##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936312.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	122466
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93087060898535	31.0	31.0	34.0	30.0	34.0
2	32.04338346969771	33.0	31.0	34.0	30.0	34.0
3	31.961328042068818	33.0	31.0	34.0	30.0	34.0
4	35.70902944490716	37.0	35.0	37.0	35.0	37.0
5	35.784527950614866	37.0	35.0	37.0	35.0	37.0
6	35.76244018748061	37.0	35.0	37.0	35.0	37.0
7	26.87011905345157	35.0	11.0	37.0	0.0	37.0
8	31.145656753711233	35.0	19.0	37.0	17.0	37.0
9	35.881893750102066	39.0	32.0	39.0	32.0	39.0
10	36.617355020985414	38.0	35.0	39.0	32.0	39.0
11	36.932364901278724	39.0	37.0	39.0	33.0	39.0
12	36.957865856645924	39.0	37.0	39.0	33.0	39.0
13	36.920769846324696	39.0	37.0	39.0	33.0	39.0
14	38.09107834011073	40.0	37.0	41.0	33.0	41.0
15	38.163114660395536	40.0	37.0	41.0	33.0	41.0
16	38.119772018356116	40.0	37.0	41.0	33.0	41.0
17	37.980198585729916	40.0	37.0	41.0	33.0	41.0
18	37.87998301569415	40.0	36.0	41.0	33.0	41.0
19	37.76186860026456	40.0	36.0	41.0	33.0	41.0
20	37.84970522430716	40.0	36.0	41.0	33.0	41.0
21	37.76088057093397	40.0	36.0	41.0	33.0	41.0
22	37.77480280241046	40.0	36.0	41.0	33.0	41.0
23	37.774917119853676	39.0	36.0	41.0	33.0	41.0
24	37.717096990185034	40.0	36.0	41.0	33.0	41.0
25	37.60623356686754	39.0	36.0	41.0	33.0	41.0
26	37.46210376757631	39.0	36.0	41.0	33.0	41.0
27	37.3153773292179	39.0	36.0	41.0	32.0	41.0
28	37.22067349305113	39.0	35.0	41.0	32.0	41.0
29	37.21961197393562	39.0	36.0	41.0	32.0	41.0
30	37.15842764522398	39.0	35.0	41.0	32.0	41.0
31	37.01686182287329	39.0	35.0	41.0	31.0	41.0
32	36.9009521009913	39.0	35.0	41.0	31.0	41.0
33	36.80160207731125	39.0	35.0	41.0	31.0	41.0
34	36.689415837865205	39.0	35.0	41.0	30.0	41.0
35	36.603694086521976	39.0	35.0	41.0	30.0	41.0
36	36.42059837015988	39.0	35.0	40.0	30.0	41.0
37	36.33958813058318	39.0	35.0	40.0	30.0	41.0
38	36.18068688452305	39.0	35.0	40.0	30.0	41.0
39	36.19229010500874	39.0	35.0	40.0	30.0	41.0
40	36.0107295085983	38.0	35.0	40.0	29.0	41.0
41	35.93456142929466	38.0	35.0	40.0	29.0	41.0
42	35.84444662191955	38.0	35.0	40.0	29.0	41.0
43	35.79333039374194	38.0	35.0	40.0	28.0	41.0
44	35.5806427906521	38.0	34.0	40.0	28.0	41.0
45	35.4851713945095	38.0	34.0	40.0	27.0	41.0
46	35.35452288798524	38.0	34.0	40.0	27.0	41.0
47	35.18374895889471	38.0	34.0	40.0	26.0	41.0
48	35.05136119412735	38.0	34.0	40.0	26.0	41.0
49	34.86774288373916	37.0	34.0	40.0	25.0	41.0
50	34.61420312576552	37.0	34.0	40.0	24.0	41.0
51	32.25629154214231	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	7.0
11	3.0
12	2.0
13	2.0
14	4.0
15	8.0
16	11.0
17	28.0
18	59.0
19	108.0
20	155.0
21	270.0
22	356.0
23	546.0
24	693.0
25	892.0
26	1158.0
27	1412.0
28	1744.0
29	2178.0
30	2622.0
31	3403.0
32	4361.0
33	5994.0
34	8836.0
35	11049.0
36	12119.0
37	18599.0
38	22976.0
39	22857.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.40388352685643	20.143550046543528	24.25979455522349	12.192771871376545
2	29.595969493573726	26.720069243708455	26.082341221236916	17.6016200414809
3	30.788137115607594	25.60710727875492	26.1492985808306	17.455457024806886
4	28.145771071154442	24.218150343768883	28.43156467917626	19.204513905900413
5	26.077441902242253	28.175166985122402	25.581794130615844	20.165596982019498
6	26.265249130370876	34.87253605082227	24.55946956706351	14.30274525174334
7	60.535985498015776	27.41332288145281	7.581696144235951	4.468995476295461
8	81.11557493508403	6.01473061911061	8.252902846504336	4.61679159930103
9	75.48625741021998	7.169336795518756	9.614096973853968	7.730308820407298
10	43.740303431156406	23.8751980141427	16.80139793901981	15.583100615681087
11	29.355902862835396	24.47291493149119	24.67133735077491	21.4998448548985
12	26.79110936913102	21.27120996848105	29.818071954664973	22.11960870772296
13	23.252984501820915	23.610634788431074	32.146065030294125	20.99031567945389
14	19.504188917740432	26.662093968938315	31.72635670308494	22.10736041023631
15	18.359381379321608	24.755442326849902	35.97814903728382	20.907027256544673
16	21.682752764032468	25.031437296882398	30.397008149200595	22.88880178988454
17	22.384988486600363	23.831104143190764	28.66591543775415	25.11799193245472
18	23.331373605735468	23.757614358270867	30.372511554227295	22.53850048176637
19	24.882824620711055	25.63813629905443	26.822138389430535	22.656900690803976
20	26.070909476916043	24.88527428020838	27.952247970865386	21.091568272010193
21	25.659366681364627	24.637858670978066	29.85400029395914	19.848774353698172
22	23.85151797233518	22.667515881959073	28.44217987033136	25.03878627537439
23	22.590759884376073	25.14738784642268	28.52955105906946	23.732301210131794
24	24.33246778697761	22.42663269805497	28.550781441379648	24.690118073587772
25	21.84606339718779	26.687407117077395	27.645223980533373	23.821305505201444
26	22.21351232178727	26.46857086864926	26.551859291558472	24.766057518004995
27	22.845524472098376	26.462038443323046	27.726879297111033	22.965557787467542
28	20.24970195809449	24.722780200218835	31.22009374030343	23.80742410138324
29	22.014273349337778	23.86050005715872	29.511864517498736	24.61336207600477
30	23.069260039521172	24.98571031959891	28.343376937272385	23.60165270360753
31	23.31749220191727	24.776672709160096	28.00859013930397	23.89724494961867
32	24.858328025737755	25.573628598958077	27.514575474009114	22.053467901295054
33	22.909215619028956	23.983799585190994	26.69148988290628	26.415494912873775
34	21.739094932471055	23.937256054741724	30.223898878055948	24.099750134731273
35	22.498489376643313	25.21679486551369	27.410873221955484	24.873842535887512
36	24.428821060539253	23.071709699018502	28.5491483350481	23.95032090539415
37	23.48570215406725	24.481897016314733	29.767445658386816	22.2649551712312
38	21.23936439501576	25.915764375418483	26.89154540852155	25.95332582104421
39	22.539317034932143	23.51019874904055	29.616383322718143	24.334100893309163
40	22.67078209462218	23.969101628207014	28.885568239348064	24.474548037822743
41	21.305505201443665	24.64684075580161	27.444351901752324	26.6033021410024
42	22.495223163980206	24.287557362859896	27.96939558734669	25.247823885813204
43	23.11825322946777	23.596753384612875	28.159652474972646	25.125340910946715
44	22.084496921594564	23.095389740826025	28.953342152107524	25.866771185471887
45	21.552920810673985	23.211340290366305	28.03961915960348	27.196119739356227
46	23.69392321134029	23.86539937615338	28.13270622050202	24.30797119200431
47	21.21078503421358	23.544493982003168	30.99799127921219	24.246729704571067
48	21.98324432903826	23.58205542762889	29.29792758806526	25.136772655267585
49	21.552104257508205	22.003658158182677	31.17436676301994	25.269870821289175
50	21.148726993614556	23.47835317557526	29.620466088547026	25.75245374226316
51	20.911110022373556	23.72985155063446	27.90652099358189	27.45251743341009
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	47.0
1	142.0
2	237.0
3	374.0
4	511.0
5	369.0
6	227.0
7	213.5
8	200.0
9	207.0
10	214.0
11	229.0
12	244.0
13	225.5
14	207.0
15	221.5
16	236.0
17	213.0
18	190.0
19	205.0
20	220.0
21	262.0
22	304.0
23	413.5
24	523.0
25	629.5
26	790.0
27	844.0
28	1087.0
29	1330.0
30	1464.5
31	1599.0
32	1822.0
33	2045.0
34	2378.0
35	2711.0
36	3277.5
37	3844.0
38	4018.5
39	4193.0
40	4785.0
41	5377.0
42	6105.0
43	6833.0
44	7863.5
45	8894.0
46	10946.0
47	12998.0
48	12178.0
49	11358.0
50	10736.0
51	10114.0
52	9042.5
53	7971.0
54	7539.0
55	7107.0
56	6844.5
57	6582.0
58	6190.5
59	5799.0
60	5387.0
61	4975.0
62	4559.0
63	4143.0
64	3625.0
65	3107.0
66	2663.0
67	2219.0
68	1885.5
69	1552.0
70	1303.0
71	1054.0
72	932.5
73	811.0
74	646.0
75	349.5
76	218.0
77	155.5
78	93.0
79	75.5
80	58.0
81	44.5
82	31.0
83	19.5
84	8.0
85	8.0
86	8.0
87	6.0
88	4.0
89	4.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	122466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.4055329642513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.91078812619872	32.442473829471034
2	10.159947915750207	9.429555958388452
3	5.321039573472225	7.40777031992553
4	3.617743836990375	6.715333235346953
5	2.6482025655012227	6.144562572469094
6	1.9760342066829724	5.50193523100289
7	1.5027009906565079	4.881354825012656
8	1.226443314388274	4.553100452370454
9	1.0047333321602645	4.196266718926069
>10	2.6006932835952212	15.317720836803684
>50	0.0052788091006668895	0.17392582431042083
>100	0.021115236402667558	1.6306566720559177
>500	0.0052788091006668895	1.6053435239168423
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG	762	0.6222135123217872	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	668	0.5454575147387847	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC	536	0.43767249685627035	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	287	0.234350758577891	TruSeq Adapter, Index 23 (95% over 23bp)
GCCCTGCTAGAGGACTTTCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	223	0.1820913559681871	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	193	0.15759476099488837	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG	181	0.1477961230055689	No Hit
GAATCTATCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	181	0.1477961230055689	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT	163	0.13309816602158966	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT	145	0.11840020903761043	No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	133	0.10860157104829096	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTC	129	0.10533535838518446	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC	124	0.10125259255630134	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14942922933712213	0.0
2	0.0	0.0	0.0	0.8173697189424003	0.0
3	0.0	0.0	0.0	1.0427383926967484	0.0
4	0.0	0.0	0.0	1.4012052324726862	0.0
5	0.0	0.0	0.0	2.7338199990201364	0.0
6	0.0	0.0	0.0	3.2604967909460587	0.0
7	0.0	0.0	0.0	3.825551581663482	0.0
8	0.0	0.0	0.0	4.695997256381363	0.0
9	0.0	0.0	0.0	4.984240523900511	0.0
10	0.0	0.0	0.0	6.471183838779743	0.0
11	0.0	0.0	0.0	7.513105678310715	0.0
12	0.0	0.0	0.0	8.86613427400258	0.0
13	0.0	0.0	0.0	9.2531804745807	0.0
14	0.0	0.0	0.0	9.437721490046217	0.0
15	0.0	0.0	0.0	9.738213055052015	0.0
16	0.0	0.0	0.0	10.320415462250747	0.0
17	0.0	0.0	0.0	10.911599954273022	0.0
18	0.0	0.0	0.0	11.62363431483024	0.0
19	0.0	0.0	0.0	12.075188215504712	0.0
20	0.0	0.0	0.0	12.49897930854278	0.0
21	0.0	0.0	0.0	13.010141590318945	0.0
22	0.0	0.0	0.0	13.524570084758219	0.0
23	0.0	0.0	0.0	14.021034409550406	0.0
24	0.0	0.0	0.0	14.45544069374357	0.0
25	0.0	0.0	0.0	14.870249701958095	0.0
26	0.0	0.0	0.0	15.20503649992651	0.0
27	0.0	0.0	0.0	15.552888148547352	0.0
28	8.165531657766237E-4	0.0	0.0	15.92605294530727	0.0
29	8.165531657766237E-4	0.0	0.0	16.281253572420102	0.0
30	8.165531657766237E-4	0.0	0.0	16.739339898420788	0.0
31	8.165531657766237E-4	0.0	0.0	17.121486780004247	0.0
32	8.165531657766237E-4	0.0	0.0	17.484036385609066	0.0
33	8.165531657766237E-4	0.0	0.0	17.866183267192525	0.0
34	8.165531657766237E-4	0.0	0.0	18.26629431842307	0.0
35	8.165531657766237E-4	0.0	0.0	18.66558881648784	0.0
36	8.165531657766237E-4	0.0	0.0	19.049368804402853	0.0
37	8.165531657766237E-4	0.0	0.0	19.43069913282054	0.0
38	8.165531657766237E-4	0.0	0.0	19.82509431189065	0.0
39	8.165531657766237E-4	0.0	0.0	20.21867293779498	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGCGAT	30	2.1495925E-6	45.000004	7
ATGGGCG	20	7.008595E-4	45.0	5
CACGGGA	25	3.870354E-5	45.0	4
TTTCGTC	50	2.1827873E-11	45.0	16
TAAGTAT	20	7.008595E-4	45.0	30
TACGGCT	55	1.8189894E-12	45.0	7
ATCCGGT	20	7.008595E-4	45.0	43
AATGCCG	20	7.008595E-4	45.0	17
TCGTGTC	20	7.008595E-4	45.0	31
TGATTGG	25	3.870354E-5	45.0	2
GTATCTA	50	2.1827873E-11	45.0	24
CTTTCGT	50	2.1827873E-11	45.0	15
GGACCAC	25	3.870354E-5	45.0	8
GGCGATT	35	1.2000783E-7	45.0	8
TCCTTAA	20	7.008595E-4	45.0	23
CGTTTTA	35	1.2000783E-7	45.0	1
GCCGTTA	20	7.008595E-4	45.0	44
GTAGTAG	25	3.870354E-5	45.0	1
TGCCGTT	20	7.008595E-4	45.0	43
TATCTCT	60	3.6379788E-12	41.250004	6
>>END_MODULE