##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936330.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200799
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.923410973162216	33.0	31.0	34.0	30.0	34.0
2	32.04132988710103	33.0	31.0	34.0	30.0	34.0
3	31.491860019223203	33.0	31.0	34.0	28.0	34.0
4	35.46856807055812	37.0	35.0	37.0	33.0	37.0
5	35.735725775526774	37.0	35.0	37.0	35.0	37.0
6	35.76646298039333	37.0	35.0	37.0	35.0	37.0
7	27.149542577403274	36.0	18.0	37.0	0.0	37.0
8	31.380425201320723	37.0	25.0	37.0	17.0	37.0
9	36.15298382960074	39.0	32.0	39.0	32.0	39.0
10	36.80311156928072	38.0	37.0	39.0	33.0	39.0
11	37.095692707633006	39.0	37.0	39.0	34.0	39.0
12	37.28160996817713	39.0	37.0	39.0	34.0	39.0
13	37.37870706527423	39.0	37.0	39.0	35.0	39.0
14	38.66078516327273	40.0	38.0	41.0	35.0	41.0
15	38.72000856577971	40.0	38.0	41.0	35.0	41.0
16	38.62281684669745	40.0	38.0	41.0	34.0	41.0
17	38.5365464967455	40.0	38.0	41.0	34.0	41.0
18	38.350569474947584	40.0	38.0	41.0	34.0	41.0
19	38.16516516516516	40.0	37.0	41.0	34.0	41.0
20	38.1167784700123	40.0	37.0	41.0	34.0	41.0
21	38.063058082958584	40.0	36.0	41.0	34.0	41.0
22	38.05133989711104	40.0	36.0	41.0	34.0	41.0
23	37.99795317705765	40.0	36.0	41.0	34.0	41.0
24	37.934860233367694	40.0	36.0	41.0	34.0	41.0
25	37.84903311271471	40.0	36.0	41.0	33.0	41.0
26	37.763743843345836	40.0	36.0	41.0	33.0	41.0
27	37.64794147381212	40.0	36.0	41.0	33.0	41.0
28	37.61130782523817	40.0	36.0	41.0	33.0	41.0
29	37.58211445276122	40.0	36.0	41.0	33.0	41.0
30	37.504758489833115	40.0	36.0	41.0	33.0	41.0
31	37.37075881852001	40.0	35.0	41.0	33.0	41.0
32	37.16610142480789	40.0	35.0	41.0	32.0	41.0
33	36.94747981812658	40.0	35.0	41.0	31.0	41.0
34	36.684585082595035	39.0	35.0	41.0	30.0	41.0
35	36.42342342342342	39.0	35.0	41.0	30.0	41.0
36	36.18795910338199	39.0	35.0	41.0	29.0	41.0
37	36.038097799291826	39.0	35.0	41.0	27.0	41.0
38	35.890457621800905	39.0	35.0	41.0	27.0	41.0
39	35.81731980736956	39.0	35.0	41.0	26.0	41.0
40	35.6795003959183	39.0	35.0	41.0	25.0	41.0
41	35.61748315479659	39.0	35.0	41.0	25.0	41.0
42	35.504609086698636	39.0	35.0	41.0	24.0	41.0
43	35.44122729694869	39.0	35.0	41.0	24.0	41.0
44	35.24090757424091	39.0	35.0	40.0	23.0	41.0
45	35.16405958197003	39.0	35.0	40.0	23.0	41.0
46	35.04553309528434	39.0	35.0	40.0	23.0	41.0
47	34.90010408418369	38.0	34.0	40.0	23.0	41.0
48	34.83615456252272	38.0	34.0	40.0	22.0	41.0
49	34.67469957519709	38.0	34.0	40.0	21.0	41.0
50	34.49411600655382	38.0	34.0	40.0	20.0	41.0
51	32.65695048282113	36.0	31.0	40.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	18.0
10	17.0
11	12.0
12	7.0
13	4.0
14	0.0
15	5.0
16	11.0
17	25.0
18	51.0
19	121.0
20	208.0
21	387.0
22	583.0
23	994.0
24	1530.0
25	2473.0
26	3297.0
27	3586.0
28	3501.0
29	3531.0
30	3881.0
31	4512.0
32	5664.0
33	7656.0
34	11200.0
35	15107.0
36	17542.0
37	28770.0
38	39154.0
39	46942.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.614226166464974	20.533966802623517	25.23916951777648	17.612637513135027
2	34.95286331107227	22.73069088989487	26.484693648872753	15.831752150160112
3	29.504629007116566	22.889556222889556	32.648568966976924	14.957245803016948
4	25.021538951887212	25.857200484066155	32.23073820088745	16.89052236315918
5	23.621631581830588	28.79845019148502	30.353238810950256	17.226679415734143
6	23.763066549136198	33.182436167510794	29.810407422347723	13.244089861005284
7	60.11434319892032	26.254612821777002	10.475649779132366	3.1553942001703197
8	82.26534992704147	3.6145598334653064	11.661412656437532	2.4586775830556924
9	76.53026160488847	6.877026280011354	13.182336565421144	3.4103755496790322
10	34.34877663733385	37.10128038486247	19.02499514439813	9.524947833405545
11	20.50956429065882	25.807399439240235	36.44938470809117	17.233651562009772
12	21.462258278178677	23.522527502627007	36.78006364573529	18.23515057345903
13	21.155981852499263	24.319842230289993	36.4698031364698	18.05437278074094
14	17.694311226649535	27.279020313846186	35.294996489026346	19.73167197047794
15	16.70277242416546	28.790980034761127	36.49071957529669	18.015527965776723
16	20.47669560107371	28.4971538702882	34.04449225344748	16.981658275190615
17	20.625102714654954	27.098242521128093	33.1799461152695	19.096708648947455
18	20.67888784306695	26.935393104547334	34.5544549524649	17.831264099920816
19	19.84472034223278	28.78599993027854	32.99369020762056	18.375589519868125
20	21.049905627020056	28.329324349224848	33.302456685541266	17.318313338213837
21	21.672916697792317	27.46079412746079	34.228756119303384	16.637533055443505
22	20.60169622358677	25.126121146021646	34.37815925378114	19.89402337661044
23	18.55337924989666	27.18589236002171	34.65505306301326	19.60567532706836
24	17.98514932843291	26.832802952205938	36.08982116444803	19.092226554913122
25	18.493119985657298	29.217774988919267	33.33183930198856	18.957265723434876
26	18.564335479758366	28.833808933311424	33.035025074826066	19.566830512104143
27	17.469210504036376	28.619664440559962	34.676467512288404	19.234657543115254
28	16.611636512134027	28.20033964312571	35.31541491740497	19.872608927335296
29	17.299388941179984	26.322342242740255	36.09579728982714	20.28247152625262
30	18.501586163277707	26.89206619554878	34.85525326321346	19.751094377960047
31	18.464733390106524	28.567871353941005	32.936917016519004	20.030478239433464
32	18.6066663678604	28.283507387985	32.92745481800208	20.18237142615252
33	18.596208148446955	27.343263661671624	33.34080349005722	20.7197246998242
34	17.720207769959014	27.108700740541536	33.86919257566024	21.301898913839214
35	18.0304682792245	27.039975298681767	33.53054547084398	21.399010951249757
36	19.084258387740974	27.31288502432781	32.53402656387731	21.068830024053906
37	18.315828266077023	28.687393861523212	32.31141589350545	20.685361978894317
38	18.24809884511377	28.630620670421663	31.672966498837145	21.44831398562742
39	18.977186141365245	28.22275011329738	31.248163586472046	21.551900158865333
40	19.38256664624824	28.008605620545918	31.265095941712858	21.343731791492985
41	18.807862588957118	26.896050279134858	31.87067664679605	22.42541048511198
42	19.596213128551437	27.446849834909536	31.887609002036864	21.069328034502163
43	19.31931931931932	27.809401441242237	31.258123795437225	21.613155444001215
44	18.57927579320614	28.496655859839944	30.240190439195413	22.683877907758504
45	18.582761866343954	28.031514101165843	30.802942245728314	22.582781786761885
46	19.427387586591564	28.066872842992247	30.473259328980724	22.032480241435465
47	18.394015906453717	27.86268855920597	32.01360564544644	21.72968988889387
48	18.67389777837539	26.96278367920159	32.458328975741914	21.90498956668111
49	19.154477860945523	26.67393761921125	32.20284961578494	21.968734904058287
50	18.180867434598778	26.408000039840836	32.98472601955189	22.426406506008494
51	17.989133412018983	26.45531103242546	31.528045458393716	24.027510097161837
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	82.0
1	177.5
2	273.0
3	3086.5
4	5900.0
5	4034.0
6	2168.0
7	1883.5
8	1599.0
9	1518.0
10	1437.0
11	1429.0
12	1421.0
13	1361.0
14	1301.0
15	1289.0
16	1277.0
17	1212.5
18	1148.0
19	1142.5
20	1137.0
21	1217.0
22	1297.0
23	1440.5
24	1584.0
25	1799.0
26	2458.5
27	2903.0
28	3498.5
29	4094.0
30	4802.0
31	5510.0
32	6344.5
33	7179.0
34	8027.0
35	8875.0
36	9227.0
37	9579.0
38	10067.0
39	10555.0
40	11267.5
41	11980.0
42	12510.0
43	13040.0
44	13426.5
45	13813.0
46	14213.5
47	14614.0
48	15371.0
49	16128.0
50	15989.5
51	15851.0
52	14630.0
53	13409.0
54	11281.0
55	9153.0
56	7810.5
57	6468.0
58	5576.5
59	4685.0
60	4131.0
61	3577.0
62	3081.5
63	2586.0
64	2156.5
65	1727.0
66	1399.0
67	1071.0
68	838.0
69	605.0
70	476.0
71	347.0
72	270.0
73	193.0
74	144.5
75	77.5
76	59.0
77	51.0
78	43.0
79	26.0
80	9.0
81	8.0
82	7.0
83	4.0
84	1.0
85	1.0
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	200799.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.420571329823304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.13780728658371	37.29084501113585
2	11.752282403082274	12.321227159980031
3	5.691188030171323	8.950059842610973
4	3.5616719600004516	7.468195161305409
5	2.3472882254782657	6.15230949276689
6	1.6027356846324212	5.040979216747621
7	1.1267010122436294	4.13436175457909
8	0.772423532516163	3.2392706306478085
9	0.5788186326578801	2.7307803078245874
>10	1.4041341538610501	9.320226350135592
>50	0.011975557810964809	0.4498986674182741
>100	0.010977594660051077	0.8474835362995395
>500	9.979631509137341E-4	0.30080434158779956
>1k	9.979631509137341E-4	1.753558526960529
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3352	1.6693310225648534	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	575	0.2863560077490426	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	348	0.17330763599420315	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	244	0.1215145493752459	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.980104482592045E-4	0.0	0.0	0.10956229861702499	0.0
2	4.980104482592045E-4	0.0	0.0	0.35259139736751677	0.0
3	4.980104482592045E-4	0.0	0.0	0.5244050020169423	0.0
4	4.980104482592045E-4	0.0	0.0	0.6997046798041823	0.0
5	4.980104482592045E-4	0.0	0.0	1.2524962773718993	0.0
6	4.980104482592045E-4	0.0	0.0	1.7385544748728827	0.0
7	4.980104482592045E-4	0.0	0.0	2.1608673349966883	0.0
8	4.980104482592045E-4	0.0	0.0	2.8839785058690532	0.0
9	4.980104482592045E-4	0.0	0.0	3.241051997270903	0.0
10	4.980104482592045E-4	0.0	0.0	3.942748718868122	0.0
11	4.980104482592045E-4	0.0	0.0	4.761477895806254	0.0
12	4.980104482592045E-4	0.0	0.0	5.472138805472139	0.0
13	4.980104482592045E-4	0.0	0.0	5.825726223736174	0.0
14	4.980104482592045E-4	0.0	0.0	5.99704181793734	0.0
15	4.980104482592045E-4	0.0	0.0	6.203218143516651	0.0
16	4.980104482592045E-4	0.0	0.0	6.650431526053417	0.0
17	4.980104482592045E-4	0.0	0.0	7.1743385176220995	0.0
18	4.980104482592045E-4	0.0	0.0	7.763982888360998	0.0
19	4.980104482592045E-4	0.0	0.0	8.109104129004626	0.0
20	4.980104482592045E-4	0.0	0.0	8.485600027888585	0.0
21	4.980104482592045E-4	0.0	0.0	8.91986513877061	0.0
22	4.980104482592045E-4	0.0	0.0	9.416879566133298	0.0
23	4.980104482592045E-4	0.0	0.0	9.910905930806429	0.0
24	4.980104482592045E-4	0.0	0.0	10.273457537139128	0.0
25	4.980104482592045E-4	0.0	0.0	10.608120558369315	0.0
26	4.980104482592045E-4	0.0	0.0	10.921369130324354	0.0
27	4.980104482592045E-4	0.0	0.0	11.259020214244094	0.0
28	4.980104482592045E-4	0.0	0.0	11.592189204129502	0.0
29	4.980104482592045E-4	0.0	0.0	11.974163217944312	0.0
30	4.980104482592045E-4	0.0	0.0	12.409922360171116	0.0
31	4.980104482592045E-4	0.0	0.0	12.815800875502369	0.0
32	4.980104482592045E-4	0.0	0.0	13.19677886842066	0.0
33	4.980104482592045E-4	0.0	0.0	13.59767727926932	0.0
34	4.980104482592045E-4	0.0	0.0	13.970189094567203	0.0
35	4.980104482592045E-4	0.0	0.0	14.363119338243717	0.0
36	4.980104482592045E-4	0.0	0.0	14.743599320713749	0.0
37	4.980104482592045E-4	0.0	0.0	15.148481815148482	0.0
38	4.980104482592045E-4	0.0	0.0	15.53045582896329	0.0
39	4.980104482592045E-4	0.0	0.0	15.964720939845318	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAG	25	3.8792725E-5	45.0	14
GGGTCGT	20	7.0193666E-4	45.0	8
GGGTACG	25	3.8792725E-5	45.0	7
ACTTCGG	20	7.0193666E-4	45.0	41
CGTAGGA	20	7.0193666E-4	45.0	4
GAGCGCT	20	7.0193666E-4	45.0	36
CGGGCTC	20	7.0193666E-4	45.0	6
CGAATAT	25	3.8792725E-5	45.0	14
GCGATTA	20	7.0193666E-4	45.0	9
CGGTGGG	20	7.0193666E-4	45.0	3
CGAGCGA	25	3.8792725E-5	45.0	17
AGCCTTC	20	7.0193666E-4	45.0	39
CACAACG	25	3.8792725E-5	45.0	12
GCGCTTG	20	7.0193666E-4	45.0	38
CGCTTGC	20	7.0193666E-4	45.0	39
CCTCTCG	25	3.8792725E-5	45.0	31
CGTTTTT	1200	0.0	43.3125	1
CGTTTTA	215	0.0	41.860466	1
TAACGGG	60	3.6379788E-12	41.249996	3
CATACGA	55	6.002665E-11	40.909092	18
>>END_MODULE