##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936319.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128656
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.894695933341623	31.0	31.0	34.0	30.0	34.0
2	31.996051486133567	33.0	31.0	34.0	30.0	34.0
3	31.897183186170874	33.0	31.0	34.0	30.0	34.0
4	35.67901224972018	37.0	35.0	37.0	33.0	37.0
5	35.75706535256809	37.0	35.0	37.0	35.0	37.0
6	35.72500310906604	37.0	35.0	37.0	35.0	37.0
7	26.83803320482527	35.0	11.0	37.0	0.0	37.0
8	31.102591406541475	35.0	19.0	37.0	17.0	37.0
9	35.83401473697301	39.0	32.0	39.0	32.0	39.0
10	36.5747963561746	37.0	35.0	39.0	32.0	39.0
11	36.94287091157816	39.0	37.0	39.0	33.0	39.0
12	37.00507555030469	39.0	37.0	39.0	33.0	39.0
13	36.948086369854494	39.0	37.0	39.0	33.0	39.0
14	38.15226650914065	40.0	37.0	41.0	33.0	41.0
15	38.1686823778137	40.0	37.0	41.0	33.0	41.0
16	38.13726526551424	40.0	37.0	41.0	33.0	41.0
17	38.014068523815446	40.0	37.0	41.0	33.0	41.0
18	37.92284075363761	40.0	37.0	41.0	33.0	41.0
19	37.789259731376696	40.0	37.0	41.0	33.0	41.0
20	37.8581799527422	40.0	36.0	41.0	33.0	41.0
21	37.85277017783858	40.0	36.0	41.0	33.0	41.0
22	37.857076234299214	40.0	36.0	41.0	33.0	41.0
23	37.82935113791817	40.0	36.0	41.0	33.0	41.0
24	37.76276271608009	40.0	36.0	41.0	33.0	41.0
25	37.691456286531526	39.0	36.0	41.0	33.0	41.0
26	37.55530251212536	39.0	36.0	41.0	33.0	41.0
27	37.460343862703645	39.0	36.0	41.0	33.0	41.0
28	37.44589447829872	39.0	36.0	41.0	33.0	41.0
29	37.37444814077851	39.0	36.0	41.0	32.0	41.0
30	37.28017348588484	39.0	36.0	41.0	32.0	41.0
31	37.11881295858724	39.0	35.0	41.0	31.0	41.0
32	36.96591686357418	39.0	35.0	41.0	31.0	41.0
33	36.88327788832235	39.0	35.0	41.0	31.0	41.0
34	36.749852319363264	39.0	35.0	41.0	30.0	41.0
35	36.64958493968412	39.0	35.0	41.0	30.0	41.0
36	36.444557579903	39.0	35.0	41.0	30.0	41.0
37	36.34600018654396	39.0	35.0	40.0	30.0	41.0
38	36.21398146996642	39.0	35.0	40.0	30.0	41.0
39	36.15896654644945	39.0	35.0	40.0	29.0	41.0
40	35.94538925506778	39.0	35.0	40.0	28.0	41.0
41	35.94025929610745	38.0	35.0	40.0	29.0	41.0
42	35.81786313891307	38.0	35.0	40.0	28.0	41.0
43	35.82401131700037	38.0	35.0	40.0	28.0	41.0
44	35.569837395846285	38.0	35.0	40.0	27.0	41.0
45	35.54042563114041	38.0	34.0	40.0	27.0	41.0
46	35.26957157070016	38.0	34.0	40.0	26.0	41.0
47	35.1239118268872	38.0	34.0	40.0	25.0	41.0
48	35.013975251834346	38.0	34.0	40.0	24.0	41.0
49	34.81482402686233	38.0	34.0	40.0	24.0	41.0
50	34.649017535132444	38.0	34.0	40.0	24.0	41.0
51	32.21253575425942	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	17.0
11	5.0
12	6.0
13	1.0
14	5.0
15	7.0
16	12.0
17	23.0
18	46.0
19	92.0
20	144.0
21	238.0
22	316.0
23	489.0
24	717.0
25	991.0
26	1385.0
27	1662.0
28	1968.0
29	2261.0
30	2871.0
31	3530.0
32	4571.0
33	6197.0
34	9081.0
35	11424.0
36	12221.0
37	18736.0
38	23975.0
39	25648.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.918666832483524	20.365159805994278	24.056398457903246	11.659774903618953
2	29.764643701032213	28.01423952244746	25.0	17.221116776520333
3	31.005161049620693	26.468256435766698	26.551423952244747	15.975158562367863
4	28.560657878373334	24.797133441114287	28.416863574182315	18.22534510633006
5	26.351666459395595	29.532241014799155	24.567062554408654	19.549029971396592
6	24.082048252704887	37.26215644820296	25.26116154707126	13.394633752020892
7	58.43800522323094	28.395877378435518	8.656417112299465	4.509700286034075
8	77.7701778385773	8.361833105335158	9.06603656261659	4.801952493470962
9	71.94689715209552	8.674294242009701	11.013866434523068	8.36494217137172
10	39.181227459271234	27.92640840691456	18.984734485760477	13.907629648053724
11	30.091095634871284	26.298812336774034	25.752393980848154	17.857698047506528
12	29.515141151598062	22.516633503295612	28.810160427807485	19.15806491729884
13	23.86985449570949	25.806025369978858	31.231345603780625	19.092774530531027
14	20.15296604899888	28.9251958711603	28.454172366621066	22.46766571321975
15	19.145628653152595	28.30493719686606	34.53783733366497	18.011596816316377
16	21.483646312647682	27.383099117025246	29.875792811839325	21.25746175848775
17	21.063922397711725	27.010788459146873	28.70833851511006	23.216950628031338
18	21.859066036562616	28.391991045889814	29.71878497699291	20.03015794055466
19	23.470339510011193	28.430077104837707	27.193446088794925	20.906137296356174
20	24.387513990797164	28.106734237035198	29.760757368486505	17.744994403681133
21	23.78435517970402	29.533018281308294	28.37333664967044	18.30928988931725
22	21.45799651784604	27.014674791692578	28.141711229946527	23.38561746051486
23	21.943788086058948	28.54511254819052	28.41064544210919	21.10045392364134
24	22.057268996393482	25.670780997388388	29.369015047879614	22.902934958338513
25	20.28043775649795	30.33438005223231	27.339572192513366	22.045609998756373
26	19.5109439124487	29.08609003855242	28.744092774530532	22.65887327446835
27	21.064699664220868	29.489491356796414	27.806709364506904	21.639099614475814
28	18.43909339634374	28.953177465489365	30.425320233801767	22.18240890436513
29	20.742911329436637	26.173672428802387	30.15483148862082	22.928584753140157
30	20.731252331799528	28.35312772043278	28.31581892799403	22.59980101977366
31	22.21894043029474	29.285070264892425	25.788925506777765	22.707063798035072
32	22.86173983335406	28.803942295734362	27.784168635741825	20.550149235169755
33	21.85207063798035	28.246642208680512	26.03765078970277	23.863636363636363
34	19.908127098619573	27.743750777266506	28.88633254570327	23.461789578410645
35	20.44832732247233	27.108724039298593	27.454607635866186	24.98834100236289
36	21.23414376321353	29.510477552543218	26.18999502549434	23.06538365874891
37	19.89024996890934	29.41331923890063	29.117957965427188	21.57847282676284
38	20.86338763835344	30.080991170252457	26.155018032583012	22.90060315881109
39	22.620009949011315	25.938160676532767	28.287837333664967	23.153992040790946
40	22.144322845417236	26.308139534883722	28.98970899141898	22.557828628280063
41	18.56345603780624	28.26607387140903	27.121937569953985	26.04853252083074
42	19.001834348961573	27.689342121626666	28.73942917547569	24.569394353936076
43	20.865719437880863	26.469033702275834	27.89687227956722	24.768374580276085
44	20.760788459146873	26.44182937445591	27.898426812585498	24.898955353811715
45	20.18872030841935	26.36643452306927	27.453830369357046	25.991014799154332
46	21.976433279442855	28.772851635368735	26.322907598557393	22.927807486631018
47	19.157287650789705	26.009669195373707	31.63941052107947	23.19363263275712
48	19.826514115159807	26.18766322596692	29.187911951249845	24.79791070762343
49	20.109439124487004	24.807237905733118	30.63595945777888	24.447363512000994
50	19.433217261534637	25.135244372590478	30.010259917920656	25.421278447954233
51	19.05857480412884	24.83521950006218	28.03133938564855	28.07486631016043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	89.0
1	171.5
2	254.0
3	521.0
4	788.0
5	559.0
6	330.0
7	303.0
8	276.0
9	269.5
10	263.0
11	281.0
12	299.0
13	288.5
14	278.0
15	304.0
16	330.0
17	327.5
18	325.0
19	342.5
20	360.0
21	453.5
22	547.0
23	649.0
24	751.0
25	921.5
26	1404.0
27	1716.0
28	2002.5
29	2289.0
30	2535.5
31	2782.0
32	3175.5
33	3569.0
34	3972.5
35	4376.0
36	4638.0
37	4900.0
38	5244.5
39	5589.0
40	6272.5
41	6956.0
42	7410.5
43	7865.0
44	9124.5
45	10384.0
46	12183.0
47	13982.0
48	12801.0
49	11620.0
50	10819.0
51	10018.0
52	8670.0
53	7322.0
54	6671.5
55	6021.0
56	5589.0
57	5157.0
58	4944.5
59	4732.0
60	4540.0
61	4348.0
62	3778.5
63	3209.0
64	2707.0
65	2205.0
66	1787.5
67	1370.0
68	1052.0
69	734.0
70	672.0
71	610.0
72	504.0
73	398.0
74	322.0
75	196.5
76	147.0
77	105.5
78	64.0
79	44.5
80	25.0
81	20.5
82	16.0
83	12.0
84	8.0
85	4.5
86	1.0
87	3.5
88	6.0
89	5.0
90	4.0
91	3.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	128656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.70420345728144
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42643406812398	37.23028852132819
2	9.876751387313364	10.015856236786469
3	4.949872765735659	7.529380674045518
4	3.098077689548395	6.283422459893048
5	2.167581322623172	5.495274219624425
6	1.6939019529693105	5.1532769556025375
7	1.1727013520556764	4.162262156448203
8	0.9121010515988595	3.699788583509514
9	0.7143514118404513	3.259855739335903
>10	1.9361069380997638	13.017659495087674
>50	0.03065885887727259	1.0679641835592588
>100	0.01686237238249992	1.4667019027484143
>500	0.004598828831590888	1.6182688720308418
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	806	0.6264768063673672	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC	712	0.5534137545081457	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	564	0.438378311155329	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	345	0.26815694565352566	No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	219	0.17022136550180325	TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTT	216	0.1678895659743813	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC	185	0.14379430419102102	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	157	0.1220308419350827	No Hit
GCAAGAGGGAACTGAACTAATTATATTTTATATTTTATTTGAAGCAACCTT	136	0.10570824524312897	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	132	0.10259917920656635	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	131	0.1018219126974257	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTC	129	0.1002673796791444	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT	129	0.1002673796791444	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1352443725904738	0.0
2	0.0	0.0	0.0	0.7477303817933093	0.0
3	0.0	0.0	0.0	0.9599241387887079	0.0
4	0.0	0.0	0.0	1.310471334411143	0.0
5	0.0	0.0	0.0	2.793495833851511	0.0
6	0.0	0.0	0.0	3.311155328939187	0.0
7	0.0	0.0	0.0	3.8840007461758486	0.0
8	0.0	0.0	0.0	4.956628528789952	0.0
9	0.0	0.0	0.0	5.279194130083323	0.0
10	0.0	0.0	0.0	7.052916303942296	0.0
11	0.0	0.0	0.0	8.324524312896406	0.0
12	0.0	0.0	0.0	10.06482402686233	0.0
13	0.0	0.0	0.0	10.502425071508519	0.0
14	0.0	0.0	0.0	10.672646437010322	0.0
15	0.0	0.0	0.0	11.022416366123617	0.0
16	0.0	0.0	0.0	11.705633627658251	0.0
17	0.0	0.0	0.0	12.46269120756125	0.0
18	0.0	0.0	0.0	13.268716577540108	0.0
19	0.0	0.0	0.0	13.802698669319737	0.0
20	0.0	0.0	0.0	14.359221489864444	0.0
21	0.0	0.0	0.0	14.972484765576421	0.0
22	0.0	0.0	0.0	15.627720432781992	0.0
23	0.0	0.0	0.0	16.31404676035319	0.0
24	0.0	0.0	0.0	16.854246984205943	0.0
25	0.0	0.0	0.0	17.35014301703768	0.0
26	0.0	0.0	0.0	17.756653401318243	0.0
27	0.0	0.0	0.0	18.196586245491854	0.0
28	0.0	0.0	0.0	18.647400820793433	0.0
29	0.0	0.0	0.0	19.091997264021888	0.0
30	0.0	0.0	0.0	19.62908842183808	0.0
31	0.0	0.0	0.0	20.076793931103097	0.0
32	0.0	0.0	0.0	20.596007959209054	0.0
33	0.0	0.0	0.0	21.03049993781868	0.0
34	0.0	0.0	0.0	21.486755378684244	0.0
35	0.0	0.0	0.0	21.965551548314885	0.0
36	0.0	0.0	0.0	22.430356920780998	0.0
37	0.0	0.0	0.0	22.92081208804875	0.0
38	0.0	0.0	0.0	23.3576358661858	0.0
39	0.0	0.0	0.0	23.808450441487377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTTCG	25	3.8714534E-5	45.000004	23
ACGGGAC	25	3.8714534E-5	45.000004	5
AGGCAGT	25	3.8714534E-5	45.000004	7
GTGTAAT	25	3.8714534E-5	45.000004	8
CGGGAGC	25	3.8714534E-5	45.000004	6
GCTAGCG	25	3.8714534E-5	45.000004	1
CTTAACT	20	7.009924E-4	45.0	45
TCGTTTG	20	7.009924E-4	45.0	1
TTAATCG	20	7.009924E-4	45.0	20
ATAGGAC	20	7.009924E-4	45.0	5
CGTTGAT	20	7.009924E-4	45.0	25
TTGTACT	20	7.009924E-4	45.0	31
AGGCGAT	35	1.2007149E-7	45.0	7
GAGGTCA	35	1.2007149E-7	45.0	33
TATTCTC	20	7.009924E-4	45.0	32
GTCACCA	20	7.009924E-4	45.0	14
ACCAGTT	20	7.009924E-4	45.0	17
GTACTTT	20	7.009924E-4	45.0	33
GCCTCCG	20	7.009924E-4	45.0	25
CGAATAT	35	1.2007149E-7	45.0	14
>>END_MODULE