##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936332.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102452
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.926629055557726	33.0	31.0	34.0	30.0	34.0
2	32.03833990551673	33.0	31.0	34.0	30.0	34.0
3	31.94427634404404	33.0	31.0	34.0	30.0	34.0
4	35.702602194198256	37.0	35.0	37.0	35.0	37.0
5	35.77789599031742	37.0	35.0	37.0	35.0	37.0
6	35.764553156600165	37.0	35.0	37.0	35.0	37.0
7	26.86245266075821	35.0	11.0	37.0	0.0	37.0
8	31.152032171163082	35.0	19.0	37.0	17.0	37.0
9	35.81923242103619	39.0	32.0	39.0	32.0	39.0
10	36.62846015695155	38.0	35.0	39.0	33.0	39.0
11	37.01090266661461	39.0	37.0	39.0	34.0	39.0
12	37.027027290828876	39.0	37.0	39.0	34.0	39.0
13	36.92577987740601	39.0	37.0	39.0	33.0	39.0
14	38.090588763518525	40.0	37.0	41.0	33.0	41.0
15	38.16864482879787	40.0	37.0	41.0	33.0	41.0
16	38.15013860149143	40.0	37.0	41.0	33.0	41.0
17	38.091213446296806	40.0	37.0	41.0	33.0	41.0
18	37.97394877601218	40.0	37.0	41.0	33.0	41.0
19	37.889177370866356	40.0	37.0	41.0	33.0	41.0
20	37.93236832858314	40.0	36.0	41.0	33.0	41.0
21	37.90283254597275	40.0	36.0	41.0	33.0	41.0
22	37.89861593721938	40.0	36.0	41.0	33.0	41.0
23	37.86610315074376	40.0	36.0	41.0	33.0	41.0
24	37.810564947487606	40.0	36.0	41.0	33.0	41.0
25	37.71973216725881	40.0	36.0	41.0	33.0	41.0
26	37.57415179791512	39.0	36.0	41.0	33.0	41.0
27	37.435667434505916	39.0	36.0	41.0	33.0	41.0
28	37.35054464529731	39.0	36.0	41.0	32.0	41.0
29	37.357806582594776	39.0	36.0	41.0	32.0	41.0
30	37.31082848553469	39.0	36.0	41.0	32.0	41.0
31	37.14674188888455	39.0	35.0	41.0	32.0	41.0
32	37.05497208448835	39.0	35.0	41.0	31.0	41.0
33	36.934291180260026	39.0	35.0	41.0	31.0	41.0
34	36.80536251122477	39.0	35.0	41.0	31.0	41.0
35	36.74271854136571	39.0	35.0	41.0	31.0	41.0
36	36.509497130363485	39.0	35.0	41.0	30.0	41.0
37	36.41380353726623	39.0	35.0	41.0	30.0	41.0
38	36.27810096435404	39.0	35.0	41.0	30.0	41.0
39	36.2290438449225	39.0	35.0	40.0	29.0	41.0
40	36.0483738726428	39.0	35.0	40.0	28.0	41.0
41	35.892437434115486	39.0	35.0	40.0	28.0	41.0
42	35.81715339866474	38.0	35.0	40.0	28.0	41.0
43	35.74100066372545	38.0	35.0	40.0	28.0	41.0
44	35.5002732987155	38.0	34.0	40.0	26.0	41.0
45	35.37346269472533	38.0	34.0	40.0	26.0	41.0
46	35.181128723695	38.0	34.0	40.0	26.0	41.0
47	35.001141998204034	38.0	34.0	40.0	24.0	41.0
48	34.89279857884668	38.0	34.0	40.0	24.0	41.0
49	34.70754109241401	38.0	34.0	40.0	24.0	41.0
50	34.4637195955179	37.0	33.0	40.0	23.0	41.0
51	32.223899972670125	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	10.0
10	10.0
11	11.0
12	5.0
13	3.0
14	4.0
15	3.0
16	14.0
17	21.0
18	47.0
19	77.0
20	122.0
21	226.0
22	312.0
23	452.0
24	620.0
25	853.0
26	1039.0
27	1332.0
28	1462.0
29	1759.0
30	2236.0
31	2784.0
32	3460.0
33	4790.0
34	7217.0
35	8972.0
36	9515.0
37	14794.0
38	19526.0
39	20767.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.16019208995432	19.507671885370712	23.938039276929686	12.394096747745285
2	31.809042283215554	25.909694295865382	24.686682544020613	17.59458087689845
3	32.65138796704798	25.08882208253621	24.938507789013393	17.32128216140241
4	28.899387030023817	25.82672861437551	26.095146995666262	19.17873735993441
5	26.14883067192441	29.371803381095535	24.349939483855852	20.129426463124194
6	24.92484285323859	36.53613399445594	25.09663061726467	13.442392535040801
7	58.81973997579354	28.455276617342758	7.9149260141334485	4.810057392730254
8	77.59926599773553	8.06816850817944	9.20723851169328	5.125326982391754
9	70.09233592316401	10.24284543005505	11.531253660250654	8.133564986530278
10	39.95041580447429	27.868660445867334	18.54722211377035	13.633701635888027
11	31.795377347440752	26.259126224963886	24.374341154882288	17.571155272713078
12	32.71776051223988	22.593019169952758	27.471401241557018	17.21781907625034
13	25.302580720727757	27.142466716120722	27.88720571584742	19.667746847304105
14	18.848826767657048	31.297583258501543	27.15710771873658	22.69648225510483
15	18.054308359036426	28.799828212235973	34.92464763987038	18.22121578885722
16	20.312927029242964	26.377230312731815	32.70995197751142	20.599890680513802
17	20.747276773513452	25.47534455159489	29.369851247413425	24.407527427478236
18	20.809745051341118	28.461133018389102	29.587514152969195	21.14160777730059
19	24.05809549837973	28.84765548744778	26.292312497559834	20.801936516612656
20	26.083434193573574	26.90235427322063	28.188810369734117	18.825401163471675
21	24.391910358021317	28.494319290985047	27.259594737047593	19.854175613946044
22	21.312419474485615	27.56217545777535	25.993636044196304	25.131769023542734
23	22.25237184242377	28.774450474368486	27.230312731815875	21.742864951391873
24	22.557880763674696	26.461172061062744	28.26494358333659	22.716003591925976
25	20.652598289930896	29.067270526685668	27.06633350251825	23.213797680865188
26	21.142583844141647	29.574825284035448	27.219575996564245	22.06301487525866
27	23.44805372271893	30.629953539218363	25.52805216101199	20.393940577050717
28	20.05817358372701	29.475266466247607	28.915004099480733	21.551555850544645
29	22.16354975988756	27.100495841955258	28.140007027681257	22.59594737047593
30	23.073244055752937	28.45820481786593	26.150782805606525	22.317768320774604
31	22.506149221098664	29.311287236949983	25.270370514972868	22.912193026978485
32	24.300160074961934	29.379611915823993	24.91020185062273	21.410026158591343
33	23.371920509116464	29.366923046890253	24.74231835396088	22.518838090032407
34	21.80338109553742	28.3674306016476	26.909186741108034	22.920001561706947
35	23.394370046460782	25.22156717292	27.13465818139226	24.249404599226956
36	23.54956467418889	29.861788935306286	25.76621247022996	20.82243392027486
37	22.23773083980791	30.225861867020654	27.919415921602308	19.616991371569124
38	20.45445672119627	28.794947878030687	26.912114941631206	23.83848045914184
39	21.584742123140593	26.719829773942923	27.866708312185217	23.82871979073127
40	22.59594737047593	25.026353804708545	31.016476008277046	21.36122281653848
41	19.91957209229688	27.64416507242416	29.8159137937766	22.620349041502365
42	21.014719087963144	28.053137078827156	28.64463358450787	22.28751024870183
43	24.44071370007418	26.915043142154378	26.376254245890756	22.267988911880686
44	22.034708936867997	27.786670831218522	26.295240698083006	23.883379533830475
45	20.916136337016358	27.155155585054462	26.516807871003007	25.41190020692617
46	22.077655877874516	29.153164408698707	26.564635146214812	22.20454456721196
47	21.348533947604732	27.086830906180452	30.178034591808846	21.386600554405966
48	21.630617264670285	24.997071799476828	29.842267598485144	23.530043337367744
49	20.09624019052825	26.55975481200953	29.120954202943818	24.223050794518407
50	19.502791551165423	26.29719283176512	29.41475032210206	24.7852652949674
51	19.708741654628508	25.719361261859213	27.888181782688477	26.683715300823803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	184.0
2	301.0
3	377.5
4	454.0
5	348.0
6	242.0
7	230.5
8	219.0
9	218.5
10	218.0
11	220.5
12	223.0
13	225.0
14	227.0
15	240.0
16	253.0
17	271.0
18	289.0
19	299.5
20	310.0
21	373.0
22	436.0
23	522.0
24	608.0
25	796.0
26	1083.0
27	1182.0
28	1415.5
29	1649.0
30	1936.0
31	2223.0
32	2437.0
33	2651.0
34	2924.0
35	3197.0
36	3404.5
37	3612.0
38	4046.5
39	4481.0
40	4858.5
41	5236.0
42	5872.0
43	6508.0
44	7216.5
45	7925.0
46	8817.5
47	9710.0
48	9345.5
49	8981.0
50	8437.5
51	7894.0
52	6915.0
53	5936.0
54	5417.0
55	4898.0
56	4729.5
57	4561.0
58	4436.5
59	4312.0
60	4164.0
61	4016.0
62	3635.0
63	3254.0
64	2754.5
65	2255.0
66	1695.5
67	1136.0
68	947.5
69	759.0
70	645.5
71	532.0
72	402.0
73	272.0
74	222.0
75	143.5
76	115.0
77	95.0
78	75.0
79	56.5
80	38.0
81	29.5
82	21.0
83	14.5
84	8.0
85	8.0
86	8.0
87	4.0
88	0.0
89	0.0
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	102452.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.557880763674696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.91938264510303	34.203334244329056
2	9.506608652819965	9.042283215554601
3	4.933913471800345	7.0393940577050715
4	3.3433215663738607	6.360051536329207
5	2.434118709465561	5.788076367469644
6	1.7301535177735818	4.9369460820677
7	1.4181922666447748	4.721235310194042
8	1.0631311058205402	4.04482098934135
9	0.8496839339955669	3.636825049779409
>10	2.754289467203021	17.632647483699685
>50	0.03283802643461128	1.0746495920040604
>100	0.014366636565142435	1.519736071526178
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG	353	0.3445515948932183	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	265	0.258657712880178	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	246	0.2401124429000898	No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	192	0.1874048334829969	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	189	0.18447663295982508	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	185	0.18057236559559597	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	127	0.12396048881427399	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15909889509233593	0.0
2	0.0	0.0	0.0	0.8198961464881115	0.0
3	0.0	0.0	0.0	1.059032522547144	0.0
4	0.0	0.0	0.0	1.3782063795728732	0.0
5	0.0	0.0	0.0	2.539725920431031	0.0
6	0.0	0.0	0.0	3.046304610939757	0.0
7	0.0	0.0	0.0	3.62218404716355	0.0
8	0.0	0.0	0.0	4.5113809393667275	0.0
9	0.0	0.0	0.0	4.876429937922149	0.0
10	0.0	0.0	0.0	6.151173232342951	0.0
11	0.0	0.0	0.0	7.292195369538906	0.0
12	0.0	0.0	0.0	8.532776324522704	0.0
13	0.0	0.0	0.0	8.978838870885879	0.0
14	0.0	0.0	0.0	9.17893257330262	0.0
15	0.0	0.0	0.0	9.528364502401125	0.0
16	0.0	0.0	0.0	10.229180494280248	0.0
17	0.0	0.0	0.0	10.960254558232148	0.0
18	0.0	0.0	0.0	11.734275563190566	0.0
19	0.0	0.0	0.0	12.172529574825283	0.0
20	0.0	0.0	0.0	12.623472455393745	0.0
21	0.0	0.0	0.0	13.14859641588256	0.0
22	0.0	0.0	0.0	13.72838011947058	0.0
23	0.0	0.0	0.0	14.254480146800454	0.0
24	0.0	0.0	0.0	14.710303361574201	0.0
25	0.0	0.0	0.0	15.121227501659314	0.0
26	0.0	0.0	0.0	15.533127708585484	0.0
27	0.0	0.0	0.0	15.88548783820716	0.0
28	0.0	0.0	0.0	16.271034240424783	0.0
29	0.0	0.0	0.0	16.669269511576154	0.0
30	0.0	0.0	0.0	17.09678678795924	0.0
31	0.0	0.0	0.0	17.56627493850779	0.0
32	0.0	0.0	0.0	17.993792214890874	0.0
33	9.760668410572756E-4	0.0	0.0	18.427165892320307	0.0
34	9.760668410572756E-4	0.0	0.0	18.85273103502128	0.0
35	9.760668410572756E-4	0.0	0.0	19.302697848748682	0.0
36	9.760668410572756E-4	0.0	0.0	19.77316206613829	0.0
37	9.760668410572756E-4	0.0	0.0	20.204583609885606	0.0
38	9.760668410572756E-4	0.0	0.0	20.645765822043494	0.0
39	9.760668410572756E-4	0.0	0.0	21.06645063053918	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	25	3.8658953E-5	45.0	13
GTGTTAT	20	7.003207E-4	45.0	21
TTGATAT	20	7.003207E-4	45.0	42
CATATCA	20	7.003207E-4	45.0	29
TCACATA	20	7.003207E-4	45.0	33
CCCTAGC	25	3.8658953E-5	45.0	21
CATGAGG	25	3.8658953E-5	45.0	2
TGAAACG	25	3.8658953E-5	45.0	4
CCTCGGG	20	7.003207E-4	45.0	3
TAGTTAA	20	7.003207E-4	45.0	38
AACGGCT	25	3.8658953E-5	45.0	7
ATATCAC	20	7.003207E-4	45.0	30
CAAGGGT	20	7.003207E-4	45.0	5
AACGAGC	25	3.8658953E-5	45.0	15
GACGGAA	20	7.003207E-4	45.0	5
GGACGGA	20	7.003207E-4	45.0	4
TTAACCA	20	7.003207E-4	45.0	14
CAGTCTC	25	3.8658953E-5	45.0	6
ATAGTTA	20	7.003207E-4	45.0	37
ATTATAC	25	3.8658953E-5	45.0	42
>>END_MODULE