0
2	32.007889224527595	33.0	31.0	34.0	30.0	34.0
3	31.48985513637466	33.0	31.0	34.0	28.0	34.0
4	35.455489162789675	37.0	35.0	37.0	33.0	37.0
5	35.699583269022995	37.0	35.0	37.0	35.0	37.0
6	35.73606156152845	37.0	35.0	37.0	35.0	37.0
7	26.980936211496484	35.0	16.0	37.0	0.0	37.0
8	31.27577889004035	36.0	23.0	37.0	17.0	37.0
9	36.054898242674135	39.0	32.0	39.0	32.0	39.0
10	36.75338345864662	38.0	35.0	39.0	33.0	39.0
11	37.05438229003594	39.0	37.0	39.0	34.0	39.0
12	37.17224439397614	39.0	37.0	39.0	34.0	39.0
13	37.205719577536215	39.0	37.0	39.0	34.0	39.0
14	38.47558926642118	40.0	38.0	41.0	34.0	41.0
15	38.51706833065067	40.0	38.0	41.0	34.0	41.0
16	38.43351487222455	40.0	38.0	41.0	34.0	41.0
17	38.30849998897537	40.0	38.0	41.0	34.0	41.0
18	38.130875576036864	40.0	37.0	41.0	33.0	41.0
19	37.94183405728397	40.0	37.0	41.0	34.0	41.0
20	37.909805305051485	40.0	36.0	41.0	34.0	41.0
21	37.857478005865104	40.0	36.0	41.0	33.0	41.0
22	37.85610213216325	40.0	36.0	41.0	33.0	41.0
23	37.79265318722025	40.0	36.0	41.0	33.0	41.0
24	37.731501774965274	40.0	35.0	41.0	33.0	41.0
25	37.634066103675615	39.0	35.0	41.0	33.0	41.0
26	37.53069036226931	39.0	35.0	41.0	33.0	41.0
27	37.437069212621	39.0	35.0	41.0	33.0	41.0
28	37.36935153132097	39.0	35.0	41.0	33.0	41.0
29	37.34619981037638	39.0	35.0	41.0	33.0	41.0
30	37.25637113311137	39.0	35.0	41.0	33.0	41.0
31	37.10222917998809	39.0	35.0	41.0	32.0	41.0
32	36.87517033051838	39.0	35.0	41.0	31.0	41.0
33	36.61662073071241	39.0	35.0	41.0	30.0	41.0
34	36.315692456948824	39.0	35.0	41.0	30.0	41.0
35	36.064877736864155	39.0	35.0	41.0	28.0	41.0
36	35.83994884572134	39.0	35.0	41.0	27.0	41.0
37	35.754948955967635	39.0	35.0	41.0	26.0	41.0
38	35.60284435428748	39.0	35.0	41.0	25.0	41.0
39	35.5448592154874	39.0	35.0	41.0	25.0	41.0
40	35.37384076025842	39.0	35.0	40.0	24.0	41.0
41	35.298308821908144	39.0	35.0	40.0	24.0	41.0
42	35.24169514695831	39.0	35.0	40.0	24.0	41.0
43	35.21024408528653	39.0	35.0	40.0	24.0	41.0
44	35.04014728904372	38.0	34.0	40.0	23.0	41.0
45	34.95354221330452	38.0	34.0	40.0	23.0	41.0
46	34.83731616431107	38.0	34.0	40.0	23.0	41.0
47	34.711189998456554	38.0	34.0	40.0	23.0	41.0
48	34.63568010936432	38.0	34.0	40.0	22.0	41.0
49	34.49336537825502	38.0	34.0	40.0	21.0	41.0
50	34.268114567944785	38.0	34.0	40.0	20.0	41.0
51	32.37943245209799	36.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	21.0
10	7.0
11	12.0
12	16.0
13	4.0
14	8.0
15	14.0
16	28.0
17	51.0
18	95.0
19	158.0
20	263.0
21	480.0
22	803.0
23	1208.0
24	1965.0
25	2969.0
26	3825.0
27	4116.0
28	4180.0
29	4249.0
30	4558.0
31	5562.0
32	7035.0
33	9317.0
34	14410.0
35	17960.0
36	19941.0
37	32315.0
38	42127.0
39	49049.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.432738738341456	19.816550173086675	24.203911538376733	17.546799550195136
2	35.12667298745397	24.259475668643752	24.73221176107424	15.881639582828038
3	30.317729808391945	23.64165545829383	30.82750865433378	15.213106078980443
4	25.783079399378213	24.303133199567835	32.20646925230966	17.707318148744296
5	23.97856812118272	28.815293365378253	29.680947236125505	17.525191277313517
6	24.43895662910943	32.628933036403325	29.6827111767689	13.249399157718342
7	58.79566952572046	26.873194716997773	10.978325579344254	3.3528101779375126
8	80.23813198685865	4.456596035543404	12.32641721606068	2.9788547615372742
9	74.22177143739113	7.270522346923027	13.713315546931845	4.794390668753996
10	36.79359689546447	32.97466540250921	19.672348025488944	10.559389676537384
11	25.98901946949485	24.8578925319163	32.98613101669129	16.16695698189756
12	25.40295019072608	22.167883050735345	34.31129142504355	18.11787533349503
13	21.764822613719048	23.555663351928207	36.148876590302734	18.530637444050008
14	18.333958062311204	26.46351950256874	34.86825568319626	20.3342667519238
15	18.053491500011024	27.294776530769738	37.11816197384958	17.533569995369653
16	21.829647432363902	27.16115802703239	34.06742663109386	16.941767909509846
17	21.580490816484023	26.440147289043725	32.867506008422815	19.111855886049433
18	22.154653495909862	26.440588274204575	33.73227790884837	17.6724803210372
19	21.741009415033183	28.68564372808855	31.882786144246246	17.69056071263202
20	23.98474191343461	26.98211805172756	32.82737635878553	16.2057636760523
21	23.188763698101557	27.01430996846956	32.92130619804644	16.875620135382444
22	22.17626176879148	24.648424580512867	32.91998324256389	20.255330408131766
23	21.136859744669593	26.59669702114524	33.696558110819566	18.569885123365598
24	21.174343483341783	25.188190417392452	34.1776729213062	19.45979317795956
25	19.988975370978768	27.637862985910523	32.036248980221814	20.336912662888892
26	20.13538244438075	27.52011994796375	31.91938791259674	20.42510969505876
27	20.034837827707097	27.75957489030494	32.583952549996695	19.621634731991268
28	18.15006725023703	28.051066081626352	34.02597402597402	19.77289264216259
29	20.315304390007277	26.078539457147265	33.20485965647256	20.401296496372897
30	20.931360659713803	26.339161687209224	32.936299693515316	19.79317795956166
31	21.541684122329283	26.50849998897537	31.580270323903605	20.369545564791743
32	21.898000132295547	26.971975393028025	31.922033823561836	19.20799065111459
33	21.207858355566337	27.446916411262762	31.380504046038848	19.964721187132053
34	19.49683593147091	27.902895067580975	32.55528851454149	20.044980486406633
35	19.88710779882257	28.395034507088834	31.589090027120587	20.128767666968006
36	19.925473507816463	30.202632681410268	30.45575816373779	19.416135647035475
37	20.4656803298569	29.82382642824069	31.096068617291028	18.61442462461138
38	19.911361982669284	29.75547372830904	30.121050426653145	20.21211386236853
39	21.05792340087756	28.60450245849227	30.857495645271538	19.480078495358633
40	20.849778404956673	28.436487112208674	31.062994730227327	19.650739752607326
41	18.98882102617247	28.614645117191806	31.2826053403303	21.113928516305428
42	20.171102242409546	27.796176658655437	30.544837166229357	21.487883932705664
43	21.074680836989835	27.18100235927061	30.513968204969906	21.230348598769652
44	20.3003108945384	27.87996383921681	29.683593147090598	22.13613211915419
45	20.683526999316474	27.385178488743854	29.479417017617358	22.451877494322318
46	20.39115383767336	29.339624721628116	28.80029986990938	21.468921570789142
47	19.743346636385688	27.7860339999559	31.388882764094987	21.081736599563424
48	20.43216545763235	26.875399642802016	31.227482195224134	21.4649527043415
49	20.064383833484005	26.772209115163275	31.689634643794236	21.473772407558485
50	18.886953454016272	26.974621303993118	32.20558728198796	21.932837960002647
51	19.245474389786786	26.806164972548675	30.220272087844247	23.7280885498203
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	224.5
2	375.0
3	3123.0
4	5871.0
5	4087.5
6	2304.0
7	1944.5
8	1585.0
9	1561.5
10	1538.0
11	1529.5
12	1521.0
13	1479.5
14	1438.0
15	1438.0
16	1438.0
17	1340.0
18	1242.0
19	1291.0
20	1340.0
21	1378.5
22	1417.0
23	1576.0
24	1735.0
25	2051.0
26	2790.0
27	3213.0
28	3815.0
29	4417.0
30	5010.0
31	5603.0
32	6398.0
33	7193.0
34	7952.5
35	8712.0
36	9289.5
37	9867.0
38	10422.0
39	10977.0
40	11895.5
41	12814.0
42	14024.0
43	15234.0
44	17120.5
45	19007.0
46	18326.0
47	17645.0
48	16914.0
49	16183.0
50	14622.0
51	13061.0
52	12013.0
53	10965.0
54	9976.0
55	8987.0
56	8604.0
57	8221.0
58	7983.5
59	7746.0
60	7426.0
61	7106.0
62	6302.5
63	5499.0
64	4603.5
65	3708.0
66	3002.0
67	2296.0
68	1903.0
69	1510.0
70	1251.0
71	992.0
72	814.5
73	637.0
74	496.5
75	305.0
76	254.0
77	200.0
78	146.0
79	108.5
80	71.0
81	57.0
82	43.0
83	33.5
84	24.0
85	17.0
86	10.0
87	10.0
88	10.0
89	7.0
90	4.0
91	4.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	226765.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.59836929472822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.65972443907975	31.728941809361388
2	12.526282485004387	11.924612392226495
3	6.854203594855216	9.787467417875169
4	4.179318174684731	7.957149195152533
5	2.877189691209858	6.847476872659593
6	1.9830878955763291	5.663504999852853
7	1.4218397796267692	4.737407843602696
8	1.0128919884535157	3.8569605537665628
9	0.7094214341410972	3.0390573067059288
>10	1.7461910975048547	10.765278341351218
>50	0.015919690593845546	0.5773117807870848
>100	0.010944787283268814	1.1011073751681477
>500	0.0019899613242306932	0.5692606730976832
>1k	9.949806621153466E-4	1.444463438392624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3050	1.3450047405904793	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTC	681	0.30031089453839876	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	521	0.2297532688025048	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG	426	0.18785967852181773	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGC	388	0.17110224240954292	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	341	0.15037593984962408	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.045421471567481754	0.0
2	0.0	0.0	0.0	0.2676779926355478	0.0
3	0.0	0.0	0.0	0.41540802152007583	0.0
4	0.0	0.0	0.0	0.6473662161268273	0.0
5	0.0	0.0	0.0	1.3604392212202059	0.0
6	0.0	0.0	0.0	1.802306352391242	0.0
7	0.0	0.0	0.0	2.1824355610433708	0.0
8	0.0	0.0	0.0	2.8536149758560625	0.0
9	0.0	0.0	0.0	3.2130178819482724	0.0
10	0.0	0.0	0.0	4.053094613366261	0.0
11	0.0	0.0	0.0	4.6969329482062925	0.0
12	0.0	0.0	0.0	5.435583092628933	0.0
13	0.0	0.0	0.0	5.733248076202236	0.0
14	0.0	0.0	0.0	5.8686305205829825	0.0
15	0.0	0.0	0.0	6.060900050713293	0.0
16	0.0	0.0	0.0	6.357242078804048	0.0
17	0.0	0.0	0.0	6.738253257777876	0.0
18	0.0	0.0	0.0	7.151456353493705	0.0
19	0.0	0.0	0.0	7.43412784159813	0.0
20	0.0	0.0	0.0	7.695191056820938	0.0
21	0.0	0.0	0.0	8.022843031331996	0.0
22	0.0	0.0	0.0	8.36592948647278	0.0
23	0.0	0.0	0.0	8.736357021586224	0.0
24	0.0	0.0	0.0	9.037108901285471	0.0
25	0.0	0.0	0.0	9.294644235221485	0.0
26	0.0	0.0	0.0	9.559235331731088	0.0
27	0.0	0.0	0.0	9.830441205653429	0.0
28	0.0	0.0	0.0	10.106056931184266	0.0
29	0.0	0.0	0.0	10.401957974114172	0.0
30	0.0	0.0	0.0	10.74195753312901	0.0
31	0.0	0.0	0.0	11.036094635415518	0.0
32	4.409851608493374E-4	0.0	0.0	11.35492690670959	0.0
33	4.409851608493374E-4	0.0	0.0	11.658324697373933	0.0
34	4.409851608493374E-4	0.0	0.0	11.930412541617974	0.0
35	4.409851608493374E-4	0.0	0.0	12.25189072387714	0.0
36	4.409851608493374E-4	0.0	0.0	12.579983683549049	0.0
37	4.409851608493374E-4	0.0	0.0	12.890437236786982	0.0
38	4.409851608493374E-4	0.0	0.0	13.245430291270699	0.0
39	4.409851608493374E-4	0.0	0.0	13.619385707670936	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGGCGG	20	7.021299E-4	45.0	2
CGGTTTA	20	7.021299E-4	45.0	1
TAGCGGA	20	7.021299E-4	45.0	1
CGACCCC	20	7.021299E-4	45.0	20
ACGGGCT	25	3.880872E-5	45.0	5
TATTGCG	20	7.021299E-4	45.0	1
TACGAAT	25	3.880872E-5	45.0	12
ACTACTA	20	7.021299E-4	45.0	23
AGCTCGG	20	7.021299E-4	45.0	2
TAGTGCG	20	7.021299E-4	45.0	1
TAAGCGG	25	3.880872E-5	45.0	2
CGTTTTA	225	0.0	43.0	1
CGTTTTT	1190	0.0	42.542015	1
GCACGGG	80	0.0	42.1875	3
ACGAGCG	65	0.0	41.53846	16
GGCGATT	80	0.0	39.375	8
CGGTTTT	75	0.0	39.000004	1
ACAACGA	70	0.0	38.571426	13
AGCGACT	70	0.0	38.571426	19
GCGATCA	35	6.2261333E-6	38.571426	9
>>END_MODULE