##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936318.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	183127
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.104572236753725	33.0	31.0	34.0	30.0	34.0
2	32.2276889808712	33.0	31.0	34.0	30.0	34.0
3	32.076274934881255	33.0	31.0	34.0	30.0	34.0
4	35.828228497163174	37.0	35.0	37.0	35.0	37.0
5	35.90812933101072	37.0	35.0	37.0	35.0	37.0
6	35.881492079267396	37.0	35.0	37.0	35.0	37.0
7	26.577413488999436	35.0	10.0	37.0	0.0	37.0
8	31.045361961917138	35.0	19.0	37.0	17.0	37.0
9	35.9224854882131	39.0	32.0	39.0	32.0	39.0
10	36.75537195498206	38.0	35.0	39.0	33.0	39.0
11	37.12750713985376	39.0	37.0	39.0	34.0	39.0
12	37.17907790768155	39.0	37.0	39.0	34.0	39.0
13	37.1810055316802	39.0	37.0	39.0	34.0	39.0
14	38.39835196339153	40.0	38.0	41.0	34.0	41.0
15	38.474921775598354	40.0	38.0	41.0	34.0	41.0
16	38.411577757512546	40.0	38.0	41.0	34.0	41.0
17	38.31111742124318	40.0	37.0	41.0	34.0	41.0
18	38.176107291660976	40.0	37.0	41.0	34.0	41.0
19	38.07788037809826	40.0	37.0	41.0	34.0	41.0
20	38.128047748283976	40.0	37.0	41.0	34.0	41.0
21	38.076700868795974	40.0	37.0	41.0	34.0	41.0
22	38.058205507653156	40.0	37.0	41.0	34.0	41.0
23	38.00761766424394	40.0	37.0	41.0	34.0	41.0
24	37.95902843381915	40.0	36.0	41.0	34.0	41.0
25	37.847903367608275	40.0	36.0	41.0	33.0	41.0
26	37.67823969158016	40.0	36.0	41.0	33.0	41.0
27	37.55303150272761	40.0	36.0	41.0	33.0	41.0
28	37.475123821173284	40.0	36.0	41.0	33.0	41.0
29	37.438269616168014	40.0	36.0	41.0	33.0	41.0
30	37.379234083450285	40.0	36.0	41.0	33.0	41.0
31	37.24532155280215	40.0	35.0	41.0	32.0	41.0
32	37.10498724928601	39.0	35.0	41.0	32.0	41.0
33	37.00265935662136	39.0	35.0	41.0	31.0	41.0
34	36.825716579204595	39.0	35.0	41.0	31.0	41.0
35	36.72224740207616	39.0	35.0	41.0	30.0	41.0
36	36.56547095731378	39.0	35.0	41.0	30.0	41.0
37	36.48920694381495	39.0	35.0	41.0	30.0	41.0
38	36.36484516210062	39.0	35.0	41.0	30.0	41.0
39	36.31164710829097	39.0	35.0	41.0	30.0	41.0
40	36.090816755584925	39.0	35.0	41.0	29.0	41.0
41	35.96560856673238	39.0	35.0	40.0	29.0	41.0
42	35.90748496944743	39.0	35.0	40.0	29.0	41.0
43	35.845746394578626	39.0	35.0	40.0	28.0	41.0
44	35.640511775980606	38.0	35.0	40.0	27.0	41.0
45	35.57383127556286	38.0	35.0	40.0	27.0	41.0
46	35.44669546271167	38.0	34.0	40.0	26.0	41.0
47	35.30426971446045	38.0	34.0	40.0	26.0	41.0
48	35.203842142338374	38.0	34.0	40.0	26.0	41.0
49	35.04192718714335	38.0	34.0	40.0	25.0	41.0
50	34.81843201712473	38.0	34.0	40.0	24.0	41.0
51	32.78796136014897	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	11.0
10	22.0
11	15.0
12	6.0
13	18.0
14	13.0
15	12.0
16	22.0
17	48.0
18	81.0
19	141.0
20	245.0
21	346.0
22	572.0
23	819.0
24	1118.0
25	1414.0
26	1783.0
27	2083.0
28	2520.0
29	2833.0
30	3468.0
31	4449.0
32	5539.0
33	8041.0
34	12563.0
35	15580.0
36	17052.0
37	28008.0
38	35249.0
39	39046.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.536452844201015	20.333429805544785	23.16862068400618	12.961496666248012
2	32.14872738591251	25.58716082281695	25.017064660044667	17.24704713122587
3	33.139296772185425	24.752221136151416	26.188382925510712	15.920099166152452
4	29.524319188322856	25.404773736259536	25.578969785995508	19.491937289422097
5	25.996166594767566	30.112981701223738	24.428948216265216	19.461903487743477
6	26.44612755082538	35.6583136293392	23.941854559950198	13.953704259885216
7	59.86282743669694	29.26165994091532	6.748868271745838	4.126644350641905
8	81.23651891856471	6.7412233040458265	8.255472977769527	3.7667847996199355
9	74.37188399307585	9.213278216756677	10.406985316201325	6.007852473966155
10	42.659465835185415	26.998749501711927	17.200631255904373	13.141153407198283
11	32.28306038978414	27.24884915932659	23.029373058041685	17.438717392847586
12	33.085235929163915	22.03061263494733	26.087906207167705	18.79624522872105
13	27.166938791112177	23.74417753799276	26.948511142540422	22.14037252835464
14	20.24769695348037	27.34714160118388	28.15423176265652	24.250929682679235
15	21.400448868817815	26.866600774326017	31.71897098734758	20.01397936950859
16	23.876872334500103	25.2202023732164	31.323616943432697	19.579308348850795
17	23.747453952721333	25.568594472688353	28.00679309987058	22.67715847471973
18	23.855575638764353	27.03151364899769	27.17786017354077	21.935050538697187
19	27.0260529577834	28.633134382150093	24.63918482801553	19.70162783205098
20	29.12951121352941	25.593713652274104	26.401349882868175	18.875425251328313
21	26.616501116711355	26.982913497190474	26.1528884326178	20.24769695348037
22	24.132432683329057	25.915348364796014	26.733359908697242	23.218859043177687
23	25.564225919716915	27.064277796283452	27.129260021733554	20.24223626226608
24	25.24259120719501	24.895837315087345	27.903586035920426	21.957985441797224
25	24.98211623627319	26.704964314382913	25.430985054088147	22.88193439525575
26	23.21285228284196	28.915452117929085	26.122854630939184	21.748840968289766
27	23.618581640063997	28.485695719364156	26.821277037247377	21.074445603324467
28	22.12453652383319	29.211967650865244	27.494034194848382	21.16946163045318
29	23.37667301927078	27.477652121205505	28.084880984234985	21.060793875288734
30	23.684656003756956	25.78756819038154	27.8566240914775	22.67115171438401
31	24.13953158190764	27.72174501848444	25.49487514129539	22.643848258312538
32	25.752619766610056	28.16187673035653	25.784291775652964	20.30121172738045
33	25.78811425950297	27.7916418660274	25.746066937152907	20.674176937316727
34	24.303898387457885	27.288166136069503	27.53116689510558	20.87676858136703
35	24.12697199211476	25.497059417781102	27.67805948877009	22.697909101334048
36	23.027734850677398	29.403637912486964	26.11794000884632	21.450687227989317
37	25.03672314841613	29.507391045558546	25.888590977845976	19.567294828179353
38	24.509220377115334	27.44324976655545	25.686545402917098	22.360984453412115
39	25.271532870630764	26.034937502389056	26.599026904825614	22.094502722154573
40	25.584430477209807	24.687784979822748	29.122412314950825	20.605372228016623
41	21.618330448268143	27.161478099897884	29.033403048157837	22.18678840367614
42	23.387048332577937	27.733212470034456	26.42428478596821	22.4554544114194
43	24.41584255735091	26.517662605732635	26.611040425497055	22.4554544114194
44	23.28711768335636	27.63273575169145	26.625238222654225	22.45490834229797
45	23.660082893292632	26.319439514653766	26.256095496567955	23.764382095485647
46	22.942001998612984	28.326243535906777	25.551120260802612	23.180634204677627
47	23.622404123914002	26.233160593467918	27.976213229070535	22.168222053547538
48	22.9441862750987	24.794814527622908	29.29169374259394	22.969305454684456
49	22.822958930141375	26.306879924860887	27.447618319526885	23.42254282547085
50	20.172339414723115	26.64926526399712	28.846101339507555	24.332293981772214
51	20.88495961818847	25.960672101874653	27.70208653011298	25.45228174982389
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	89.0
1	263.5
2	438.0
3	983.5
4	1529.0
5	1046.0
6	563.0
7	511.0
8	459.0
9	463.0
10	467.0
11	462.0
12	457.0
13	444.5
14	432.0
15	468.5
16	505.0
17	540.0
18	575.0
19	566.5
20	558.0
21	676.5
22	795.0
23	813.5
24	832.0
25	1129.0
26	1579.5
27	1733.0
28	2120.5
29	2508.0
30	3056.5
31	3605.0
32	4193.5
33	4782.0
34	5087.0
35	5392.0
36	5629.0
37	5866.0
38	6371.5
39	6877.0
40	7675.0
41	8473.0
42	9335.0
43	10197.0
44	10908.5
45	11620.0
46	12334.5
47	13049.0
48	12924.5
49	12800.0
50	12559.5
51	12319.0
52	11596.0
53	10873.0
54	10428.5
55	9984.0
56	9973.5
57	9963.0
58	10175.0
59	10387.0
60	10323.0
61	10259.0
62	9162.0
63	8065.0
64	6782.0
65	5499.0
66	4441.0
67	3383.0
68	2823.5
69	2264.0
70	1924.5
71	1585.0
72	1253.5
73	922.0
74	754.0
75	519.5
76	453.0
77	350.0
78	247.0
79	185.5
80	124.0
81	103.0
82	82.0
83	62.5
84	43.0
85	33.0
86	23.0
87	18.5
88	14.0
89	12.5
90	11.0
91	8.0
92	5.0
93	5.0
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	183127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.08838128730335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.42081168477274	29.948068826552067
2	10.835785827610847	9.771360858857514
3	6.502440383194662	8.795535338863193
4	4.215867940752583	7.603466446782833
5	3.007181872131187	6.779448142545884
6	2.2502391938863253	6.087578565694845
7	1.8239290774988193	5.756660678108635
8	1.3455413048480664	4.853462351264423
9	0.9906865772868753	4.0201608719631725
>10	2.576027322602914	14.712194269550642
>50	0.02058884084826024	0.6252491440366522
>100	0.009688866281534233	0.6689346737510034
>500	0.001211108285191779	0.3778798320291382
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	692	0.3778798320291382	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	201	0.10975989340730749	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGC	187	0.10211492570729602	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05296870477865088	0.0
2	0.0	0.0	0.0	0.26757386950040135	0.0
3	0.0	0.0	0.0	0.3522145833219569	0.0
4	0.0	0.0	0.0	0.4761722738864285	0.0
5	0.0	0.0	0.0	0.9299557137942521	0.0
6	0.0	0.0	0.0	1.1931610303232183	0.0
7	0.0	0.0	0.0	1.3952066052520928	0.0
8	0.0	0.0	0.0	1.7681718151883665	0.0
9	0.0	0.0	0.0	1.9374532428314777	0.0
10	0.0	0.0	0.0	2.4278233138750704	0.0
11	0.0	0.0	0.0	2.9624249837544436	0.0
12	0.0	0.0	0.0	3.5499953584124677	0.0
13	0.0	0.0	0.0	3.7842590115056765	0.0
14	0.0	0.0	0.0	3.8945649740344135	0.0
15	0.0	0.0	0.0	4.0780441988346885	0.0
16	0.0	0.0	0.0	4.412238501149475	0.0
17	0.0	0.0	0.0	4.768821637442867	0.0
18	0.0	0.0	0.0	5.133049741436271	0.0
19	0.0	0.0	0.0	5.34055600757944	0.0
20	0.0	0.0	0.0	5.59120173431553	0.0
21	0.0	0.0	0.0	5.890447612858836	0.0
22	0.0	0.0	0.0	6.199522735587871	0.0
23	0.0	0.0	0.0	6.476925849274001	0.0
24	0.0	0.0	0.0	6.721564815674368	0.0
25	0.0	0.0	0.0	6.953644192281859	0.0
26	0.0	0.0	0.0	7.17698646294648	0.0
27	0.0	0.0	0.0	7.395414111518236	0.0
28	0.0	0.0	0.0	7.622032796911433	0.0
29	0.0	0.0	0.0	7.85848072649036	0.0
30	0.0	0.0	0.0	8.115679282683603	0.0
31	5.460691214293906E-4	0.0	0.0	8.366325009419691	0.0
32	5.460691214293906E-4	0.0	0.0	8.620793220005789	0.0
33	5.460691214293906E-4	0.0	0.0	8.858333287827573	0.0
34	5.460691214293906E-4	0.0	0.0	9.07512272903504	0.0
35	5.460691214293906E-4	0.0	0.0	9.327952732256849	0.0
36	5.460691214293906E-4	0.0	0.0	9.603717638578692	0.0
37	5.460691214293906E-4	0.0	0.0	9.871837577200521	0.0
38	5.460691214293906E-4	0.0	0.0	10.149786760008082	0.0
39	5.460691214293906E-4	0.0	0.0	10.427189873694212	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGAA	20	7.017741E-4	45.000004	11
ACCGCTT	20	7.017741E-4	45.000004	45
TTTCGCA	20	7.017741E-4	45.000004	13
TGGGTAC	20	7.017741E-4	45.000004	6
CGCATCA	20	7.017741E-4	45.000004	26
TCTAGAC	20	7.017741E-4	45.000004	28
AGAACGC	20	7.017741E-4	45.000004	34
CTAGTCC	20	7.017741E-4	45.000004	38
TCCACCG	20	7.017741E-4	45.000004	42
AACGATG	20	7.017741E-4	45.000004	1
GTACGGG	20	7.017741E-4	45.000004	3
ACGATGG	25	3.8779268E-5	45.0	2
AAGGTAT	40	3.437035E-7	39.375004	6
AGGTATG	35	6.219563E-6	38.571426	7
TACGGCT	35	6.219563E-6	38.571426	7
TACGAAA	70	0.0	38.571426	20
CATACGA	70	0.0	38.571426	18
ATACGAA	70	0.0	38.571426	19
ACATACG	70	0.0	38.571426	17
TAGTAAG	35	6.219563E-6	38.571426	2
>>END_MODULE