##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936322.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	147543
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0173102078716	33.0	31.0	34.0	30.0	34.0
2	32.13907132158083	33.0	31.0	34.0	30.0	34.0
3	32.03483730166799	33.0	31.0	34.0	30.0	34.0
4	35.78859722250463	37.0	35.0	37.0	35.0	37.0
5	35.853744332160794	37.0	35.0	37.0	35.0	37.0
6	35.81926624780572	37.0	35.0	37.0	35.0	37.0
7	26.863937970625514	35.0	10.0	37.0	0.0	37.0
8	31.16612784069729	35.0	19.0	37.0	17.0	37.0
9	35.9329280277614	39.0	32.0	39.0	32.0	39.0
10	36.68085913936954	38.0	35.0	39.0	33.0	39.0
11	37.04706424567753	39.0	37.0	39.0	34.0	39.0
12	37.12289976481433	39.0	37.0	39.0	34.0	39.0
13	37.084849840385516	39.0	37.0	39.0	33.0	39.0
14	38.288763275790785	40.0	38.0	41.0	34.0	41.0
15	38.36625932778919	40.0	38.0	41.0	34.0	41.0
16	38.31438292565557	40.0	37.0	41.0	34.0	41.0
17	38.20673973011258	40.0	37.0	41.0	34.0	41.0
18	38.095938133289955	40.0	37.0	41.0	33.0	41.0
19	37.989657252461996	40.0	37.0	41.0	33.0	41.0
20	38.06990504463106	40.0	37.0	41.0	34.0	41.0
21	38.03078424594864	40.0	37.0	41.0	33.0	41.0
22	38.02288146506442	40.0	37.0	41.0	33.0	41.0
23	37.95940844364016	40.0	37.0	41.0	33.0	41.0
24	37.90908413140576	40.0	36.0	41.0	33.0	41.0
25	37.81302399978311	40.0	36.0	41.0	33.0	41.0
26	37.67696874809378	40.0	36.0	41.0	33.0	41.0
27	37.58376879960419	40.0	36.0	41.0	33.0	41.0
28	37.54944660200755	40.0	36.0	41.0	33.0	41.0
29	37.50714706899006	40.0	36.0	41.0	33.0	41.0
30	37.37234568905336	40.0	36.0	41.0	32.0	41.0
31	37.25848057854321	39.0	36.0	41.0	32.0	41.0
32	37.11689473577194	39.0	35.0	41.0	31.0	41.0
33	36.99922056620782	39.0	35.0	41.0	31.0	41.0
34	36.873494506686185	39.0	35.0	41.0	31.0	41.0
35	36.81189212636316	39.0	35.0	41.0	31.0	41.0
36	36.63363222924842	39.0	35.0	41.0	30.0	41.0
37	36.61085242946124	39.0	35.0	41.0	30.0	41.0
38	36.441105304894165	39.0	35.0	41.0	30.0	41.0
39	36.429549351714414	39.0	35.0	41.0	30.0	41.0
40	36.187097998549575	39.0	35.0	40.0	29.0	41.0
41	36.14204672536142	39.0	35.0	40.0	29.0	41.0
42	36.07744860820235	39.0	35.0	40.0	29.0	41.0
43	36.052011955836605	39.0	35.0	40.0	29.0	41.0
44	35.818019153738234	38.0	35.0	40.0	28.0	41.0
45	35.68171990538352	38.0	35.0	40.0	28.0	41.0
46	35.51080701897074	38.0	34.0	40.0	27.0	41.0
47	35.36447679659489	38.0	34.0	40.0	26.0	41.0
48	35.290979578834644	38.0	34.0	40.0	26.0	41.0
49	35.118758599188034	38.0	34.0	40.0	26.0	41.0
50	34.90797259104126	38.0	34.0	40.0	26.0	41.0
51	32.71575066251872	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	10.0
10	9.0
11	6.0
12	4.0
13	3.0
14	3.0
15	8.0
16	22.0
17	29.0
18	53.0
19	86.0
20	152.0
21	237.0
22	389.0
23	553.0
24	819.0
25	1067.0
26	1394.0
27	1633.0
28	1999.0
29	2300.0
30	2884.0
31	3675.0
32	4734.0
33	6639.0
34	10267.0
35	12853.0
36	14057.0
37	22355.0
38	28757.0
39	30535.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6645588065852	20.49843096588791	23.87371817029612	11.963292057230774
2	31.483025287543292	24.87342672983469	25.52069566160374	18.12285232101828
3	32.37225757914642	25.61422771666565	25.90227933551575	16.111235368672183
4	29.346021159933038	25.071335136197582	26.30216275933118	19.280480944538205
5	25.847380085805494	29.177256799712627	24.28037927926096	20.694983835220917
6	26.565136943128444	35.1890635272429	23.9733501419925	14.272449387636147
7	60.500328717729744	28.224314267705008	6.95119388923907	4.3241631253261765
8	80.93776051727292	6.631287150186725	8.193543577126668	4.237408755413676
9	73.88286804524783	9.035332072683895	10.098073104112022	6.983726777956257
10	40.58748974875121	28.39511193347024	17.104844011576287	13.91255430620226
11	30.580237625641338	26.222863843083033	25.330920477420143	17.865978053855486
12	29.99261232318714	22.947886378886157	28.131459981158034	18.92804131676867
13	25.04490216411487	24.332567454911448	29.600862121551007	21.021668259422675
14	19.777285265990255	27.387270151752368	28.875649810563704	23.959794771693677
15	19.56311041526877	27.09718522735745	33.48244240662044	19.85726195075334
16	22.629335176863695	25.699626549548267	30.809323383691535	20.861714889896504
17	22.74794466697844	25.608805568546117	28.49067729407698	23.15257247039846
18	23.08547338741926	26.19643087100032	29.181323410802275	21.536772330778145
19	24.767694841503832	27.844086130822877	26.344184407257544	21.044034620415744
20	26.05681055692239	26.060199399497098	28.15721518472581	19.725774858854706
21	25.45088550456477	27.075496634879325	27.779020353388507	19.6945975071674
22	24.041804762001586	24.931714822119652	26.954853839219755	24.071626576659007
23	23.271859729028147	27.428614031163796	28.298191035833618	21.001335203974435
24	23.655476708484986	24.14550334478762	29.488352548070733	22.71066739865666
25	22.33518364137912	27.151406708552763	28.092149407291434	22.421260242776683
26	22.301972984146996	28.578109432504423	26.909443348718682	22.210474234629903
27	22.84012118501047	28.43238920179202	26.999586561205884	21.727903051991625
28	20.75801630711047	27.556034511972783	29.350765539537626	22.33518364137912
29	23.42639095043479	26.319106972204715	28.8871718753177	21.367330202042794
30	23.941495021790256	26.324529120324247	28.524565719824054	21.209410138061447
31	24.013338484374046	27.646855492974932	26.504137776783715	21.835668245867307
32	25.61422771666565	28.087405027686845	25.837213558081373	20.461153697566132
33	24.333922991941332	26.28047416685305	27.36964817036389	22.01595467084172
34	22.027476735595723	27.173773069545824	29.16166812386897	21.637082070989475
35	23.939461716245432	25.75520356777346	27.47876890126946	22.826565814711643
36	23.383013765478537	27.770209362694263	27.116840514290747	21.72993635753645
37	23.314559145469456	28.161959564330395	28.29141335068421	20.23206793951594
38	22.156930521949533	28.470344238628737	26.76643419206604	22.606291047355686
39	23.078695702269847	25.5708505317094	28.94817104166243	22.402282724358322
40	23.655476708484986	25.23942172790305	29.524952047877566	21.580149515734394
41	20.486231132618965	26.586147767091628	29.015270124641628	23.91235097564778
42	22.498525853480004	26.689168581362722	27.4794466697844	23.332858895372873
43	23.986905512291333	25.793158604610184	26.791511627118876	23.428424255979614
44	22.316206122960764	26.65324685007083	27.48893542899358	23.541611597974825
45	23.166127840697285	25.9653118074053	27.018564079624248	23.84999627227317
46	22.78183309272551	27.749876307246023	26.628169415017993	22.84012118501047
47	21.389696563035862	26.282507472397874	29.998034471306674	22.329761493259593
48	22.1318530868967	25.095734802735475	29.576462455013115	23.19594965535471
49	21.776024616552466	24.853771442901394	29.586628982737235	23.78357495780891
50	20.075503412564473	25.86974644679856	29.48631924252591	24.56843089811106
51	20.94169157465959	24.971703164501196	28.12468229600862	25.961922964830592
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63.0
1	191.0
2	319.0
3	535.5
4	752.0
5	529.5
6	307.0
7	291.0
8	275.0
9	273.0
10	271.0
11	280.0
12	289.0
13	280.0
14	271.0
15	292.5
16	314.0
17	347.0
18	380.0
19	404.5
20	429.0
21	562.5
22	696.0
23	748.0
24	800.0
25	946.5
26	1380.0
27	1667.0
28	1911.5
29	2156.0
30	2627.5
31	3099.0
32	3359.5
33	3620.0
34	4046.0
35	4472.0
36	4913.0
37	5354.0
38	5839.0
39	6324.0
40	6891.5
41	7459.0
42	7932.5
43	8406.0
44	9382.0
45	10358.0
46	11767.0
47	13176.0
48	12706.5
49	12237.0
50	11607.5
51	10978.0
52	9806.0
53	8634.0
54	8252.0
55	7870.0
56	7606.5
57	7343.0
58	6895.0
59	6447.0
60	6297.5
61	6148.0
62	5648.0
63	5148.0
64	4252.5
65	3357.0
66	2946.5
67	2536.0
68	2111.0
69	1686.0
70	1350.5
71	1015.0
72	861.5
73	708.0
74	584.0
75	352.0
76	244.0
77	213.5
78	183.0
79	138.5
80	94.0
81	68.5
82	43.0
83	37.5
84	32.0
85	21.5
86	11.0
87	9.0
88	7.0
89	8.5
90	10.0
91	5.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	147543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.85888181750405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.55465235691453	32.592532346502374
2	10.255000940162287	9.61075754186915
3	5.490547753012135	7.718427848152743
4	3.7592027423810697	7.046081481330867
5	2.6469184373056396	6.201581911713873
6	2.0105008895381635	5.652589414611334
7	1.5028132548418358	4.929410409168852
8	1.1846044809580978	4.440739309896098
9	0.9285910583334538	3.916146479331449
>10	2.6339008056467597	15.869949777353042
>50	0.02314245628245368	0.7658784218837899
>100	0.010124824623573486	1.255905058186427
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	450	0.3049958317236331	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	348	0.2358634431996096	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	344	0.23315236913984397	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	296	0.20061948042265645	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	191	0.12945378635380872	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10302081427109384	0.0
2	0.0	0.0	0.0	0.5401815064083013	0.0
3	0.0	0.0	0.0	0.6987793389045905	0.0
4	0.0	0.0	0.0	0.9414204672536142	0.0
5	0.0	0.0	0.0	1.8204862311326189	0.0
6	0.0	0.0	0.0	2.227147340097463	0.0
7	0.0	0.0	0.0	2.652785967480667	0.0
8	0.0	0.0	0.0	3.3664762137139683	0.0
9	0.0	0.0	0.0	3.61318395315264	0.0
10	0.0	0.0	0.0	4.675924984580766	0.0
11	0.0	0.0	0.0	5.6004012389608455	0.0
12	0.0	0.0	0.0	6.587232196715534	0.0
13	0.0	0.0	0.0	6.905105630223054	0.0
14	0.0	0.0	0.0	7.047437018360749	0.0
15	0.0	0.0	0.0	7.321933266912019	0.0
16	0.0	0.0	0.0	7.910914106396102	0.0
17	0.0	0.0	0.0	8.501928251425008	0.0
18	0.0	0.0	0.0	9.13428627586534	0.0
19	0.0	0.0	0.0	9.521292097896884	0.0
20	0.0	0.0	0.0	9.882542716360653	0.0
21	0.0	0.0	0.0	10.384091417417295	0.0
22	0.0	0.0	0.0	10.898517720257823	0.0
23	0.0	0.0	0.0	11.416332865673057	0.0
24	0.0	0.0	0.0	11.796561002555187	0.0
25	0.0	0.0	0.0	12.136123028540831	0.0
26	0.0	0.0	0.0	12.464840758287414	0.0
27	0.0	0.0	0.0	12.811180469422473	0.0
28	0.0	0.0	0.0	13.174464393431068	0.0
29	0.0	0.0	0.0	13.55130368773849	0.0
30	0.0	0.0	0.0	13.957964796703333	0.0
31	0.0	0.0	0.0	14.323959794771694	0.0
32	0.0	0.0	0.0	14.677077191056167	0.0
33	0.0	0.0	0.0	15.01053930040734	0.0
34	0.0	0.0	0.0	15.368401076296403	0.0
35	0.0	0.0	0.0	15.727618389215348	0.0
36	0.0	0.0	0.0	16.123435201941128	0.0
37	0.0	0.0	0.0	16.50976325545773	0.0
38	0.0	0.0	0.0	16.892024697884686	0.0
39	0.0	0.0	0.0	17.287163742095526	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTATT	30	2.1526084E-6	45.000004	8
CCCCATT	30	2.1526084E-6	45.000004	42
ATGGGTA	20	7.0132833E-4	45.0	5
AACCGGG	20	7.0132833E-4	45.0	3
ACTGCGC	20	7.0132833E-4	45.0	27
CGTATGG	20	7.0132833E-4	45.0	2
CTCGTCC	25	3.874235E-5	45.0	37
CCGATTG	20	7.0132833E-4	45.0	11
GATTTAC	20	7.0132833E-4	45.0	9
CAACAAT	45	3.8016879E-10	45.0	26
TACGGCT	35	1.2023156E-7	45.0	7
GTAGCAT	35	1.2023156E-7	45.0	29
ATATCAG	20	7.0132833E-4	45.0	37
TAGGGCG	25	3.874235E-5	45.0	5
AATACGG	20	7.0132833E-4	45.0	2
TACACGC	35	1.2023156E-7	45.0	35
TCAACGC	35	1.2023156E-7	45.0	16
ATTTCCG	20	7.0132833E-4	45.0	32
AATTATC	105	0.0	42.857143	45
GGCGATT	55	6.002665E-11	40.90909	8
>>END_MODULE