##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936315.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85588
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.896141982520913	31.0	31.0	34.0	30.0	34.0
2	31.999976632238162	33.0	31.0	34.0	30.0	34.0
3	31.916401832032527	33.0	31.0	34.0	30.0	34.0
4	35.667698742814416	37.0	35.0	37.0	33.0	37.0
5	35.76379866336402	37.0	35.0	37.0	35.0	37.0
6	35.72695938682993	37.0	35.0	37.0	35.0	37.0
7	27.004147777725848	35.0	18.0	37.0	0.0	37.0
8	31.182291910080853	35.0	25.0	37.0	17.0	37.0
9	35.81602561106697	38.0	32.0	39.0	32.0	39.0
10	36.658199747628174	37.0	35.0	39.0	33.0	39.0
11	37.014826844884794	39.0	37.0	39.0	34.0	39.0
12	37.05441183343459	39.0	37.0	39.0	33.0	39.0
13	36.949233537411786	39.0	37.0	39.0	33.0	39.0
14	38.13235500303781	40.0	37.0	41.0	33.0	41.0
15	38.187234191709116	40.0	37.0	41.0	33.0	41.0
16	38.15139972893396	40.0	37.0	41.0	33.0	41.0
17	38.021591811936254	40.0	37.0	41.0	33.0	41.0
18	37.93869467682386	40.0	37.0	41.0	33.0	41.0
19	37.82839884095901	40.0	37.0	41.0	33.0	41.0
20	37.90944992288639	40.0	36.0	41.0	33.0	41.0
21	37.86848623638828	40.0	36.0	41.0	33.0	41.0
22	37.8615109594803	40.0	36.0	41.0	33.0	41.0
23	37.86700238351171	40.0	36.0	41.0	33.0	41.0
24	37.80993830910875	40.0	36.0	41.0	33.0	41.0
25	37.755211010889376	40.0	36.0	41.0	33.0	41.0
26	37.567007057064075	39.0	36.0	41.0	33.0	41.0
27	37.436661681544145	39.0	36.0	41.0	33.0	41.0
28	37.43400944057578	39.0	36.0	41.0	33.0	41.0
29	37.41495303079871	39.0	36.0	41.0	32.0	41.0
30	37.29062485395149	39.0	36.0	41.0	32.0	41.0
31	37.1868953591625	39.0	35.0	41.0	32.0	41.0
32	37.00038556807029	39.0	35.0	41.0	31.0	41.0
33	36.902170865074545	39.0	35.0	41.0	31.0	41.0
34	36.782364350142544	39.0	35.0	41.0	31.0	41.0
35	36.550042061971304	39.0	35.0	41.0	30.0	41.0
36	36.406762630275274	39.0	35.0	41.0	30.0	41.0
37	36.22424872645698	39.0	35.0	40.0	30.0	41.0
38	36.192550357526756	39.0	35.0	40.0	30.0	41.0
39	36.19274898350236	39.0	35.0	40.0	30.0	41.0
40	35.91203206056924	39.0	35.0	40.0	28.0	41.0
41	35.7429429359256	38.0	35.0	40.0	27.0	41.0
42	35.63024022059167	38.0	35.0	40.0	27.0	41.0
43	35.60292330700565	38.0	35.0	40.0	27.0	41.0
44	35.42052624199654	38.0	34.0	40.0	26.0	41.0
45	35.36092676543441	38.0	34.0	40.0	26.0	41.0
46	35.12074122540543	38.0	34.0	40.0	25.0	41.0
47	34.85368275926532	38.0	34.0	40.0	23.0	41.0
48	34.725884469785484	38.0	34.0	40.0	23.0	41.0
49	34.506075618077304	38.0	33.0	40.0	23.0	41.0
50	34.28433892601767	37.0	33.0	40.0	22.0	41.0
51	31.87339346637379	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	5.0
11	8.0
12	4.0
13	2.0
14	2.0
15	3.0
16	10.0
17	16.0
18	28.0
19	54.0
20	121.0
21	180.0
22	232.0
23	363.0
24	548.0
25	743.0
26	993.0
27	1159.0
28	1332.0
29	1497.0
30	1912.0
31	2338.0
32	2916.0
33	4029.0
34	6023.0
35	7511.0
36	8010.0
37	12560.0
38	15755.0
39	17216.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86259756040567	20.44562321820816	23.34906762630275	11.342711595083422
2	30.39327943169603	28.67458054867505	23.926251343646303	17.005888675982614
3	32.18324998831612	27.198906388746085	24.523297658550263	16.094545964387528
4	29.963312613917836	24.93690704304342	27.773753329906057	17.326027013132684
5	27.37533299060616	29.42001215123615	22.603636023741647	20.601018834416042
6	24.67518811048278	37.123194840398185	24.483572463429454	13.718044585689581
7	57.515072206384076	28.148805907370196	9.398513810347245	4.93760807589849
8	74.98364256671496	9.039818666168156	10.689582651773613	5.286956115343273
9	66.54320699163434	10.88002991073515	12.613917838949384	9.962845258681122
10	39.28938636257419	26.95938682992943	19.429125578352107	14.322101229144272
11	31.044071598822264	26.968733934663735	23.942608776931344	18.04458568958265
12	30.290461279618636	22.67490769734075	29.077674440342104	17.956956582698506
13	24.55133897275319	26.650932373697245	29.83011637145394	18.967612282095622
14	18.90101416086367	31.858438098798896	27.233958031499743	22.006589708837687
15	17.745478338084776	27.61134738514745	36.86381268402113	17.77936159274665
16	20.24933401878768	27.128803103238774	32.04888535776043	20.572977520213115
17	20.134831985792403	26.425433471982053	29.306678506332663	24.13305603589288
18	20.353320558956863	28.786745805486753	29.22722811609104	21.632705519465347
19	24.516287329999535	28.446744870776275	26.563303266813104	20.473664532411085
20	26.585502640557085	27.190727672103566	28.498153946814973	17.725615740524372
21	23.781371220264525	28.99121372154975	26.906809365798946	20.320605692386785
22	21.02514371173529	27.330934243118193	26.38570827686124	25.258213768285277
23	22.059167172968174	28.520353320558954	28.11141748843296	21.309062018039914
24	22.933121465626023	24.939243819226995	28.69561153432724	23.43202318081974
25	20.543767817918397	29.566060662709724	27.299387764639903	22.590783754731973
26	21.099920549609756	29.966817778193207	26.854231901668456	22.079029770528578
27	22.777725849418143	30.931906342010564	26.456979950460347	19.833387858110953
28	18.750292097022946	29.088189933168202	30.515960181333835	21.64555778847502
29	23.028929289152686	28.106743936065804	27.834509510679066	21.029817264102444
30	20.77978221245969	30.77651072580268	26.41725475533953	22.02645230639809
31	23.512641959153154	28.720147684254805	26.035191849324672	21.732018507267373
32	24.467215030144413	31.343178950320137	25.96275178763378	18.22685423190167
33	22.776557461326355	31.05458709164836	24.88549796700472	21.283357480020566
34	24.369070430434174	27.84619339159695	27.50736084497827	20.277375332990605
35	22.76954713277562	27.739870075244195	27.03065850352853	22.459924288451653
36	25.55848950787494	28.710800579520495	26.606533626209284	19.12417628639529
37	21.80913212132542	31.123521989063885	29.46791606299949	17.599429826611207
38	21.18287610412675	30.128055334860026	26.94069262046081	21.748375940552414
39	25.123849137729586	27.183717343552832	27.128803103238774	20.563630415478805
40	23.85731644623078	26.69182595690985	30.264756741599292	19.186100855260083
41	21.94933869234005	27.011964294059915	27.949011543674345	23.08968546992569
42	21.894424452025984	26.64275365705473	30.28345095106791	21.17937093985138
43	25.07244006169089	26.191755853624336	27.078562415291863	21.657241669392903
44	21.991400663644438	28.318222180679538	27.71650231340842	21.97387484226761
45	21.30088330139739	26.70818339019489	27.024816563069585	24.966116745338134
46	22.92727952516708	29.181660980511285	26.025844744590366	21.865214749731273
47	21.08356311632472	26.26419591531523	31.581530121045002	21.070710847315045
48	22.152638220311257	25.585362433986074	29.596438753096226	22.66556059260644
49	20.28321727344955	25.073608449782682	31.579193344861427	23.063980931906343
50	19.79132588680656	26.552787773987006	29.020423423844466	24.635462915361966
51	20.491190353787914	25.87161751647427	26.9523765013787	26.684815628359114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45.0
1	121.0
2	197.0
3	347.5
4	498.0
5	369.5
6	241.0
7	210.5
8	180.0
9	186.5
10	193.0
11	193.5
12	194.0
13	208.5
14	223.0
15	236.5
16	250.0
17	256.5
18	263.0
19	242.5
20	222.0
21	307.0
22	392.0
23	439.0
24	486.0
25	616.0
26	903.5
27	1061.0
28	1223.0
29	1385.0
30	1604.5
31	1824.0
32	2054.0
33	2284.0
34	2475.0
35	2666.0
36	2793.0
37	2920.0
38	3388.0
39	3856.0
40	4334.0
41	4812.0
42	5582.0
43	6352.0
44	8074.5
45	9797.0
46	8885.0
47	7973.0
48	7588.5
49	7204.0
50	6244.5
51	5285.0
52	4760.5
53	4236.0
54	3830.0
55	3424.0
56	3321.5
57	3219.0
58	3236.5
59	3254.0
60	3202.5
61	3151.0
62	2836.0
63	2521.0
64	2080.0
65	1639.0
66	1269.5
67	900.0
68	777.0
69	654.0
70	540.0
71	426.0
72	343.5
73	261.0
74	211.5
75	130.0
76	98.0
77	72.5
78	47.0
79	34.5
80	22.0
81	14.0
82	6.0
83	6.5
84	7.0
85	6.0
86	5.0
87	4.5
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	85588.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.936159274664675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.48312622773523	38.899144739916814
2	10.311872337607875	10.917418329672383
3	5.228772595846117	8.303734168341355
4	3.2776392168980513	6.940225265224097
5	2.2292361003818395	5.9003598635322705
6	1.609022888295407	5.110529513483199
7	1.1499326814840973	4.261111370752909
8	0.8254795064780277	3.4958171706313967
9	0.5606197717791953	2.670935177828668
>10	1.2779482199218664	9.294527270178063
>50	0.024278809014059637	0.9826143851941862
>100	0.017657315646588827	1.8425480207505724
>500	0.004414328911647207	1.381034724494088
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	616	0.7197270645417582	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	566	0.6613076599523298	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	498	0.5818572697107071	No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	244	0.2850866943964107	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	220	0.2570453801934851	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	153	0.17876337804365097	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	127	0.1483852876571482	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	122	0.14254334719820536	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	108	0.12618591391316542	TruSeq Adapter, Index 15 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTT	105	0.1226807496377997	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	100	0.11683880917885685	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC	92	0.1074917044445483	No Hit
GAATCTATCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	88	0.10281815207739402	No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	87	0.10164976398560545	TruSeq Adapter, Index 21 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16824788521755385	0.0
2	0.0	0.0	0.0	1.0468757302425573	0.0
3	0.0	0.0	0.0	1.3658456793008367	0.0
4	0.0	0.0	0.0	1.8074963779969155	0.0
5	0.0	0.0	0.0	3.598635322708791	0.0
6	0.0	0.0	0.0	4.206197130438847	0.0
7	0.0	0.0	0.0	4.971491330560359	0.0
8	0.0	0.0	0.0	6.03939804645511	0.0
9	0.0	0.0	0.0	6.386409309716315	0.0
10	0.0	0.0	0.0	8.272187689863065	0.0
11	0.0	0.0	0.0	9.469785483946348	0.0
12	0.0	0.0	0.0	11.339206430808057	0.0
13	0.0	0.0	0.0	11.808898443707061	0.0
14	0.0	0.0	0.0	12.028555404963313	0.0
15	0.0	0.0	0.0	12.473711267934757	0.0
16	0.0	0.0	0.0	13.292751320278544	0.0
17	0.0	0.0	0.0	14.149179791559565	0.0
18	0.0	0.0	0.0	15.081553488806842	0.0
19	0.0	0.0	0.0	15.642379772865356	0.0
20	0.0	0.0	0.0	16.142449876150863	0.0
21	0.0	0.0	0.0	16.7722110576249	0.0
22	0.0	0.0	0.0	17.47324391269804	0.0
23	0.0	0.0	0.0	18.154414170210778	0.0
24	0.0	0.0	0.0	18.703556573351403	0.0
25	0.0	0.0	0.0	19.15689115296537	0.0
26	0.0	0.0	0.0	19.582184418376407	0.0
27	0.0	0.0	0.0	20.048371267000046	0.0
28	0.0	0.0	0.0	20.46081226340141	0.0
29	0.0	0.0	0.0	20.964387530962284	0.0
30	0.0	0.0	0.0	21.503014441276814	0.0
31	0.0	0.0	0.0	22.07786138243679	0.0
32	0.0	0.0	0.0	22.579099873814087	0.0
33	0.0	0.0	0.0	23.011403467775857	0.0
34	0.0	0.0	0.0	23.496284525868113	0.0
35	0.0	0.0	0.0	23.98116558396037	0.0
36	0.0	0.0	0.0	24.48824601579661	0.0
37	0.0	0.0	0.0	25.015189045193253	0.0
38	0.0	0.0	0.0	25.474365565266158	0.0
39	0.0	0.0	0.0	25.92302659251297	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTATC	30	2.1419437E-6	45.000004	3
CGAAAGG	30	2.1419437E-6	45.000004	3
GGGGTAG	30	2.1419437E-6	45.000004	7
CAAAAAG	20	6.996695E-4	45.0	29
ACCTGGG	20	6.996695E-4	45.0	2
TACGGGC	20	6.996695E-4	45.0	4
GTTACGG	20	6.996695E-4	45.0	2
GCGGGAT	40	6.688424E-9	45.0	5
TTACGGG	35	1.1943848E-7	45.0	3
GCTACAC	20	6.996695E-4	45.0	9
GGATTGA	20	6.996695E-4	45.0	8
ATTGCGG	20	6.996695E-4	45.0	2
CCAGTGG	20	6.996695E-4	45.0	2
AGGGAGC	25	3.8605078E-5	45.0	6
CTATCTC	55	6.002665E-11	40.90909	5
CGGGGGA	50	1.0568328E-9	40.5	6
AGGGGCC	45	1.8926585E-8	40.000004	6
ATGGGAC	40	3.4084587E-7	39.375	5
ATGAAAC	70	0.0	38.571426	3
GGTAGGG	35	6.1807623E-6	38.571426	3
>>END_MODULE