##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936316.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	131259
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01711882613763	33.0	31.0	34.0	30.0	34.0
2	32.137834358024975	33.0	31.0	34.0	30.0	34.0
3	31.981540313426127	33.0	31.0	34.0	30.0	34.0
4	35.76736833283813	37.0	35.0	37.0	35.0	37.0
5	35.857053611561874	37.0	35.0	37.0	35.0	37.0
6	35.838418698908264	37.0	35.0	37.0	35.0	37.0
7	26.9283477704386	36.0	11.0	37.0	0.0	37.0
8	31.21733366854844	36.0	19.0	37.0	17.0	37.0
9	35.98196695083766	39.0	32.0	39.0	32.0	39.0
10	36.71211878804501	38.0	35.0	39.0	33.0	39.0
11	37.08012402959035	39.0	37.0	39.0	34.0	39.0
12	37.1136760145971	39.0	37.0	39.0	34.0	39.0
13	37.047829101242584	39.0	37.0	39.0	33.0	39.0
14	38.23219741122514	40.0	37.0	41.0	34.0	41.0
15	38.31752489353111	40.0	37.0	41.0	34.0	41.0
16	38.28380530096984	40.0	37.0	41.0	34.0	41.0
17	38.14677088809148	40.0	37.0	41.0	33.0	41.0
18	38.045284513823816	40.0	37.0	41.0	33.0	41.0
19	37.934358786826046	40.0	37.0	41.0	33.0	41.0
20	37.97377703624132	40.0	36.0	41.0	34.0	41.0
21	37.93356646020463	40.0	36.0	41.0	33.0	41.0
22	37.942838205380205	40.0	36.0	41.0	33.0	41.0
23	37.88013774293572	40.0	36.0	41.0	33.0	41.0
24	37.82556624688593	40.0	36.0	41.0	33.0	41.0
25	37.76038214522433	40.0	36.0	41.0	33.0	41.0
26	37.610297198668285	40.0	36.0	41.0	33.0	41.0
27	37.47726251152302	39.0	35.0	41.0	33.0	41.0
28	37.44097547596736	39.0	36.0	41.0	33.0	41.0
29	37.43775283980527	39.0	36.0	41.0	33.0	41.0
30	37.333272385131686	39.0	36.0	41.0	32.0	41.0
31	37.22867003405481	39.0	35.0	41.0	32.0	41.0
32	37.09536869852734	39.0	35.0	41.0	32.0	41.0
33	36.96038366892937	39.0	35.0	41.0	31.0	41.0
34	36.78109691525914	39.0	35.0	41.0	31.0	41.0
35	36.684600675001334	39.0	35.0	41.0	30.0	41.0
36	36.449454894521516	39.0	35.0	41.0	30.0	41.0
37	36.37045840666164	39.0	35.0	41.0	30.0	41.0
38	36.21558902627629	39.0	35.0	41.0	30.0	41.0
39	36.12901210583655	39.0	35.0	40.0	29.0	41.0
40	35.866340593787854	39.0	35.0	40.0	28.0	41.0
41	35.760321197022684	38.0	35.0	40.0	28.0	41.0
42	35.68549966097563	38.0	35.0	40.0	27.0	41.0
43	35.618502350315026	38.0	35.0	40.0	27.0	41.0
44	35.39962212114979	38.0	34.0	40.0	26.0	41.0
45	35.2942350619767	38.0	34.0	40.0	26.0	41.0
46	35.148363159859514	38.0	34.0	40.0	25.0	41.0
47	34.970211566444966	38.0	34.0	40.0	24.0	41.0
48	34.85769356767917	38.0	34.0	40.0	24.0	41.0
49	34.70549067111588	38.0	34.0	40.0	24.0	41.0
50	34.471457195316134	37.0	34.0	40.0	23.0	41.0
51	32.313380415819104	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	14.0
11	10.0
12	7.0
13	1.0
14	3.0
15	11.0
16	11.0
17	35.0
18	48.0
19	79.0
20	167.0
21	275.0
22	369.0
23	554.0
24	816.0
25	1161.0
26	1400.0
27	1721.0
28	1951.0
29	2230.0
30	2571.0
31	3243.0
32	4292.0
33	6041.0
34	9350.0
35	11650.0
36	12484.0
37	19346.0
38	24755.0
39	26653.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.886514448533056	19.920157855842266	24.029590351899678	12.163737343725002
2	32.5760519278678	25.357499295286416	24.98876267532131	17.077686101524467
3	32.31702207086752	24.98876267532131	26.52084809422592	16.173367159585247
4	28.880305350490254	25.20208138108625	26.875871368820427	19.041741899603075
5	26.002788380225354	29.648252691244032	25.21198546385391	19.136973464676707
6	26.232105988922665	34.90655878834975	25.115230193739098	13.74610502898849
7	60.57489391203651	27.859422972900905	7.242931913240234	4.322751201822352
8	81.24776205822077	6.3576592843157425	8.359045855903215	4.035532801560274
9	75.08208960909347	8.579221234353454	10.069404764625665	6.269284391927411
10	41.14460722693301	28.20758957481011	17.120349842677456	13.527453355579427
11	32.940979285229965	26.666361925658432	23.325638622875385	17.06702016623622
12	31.88581354421411	23.127556967522228	26.525419209349455	18.46121027891421
13	25.338452982271694	25.62719508757495	28.734029666537154	20.30032226361621
14	20.493832803845834	29.397603211970225	27.685720598206597	22.42284338597734
15	20.26832445775147	26.929962897782247	33.16039281115962	19.64131983330667
16	23.423917598031373	25.672906238810288	31.021872785866112	19.881303377292223
17	23.0528954205045	25.484728666224793	28.09407354924234	23.36830236402837
18	23.421632040469607	27.192040164864885	28.142070258039446	21.24425753662606
19	25.921270160522326	27.969891588386318	25.841275645860474	20.26756260523088
20	27.01757593764999	26.920820667535178	27.00081518219703	19.0607882126178
21	24.902673340494747	27.805331443939078	27.2118483304002	20.08014688516597
22	24.027304794337912	25.570056148530767	27.070905614091224	23.331733443040097
23	23.686756717634598	27.592774590694734	27.436594823973977	21.28387386769669
24	22.627019861495214	26.018787283157724	29.684821612232305	21.669371243114757
25	22.662826929962897	28.079598351351144	26.881966188985135	22.375608529700823
26	21.909354787100312	28.725649288810672	27.218705003085503	22.146290921003512
27	22.892144538660204	28.86659200511965	27.02595631537647	21.215307140843677
28	20.346033414851554	29.623873410585176	29.090576646172835	20.939516528390435
29	22.251426568844803	27.766476965389042	27.998841984168703	21.983254481597452
30	23.41706092534607	26.862158023449823	28.32796227306318	21.392818778140928
31	24.22691015473225	27.412977395835714	26.390571313205193	21.96954113622685
32	24.57279119907968	28.68679481026063	26.059165466748947	20.681248523910742
33	24.43718145041483	27.406120723150412	25.106849816012616	23.049848010422142
34	22.280376964627187	27.413739248356308	28.532138748581048	21.77374503843546
35	23.50695952277558	25.87098789416345	27.092999337188306	23.529053245872664
36	23.324114917834205	29.352653913255473	26.553607752611246	20.769623416299073
37	23.05899024066921	29.073054038199288	26.74864199788205	21.11931372324945
38	22.59578390815106	28.379006391942646	25.243221417198058	23.781988282708234
39	23.494769882446157	26.076688074722494	27.115854912805982	23.31268713002537
40	24.879817764877075	25.32473963690109	28.617466230887022	21.17797636733481
41	21.17111969464951	26.93605771794696	28.186257704233615	23.706564883169918
42	23.007184269269153	26.532275882034757	27.348981784106236	23.111558064589858
43	23.17402997127816	26.551322195049483	26.550560342528897	23.724087491143464
44	21.883451801400287	27.80609329645967	26.635125972314277	23.675328929825763
45	22.515789393489207	26.354764244737506	26.376096115313995	24.75335024645929
46	23.430012418196085	27.809140706542028	25.80242116731043	22.958425707951456
47	22.714632901362954	26.606175576531893	28.666224792204726	22.012966729900427
48	22.444937109074424	26.20087003557851	27.93179896235687	23.422393892990197
49	22.341325166274313	26.158206294425522	28.279965564266067	23.220502975034094
50	20.400124943813378	26.442377284605247	28.471190546933922	24.686307224647454
51	21.090363327467067	25.435208252386506	27.02595631537647	26.44847210476996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	48.0
1	177.5
2	307.0
3	640.5
4	974.0
5	700.0
6	426.0
7	391.5
8	357.0
9	351.0
10	345.0
11	349.5
12	354.0
13	362.0
14	370.0
15	402.5
16	435.0
17	424.5
18	414.0
19	421.5
20	429.0
21	535.0
22	641.0
23	636.0
24	631.0
25	790.0
26	1183.5
27	1418.0
28	1722.5
29	2027.0
30	2330.5
31	2634.0
32	2920.0
33	3206.0
34	3525.0
35	3844.0
36	4175.0
37	4506.0
38	4816.0
39	5126.0
40	5757.5
41	6389.0
42	6943.5
43	7498.0
44	8088.0
45	8678.0
46	8810.5
47	8943.0
48	9855.5
49	10768.0
50	10266.0
51	9764.0
52	9273.5
53	8783.0
54	8091.0
55	7399.0
56	7001.5
57	6604.0
58	6493.0
59	6382.0
60	6145.0
61	5908.0
62	5315.0
63	4722.0
64	4064.0
65	3406.0
66	2824.0
67	2242.0
68	1848.5
69	1455.0
70	1236.0
71	1017.0
72	878.0
73	739.0
74	630.0
75	392.0
76	263.0
77	205.5
78	148.0
79	123.5
80	99.0
81	79.0
82	59.0
83	36.0
84	13.0
85	11.5
86	10.0
87	5.5
88	1.0
89	2.0
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	131259.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.94261726814923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.47261199129177	34.26584081853435
2	9.294601852881819	8.912150785850875
3	5.16296143272577	7.42577651818161
4	3.586581703189309	6.878004555878073
5	2.5536715981502964	6.121485002933133
6	1.822689062276534	5.243069046693941
7	1.4969251060719222	5.02365552076429
8	1.1663938724594385	4.473598000898986
9	0.8708226731713519	3.7574566315452653
>10	2.5361915809880977	15.883863201761402
>50	0.025425479508652607	0.7991832940979284
>100	0.011123647285035516	1.2159166228601466
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	462	0.35197586451214774	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC	266	0.2026527704766911	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG	234	0.17827348981784105	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	212	0.16151273436488164	TruSeq Adapter, Index 22 (95% over 21bp)
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	153	0.11656343565012685	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	140	0.10665935288246903	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06856672685301579	0.0
2	0.0	0.0	0.0	0.37711699769158685	0.0
3	0.0	0.0	0.0	0.5066319261917278	0.0
4	0.0	0.0	0.0	0.7412825025331596	0.0
5	0.0	0.0	0.0	1.4726609222986615	0.0
6	0.0	0.0	0.0	1.8398738372225905	0.0
7	0.0	0.0	0.0	2.1948971118170943	0.0
8	0.0	0.0	0.0	2.8927540206766773	0.0
9	0.0	0.0	0.0	3.1304520071004656	0.0
10	0.0	0.0	0.0	4.0538172620544115	0.0
11	0.0	0.0	0.0	4.914710610320054	0.0
12	0.0	0.0	0.0	5.9020714770034814	0.0
13	0.0	0.0	0.0	6.196146549950861	0.0
14	0.0	0.0	0.0	6.322614068368646	0.0
15	0.0	0.0	0.0	6.560312054792433	0.0
16	0.0	0.0	0.0	7.018185419666461	0.0
17	0.0	0.0	0.0	7.5522440365993955	0.0
18	0.0	0.0	0.0	8.11906231191766	0.0
19	0.0	0.0	0.0	8.467228913826862	0.0
20	0.0	0.0	0.0	8.822252188421366	0.0
21	0.0	0.0	0.0	9.3075522440366	0.0
22	0.0	0.0	0.0	9.776853396719464	0.0
23	0.0	0.0	0.0	10.24310713931997	0.0
24	0.0	0.0	0.0	10.61870043197038	0.0
25	0.0	0.0	0.0	10.976771116647239	0.0
26	0.0	0.0	0.0	11.278464714800508	0.0
27	0.0	0.0	0.0	11.604537593612628	0.0
28	0.0	0.0	0.0	11.954989753083598	0.0
29	0.0	0.0	0.0	12.323726373048705	0.0
30	0.0	0.0	0.0	12.764077129949184	0.0
31	0.0	0.0	0.0	13.147288947805484	0.0
32	0.0	0.0	0.0	13.529738913141195	0.0
33	0.0	0.0	0.0	13.868001432282739	0.0
34	0.0	0.0	0.0	14.229881379562544	0.0
35	0.0	0.0	0.0	14.600141704568829	0.0
36	0.0	0.0	0.0	14.978020554781006	0.0
37	0.0	0.0	0.0	15.364279782719661	0.0
38	0.0	0.0	0.0	15.708637122025918	0.0
39	0.0	0.0	0.0	16.12232304070578	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGTC	20	7.0104457E-4	45.0	12
AGGTACC	20	7.0104457E-4	45.0	7
CAACGGG	20	7.0104457E-4	45.0	3
CGTGCGG	20	7.0104457E-4	45.0	2
GGCCGAT	20	7.0104457E-4	45.0	8
CCTCGAT	20	7.0104457E-4	45.0	15
TAGCCGG	20	7.0104457E-4	45.0	2
GTCAAGC	20	7.0104457E-4	45.0	16
TAGGGCG	25	3.8718856E-5	45.0	5
ATGGTCA	25	3.8718856E-5	45.0	13
TAGGCGA	25	3.8718856E-5	45.0	6
GGCGATT	20	7.0104457E-4	45.0	8
CTGGGAC	25	3.8718856E-5	45.0	5
CTAGGAC	20	7.0104457E-4	45.0	5
TCACGTC	25	3.8718856E-5	45.0	39
TCCGGGT	20	7.0104457E-4	45.0	17
CGTTTTA	55	6.002665E-11	40.909092	1
AGGCGAT	50	1.0659278E-9	40.5	7
ATCTATC	45	1.9059371E-8	40.000004	3
GGCGATG	90	0.0	40.000004	8
>>END_MODULE