##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936323.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71376
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02381752970186	33.0	31.0	34.0	30.0	34.0
2	32.141364043936335	33.0	31.0	34.0	30.0	34.0
3	32.027516251961444	33.0	31.0	34.0	30.0	34.0
4	35.77778244788164	37.0	35.0	37.0	35.0	37.0
5	35.81817417619368	37.0	35.0	37.0	35.0	37.0
6	35.78055648957633	37.0	35.0	37.0	35.0	37.0
7	26.611059739968617	35.0	10.0	37.0	0.0	37.0
8	30.95715646715983	35.0	19.0	37.0	17.0	37.0
9	35.79931629679444	38.0	32.0	39.0	32.0	39.0
10	36.68139150414705	38.0	35.0	39.0	33.0	39.0
11	36.99463405066129	39.0	37.0	39.0	34.0	39.0
12	37.01333781663304	39.0	37.0	39.0	33.0	39.0
13	36.87127325711724	39.0	37.0	39.0	33.0	39.0
14	38.194126877381755	40.0	37.0	41.0	33.0	41.0
15	38.17864548307554	40.0	37.0	41.0	33.0	41.0
16	38.22866229544945	40.0	37.0	41.0	34.0	41.0
17	38.044398677426585	40.0	37.0	41.0	33.0	41.0
18	37.945373795113206	40.0	37.0	41.0	33.0	41.0
19	37.807162071284466	40.0	36.0	41.0	33.0	41.0
20	37.89151815736382	40.0	36.0	41.0	33.0	41.0
21	37.90833053127101	40.0	36.0	41.0	33.0	41.0
22	37.902698386012105	40.0	36.0	41.0	33.0	41.0
23	37.907742098184265	40.0	36.0	41.0	33.0	41.0
24	37.82276955839498	40.0	36.0	41.0	33.0	41.0
25	37.74913136068146	40.0	36.0	41.0	33.0	41.0
26	37.54867182246133	40.0	36.0	41.0	33.0	41.0
27	37.49936953597848	39.0	36.0	41.0	33.0	41.0
28	37.49295281327057	40.0	36.0	41.0	33.0	41.0
29	37.43108327729209	40.0	36.0	41.0	33.0	41.0
30	37.37019446312486	40.0	36.0	41.0	33.0	41.0
31	37.29659829634611	40.0	35.0	41.0	32.0	41.0
32	37.16512553239184	39.0	35.0	41.0	32.0	41.0
33	37.01884386908765	39.0	35.0	41.0	31.0	41.0
34	36.90075095270119	39.0	35.0	41.0	31.0	41.0
35	36.86034521407756	39.0	35.0	41.0	31.0	41.0
36	36.65579466487335	39.0	35.0	41.0	31.0	41.0
37	36.625714525891055	39.0	35.0	41.0	31.0	41.0
38	36.43245628782784	39.0	35.0	41.0	30.0	41.0
39	36.318328289621164	39.0	35.0	41.0	30.0	41.0
40	35.994437906299034	39.0	35.0	40.0	29.0	41.0
41	35.95592355973997	39.0	35.0	40.0	29.0	41.0
42	35.90564055144586	39.0	35.0	40.0	29.0	41.0
43	35.85810356422327	39.0	35.0	40.0	28.0	41.0
44	35.65199787043264	38.0	35.0	40.0	27.0	41.0
45	35.52729208697601	38.0	35.0	40.0	27.0	41.0
46	35.21213012777404	38.0	34.0	40.0	26.0	41.0
47	34.97783568706568	38.0	34.0	40.0	24.0	41.0
48	34.95843140551446	38.0	34.0	40.0	24.0	41.0
49	34.75627661959202	38.0	34.0	40.0	24.0	41.0
50	34.65079298363596	37.0	34.0	40.0	24.0	41.0
51	32.43415153553015	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	10.0
11	6.0
12	3.0
13	3.0
14	5.0
15	5.0
16	9.0
17	23.0
18	44.0
19	65.0
20	100.0
21	134.0
22	184.0
23	260.0
24	424.0
25	529.0
26	710.0
27	859.0
28	1075.0
29	1241.0
30	1518.0
31	1864.0
32	2436.0
33	3314.0
34	5020.0
35	6288.0
36	6457.0
37	10368.0
38	13392.0
39	15017.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.504091010984084	20.08938578793992	22.472539789284916	11.933983411791079
2	29.816745124411565	29.61219457520735	23.338377045505492	17.23268325487559
3	31.59185160277965	28.298027348128223	24.8725061645371	15.23761488455503
4	29.043375924680564	24.02067921990585	28.935496525442726	18.000448329970858
5	27.88612418740193	29.09241201524322	23.405626541134275	19.615837256220576
6	25.2549876709258	37.87547635059404	23.842748262721365	13.026787715758797
7	57.63001569154898	28.70432638421879	9.379903609056266	4.28575431517597
8	77.26967047747142	9.88567585743107	8.715814839722036	4.128838825375476
9	69.97870432638422	10.114043936337143	10.031383097960099	9.875868639318538
10	41.51815736381977	26.656018829858773	18.206399910334007	13.619423895987449
11	33.753642681013226	25.353059852051107	23.767092580138982	17.126204886796685
12	33.872730329522525	21.837872674288274	26.13903833221251	18.150358663976686
13	24.602107150863034	25.107879399237838	30.094149293880296	20.195864156018832
14	20.17064559515804	29.662631696928937	26.111017709033852	24.055704998879175
15	19.321620712844652	26.913808563102442	35.039789284913695	18.724781439139207
16	22.005996413360233	25.652880520062766	30.70359784801614	21.63752521856086
17	21.41055817081372	26.458473436449225	29.20589553911679	22.925072853620264
18	21.17238287379511	28.861241874019278	27.601714862138532	22.364660390047074
19	25.29982066801166	28.70852947769558	24.690372113875814	21.301277740416946
20	26.642008518269446	27.02308899349922	29.449674960771127	16.885227527460213
21	25.334846446984983	29.201692445639992	25.86023313158485	19.603227975790183
22	22.59442950011208	26.30576104012553	26.80873122618247	24.291078233579917
23	23.463068818650527	29.07419861017709	27.624131360681464	19.83860121049092
24	23.900190540237613	24.37794216543376	28.41991705895539	23.301950235373234
25	22.049428379287157	30.151591571396548	25.910670253306435	21.888309796009864
26	20.890775610849584	29.247926473884778	26.20768885900022	23.653609056265413
27	23.977247253978927	30.178211163416275	25.552006276619593	20.292535305985204
28	20.111802286482853	29.33899349921542	29.302566689083164	21.246637525218564
29	23.551333781663303	28.98453261600538	26.731674512441156	20.73245908989016
30	23.552734812822237	29.44266980497646	26.734476574759025	20.270118807442277
31	25.81960322797579	28.72113875812598	23.638197713517147	21.82106030038108
32	27.52465814839722	29.081203765971754	23.509302846895313	19.88483523873571
33	24.17899574086528	29.976462676529923	23.53031831427931	22.314223268325488
34	23.790910109840844	30.79186281102892	25.291414481058062	20.125812598072184
35	25.984924904729883	26.09140327280879	24.625924680564896	23.297747141896437
36	23.80772248374804	31.09028244788164	23.39862138533961	21.703373683030712
37	26.31977135171486	29.270342972427706	26.104012553239187	18.305873122618248
38	24.808058731226183	28.477359336471643	24.230833893745796	22.483748038556378
39	25.828009414929387	27.907139654785922	26.019950683703208	20.244900246581484
40	25.288612418740193	28.069659269222146	26.839553911678998	19.802174400358663
41	20.21968168572069	29.388029589778075	27.055312710154674	23.336976014346558
42	22.898453261600537	26.85356422326833	27.810468504819546	22.43751401031159
43	24.775835014570724	26.569154898004932	24.691773145034745	23.9632369423896
44	23.215086303519392	27.315904505716208	26.30996413360233	23.15904505716207
45	22.576216095045954	27.470017933198836	24.94816184711948	25.005604124635735
46	23.210883210042592	31.240192781887473	23.729264738847792	21.819659269222146
47	21.762216991705895	26.612586863931853	30.535474108944182	21.089722035418067
48	23.376204886796685	26.329578569827394	27.037099305088546	23.257117238287382
49	20.65820443846671	26.930620937009643	29.815344093252634	22.595830531271016
50	20.64979825151311	26.829746693566463	28.560020174848688	23.96043488007173
51	21.587088096839274	26.087200179331987	25.982122842412014	26.343588881416725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	49.0
1	103.5
2	158.0
3	326.0
4	494.0
5	353.5
6	213.0
7	187.0
8	161.0
9	156.0
10	151.0
11	150.5
12	150.0
13	155.0
14	160.0
15	145.0
16	130.0
17	139.0
18	148.0
19	173.5
20	199.0
21	250.0
22	301.0
23	353.5
24	406.0
25	473.5
26	696.0
27	851.0
28	999.0
29	1147.0
30	1401.5
31	1656.0
32	1906.0
33	2156.0
34	2317.5
35	2479.0
36	2451.5
37	2424.0
38	2601.5
39	2779.0
40	3072.0
41	3365.0
42	3612.5
43	3860.0
44	4112.5
45	4365.0
46	4617.0
47	4869.0
48	6017.5
49	7166.0
50	6107.0
51	5048.0
52	4832.5
53	4617.0
54	4239.0
55	3861.0
56	3610.5
57	3360.0
58	3387.0
59	3414.0
60	3466.5
61	3519.0
62	3017.0
63	2515.0
64	2129.0
65	1743.0
66	1410.5
67	1078.0
68	899.0
69	720.0
70	599.0
71	478.0
72	372.5
73	267.0
74	219.0
75	130.0
76	89.0
77	66.0
78	43.0
79	41.5
80	40.0
81	26.0
82	12.0
83	11.0
84	10.0
85	7.5
86	5.0
87	5.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	71376.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.37760591795561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.06921517520996	32.44648060972876
2	12.400810889081958	11.998430845101996
3	6.689834926151173	9.709145931405514
4	4.610483637416738	8.921766420085183
5	3.006081668114683	7.2713517148621385
6	2.082247321169997	6.0440484196368525
7	1.3582392122791775	4.599585294776956
8	0.87460179554011	3.384891279982067
9	0.6805676223573703	2.9631809011432413
>10	1.175789168838691	7.830363147276395
>50	0.020272227048942947	0.7061197041022192
>100	0.026064291920069507	2.1912127325711723
>500	0.005792064871126557	1.933422999327505
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG	704	0.9863259358888142	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGC	676	0.9470970634386908	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC	442	0.6192557722483748	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	174	0.24377942165433758	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGC	155	0.21715982963461106	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	153	0.21435776731674513	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG	150	0.21015467383994618	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCT	137	0.19194126877381754	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	124	0.17372786370768886	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTT	119	0.166722707913024	No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC	110	0.1541134274826272	Illumina PCR Primer Index 8 (95% over 23bp)
GAATCTATCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC	93	0.13029589778076667	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	88	0.12329074198610178	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTC	78	0.10928043039677203	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	78	0.10928043039677203	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06444743331091683	0.0
2	0.0	0.0	0.0	0.4903609056265411	0.0
3	0.0	0.0	0.0	0.5772248374803856	0.0
4	0.0	0.0	0.0	0.8392176642008519	0.0
5	0.0	0.0	0.0	2.167395202869312	0.0
6	0.0	0.0	0.0	2.4139766868415156	0.0
7	0.0	0.0	0.0	2.8524994395875365	0.0
8	0.0	0.0	0.0	3.361073750280206	0.0
9	0.0	0.0	0.0	3.502577897332437	0.0
10	0.0	0.0	0.0	5.342131809011432	0.0
11	0.0	0.0	0.0	5.887132929836359	0.0
12	0.0	0.0	0.0	7.65803631472764	0.0
13	0.0	0.0	0.0	7.901815736381977	0.0
14	0.0	0.0	0.0	8.005492042143016	0.0
15	0.0	0.0	0.0	8.295505492042142	0.0
16	0.0	0.0	0.0	8.630351939027124	0.0
17	0.0	0.0	0.0	9.008630351939027	0.0
18	0.0	0.0	0.0	9.410726294552791	0.0
19	0.0	0.0	0.0	9.681125308226855	0.0
20	0.0	0.0	0.0	9.959930508854518	0.0
21	0.0	0.0	0.0	10.254147052230442	0.0
22	0.0	0.0	0.0	10.539957408652768	0.0
23	0.0	0.0	0.0	10.817361578121497	0.0
24	0.0	0.0	0.0	11.017709033848913	0.0
25	0.0	0.0	0.0	11.239071956960323	0.0
26	0.0	0.0	0.0	11.473044160502129	0.0
27	0.0	0.0	0.0	11.670589553911679	0.0
28	0.0	0.0	0.0	11.894754539340955	0.0
29	0.0	0.0	0.0	12.110513337816633	0.0
30	0.0	0.0	0.0	12.403328850033624	0.0
31	0.0	0.0	0.0	12.659717552118359	0.0
32	0.0	0.0	0.0	12.885283568706567	0.0
33	0.0	0.0	0.0	13.12626092804304	0.0
34	0.0	0.0	0.0	13.412071284465366	0.0
35	0.0	0.0	0.0	13.661454830755437	0.0
36	0.0	0.0	0.0	13.96968168572069	0.0
37	0.0	0.0	0.0	14.254091010984084	0.0
38	0.0	0.0	0.0	14.48245908989016	0.0
39	0.0	0.0	0.0	14.743050885451693	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAGG	20	6.9888425E-4	45.000004	3
CAACGGG	20	6.9888425E-4	45.000004	3
TTATGAC	20	6.9888425E-4	45.000004	27
GAATCTA	20	6.9888425E-4	45.000004	1
TGACGGG	20	6.9888425E-4	45.000004	3
ATCAACG	20	6.9888425E-4	45.000004	15
AAACGGG	20	6.9888425E-4	45.000004	3
GCTTATG	20	6.9888425E-4	45.000004	25
CAAGGCG	20	6.9888425E-4	45.000004	5
TTGGTAG	20	6.9888425E-4	45.000004	1
CATACGA	35	1.1906377E-7	45.000004	18
TTCGGGT	20	6.9888425E-4	45.000004	26
CGCACTT	20	6.9888425E-4	45.000004	35
ACATACG	35	1.1906377E-7	45.000004	17
CACCCAT	35	1.1906377E-7	45.000004	31
TCAACGC	20	6.9888425E-4	45.000004	16
GGGATCA	35	1.1906377E-7	45.000004	7
TGATACC	100	0.0	45.0	4
GATACCT	100	0.0	45.0	5
TAAAGGG	25	3.8540154E-5	45.0	3
>>END_MODULE