##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936327.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	139013
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67544042643494	31.0	31.0	34.0	30.0	34.0
2	31.783480681662866	31.0	31.0	34.0	30.0	34.0
3	31.65044276434578	31.0	31.0	34.0	30.0	34.0
4	35.46163308467553	37.0	35.0	37.0	33.0	37.0
5	35.58816801306352	37.0	35.0	37.0	33.0	37.0
6	35.551998733931356	37.0	35.0	37.0	33.0	37.0
7	27.039521483602254	35.0	19.0	37.0	0.0	37.0
8	31.18136433283218	35.0	25.0	37.0	17.0	37.0
9	35.82410278175423	39.0	32.0	39.0	32.0	39.0
10	36.4343838346054	37.0	35.0	39.0	32.0	39.0
11	36.73845611561509	38.0	35.0	39.0	33.0	39.0
12	36.9233956536439	39.0	37.0	39.0	33.0	39.0
13	36.90585053196464	39.0	37.0	39.0	33.0	39.0
14	38.057196089574354	40.0	37.0	41.0	33.0	41.0
15	38.07840993288397	40.0	37.0	41.0	33.0	41.0
16	38.00672598965564	40.0	37.0	41.0	33.0	41.0
17	37.89762108579773	40.0	37.0	41.0	33.0	41.0
18	37.77932999072029	39.0	36.0	41.0	33.0	41.0
19	37.68925208433743	39.0	36.0	41.0	33.0	41.0
20	37.772992453943154	39.0	36.0	41.0	33.0	41.0
21	37.743621100184875	39.0	36.0	41.0	33.0	41.0
22	37.77072647881853	39.0	36.0	41.0	33.0	41.0
23	37.742772258709614	39.0	36.0	41.0	33.0	41.0
24	37.69521555537971	39.0	36.0	41.0	33.0	41.0
25	37.62208570421471	39.0	36.0	41.0	33.0	41.0
26	37.53188550711084	39.0	36.0	41.0	33.0	41.0
27	37.4288303971571	39.0	36.0	41.0	32.0	41.0
28	37.42178069676937	39.0	36.0	41.0	32.0	41.0
29	37.4378007812219	39.0	36.0	41.0	33.0	41.0
30	37.33976678440146	39.0	36.0	41.0	32.0	41.0
31	37.09491198664873	39.0	36.0	41.0	31.0	41.0
32	36.98515966132664	39.0	35.0	41.0	31.0	41.0
33	36.95005503082446	39.0	35.0	40.0	31.0	41.0
34	36.79651543380835	39.0	35.0	40.0	30.0	41.0
35	36.76797134080985	39.0	35.0	40.0	30.0	41.0
36	36.55788307568357	39.0	35.0	40.0	30.0	41.0
37	36.46585571133635	39.0	35.0	40.0	30.0	41.0
38	36.291692143900214	39.0	35.0	40.0	30.0	41.0
39	36.21538273398891	39.0	35.0	40.0	30.0	41.0
40	36.053239625071036	38.0	35.0	40.0	29.0	41.0
41	36.06335378705589	38.0	35.0	40.0	29.0	41.0
42	36.0572392510053	39.0	35.0	40.0	29.0	41.0
43	36.05102400494918	38.0	35.0	40.0	29.0	41.0
44	35.81057167315287	38.0	35.0	40.0	28.0	41.0
45	35.70619294598347	38.0	35.0	40.0	28.0	41.0
46	35.47223640954443	38.0	34.0	40.0	27.0	41.0
47	35.28175062763914	38.0	34.0	40.0	26.0	41.0
48	35.19308266133383	38.0	34.0	40.0	26.0	41.0
49	34.93615705006007	38.0	34.0	40.0	24.0	41.0
50	34.641788897441245	38.0	33.0	40.0	23.0	41.0
51	32.09745131750268	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	6.0
11	2.0
12	1.0
13	6.0
14	1.0
15	3.0
16	8.0
17	11.0
18	37.0
19	70.0
20	119.0
21	200.0
22	331.0
23	481.0
24	698.0
25	1078.0
26	1328.0
27	1744.0
28	2083.0
29	2472.0
30	3211.0
31	4049.0
32	5278.0
33	6749.0
34	9784.0
35	12437.0
36	14183.0
37	20592.0
38	26167.0
39	25868.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.90705905203111	21.495111967945444	26.131369008653866	12.466459971369584
2	28.972110522037507	27.73481616827203	25.815571205570702	17.477502104119758
3	29.259134037823802	26.69822246840224	28.292317984648918	15.750325509125046
4	26.333508376914388	24.76099357614036	30.852510196888062	18.05298785005719
5	25.61343183731018	29.012394524253125	26.58312531921475	18.791048319221943
6	22.80937754023005	35.39884758979376	27.370102076784185	14.421672793192004
7	59.98431801342321	26.34142130592103	9.199859005992245	4.474401674663521
8	79.25373885895564	5.6232150949911155	9.514937451892989	5.608108594160258
9	74.19953529526015	6.565573003963658	11.101839396315452	8.133052304460733
10	35.91750411831987	33.42205405249869	18.128520354211478	12.531921474969968
11	22.694999748224987	25.853697136239056	31.026594635034133	20.424708480501824
12	22.730967607346074	22.389272945695726	34.4888607540302	20.390898692928
13	20.18444318157295	26.43421838245344	35.65925488983045	17.722083546143168
14	18.101184781279446	29.787861566903818	30.336011739909218	21.77494191190752
15	15.339572557962205	28.298792199290713	37.55332235114702	18.808312891600064
16	18.57596052167783	29.175688604662874	30.926603986677502	21.321746886981792
17	18.737815887722732	27.5456252292951	30.46693474710998	23.249624135872185
18	19.016207117319965	27.771503384575542	32.770316445224545	20.44197305287995
19	18.5550991633876	29.760525993971786	28.87211987368088	22.81225496895974
20	20.621812348485395	28.902332875342594	31.442383086473928	19.033471689698086
21	20.16573989482998	30.100781941257292	31.163272499694273	18.570205664218452
22	18.85147432254537	25.537899333155895	29.812319711106156	25.79830663319258
23	16.859574284419445	29.44472819088862	31.13018206930287	22.565515455389065
24	19.78879673124096	25.730687058044932	31.445260515203614	23.035255695510493
25	16.88259371425694	30.854668268435326	29.42386683259839	22.838871184709344
26	17.822793551682217	28.49373799572702	29.260572752188647	24.42289570040212
27	19.20827548502658	28.08298504456418	28.874277945228144	23.834461525181098
28	15.81578701272543	27.2880953579881	33.07028839029444	23.825829238992036
29	19.27085955989728	23.76324516412134	30.55757375209513	26.408321523886258
30	18.76227403192507	26.8456906907987	30.828771409868143	23.563263867408086
31	19.266543416802744	27.901707034593887	28.79946479825628	24.03228475034709
32	21.567047686187625	28.474315351801632	26.675922395747158	23.28271456626359
33	18.95506175681411	25.58609626437815	29.37638925855855	26.082452720249183
34	17.730715832332226	26.88093919273737	30.632386899066994	24.755958075863408
35	17.314208023710012	27.232704854941623	28.314618057303992	27.13846906404437
36	18.658686597656335	26.169494939322224	29.979210577428013	25.192607885593432
37	17.994719918281024	28.070755972463008	31.006452633926322	22.928071475329645
38	17.72711904642012	28.59157057253638	30.5798738247502	23.101436556293294
39	20.83258400293498	25.95440714177811	30.98487191845367	22.228136936833245
40	19.61327357873005	26.9082747656694	31.67401609921374	21.80443555638681
41	16.940142288850684	28.494457352909443	27.606770589800956	26.958629768438925
42	19.219065842762905	25.068159093034463	30.386366742678746	25.326408321523886
43	20.868551862056066	23.58124779696863	29.276398610201927	26.273801730773382
44	19.527670074021856	24.848755152395817	29.943962075489345	25.679612698092985
45	19.42480199693554	24.263198405904486	29.663412774344845	26.648586822815133
46	21.86414220252782	26.71260961205067	28.66206757641372	22.76118060900779
47	16.50061505039097	25.70694827102501	33.23861797098113	24.553818707602886
48	18.642860739643055	24.73293864602591	30.28349866559243	26.34070194873861
49	19.242085272600402	22.6295382446246	33.36378612072252	24.76459036205247
50	18.875932466747713	22.84174861343903	32.581125506247616	25.701193413565637
51	18.28318214843216	23.101436556293294	28.9850589513211	29.63032234395344
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	112.0
2	158.0
3	586.0
4	1014.0
5	704.5
6	395.0
7	350.5
8	306.0
9	313.5
10	321.0
11	347.5
12	374.0
13	380.5
14	387.0
15	388.0
16	389.0
17	390.5
18	392.0
19	407.0
20	422.0
21	496.5
22	571.0
23	651.0
24	731.0
25	929.5
26	1367.5
27	1607.0
28	1784.5
29	1962.0
30	2443.5
31	2925.0
32	3454.5
33	3984.0
34	4329.5
35	4675.0
36	4979.5
37	5284.0
38	5802.5
39	6321.0
40	7007.5
41	7694.0
42	8466.5
43	9239.0
44	10807.5
45	12376.0
46	15789.0
47	19202.0
48	17740.5
49	16279.0
50	15018.0
51	13757.0
52	11324.5
53	8892.0
54	7371.0
55	5850.0
56	4892.5
57	3935.0
58	3382.5
59	2830.0
60	2431.0
61	2032.0
62	1694.5
63	1357.0
64	1125.5
65	894.0
66	705.0
67	516.0
68	417.5
69	319.0
70	251.0
71	183.0
72	153.0
73	123.0
74	96.5
75	49.5
76	29.0
77	19.5
78	10.0
79	7.5
80	5.0
81	3.5
82	2.0
83	2.0
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	139013.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.986037277089196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92317402610517	43.83546862523649
2	10.207276124113207	11.633444354125153
3	4.353808477871191	7.44318876651824
4	2.630715241485521	5.996561472668024
5	1.7420283268954024	4.9635645587103365
6	1.0919235527279154	3.733463776769079
7	0.8659648059784392	3.4543531899894253
8	0.6400060592289631	2.917712731902772
9	0.44308111792774374	2.272449339270428
>10	1.0578403898103965	8.256781739837281
>50	0.021459769244363654	0.8855286915612209
>100	0.016410411775101618	1.814218814067749
>500	0.0037870181019465272	1.2970009999064835
>1k	0.002524678734631018	1.4962629394373188
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	1042	0.749570184083503	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	1038	0.7466927553538159	Illumina PCR Primer Index 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC	707	0.508585527972204	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	571	0.41075295116284083	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	525	0.37766252077143864	Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	325	0.2337910842870811	Illumina PCR Primer Index 2 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	290	0.2086135829023185	Illumina PCR Primer Index 2 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT	273	0.1963845108011481	No Hit
GAATCTATCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	246	0.17696186687575982	Illumina PCR Primer Index 2 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	241	0.17336508096365089	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTC	199	0.1431520793019358	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	186	0.13380043593045254	Illumina PCR Primer Index 2 (95% over 24bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC	177	0.12732622128865645	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	167	0.12013264946443858	Illumina PCR Primer Index 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.32371073208980455	0.0
2	0.0	0.0	0.0	1.637976304374411	0.0
3	0.0	0.0	0.0	2.095487472394668	0.0
4	0.0	0.0	0.0	2.773121938235992	0.0
5	0.0	0.0	0.0	5.0527648493306385	0.0
6	0.0	0.0	0.0	5.992964686755915	0.0
7	0.0	0.0	0.0	7.125232891887809	0.0
8	0.0	0.0	0.0	9.151662074769986	0.0
9	0.0	0.0	0.0	9.834332040888262	0.0
10	0.0	0.0	0.0	12.282304532669606	0.0
11	0.0	0.0	0.0	14.284275571349442	0.0
12	0.0	0.0	0.0	16.5049311934855	0.0
13	0.0	0.0	0.0	17.193356017063152	0.0
14	0.0	0.0	0.0	17.4623956032889	0.0
15	0.0	0.0	0.0	17.94076812959939	0.0
16	0.0	0.0	0.0	18.832051678619987	0.0
17	0.0	0.0	0.0	20.02906203016984	0.0
18	0.0	0.0	0.0	21.22031752426032	0.0
19	0.0	0.0	0.0	22.02383949702546	0.0
20	0.0	0.0	0.0	22.746793465359357	0.0
21	0.0	0.0	0.0	23.671167444771353	0.0
22	0.0	0.0	0.0	24.706322430276305	0.0
23	0.0	0.0	0.0	25.73356448677462	0.0
24	0.0	0.0	0.0	26.45076359764914	0.0
25	0.0	0.0	0.0	27.070849488896723	0.0
26	0.0	0.0	0.0	27.6743901649486	0.0
27	0.0	0.0	0.0	28.26138562580478	0.0
28	0.0	0.0	0.0	28.87355858804572	0.0
29	7.193571824217879E-4	0.0	0.0	29.510909051671426	0.0
30	7.193571824217879E-4	0.0	0.0	30.29644709487602	0.0
31	7.193571824217879E-4	0.0	0.0	30.954658916791956	0.0
32	7.193571824217879E-4	0.0	0.0	31.54740923510751	0.0
33	7.193571824217879E-4	0.0	0.0	32.15310798270665	0.0
34	7.193571824217879E-4	0.0	0.0	32.70485494162416	0.0
35	7.193571824217879E-4	0.0	0.0	33.32206340414206	0.0
36	7.193571824217879E-4	0.0	0.0	33.88963622107285	0.0
37	7.193571824217879E-4	0.0	0.0	34.48814139684778	0.0
38	7.193571824217879E-4	0.0	0.0	35.1031917878184	0.0
39	7.193571824217879E-4	0.0	0.0	35.66500974728982	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACCT	20	7.011882E-4	45.0	11
ATAGGGC	20	7.011882E-4	45.0	4
CGCGCGG	20	7.011882E-4	45.0	2
TCGGGAA	25	3.8730737E-5	45.0	5
CAACAGG	20	7.011882E-4	45.0	2
CTGACTA	20	7.011882E-4	45.0	22
TGAAACG	25	3.8730737E-5	45.0	4
CCTCCGC	25	3.8730737E-5	45.0	45
GTGCTAG	20	7.011882E-4	45.0	1
CTACGAA	20	7.011882E-4	45.0	11
TACGAAT	20	7.011882E-4	45.0	12
TCATGCG	20	7.011882E-4	45.0	1
CGGGATC	35	1.2016426E-7	45.0	6
GCGATAG	25	3.8730737E-5	45.0	9
CGTAAGG	20	7.011882E-4	45.0	2
GGACCTA	20	7.011882E-4	45.0	8
GGACCCA	20	7.011882E-4	45.0	8
GCTACGA	20	7.011882E-4	45.0	10
GCTAAAG	20	7.011882E-4	45.0	1
TGAGCGG	20	7.011882E-4	45.0	2
>>END_MODULE