##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936329.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	201218
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.900043733662	33.0	31.0	34.0	30.0	34.0
2	32.02146428251945	33.0	31.0	34.0	30.0	34.0
3	31.518636503692512	33.0	31.0	34.0	28.0	34.0
4	35.476711824985834	37.0	35.0	37.0	33.0	37.0
5	35.70413183711199	37.0	35.0	37.0	35.0	37.0
6	35.729875060879245	37.0	35.0	37.0	35.0	37.0
7	26.871676490174835	35.0	11.0	37.0	0.0	37.0
8	31.194406067051656	36.0	19.0	37.0	17.0	37.0
9	35.9950849327595	39.0	32.0	39.0	32.0	39.0
10	36.73454661113817	38.0	35.0	39.0	33.0	39.0
11	37.05627230168275	39.0	37.0	39.0	34.0	39.0
12	37.20501148008628	39.0	37.0	39.0	34.0	39.0
13	37.22012941188164	39.0	37.0	39.0	34.0	39.0
14	38.49606894015446	40.0	38.0	41.0	34.0	41.0
15	38.559000685823335	40.0	38.0	41.0	34.0	41.0
16	38.461206253913666	40.0	38.0	41.0	34.0	41.0
17	38.34400003975787	40.0	38.0	41.0	34.0	41.0
18	38.15634784164438	40.0	37.0	41.0	33.0	41.0
19	37.994354381814745	40.0	37.0	41.0	34.0	41.0
20	37.941163315409156	40.0	36.0	41.0	34.0	41.0
21	37.8804182528402	40.0	36.0	41.0	33.0	41.0
22	37.89214682583069	40.0	36.0	41.0	33.0	41.0
23	37.832246618095795	40.0	36.0	41.0	33.0	41.0
24	37.75085727916986	40.0	35.0	41.0	33.0	41.0
25	37.656695723046646	39.0	35.0	41.0	33.0	41.0
26	37.537173612698666	39.0	35.0	41.0	33.0	41.0
27	37.43383792702442	39.0	35.0	41.0	33.0	41.0
28	37.385666292280014	39.0	35.0	41.0	33.0	41.0
29	37.35268713534575	39.0	35.0	41.0	33.0	41.0
30	37.25757139023348	39.0	35.0	41.0	33.0	41.0
31	37.09163693108967	39.0	35.0	41.0	32.0	41.0
32	36.88586508165273	39.0	35.0	41.0	31.0	41.0
33	36.644659026528444	39.0	35.0	41.0	31.0	41.0
34	36.36431134391555	39.0	35.0	41.0	30.0	41.0
35	36.12731465375861	39.0	35.0	41.0	29.0	41.0
36	35.87166158097188	39.0	35.0	41.0	27.0	41.0
37	35.75405778807065	39.0	35.0	41.0	26.0	41.0
38	35.60183482591021	39.0	35.0	41.0	25.0	41.0
39	35.56873142561798	39.0	35.0	41.0	25.0	41.0
40	35.38843443429514	39.0	35.0	40.0	25.0	41.0
41	35.26846007812422	39.0	35.0	40.0	24.0	41.0
42	35.19854088600423	38.0	35.0	40.0	24.0	41.0
43	35.16397638382252	38.0	35.0	40.0	24.0	41.0
44	34.9749624785059	38.0	34.0	40.0	23.0	41.0
45	34.896122613285094	38.0	34.0	40.0	23.0	41.0
46	34.78162490433262	38.0	34.0	40.0	23.0	41.0
47	34.672474629506304	38.0	34.0	40.0	23.0	41.0
48	34.59098092615969	38.0	34.0	40.0	22.0	41.0
49	34.418014292955895	38.0	34.0	40.0	22.0	41.0
50	34.251289646055525	38.0	33.0	40.0	20.0	41.0
51	32.27896609647248	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	13.0
10	12.0
11	16.0
12	7.0
13	12.0
14	7.0
15	5.0
16	20.0
17	42.0
18	75.0
19	132.0
20	245.0
21	418.0
22	665.0
23	1056.0
24	1637.0
25	2537.0
26	3447.0
27	3689.0
28	3769.0
29	3791.0
30	4104.0
31	5050.0
32	6419.0
33	8351.0
34	12372.0
35	16112.0
36	17933.0
37	28822.0
38	37345.0
39	43102.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.7227683408045	19.727360375314333	24.653361031319264	17.8965102525619
2	35.53210945342862	24.121599459292906	24.1116599906569	16.234631096621573
3	30.759176614418195	23.095349322625214	30.453040980429186	15.692433082527407
4	26.328161496486395	23.63903825701478	31.989185858124024	18.0436143883748
5	24.461032313212534	28.54217813515689	28.87167152044052	18.125118031190052
6	24.538560168573387	32.81813754236699	29.416354401693685	13.226947887365942
7	57.40589808068861	27.452315399218758	11.295709131389836	3.8460773887027995
8	78.15851464580703	5.233130236857537	13.102207555984059	3.50614756135137
9	72.12823902434175	7.466031865936447	14.830681151785626	5.575047957936169
10	36.69651820413681	29.739884105795706	21.275929588804182	12.287668101263307
11	25.541452553946463	25.940522219682137	31.83512409426592	16.682901132105478
12	26.410659086165257	22.622230615551292	33.29821387748611	17.668896420797346
13	22.73603753143357	23.155483107873053	35.00581458915206	19.102664771541313
14	18.352731862954606	26.723752348199465	33.37524475941516	21.548271029430765
15	17.86470395292668	27.025415221302268	36.824737349541294	18.285143476229763
16	21.219274617578947	26.408174219006252	33.80761164508145	18.56493951833335
17	20.35503781967816	26.015068234452187	33.321571628780724	20.30832231708893
18	20.316770865429532	26.980687612440242	32.81366478148078	19.888876740649444
19	22.048723275253703	28.444771342524028	30.492798854973213	19.013706527249052
20	23.287678040731944	26.45638064189089	32.775894800663956	17.480046516713216
21	23.006391078332953	26.838553210945342	32.217296663320376	17.937759047401325
22	21.605422974087805	24.607142502161835	32.45932272460714	21.32811179914322
23	21.014024590245405	26.421095528233064	33.08401832838017	19.480861553141366
24	20.745161963641422	24.664791420250673	34.10281386357085	20.48723275253705
25	20.590106252919718	27.37975727817591	31.647268137045394	20.382868331858976
26	20.095617688278384	27.871264002226443	31.233289268355712	20.79982904113946
27	20.400262401971993	28.122732558717413	31.935015754057787	19.54198928525281
28	18.52269677663032	28.046198650220163	33.21472234094365	20.216382232205866
29	19.70251170372432	26.12241449572106	32.554741623512804	21.620332177041814
30	20.211909471319665	26.777425478833898	32.56269319842161	20.447971851424825
31	20.115496625550396	26.852965440467553	31.828166466220715	21.203371467761333
32	21.01849735113161	27.833494021409617	30.204554264529023	20.94345436292976
33	20.050393105984554	28.020853005198344	31.072269876452406	20.8564840123647
34	19.519128507389997	27.273404963770638	31.990179804987623	21.217286723851743
35	19.44706735977895	26.38531344114344	31.655219711954196	22.512399487123417
36	19.195101829856178	30.361597868977924	29.808963412815952	20.63433688834995
37	19.458497748710354	29.216074108678153	31.324732379806974	20.000695762804522
38	19.41973382102993	29.161904004611916	29.872079038654594	21.546283135703565
39	20.34410440417855	27.526364440557003	30.66723652953513	21.46229462572931
40	20.386844119313384	27.096482422049718	32.03888320130406	20.477790257332842
41	18.819886888846924	28.274309455416514	31.459412179824863	21.446391475911696
42	19.76314246240396	27.97612539633631	30.84763788527865	21.413094255981076
43	20.76653182120884	27.840948622886618	29.75876909620412	21.633750459700423
44	20.557802979852696	28.396067946207598	29.164885845202715	21.88124322873699
45	20.1159935989822	26.649703306861216	29.682732161138663	23.55157093301792
46	19.74674233915455	28.839865220805294	29.30602629983401	22.107366140206146
47	19.773578904471766	27.29328390104265	31.721317178383647	21.21182001610194
48	19.568328877138228	26.669582244133228	31.2069496764703	22.55513920225825
49	18.867596338299755	27.166058702501765	31.473824409347078	22.492520549851406
50	18.383047242294424	26.40966513930165	32.03888320130406	23.16840441709986
51	18.477472194336492	26.28492480791977	30.588714727310677	24.648888270433062
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	92.0
1	258.0
2	424.0
3	3082.5
4	5741.0
5	3900.0
6	2059.0
7	1785.0
8	1511.0
9	1439.5
10	1368.0
11	1352.5
12	1337.0
13	1257.5
14	1178.0
15	1138.0
16	1098.0
17	1113.5
18	1129.0
19	1144.0
20	1159.0
21	1218.0
22	1277.0
23	1458.0
24	1639.0
25	1835.0
26	2277.0
27	2523.0
28	3103.5
29	3684.0
30	4252.5
31	4821.0
32	5331.5
33	5842.0
34	6587.0
35	7332.0
36	7759.0
37	8186.0
38	8835.0
39	9484.0
40	9988.0
41	10492.0
42	11345.5
43	12199.0
44	14501.5
45	16804.0
46	16047.5
47	15291.0
48	14704.5
49	14118.0
50	12987.0
51	11856.0
52	10845.5
53	9835.0
54	9146.0
55	8457.0
56	8210.5
57	7964.0
58	7710.0
59	7456.0
60	7155.0
61	6854.0
62	6174.0
63	5494.0
64	4638.5
65	3783.0
66	3086.5
67	2390.0
68	1938.0
69	1486.0
70	1269.0
71	1052.0
72	886.5
73	721.0
74	581.0
75	354.5
76	268.0
77	216.0
78	164.0
79	120.5
80	77.0
81	64.5
82	52.0
83	37.0
84	22.0
85	18.0
86	14.0
87	11.5
88	9.0
89	5.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	201218.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.83495504875607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.64283337116997	35.911252589401904
2	11.313904643388623	11.502836679451466
3	5.838759665176695	8.90439255359242
4	3.7653425341975124	7.656440738763994
5	2.5198549862730397	6.404835747828691
6	1.7192172699904922	5.243779958340709
7	1.266423349276288	4.506500182320864
8	0.8456149564301568	3.438943863894628
9	0.6636977907198712	3.0365142621483034
>10	1.3873682733528332	9.267059423072554
>50	0.022989531907137026	0.8129890929330464
>100	0.009995448655276967	0.9059747204372635
>500	0.0029986345965830902	0.8683739748891102
>1k	9.995448655276966E-4	1.5401062129250398
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3031	1.5063264717868183	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC	615	0.30563866055720657	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	548	0.2723414406265841	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG	546	0.27134749376298345	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGC	366	0.18189227603892297	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	287	0.14263137492669642	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.96973431800336E-4	0.0	0.0	0.07106720074744804	0.0
2	4.96973431800336E-4	0.0	0.0	0.32005089007941634	0.0
3	4.96973431800336E-4	0.0	0.0	0.46665805246051545	0.0
4	4.96973431800336E-4	0.0	0.0	0.7315448916100945	0.0
5	4.96973431800336E-4	0.0	0.0	1.5386297448538402	0.0
6	4.96973431800336E-4	0.0	0.0	2.043057778131181	0.0
7	4.96973431800336E-4	0.0	0.0	2.5007703088192903	0.0
8	4.96973431800336E-4	0.0	0.0	3.253188084564999	0.0
9	4.96973431800336E-4	0.0	0.0	3.6303909193014543	0.0
10	4.96973431800336E-4	0.0	0.0	4.612907393970718	0.0
11	4.96973431800336E-4	0.0	0.0	5.319106640558996	0.0
12	4.96973431800336E-4	0.0	0.0	6.072518363168305	0.0
13	4.96973431800336E-4	0.0	0.0	6.341877963204087	0.0
14	4.96973431800336E-4	0.0	0.0	6.471588028903975	0.0
15	4.96973431800336E-4	0.0	0.0	6.669383454760508	0.0
16	4.96973431800336E-4	0.0	0.0	7.014283016429942	0.0
17	4.96973431800336E-4	0.0	0.0	7.4178254430518145	0.0
18	4.96973431800336E-4	0.0	0.0	7.877028894035325	0.0
19	4.96973431800336E-4	0.0	0.0	8.172728085956525	0.0
20	4.96973431800336E-4	0.0	0.0	8.450536234332912	0.0
21	4.96973431800336E-4	0.0	0.0	8.790466061684342	0.0
22	4.96973431800336E-4	0.0	0.0	9.18506296653381	0.0
23	4.96973431800336E-4	0.0	0.0	9.560277907543062	0.0
24	4.96973431800336E-4	0.0	0.0	9.859952886918665	0.0
25	4.96973431800336E-4	0.0	0.0	10.138754982158654	0.0
26	4.96973431800336E-4	0.0	0.0	10.404138794740033	0.0
27	4.96973431800336E-4	0.0	0.0	10.650637616913	0.0
28	4.96973431800336E-4	0.0	0.0	10.935403393334592	0.0
29	4.96973431800336E-4	0.0	0.0	11.248496655368804	0.0
30	4.96973431800336E-4	0.0	0.0	11.603335685674244	0.0
31	4.96973431800336E-4	0.0	0.0	11.913944080549454	0.0
32	4.96973431800336E-4	0.0	0.0	12.227534316015467	0.0
33	4.96973431800336E-4	0.0	0.0	12.518760747050463	0.0
34	4.96973431800336E-4	0.0	0.0	12.82439940760767	0.0
35	4.96973431800336E-4	0.0	0.0	13.13649872277828	0.0
36	4.96973431800336E-4	0.0	0.0	13.456549612857696	0.0
37	4.96973431800336E-4	0.0	0.0	13.783061157550517	0.0
38	4.96973431800336E-4	0.0	0.0	14.120506117742945	0.0
39	4.96973431800336E-4	0.0	0.0	14.466896599707779	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGCT	20	7.019403E-4	45.000004	5
CGAGTAT	20	7.019403E-4	45.000004	27
AATGCGG	20	7.019403E-4	45.000004	2
TACGAAT	30	2.1565502E-6	45.000004	12
TCTAAGG	30	2.1565502E-6	45.000004	3
AACGAAG	20	7.019403E-4	45.000004	1
GGCGATC	40	6.7666406E-9	45.000004	8
GCGCTCA	20	7.019403E-4	45.000004	33
TGTACGG	20	7.019403E-4	45.000004	2
CGGGATA	35	1.2052624E-7	45.0	6
GCTACGA	25	3.8793027E-5	45.0	10
CGTTTTT	1115	0.0	43.789234	1
CGTTTTA	210	0.0	42.857143	1
ATCTATC	50	1.0713848E-9	40.5	3
CGGTTTT	140	0.0	40.17857	1
TGCGAGG	45	1.9146682E-8	40.0	2
ACAGGAT	40	3.4392906E-7	39.375004	5
GGGTTAT	35	6.222632E-6	38.571426	7
AAAGCGG	35	6.222632E-6	38.571426	2
TCTATCT	65	9.094947E-12	38.07692	4
>>END_MODULE