##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936326.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	107119
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88935669675781	31.0	31.0	34.0	30.0	34.0
2	31.99840364454485	33.0	31.0	34.0	30.0	34.0
3	31.923010857084176	33.0	31.0	34.0	30.0	34.0
4	35.68766512010008	37.0	35.0	37.0	35.0	37.0
5	35.752359525387654	37.0	35.0	37.0	35.0	37.0
6	35.712077222528215	37.0	35.0	37.0	35.0	37.0
7	26.847618069623504	35.0	11.0	37.0	0.0	37.0
8	31.08656727564671	35.0	19.0	37.0	17.0	37.0
9	35.762236391303134	38.0	32.0	39.0	32.0	39.0
10	36.54927697233917	37.0	35.0	39.0	32.0	39.0
11	36.92487793948786	39.0	37.0	39.0	33.0	39.0
12	36.995201598222536	39.0	37.0	39.0	33.0	39.0
13	36.96145408377599	39.0	37.0	39.0	33.0	39.0
14	38.1528393655654	40.0	37.0	41.0	33.0	41.0
15	38.22472203810715	40.0	37.0	41.0	33.0	41.0
16	38.153679552647056	40.0	37.0	41.0	33.0	41.0
17	38.017485226710484	40.0	37.0	41.0	33.0	41.0
18	37.91977146911379	40.0	37.0	41.0	33.0	41.0
19	37.82769630037622	40.0	37.0	41.0	33.0	41.0
20	37.916644106087624	40.0	37.0	41.0	33.0	41.0
21	37.86827733642024	40.0	37.0	41.0	33.0	41.0
22	37.880170651331696	40.0	37.0	41.0	33.0	41.0
23	37.8583164517966	40.0	36.0	41.0	33.0	41.0
24	37.767044128492614	40.0	36.0	41.0	33.0	41.0
25	37.67734948981973	39.0	36.0	41.0	33.0	41.0
26	37.52591977146911	39.0	36.0	41.0	33.0	41.0
27	37.42725380184655	39.0	36.0	41.0	32.0	41.0
28	37.371101298555814	39.0	36.0	41.0	32.0	41.0
29	37.37287502683931	39.0	36.0	41.0	32.0	41.0
30	37.32801837209085	39.0	36.0	41.0	32.0	41.0
31	37.15188715353952	39.0	36.0	41.0	31.0	41.0
32	36.97881795013023	39.0	35.0	41.0	31.0	41.0
33	36.91494506110027	39.0	35.0	41.0	31.0	41.0
34	36.78100990487215	39.0	35.0	41.0	30.0	41.0
35	36.61860174198788	39.0	35.0	41.0	30.0	41.0
36	36.31196146341919	39.0	35.0	41.0	30.0	41.0
37	36.24305678731131	39.0	35.0	40.0	30.0	41.0
38	35.99076727751379	39.0	35.0	40.0	29.0	41.0
39	35.941466966644576	39.0	35.0	40.0	28.0	41.0
40	35.66072312101495	38.0	35.0	40.0	26.0	41.0
41	35.4956917073535	38.0	35.0	40.0	26.0	41.0
42	35.46143074524594	38.0	34.0	40.0	26.0	41.0
43	35.33983700370616	38.0	34.0	40.0	25.0	41.0
44	35.158132544179836	38.0	34.0	40.0	24.0	41.0
45	35.064881113527946	38.0	34.0	40.0	24.0	41.0
46	34.81077119838684	38.0	34.0	40.0	23.0	41.0
47	34.66656708893847	38.0	33.0	40.0	23.0	41.0
48	34.48942764588915	38.0	33.0	40.0	23.0	41.0
49	34.23975205145679	37.0	33.0	40.0	22.0	41.0
50	34.098759323742755	37.0	33.0	40.0	22.0	41.0
51	31.671458844836117	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	12.0
10	7.0
11	7.0
12	9.0
13	4.0
14	2.0
15	10.0
16	13.0
17	23.0
18	35.0
19	80.0
20	135.0
21	203.0
22	293.0
23	488.0
24	713.0
25	936.0
26	1232.0
27	1628.0
28	1806.0
29	2122.0
30	2517.0
31	3002.0
32	3841.0
33	5087.0
34	7474.0
35	9078.0
36	10109.0
37	15573.0
38	19890.0
39	20785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54689644227448	19.09931944846386	24.06949280706504	13.284291302196621
2	29.07047302532697	27.0372202877174	25.292431781476676	18.599874905478952
3	30.335421353821452	26.962536991570126	25.98138518843529	16.720656466173132
4	28.534620375470272	24.63241815177513	28.401123983607018	18.431837489147583
5	26.717015655485955	29.285187501750386	23.608323453355613	20.389473389408042
6	25.20654599090731	35.54644834249759	24.389697439296484	14.857308227298613
7	58.14748083906684	27.98009690157675	8.821030816195073	5.051391443161344
8	76.8388427823262	7.498202933186455	9.97582128287232	5.687133001615026
9	70.54677508191824	8.474687030312083	11.912919276692277	9.0656186110774
10	38.89972833951026	27.8101924028417	18.897674548866213	14.392404708781822
11	29.896656988956206	25.908568974691697	25.387652984064452	18.80712105228764
12	28.987387858363128	22.029705281042578	29.665138770899652	19.31776808969464
13	23.432817707409516	25.27842866344906	31.193345718313275	20.095407910828143
14	18.918212455306715	29.69407854815672	27.824195520869317	23.56351347566725
15	18.250730495990442	26.797300198844276	35.67621056955348	19.275758735611795
16	19.95257610694648	26.540576368338016	31.391256453103555	22.115591071611945
17	20.058066262754508	26.10741325068382	28.698923626994276	25.1355968595674
18	20.9197247920537	27.88954340499818	29.016327635620197	22.174404167327925
19	23.04073040263632	28.17520701276151	26.225039442115776	22.559023142486392
20	25.05998002221828	26.689009419430725	28.93791017466556	19.313100383685434
21	23.5373743220157	28.627040954452525	27.44704487532557	20.3885398482062
22	20.839440248695375	26.449089330557605	27.026951334497145	25.684519086249875
23	21.9344840784548	27.834464474089565	28.34791213510208	21.88313931235355
24	22.88389548072704	24.49425405390267	28.672784473342734	23.949065992027556
25	20.988806840989927	29.134887368254	26.15782447558323	23.718481315172845
26	20.003920873047733	29.104080508593245	26.707680243467546	24.184318374891475
27	23.412279800969017	28.00716959643014	25.87122732661806	22.709323275982783
28	18.425302700734697	28.08465351618294	30.108570841774103	23.381472941308264
29	21.134439268477117	26.99987863964376	27.49745610022498	24.368225991654143
30	20.89265209720031	26.03553057814207	27.953024206723363	25.118793117934263
31	21.7953864393805	26.618993829292652	26.79916728124796	24.786452450078883
32	23.63352906580532	28.583164517965997	25.372716324834997	22.410590091393683
33	21.18951819938573	25.878695656232786	28.1089255874308	24.822860556950683
34	20.08793958121342	25.825483807727856	27.908214229035	26.178362382023728
35	22.08011650594199	24.482118018278737	26.619927370494494	26.817838105284775
36	20.962667687338378	25.853490043783083	29.43081992923758	23.75302233964096
37	21.267002119138528	28.065982692146118	28.104257881421596	22.562757307293758
38	20.69754198601555	27.26593788216843	28.92577413904163	23.110745992774394
39	22.681317039927556	25.142131647980282	28.70079070939796	23.4757606026942
40	23.889319355109738	23.91265788515576	30.48665502851968	21.711367731214818
41	19.876959269597364	25.972983317618727	28.580363894360477	25.569693518423435
42	21.89247472437196	24.18618545729516	29.962938414286917	23.95840140404597
43	22.914702340387795	25.0011669265023	27.822328438465632	24.261802294644276
44	21.600276328195744	25.747066346773217	28.27602946256033	24.37662786247071
45	20.648997843519822	25.350311335990817	26.951334497148032	27.04935632334133
46	22.233217263043905	27.1557800203512	26.80196790485348	23.809034811751417
47	20.81143401264015	24.09003071350554	31.125197210578886	23.973338063275424
48	21.560134056516585	24.372893697663347	29.05927053090488	25.007701714915186
49	19.860155527964228	24.054556147835584	31.687189014087135	24.398099310113054
50	18.84446270036128	23.79223107011828	30.82086277877874	26.542443450741697
51	19.59409628543956	24.1096350787442	28.01183730243934	28.284431333376897
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	132.5
2	221.0
3	373.5
4	526.0
5	389.0
6	252.0
7	245.0
8	238.0
9	230.0
10	222.0
11	207.5
12	193.0
13	208.0
14	223.0
15	231.0
16	239.0
17	232.5
18	226.0
19	258.0
20	290.0
21	384.5
22	479.0
23	565.5
24	652.0
25	763.0
26	1042.0
27	1210.0
28	1412.0
29	1614.0
30	1970.0
31	2326.0
32	2450.0
33	2574.0
34	2728.0
35	2882.0
36	3191.5
37	3501.0
38	3829.5
39	4158.0
40	4597.5
41	5037.0
42	5466.5
43	5896.0
44	6861.0
45	7826.0
46	9664.0
47	11502.0
48	10723.0
49	9944.0
50	9399.0
51	8854.0
52	7795.5
53	6737.0
54	6048.5
55	5360.0
56	4984.5
57	4609.0
58	4504.5
59	4400.0
60	4276.5
61	4153.0
62	3820.0
63	3487.0
64	2820.5
65	2154.0
66	1767.0
67	1380.0
68	1176.0
69	972.0
70	840.0
71	708.0
72	593.0
73	478.0
74	378.5
75	232.0
76	185.0
77	149.0
78	113.0
79	85.0
80	57.0
81	37.5
82	18.0
83	16.5
84	15.0
85	9.5
86	4.0
87	3.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	107119.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.947833717641124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.03572583833518	37.42099907579421
2	10.51827624627107	10.928033308750082
3	5.633828127807929	8.779955003314072
4	3.4665564461057397	7.203203913404718
5	2.4637889515868165	6.399424938619666
6	1.7809006936707041	5.550835986146248
7	1.2166193437084427	4.42405175552423
8	0.8859576609280093	3.68188650006068
9	0.5912374654063185	2.764215498651033
>10	1.3621823671063509	9.305538699950521
>50	0.023361966718182796	0.8075131395924159
>100	0.017970743629371382	1.628095856010605
>500	0.0035941487258742766	1.1062463241815177
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCG	636	0.5937322043708398	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	549	0.5125141198106778	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGC	432	0.40328979919528746	No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	238	0.2221828060381445	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	224	0.20911322921237127	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCT	142	0.13256285066141393	No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	132	0.1232274386430045	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	131	0.12229389744116356	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCG	121	0.11295848542275412	No Hit
GAATCTATCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	113	0.10549015580802658	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.335412018409432E-4	0.0	0.0	0.21284739401973507	0.0
2	9.335412018409432E-4	0.0	0.0	1.087575500144699	0.0
3	9.335412018409432E-4	0.0	0.0	1.3751061903117094	0.0
4	9.335412018409432E-4	0.0	0.0	1.8474780384432268	0.0
5	9.335412018409432E-4	0.0	0.0	3.569861555839767	0.0
6	9.335412018409432E-4	0.0	0.0	4.143989394971947	0.0
7	9.335412018409432E-4	0.0	0.0	4.947768369756999	0.0
8	9.335412018409432E-4	0.0	0.0	6.202447745031227	0.0
9	9.335412018409432E-4	0.0	0.0	6.66081647513513	0.0
10	9.335412018409432E-4	0.0	0.0	8.541901996844631	0.0
11	9.335412018409432E-4	0.0	0.0	9.713496205155014	0.0
12	9.335412018409432E-4	0.0	0.0	11.26224105900914	0.0
13	9.335412018409432E-4	0.0	0.0	11.726211036324088	0.0
14	9.335412018409432E-4	0.0	0.0	11.909185111884913	0.0
15	9.335412018409432E-4	0.0	0.0	12.337680523529906	0.0
16	9.335412018409432E-4	0.0	0.0	12.991159364818566	0.0
17	9.335412018409432E-4	0.0	0.0	13.699717137015842	0.0
18	9.335412018409432E-4	0.0	0.0	14.46988862853462	0.0
19	9.335412018409432E-4	0.0	0.0	14.90585236979434	0.0
20	9.335412018409432E-4	0.0	0.0	15.344616734659585	0.0
21	9.335412018409432E-4	0.0	0.0	15.866466266488672	0.0
22	9.335412018409432E-4	0.0	0.0	16.41165432836378	0.0
23	9.335412018409432E-4	0.0	0.0	16.929769695385506	0.0
24	9.335412018409432E-4	0.0	0.0	17.35079677741577	0.0
25	9.335412018409432E-4	0.0	0.0	17.727947422959513	0.0
26	9.335412018409432E-4	0.0	0.0	18.086427244466435	0.0
27	9.335412018409432E-4	0.0	0.0	18.44117290116599	0.0
28	9.335412018409432E-4	0.0	0.0	18.780048357434254	0.0
29	9.335412018409432E-4	0.0	0.0	19.13386047293197	0.0
30	9.335412018409432E-4	0.0	0.0	19.57635900260458	0.0
31	9.335412018409432E-4	0.0	0.0	19.94230715372623	0.0
32	9.335412018409432E-4	0.0	0.0	20.31945779926997	0.0
33	9.335412018409432E-4	0.0	0.0	20.630327019483005	0.0
34	9.335412018409432E-4	0.0	0.0	20.953332275319973	0.0
35	9.335412018409432E-4	0.0	0.0	21.3006096024048	0.0
36	9.335412018409432E-4	0.0	0.0	21.647886929489633	0.0
37	9.335412018409432E-4	0.0	0.0	22.015702163014964	0.0
38	9.335412018409432E-4	0.0	0.0	22.32843846563168	0.0
39	9.335412018409432E-4	0.0	0.0	22.698120781560693	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGAT	30	2.1470478E-6	45.000004	8
GCACGGG	35	1.1981865E-7	45.000004	3
AATGAAT	30	2.1470478E-6	45.000004	2
TCTATCT	70	0.0	45.000004	4
AGCGTGG	20	7.00464E-4	45.0	2
ATGGGAT	40	6.715709E-9	45.0	5
CTCACGA	20	7.00464E-4	45.0	24
ACACGGG	25	3.8670794E-5	45.0	3
ATAGGTC	20	7.00464E-4	45.0	5
CGACGGT	20	7.00464E-4	45.0	28
GGCACGG	20	7.00464E-4	45.0	2
CACGACG	20	7.00464E-4	45.0	26
CTACGAA	20	7.00464E-4	45.0	11
TACGAAT	20	7.00464E-4	45.0	12
CGAATAT	20	7.00464E-4	45.0	14
CGGGACC	20	7.00464E-4	45.0	6
CGGGAAC	25	3.8670794E-5	45.0	6
CATACGA	45	3.783498E-10	45.0	18
CGGTCTA	20	7.00464E-4	45.0	31
GCTACGA	20	7.00464E-4	45.0	10
>>END_MODULE