SRR2936327.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936327.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	139013
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	1042	0.749570184083503	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	1038	0.7466927553538159	Illumina PCR Primer Index 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC	707	0.508585527972204	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	571	0.41075295116284083	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	525	0.37766252077143864	Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	325	0.2337910842870811	Illumina PCR Primer Index 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	290	0.2086135829023185	Illumina PCR Primer Index 2 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT	273	0.1963845108011481	No Hit
GAATCTATCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	246	0.17696186687575982	Illumina PCR Primer Index 2 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	241	0.17336508096365089	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTC	199	0.1431520793019358	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	186	0.13380043593045254	Illumina PCR Primer Index 2 (95% over 24bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC	177	0.12732622128865645	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	167	0.12013264946443858	Illumina PCR Primer Index 2 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACCT	20	7.011882E-4	45.0	11
ATAGGGC	20	7.011882E-4	45.0	4
CGCGCGG	20	7.011882E-4	45.0	2
TCGGGAA	25	3.8730737E-5	45.0	5
CAACAGG	20	7.011882E-4	45.0	2
CTGACTA	20	7.011882E-4	45.0	22
TGAAACG	25	3.8730737E-5	45.0	4
CCTCCGC	25	3.8730737E-5	45.0	45
GTGCTAG	20	7.011882E-4	45.0	1
CTACGAA	20	7.011882E-4	45.0	11
TACGAAT	20	7.011882E-4	45.0	12
TCATGCG	20	7.011882E-4	45.0	1
CGGGATC	35	1.2016426E-7	45.0	6
GCGATAG	25	3.8730737E-5	45.0	9
CGTAAGG	20	7.011882E-4	45.0	2
GGACCTA	20	7.011882E-4	45.0	8
GGACCCA	20	7.011882E-4	45.0	8
GCTACGA	20	7.011882E-4	45.0	10
GCTAAAG	20	7.011882E-4	45.0	1
TGAGCGG	20	7.011882E-4	45.0	2