##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936325.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	164052
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.980658571672397	33.0	31.0	34.0	30.0	34.0
2	32.08944724843342	33.0	31.0	34.0	30.0	34.0
3	31.979762514324726	33.0	31.0	34.0	30.0	34.0
4	35.74792748640675	37.0	35.0	37.0	35.0	37.0
5	35.80839611830395	37.0	35.0	37.0	35.0	37.0
6	35.78509253163631	37.0	35.0	37.0	35.0	37.0
7	26.77585765488991	35.0	10.0	37.0	0.0	37.0
8	31.09534172091776	35.0	19.0	37.0	17.0	37.0
9	35.84987686831005	39.0	32.0	39.0	32.0	39.0
10	36.63932167849219	38.0	35.0	39.0	33.0	39.0
11	37.00808280301368	39.0	37.0	39.0	34.0	39.0
12	37.04894789457001	39.0	37.0	39.0	34.0	39.0
13	37.00469363372589	39.0	37.0	39.0	33.0	39.0
14	38.218644088459754	40.0	37.0	41.0	33.0	41.0
15	38.26589130275766	40.0	37.0	41.0	34.0	41.0
16	38.18598371248141	40.0	37.0	41.0	33.0	41.0
17	38.06435764269866	40.0	37.0	41.0	33.0	41.0
18	37.935508253480606	40.0	37.0	41.0	33.0	41.0
19	37.84649989027869	40.0	37.0	41.0	33.0	41.0
20	37.89869065905932	40.0	36.0	41.0	33.0	41.0
21	37.85024260600297	40.0	36.0	41.0	33.0	41.0
22	37.86908419281691	40.0	36.0	41.0	33.0	41.0
23	37.830572013751734	40.0	36.0	41.0	33.0	41.0
24	37.80856679589399	40.0	36.0	41.0	33.0	41.0
25	37.71171945480701	40.0	36.0	41.0	33.0	41.0
26	37.548850364518565	40.0	36.0	41.0	33.0	41.0
27	37.42294516372857	39.0	36.0	41.0	33.0	41.0
28	37.392491405164215	39.0	36.0	41.0	32.0	41.0
29	37.332528710408894	39.0	36.0	41.0	32.0	41.0
30	37.143149733011484	39.0	35.0	41.0	32.0	41.0
31	37.07554921610221	39.0	35.0	41.0	31.0	41.0
32	36.899489186355545	39.0	35.0	41.0	31.0	41.0
33	36.72438007461049	39.0	35.0	41.0	30.0	41.0
34	36.63821227415698	39.0	35.0	41.0	30.0	41.0
35	36.449497720235044	39.0	35.0	41.0	30.0	41.0
36	36.28663472557482	39.0	35.0	41.0	30.0	41.0
37	36.2220576402604	39.0	35.0	41.0	30.0	41.0
38	36.09378733572282	39.0	35.0	41.0	29.0	41.0
39	36.06926462341209	39.0	35.0	40.0	29.0	41.0
40	35.78881696047595	39.0	35.0	40.0	27.0	41.0
41	35.710683198010386	38.0	35.0	40.0	27.0	41.0
42	35.656310194328626	38.0	35.0	40.0	27.0	41.0
43	35.57817643186307	38.0	35.0	40.0	27.0	41.0
44	35.40408529002999	38.0	34.0	40.0	26.0	41.0
45	35.291108916684955	38.0	34.0	40.0	26.0	41.0
46	35.077201131348595	38.0	34.0	40.0	25.0	41.0
47	34.92124448345646	38.0	34.0	40.0	24.0	41.0
48	34.82461658498525	38.0	34.0	40.0	23.0	41.0
49	34.627886279960016	38.0	34.0	40.0	23.0	41.0
50	34.40905322702558	37.0	33.0	40.0	23.0	41.0
51	32.14973910711238	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	16.0
10	16.0
11	14.0
12	11.0
13	6.0
14	6.0
15	11.0
16	28.0
17	55.0
18	72.0
19	127.0
20	231.0
21	363.0
22	537.0
23	837.0
24	1109.0
25	1452.0
26	1841.0
27	2236.0
28	2422.0
29	2913.0
30	3481.0
31	4357.0
32	5589.0
33	7480.0
34	11717.0
35	14007.0
36	15365.0
37	24262.0
38	30584.0
39	32896.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.458464389339966	19.4164045546534	23.617511520737327	12.507619535269304
2	31.15963231170604	27.15907151391022	25.10179699119791	16.57949918318582
3	31.820398410260157	26.92682807890181	25.45961036744447	15.793163143393556
4	29.189525272474583	25.529710091922077	26.765903494014093	18.514861141589254
5	26.789676444054322	28.99507473240192	24.052129812498478	20.16311901104528
6	25.843634945017435	34.85845951283739	24.931119401165482	14.36678614097969
7	59.24706800282837	28.44098212761807	7.850559578670177	4.4613902908833785
8	79.59000804622924	6.9953429400434	8.856338234218418	4.558310779508936
9	72.89030307463487	8.249213663960207	10.951405651866482	7.909077609538439
10	41.473435252237095	25.62236364079682	18.264940384756052	14.639260722210032
11	31.999000316972666	26.19413356740546	23.71930851193524	18.087557603686637
12	31.427839953185575	22.583692975398044	27.121888181796017	18.866578889620364
13	25.683929485772804	23.91558774047253	29.076146587667324	21.32433618608734
14	20.593470363055616	27.967961378099627	28.337966010777073	23.100602248067688
15	20.526418452685736	26.7055567746812	33.628971301782364	19.139053470850705
16	23.434642674274013	25.54190134835296	30.86582303172165	20.15763294565138
17	23.261526832955404	25.050593714188185	28.71040889474069	22.97747055811572
18	22.9524784824324	27.48762587472265	28.53241655084973	21.02747909199522
19	25.507765830346475	28.188623119498697	25.74732401921342	20.55628703094141
20	27.292565771828443	26.561090385975177	27.668666032721333	18.477677809475043
21	25.948479750323067	27.643064394216466	26.937190646868064	19.4712652085924
22	23.563869992441422	26.222173457196497	27.194426157559796	23.01953039280228
23	24.37093116816619	26.742740106795402	28.32699387998927	20.559334845049133
24	24.810425962499696	23.91558774047253	28.62324141125984	22.650744885767928
25	23.02196864408846	27.02252944188428	26.538536561578034	23.416965352449225
26	21.96803452563821	27.81800892399971	26.756150488869384	23.457806061492697
27	22.3386487211372	28.37941628264209	27.160290639553313	22.121644356667396
28	20.538000146295076	28.297125301733594	28.90851681174262	22.256357740228708
29	25.099358739911736	27.188330529344356	26.500743666642286	21.211567064101626
30	23.389535025479727	25.661985224197203	29.276083193133886	21.672396557189185
31	24.170994562699633	25.877770463023918	26.777485187623434	23.173749786653012
32	27.329749103942653	26.506839294857727	26.57389120522761	19.58952039597201
33	24.128325165191526	26.79333382098359	27.458366859288518	21.61997415453637
34	23.826591568527054	26.06063930948724	29.761904761904763	20.35086436008095
35	23.81561943773925	25.638821836978522	28.542779118815986	22.00277960646624
36	22.8439763001975	29.45041816009558	26.842708409528687	20.862897130178236
37	23.951551946943653	28.665301245946406	27.479701558042574	19.90344524906737
38	23.051227659522592	27.43459390924829	26.35322946382854	23.16094896740058
39	25.28222758637505	25.751590958964233	27.18772096652281	21.778460488137906
40	25.32489698388316	23.716870260649063	29.921610221149393	21.036622534318386
41	20.34111135493624	27.834467120181404	28.89022992709629	22.934191597786068
42	22.822641601443443	26.11245214931851	28.394045790359158	22.67086045887889
43	23.70955550679053	26.45258820374028	26.622656230951165	23.215200058518032
44	22.782410455221516	25.714407626850022	27.43459390924829	24.068588008680177
45	22.67878477555897	25.176773218247874	27.03715894960135	25.10728305659181
46	23.06341891595348	27.387048009167824	26.670202131031623	22.879330943847073
47	21.932070319167092	25.482164191841612	30.068514861141587	22.517250627849705
48	22.40082412893473	24.501987174798234	29.464438104991103	23.632750591275936
49	21.408455855460463	25.22614780679297	29.662546021993023	23.70285031575354
50	20.36732255626265	25.298685782556753	29.23889986101968	25.095091800160922
51	20.425231024309365	24.26242898593129	28.264818472191745	27.047521517567603
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	235.0
2	368.0
3	655.5
4	943.0
5	662.0
6	381.0
7	349.0
8	317.0
9	323.0
10	329.0
11	351.5
12	374.0
13	371.0
14	368.0
15	384.0
16	400.0
17	407.5
18	415.0
19	449.0
20	483.0
21	606.5
22	730.0
23	815.0
24	900.0
25	1072.5
26	1460.5
27	1676.0
28	2031.0
29	2386.0
30	2754.5
31	3123.0
32	3525.5
33	3928.0
34	4522.5
35	5117.0
36	5339.5
37	5562.0
38	5968.5
39	6375.0
40	6971.5
41	7568.0
42	8220.0
43	8872.0
44	9676.0
45	10480.0
46	12546.0
47	14612.0
48	13961.0
49	13310.0
50	12833.0
51	12356.0
52	11469.0
53	10582.0
54	9771.5
55	8961.0
56	8719.5
57	8478.0
58	8348.0
59	8218.0
60	7972.0
61	7726.0
62	7031.0
63	6336.0
64	5324.5
65	4313.0
66	3440.0
67	2567.0
68	2109.0
69	1651.0
70	1277.5
71	904.0
72	786.5
73	669.0
74	539.5
75	315.5
76	221.0
77	173.0
78	125.0
79	98.0
80	71.0
81	56.0
82	41.0
83	29.5
84	18.0
85	15.5
86	13.0
87	12.0
88	11.0
89	12.0
90	13.0
91	7.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	164052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.3380147758028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.3254262416605	34.20378904249872
2	11.055102545095131	10.908736254358374
3	5.964912280701754	8.828907907248922
4	3.943661971830986	7.782898105478751
5	2.7575982209043737	6.802721088435375
6	2.023721275018532	5.990783410138248
7	1.441808747220163	4.979518689196109
8	1.0415122312824314	4.110891668495356
9	0.7635285396590067	3.390388413429888
>10	1.653076352853966	10.486309219028113
>50	0.012354830738818878	0.4401043571550484
>100	0.014825796886582655	1.3257991368590447
>500	0.002470966147763776	0.7491527076780533
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC	628	0.3828054519298759	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG	601	0.3663472557481774	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC	486	0.29624753127057274	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	382	0.23285299782995636	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	215	0.1310560066320435	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.114597810450345	0.0
2	0.0	0.0	0.0	0.5943237510057786	0.0
3	0.0	0.0	0.0	0.7887742910784385	0.0
4	0.0	0.0	0.0	1.1014800185307099	0.0
5	0.0	0.0	0.0	2.259039816643503	0.0
6	0.0	0.0	0.0	2.7472996367005584	0.0
7	0.0	0.0	0.0	3.1867944310340626	0.0
8	0.0	0.0	0.0	3.8774291078438545	0.0
9	0.0	0.0	0.0	4.120035110818521	0.0
10	6.095628215443884E-4	0.0	0.0	5.256869772998805	0.0
11	6.095628215443884E-4	0.0	0.0	6.107209909053227	0.0
12	6.095628215443884E-4	0.0	0.0	7.267817521273742	0.0
13	6.095628215443884E-4	0.0	0.0	7.614049203910955	0.0
14	6.095628215443884E-4	0.0	0.0	7.778021602906396	0.0
15	6.095628215443884E-4	0.0	0.0	8.085850827786311	0.0
16	6.095628215443884E-4	0.0	0.0	8.637505181283982	0.0
17	6.095628215443884E-4	0.0	0.0	9.186111720673933	0.0
18	6.095628215443884E-4	0.0	0.0	9.79689366786141	0.0
19	6.095628215443884E-4	0.0	0.0	10.171774803111209	0.0
20	6.095628215443884E-4	0.0	0.0	10.563114134542706	0.0
21	6.095628215443884E-4	0.0	0.0	11.019067125057909	0.0
22	6.095628215443884E-4	0.0	0.0	11.526832955404384	0.0
23	6.095628215443884E-4	0.0	0.0	11.982176383098041	0.0
24	6.095628215443884E-4	0.0	0.0	12.360105332455563	0.0
25	6.095628215443884E-4	0.0	0.0	12.682564065052544	0.0
26	6.095628215443884E-4	0.0	0.0	13.008070611757248	0.0
27	6.095628215443884E-4	0.0	0.0	13.323214590495697	0.0
28	6.095628215443884E-4	0.0	0.0	13.667617584668276	0.0
29	6.095628215443884E-4	0.0	0.0	13.977275498012824	0.0
30	6.095628215443884E-4	0.0	0.0	14.333260185794748	0.0
31	6.095628215443884E-4	0.0	0.0	14.681320556896594	0.0
32	6.095628215443884E-4	0.0	0.0	15.036695681856973	0.0
33	6.095628215443884E-4	0.0	0.0	15.384146490137274	0.0
34	6.095628215443884E-4	0.0	0.0	15.738912052276108	0.0
35	6.095628215443884E-4	0.0	0.0	16.071733352839345	0.0
36	6.095628215443884E-4	0.0	0.0	16.41186940726111	0.0
37	6.095628215443884E-4	0.0	0.0	16.7660254065784	0.0
38	6.095628215443884E-4	0.0	0.0	17.11042840075098	0.0
39	6.095628215443884E-4	0.0	0.0	17.4938434155024	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGAT	20	7.015597E-4	45.0	8
ACGGGCG	20	7.015597E-4	45.0	5
ATCGTTG	25	3.8761493E-5	45.0	23
CTGCATA	20	7.015597E-4	45.0	11
GCGATTC	20	7.015597E-4	45.0	8
ATGCGCG	20	7.015597E-4	45.0	1
AAAGCCG	20	7.015597E-4	45.0	27
ACGAAGA	20	7.015597E-4	45.0	33
ACAACGA	30	2.1540982E-6	44.999996	13
GAGCGAC	30	2.1540982E-6	44.999996	18
TTACACG	55	1.8189894E-12	44.999996	34
ACAAGCG	65	0.0	41.538464	14
ATGGGAC	60	3.6379788E-12	41.249996	5
GTCTTAC	60	3.6379788E-12	41.249996	31
AACGGGA	50	1.0695658E-9	40.5	4
CGGTAGT	50	1.0695658E-9	40.5	12
TCCGCCC	45	1.9110303E-8	40.0	35
ACGGGAT	45	1.9110303E-8	40.0	5
CGCCCAA	45	1.9110303E-8	40.0	37
GGGTACC	40	3.4341065E-7	39.375	7
>>END_MODULE