##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936321.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	88201
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.836237684379995	31.0	31.0	34.0	30.0	34.0
2	31.93190553395086	33.0	31.0	34.0	30.0	34.0
3	31.85911724356867	33.0	31.0	34.0	30.0	34.0
4	35.630673121619935	37.0	35.0	37.0	33.0	37.0
5	35.70739560775955	37.0	35.0	37.0	35.0	37.0
6	35.65274770127323	37.0	35.0	37.0	33.0	37.0
7	27.064477727009898	35.0	19.0	37.0	0.0	37.0
8	31.12544075463997	35.0	25.0	37.0	17.0	37.0
9	35.7460799764175	38.0	32.0	39.0	32.0	39.0
10	36.57050373578531	37.0	35.0	39.0	32.0	39.0
11	36.906679062595664	39.0	37.0	39.0	33.0	39.0
12	36.91357240847609	39.0	35.0	39.0	33.0	39.0
13	36.755830432761535	39.0	35.0	39.0	33.0	39.0
14	37.99869615990748	40.0	37.0	41.0	33.0	41.0
15	38.01104295869661	40.0	37.0	41.0	33.0	41.0
16	38.00250564052562	40.0	37.0	41.0	33.0	41.0
17	37.82308590605549	40.0	36.0	41.0	33.0	41.0
18	37.745909910318474	39.0	36.0	41.0	33.0	41.0
19	37.568678359655785	39.0	36.0	41.0	32.0	41.0
20	37.72509381979796	39.0	36.0	41.0	33.0	41.0
21	37.72477636307978	39.0	36.0	41.0	33.0	41.0
22	37.77722474801873	39.0	36.0	41.0	33.0	41.0
23	37.80483214476026	39.0	36.0	41.0	33.0	41.0
24	37.697112277638574	39.0	36.0	41.0	33.0	41.0
25	37.63430119839911	39.0	36.0	41.0	33.0	41.0
26	37.438702509041846	39.0	36.0	41.0	33.0	41.0
27	37.25996303896781	39.0	35.0	41.0	32.0	41.0
28	37.254192129340936	39.0	35.0	41.0	32.0	41.0
29	37.104862756658086	39.0	35.0	41.0	32.0	41.0
30	36.818618836521125	39.0	35.0	41.0	31.0	41.0
31	36.724912415959004	39.0	35.0	41.0	30.0	41.0
32	36.394031813698255	39.0	35.0	40.0	30.0	41.0
33	36.37504109930726	39.0	35.0	40.0	30.0	41.0
34	36.19108626886317	39.0	35.0	40.0	30.0	41.0
35	35.796952415505494	38.0	35.0	40.0	27.0	41.0
36	35.681999070305324	38.0	35.0	40.0	27.0	41.0
37	35.04888833459938	38.0	34.0	40.0	24.0	41.0
38	35.16852416639267	38.0	34.0	40.0	24.0	41.0
39	34.75970793981928	38.0	34.0	40.0	23.0	41.0
40	34.66126234396435	38.0	33.0	40.0	23.0	41.0
41	34.6537114091677	38.0	34.0	40.0	23.0	41.0
42	34.427455471026406	38.0	33.0	40.0	22.0	41.0
43	34.51602589539801	38.0	33.0	40.0	23.0	41.0
44	34.06335529075634	38.0	33.0	40.0	20.0	41.0
45	34.054840648065216	37.0	33.0	40.0	21.0	41.0
46	33.5099488667929	37.0	32.0	40.0	17.0	41.0
47	33.34692350426866	37.0	32.0	40.0	15.0	41.0
48	33.287026224192466	37.0	32.0	40.0	15.0	41.0
49	33.11130259294112	37.0	31.0	40.0	14.0	41.0
50	32.97672361991361	37.0	31.0	40.0	14.0	41.0
51	30.374236119771886	35.0	26.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	9.0
11	7.0
12	2.0
13	4.0
14	2.0
15	2.0
16	5.0
17	21.0
18	35.0
19	67.0
20	110.0
21	234.0
22	398.0
23	553.0
24	854.0
25	1161.0
26	1424.0
27	1740.0
28	1945.0
29	2119.0
30	2419.0
31	2741.0
32	3447.0
33	4344.0
34	6377.0
35	7993.0
36	7847.0
37	11629.0
38	14842.0
39	15862.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.941644652554956	19.35238829491729	22.055305495402546	11.650661557125202
2	27.568848425754812	31.235473520708386	23.33533633405517	17.86034171948164
3	28.998537431548392	31.128898765320123	25.181120395460372	14.691443407671114
4	25.716261720388655	24.017868278137435	33.45653677395948	16.80933322751443
5	28.989467239600458	26.573394859468713	23.606308318499792	20.830829582431036
6	22.28092652010748	37.84877722474802	26.460017460119502	13.410278795024999
7	56.39618598428589	26.755932472420945	12.303715377376674	4.5441661659164865
8	72.60688654323647	10.623462319021327	11.047493792587385	5.722157345154817
9	67.11601909275406	7.086087459325858	11.335472386934388	14.462421060985703
10	39.87823267309894	23.872745206970443	20.319497511366084	15.92952460856453
11	29.38288681534223	23.317195950159295	26.537114091676965	20.76280314282151
12	28.458861010646135	20.639221777530867	30.39081189555674	20.511105316266256
13	21.246924638042653	25.18565549143434	34.68894910488543	18.87847076563758
14	18.500924025804697	29.560889332320496	27.793335676466253	24.144850965408555
15	15.308216460130838	25.0031178784821	40.33741114046326	19.3512545209238
16	17.390959286175896	26.643688847065228	30.94749492636138	25.017856940397504
17	18.267366583145318	24.314917064432375	30.928220768472016	26.489495583950294
18	19.100690468362036	26.690173580798405	32.08467024183399	22.124465709005566
19	20.208387660003854	28.272922075713424	27.833017766238477	23.68567249804424
20	21.964603575923174	26.686772258817925	32.03478418612034	19.313839979138557
21	20.009977211142733	30.98604324214011	29.71735014342241	19.286629403294747
22	19.0916202764141	24.303579324497456	28.38403192707566	28.220768472012793
23	18.10637067606943	29.69127334157209	29.47812383079557	22.724232151562905
24	21.890908266346187	23.158467591070394	28.67881316538361	26.271810977199806
25	18.14718653983515	32.218455573066066	27.030305778846042	22.60405210825274
26	19.362592260858722	25.911270847269307	29.071098967131892	25.655037924740082
27	20.779809752723892	28.51554971032074	27.752519812700537	22.952120724254826
28	17.167605809457942	25.39880500221086	32.72752009614403	24.706069092187164
29	23.025816033831816	23.60177322252582	28.899899094114577	24.472511649527785
30	18.826317161936938	27.751386038707043	32.41233092595322	21.0099658734028
31	22.678881191823223	25.433951996009114	25.411276516139274	26.475890296028386
32	23.368215779866443	30.70940238772803	24.605163206766363	21.317218625639164
33	19.68118275303001	27.367036654913207	24.84212197140622	28.109658620650556
34	23.189079488894684	24.489518259430167	28.828471332524575	23.492930919150577
35	18.13131370392626	24.407886531898733	28.198092992142946	29.262706772032065
36	23.073434541558484	23.61084341447376	26.749129828459996	26.56659221550776
37	17.702747134386232	27.724175462863233	32.47695604358227	22.096121359168265
38	17.269645468872234	28.808063400641714	25.270688540946246	28.6516025895398
39	19.905670003741456	27.47814650627544	30.022335347671795	22.593848142311312
40	20.228795591886715	25.564336005260714	30.09489688325529	24.111971519597283
41	17.33086926452081	24.662985680434463	29.14366050271539	28.862484552329338
42	19.126767270212355	21.888640718359202	30.965635310257255	28.018956701171188
43	21.54170587635061	23.835330665185204	26.373850636614097	28.24911282185009
44	20.54511853607102	24.05755036790966	28.33187832337502	27.0654527726443
45	18.9328919173252	24.096098683688393	27.555243137832903	29.415766261153504
46	23.83873198716568	26.616478271221418	26.487228035963312	23.057561705649597
47	17.573496899128127	22.393170145463202	35.41229691273342	24.621036042675254
48	20.49296493237038	23.486128275189625	28.728699221097266	27.29220757134273
49	18.037210462466412	21.740116325211734	33.809140485935536	26.413532726386325
50	19.065543474563782	21.983877733812545	32.34657203433068	26.604006757293003
51	18.715207310574712	21.870500334463326	28.36022267321232	31.05406968174964
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	60.0
1	119.0
2	178.0
3	320.0
4	462.0
5	351.0
6	240.0
7	211.0
8	182.0
9	198.5
10	215.0
11	199.0
12	183.0
13	191.5
14	200.0
15	192.0
16	184.0
17	170.0
18	156.0
19	182.5
20	209.0
21	241.5
22	274.0
23	331.0
24	388.0
25	453.0
26	683.5
27	849.0
28	956.0
29	1063.0
30	1231.5
31	1400.0
32	1691.0
33	1982.0
34	2355.0
35	2728.0
36	2917.5
37	3107.0
38	3221.0
39	3335.0
40	3712.0
41	4089.0
42	4822.5
43	5556.0
44	6325.0
45	7094.0
46	9800.0
47	12506.0
48	10588.0
49	8670.0
50	8289.5
51	7909.0
52	6748.0
53	5587.0
54	4841.0
55	4095.0
56	3658.5
57	3222.0
58	3100.5
59	2979.0
60	2780.5
61	2582.0
62	2289.5
63	1997.0
64	1718.0
65	1439.0
66	1177.5
67	916.0
68	810.5
69	705.0
70	569.5
71	434.0
72	352.0
73	270.0
74	191.0
75	92.5
76	73.0
77	47.5
78	22.0
79	17.5
80	13.0
81	10.0
82	7.0
83	6.0
84	5.0
85	4.0
86	3.0
87	2.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	88201.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.42914479427671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0349106589903	36.594823187945714
2	9.402023074982225	9.294679198648542
3	4.699864669587357	6.969308737996167
4	3.082780925293025	6.09516898901373
5	2.257036034589536	5.578168047981316
6	1.7478266853223845	5.183614698246052
7	1.2409110718627427	4.293602113354724
8	0.9931876046516961	3.9273931134567635
9	0.6674771199853201	2.9693540889559076
>10	1.8212262311626946	12.071291708710785
>50	0.020643622267587217	0.7335517737894128
>100	0.0252310938826066	2.4795637237673045
>500	0.002293735807509691	1.1247038015442001
>1k	0.004587471615019382	2.684776816589381
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG	1258	1.4262876838131087	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC	1110	1.2584891327762724	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	992	1.1247038015442001	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	281	0.3185904921712906	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG	256	0.2902461423339871	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT	225	0.2550991485357309	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTC	217	0.24602895658779378	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	215	0.2437614086008095	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC	212	0.2403600866203331	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTT	195	0.22108592873096677	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	175	0.19841044886112405	No Hit
GAATCTATCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	153	0.17346742100429702	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	144	0.16326345506286777	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	114	0.12925023525810364	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	92	0.10430720740127664	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT	91	0.1031734334077845	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0011337739934921373	0.0	0.0	0.22335347671795105	0.0
2	0.0011337739934921373	0.0	0.0	1.287967256607068	0.0
3	0.0011337739934921373	0.0	0.0	1.575945850954071	0.0
4	0.0011337739934921373	0.0	0.0	2.2085917393226833	0.0
5	0.0011337739934921373	0.0	0.0	5.104250518701602	0.0
6	0.0011337739934921373	0.0	0.0	5.789050010770853	0.0
7	0.0011337739934921373	0.0	0.0	6.773165837122028	0.0
8	0.0011337739934921373	0.0	0.0	8.141631047267039	0.0
9	0.0011337739934921373	0.0	0.0	8.50330495119103	0.0
10	0.0011337739934921373	0.0	0.0	12.050883776827927	0.0
11	0.0011337739934921373	0.0	0.0	13.395539733109603	0.0
12	0.0011337739934921373	0.0	0.0	16.817269645468873	0.0
13	0.0011337739934921373	0.0	0.0	17.35581229237764	0.0
14	0.0011337739934921373	0.0	0.0	17.624516728835275	0.0
15	0.0011337739934921373	0.0	0.0	18.165326923731023	0.0
16	0.0011337739934921373	0.0	0.0	18.96010249316901	0.0
17	0.0011337739934921373	0.0	0.0	19.807031666307637	0.0
18	0.0011337739934921373	0.0	0.0	20.632419133569915	0.0
19	0.0011337739934921373	0.0	0.0	21.172095554472172	0.0
20	0.0011337739934921373	0.0	0.0	21.690230269498077	0.0
21	0.0011337739934921373	0.0	0.0	22.367093343612883	0.0
22	0.0011337739934921373	0.0	0.0	23.055294157662612	0.0
23	0.0011337739934921373	0.0	0.0	23.710615525901066	0.0
24	0.0011337739934921373	0.0	0.0	24.255960816770784	0.0
25	0.0011337739934921373	0.0	0.0	24.743483633972403	0.0
26	0.0011337739934921373	0.0	0.0	25.167515107538463	0.0
27	0.0011337739934921373	0.0	0.0	25.56206845727373	0.0
28	0.0011337739934921373	0.0	0.0	26.01444428067709	0.0
29	0.0011337739934921373	0.0	0.0	26.486094261969818	0.0
30	0.0011337739934921373	0.0	0.0	26.966814435210484	0.0
31	0.0011337739934921373	0.0	0.0	27.480414054262422	0.0
32	0.0011337739934921373	0.0	0.0	27.932789877665787	0.0
33	0.0011337739934921373	0.0	0.0	28.35001870727089	0.0
34	0.0011337739934921373	0.0	0.0	28.74230450901917	0.0
35	0.0011337739934921373	0.0	0.0	29.216222038298884	0.0
36	0.0011337739934921373	0.0	0.0	29.619845579982087	0.0
37	0.0011337739934921373	0.0	0.0	30.06768630741148	0.0
38	0.0011337739934921373	0.0	0.0	30.476978719062142	0.0
39	0.0011337739934921373	0.0	0.0	30.884003582725818	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAA	20	6.99788E-4	45.000004	25
CTCACTA	20	6.99788E-4	45.000004	45
CCTTAAT	20	6.99788E-4	45.000004	23
ATCTATT	20	6.99788E-4	45.000004	15
CTCGTCC	20	6.99788E-4	45.000004	37
TCACCAT	20	6.99788E-4	45.000004	32
CATTGGG	35	1.1949487E-7	45.000004	3
GCTATCT	20	6.99788E-4	45.000004	4
GACCGAT	35	1.1949487E-7	45.000004	9
CATCTAG	20	6.99788E-4	45.000004	39
AGGCGAT	20	6.99788E-4	45.000004	7
ATCGTTG	20	6.99788E-4	45.000004	23
TAGTTGA	20	6.99788E-4	45.000004	11
ACTAGAG	20	6.99788E-4	45.000004	2
CTAGTTG	20	6.99788E-4	45.000004	10
AAGCATG	20	6.99788E-4	45.000004	1
CGGGCTC	20	6.99788E-4	45.000004	6
ACTAATC	20	6.99788E-4	45.000004	10
CGTACAT	20	6.99788E-4	45.000004	35
TCGTACA	20	6.99788E-4	45.000004	34
>>END_MODULE