##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936307.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	118638
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98568755373489	33.0	31.0	34.0	30.0	34.0
2	32.09325005478852	33.0	31.0	34.0	30.0	34.0
3	31.963611996156374	33.0	31.0	34.0	30.0	34.0
4	35.7353040341206	37.0	35.0	37.0	35.0	37.0
5	35.80293835027563	37.0	35.0	37.0	35.0	37.0
6	35.78046662957905	37.0	35.0	37.0	35.0	37.0
7	26.828756384969402	35.0	11.0	37.0	0.0	37.0
8	31.115974645560446	35.0	19.0	37.0	17.0	37.0
9	35.833282759318266	39.0	32.0	39.0	32.0	39.0
10	36.6175087240176	38.0	35.0	39.0	33.0	39.0
11	36.97612063588395	39.0	37.0	39.0	34.0	39.0
12	37.0145231713279	39.0	37.0	39.0	33.0	39.0
13	36.992869063874984	39.0	37.0	39.0	33.0	39.0
14	38.22116859690824	40.0	37.0	41.0	33.0	41.0
15	38.30479273082823	40.0	37.0	41.0	34.0	41.0
16	38.25483403294054	40.0	37.0	41.0	34.0	41.0
17	38.12034929786409	40.0	37.0	41.0	33.0	41.0
18	38.01979972690032	40.0	37.0	41.0	33.0	41.0
19	37.93239097085251	40.0	37.0	41.0	33.0	41.0
20	37.966949881151066	40.0	37.0	41.0	33.0	41.0
21	37.87021864832516	40.0	36.0	41.0	33.0	41.0
22	37.86887000792326	40.0	36.0	41.0	33.0	41.0
23	37.850739223520286	40.0	36.0	41.0	33.0	41.0
24	37.821591732834335	40.0	36.0	41.0	33.0	41.0
25	37.70501019909304	40.0	36.0	41.0	33.0	41.0
26	37.56330180886394	40.0	36.0	41.0	33.0	41.0
27	37.45889175474974	40.0	36.0	41.0	33.0	41.0
28	37.267342672668114	39.0	36.0	41.0	32.0	41.0
29	37.17931859943694	39.0	36.0	41.0	32.0	41.0
30	37.121478784200676	39.0	36.0	41.0	31.0	41.0
31	36.97306090797215	39.0	35.0	41.0	31.0	41.0
32	36.83587889209191	39.0	35.0	41.0	31.0	41.0
33	36.683642677725516	39.0	35.0	41.0	30.0	41.0
34	36.5377956472631	39.0	35.0	41.0	30.0	41.0
35	36.41755592643167	39.0	35.0	41.0	30.0	41.0
36	36.26382777862068	39.0	35.0	41.0	30.0	41.0
37	36.12347645779599	39.0	35.0	41.0	29.0	41.0
38	35.97701411015021	39.0	35.0	40.0	28.0	41.0
39	35.896837438257556	39.0	35.0	40.0	28.0	41.0
40	35.68027950572329	39.0	35.0	40.0	27.0	41.0
41	35.598450749338326	38.0	35.0	40.0	26.0	41.0
42	35.52205027057098	38.0	35.0	40.0	26.0	41.0
43	35.450665048298184	38.0	34.0	40.0	26.0	41.0
44	35.252414909219645	38.0	34.0	40.0	26.0	41.0
45	35.14660564068848	38.0	34.0	40.0	25.0	41.0
46	34.97856504661238	38.0	34.0	40.0	24.0	41.0
47	34.84310254724456	38.0	34.0	40.0	24.0	41.0
48	34.749161314250074	38.0	34.0	40.0	24.0	41.0
49	34.510738549200084	37.0	33.0	40.0	23.0	41.0
50	34.284318683726966	37.0	33.0	40.0	23.0	41.0
51	32.06129570626612	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	5.0
10	11.0
11	5.0
12	8.0
13	4.0
14	6.0
15	6.0
16	13.0
17	29.0
18	62.0
19	125.0
20	207.0
21	369.0
22	498.0
23	634.0
24	832.0
25	1097.0
26	1353.0
27	1614.0
28	1798.0
29	2052.0
30	2545.0
31	3124.0
32	3902.0
33	5391.0
34	8206.0
35	10049.0
36	11201.0
37	17577.0
38	22486.0
39	23418.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52536286855813	19.627775249077022	23.44442758643942	12.40243429592542
2	31.6627050354861	25.531448608371687	24.945632933798613	17.860213422343598
3	31.029686946846713	25.592980326708137	26.160252195755156	17.217080530689998
4	28.651022438004688	25.198503009153896	27.274566327820764	18.875908225020652
5	25.578651022438002	30.60739392100339	23.822889799221162	19.991065257337446
6	26.267300527655557	35.28464741482493	24.090089178846576	14.35796287867294
7	60.35502958579882	28.13853908528465	7.149479930545019	4.356951398371517
8	80.3865540551931	6.734773006962357	8.434059913349854	4.444613024494681
9	73.61385053692746	8.977730575363712	10.629815067684891	6.778603820023939
10	41.581112291171465	27.60751192703855	16.997083565130904	13.81429221665908
11	30.282034424046255	26.494040695224125	25.17911630337666	18.044808577352956
12	29.343043544226976	22.601527335255145	28.577690116151654	19.477739004366224
13	24.715521165225308	25.250762824727325	29.952460425833205	20.081255584214162
14	19.979264653820866	29.043813955056557	28.78925807919891	22.187663311923668
15	19.395977680001348	26.374348859555962	34.70136044100541	19.52831301943728
16	22.553482020937643	26.01527335255146	31.054131054131055	20.377113572379844
17	22.55095332018409	25.65451204504459	27.20207690621892	24.592457728552404
18	23.32810735177599	26.75533977309125	27.999460543839245	21.917092331293514
19	25.38984136617273	27.29648173435156	26.097034676916337	21.216642222559383
20	26.82445759368836	25.507847401338523	27.61004062779211	20.057654377181006
21	25.667155548812353	26.813499890422964	27.729732463460273	19.789612097304403
22	23.62143663918812	25.334209949594566	27.96574453379187	23.078608877425445
23	23.857448709519716	25.663783947807616	27.915170518720817	22.563596823951855
24	23.15025539877611	24.016756856993542	29.48212208567238	23.350865658557964
25	22.36551526492355	26.968593536640874	26.96775063638969	23.69814056204589
26	21.30093224767781	28.22957231241255	26.848058800721525	23.62143663918812
27	22.932787133970567	27.200391105716548	27.708659957180664	22.158161803132217
28	21.501542507459668	27.873868406412782	29.092702169625245	21.5318869165023
29	23.24466022690875	25.32830964783627	28.765656872165746	22.661373253089227
30	23.0971526829515	25.334209949594566	27.985974139820293	23.582663227633642
31	24.605101232320166	26.975336738650345	26.173738599774104	22.24582342925538
32	24.897587619481108	27.40184426574959	26.683693251740586	21.016874863028708
33	23.73775687385155	27.018324651460745	26.341475749759773	22.902442724927933
34	23.68296835752457	25.982400242755272	28.643436335744028	21.691195063976128
35	22.940373236231224	26.775569379119673	27.52406480217131	22.75999258247779
36	24.43230668082739	28.157082890810702	26.3347325477503	21.075877880611607
37	22.664744854093964	28.82971729125575	28.12252398051215	20.383013874138133
38	22.44221918778132	27.779463578280144	26.273200829413845	23.50511640452469
39	23.44442758643942	25.37129756064667	28.228729412161364	22.95554544075254
40	24.031086161263676	25.2575060267368	28.681366847047318	22.030040964952207
41	20.6367268497446	26.894418314536654	28.167197693824914	24.301657141893827
42	23.04236416662452	26.56821591732834	27.40774456750788	22.981675348539255
43	23.651781048230752	26.44430958040426	26.75533977309125	23.148569598273742
44	23.141826396264268	25.710143461622753	26.94836393061245	24.19966621150053
45	21.57908933056862	25.94278393094961	27.30828233786814	25.169844400613634
46	22.740605876700553	27.604983226285	25.925083025674738	23.729327871339706
47	22.10505908730761	26.15519479424805	29.182892496501967	22.55685362194238
48	22.412717678989868	24.71973566648123	28.98481093747366	23.882735717055244
49	21.32874795596689	25.39827036868457	28.849946897284173	24.423034778064366
50	20.16554560933259	26.21841231308687	29.571469512297917	24.044572565282625
51	21.261315935872148	25.441258281494967	27.23747871676866	26.059947065864225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	138.5
2	237.0
3	419.5
4	602.0
5	431.0
6	260.0
7	228.0
8	196.0
9	216.0
10	236.0
11	225.5
12	215.0
13	226.5
14	238.0
15	265.5
16	293.0
17	280.5
18	268.0
19	299.0
20	330.0
21	390.5
22	451.0
23	552.0
24	653.0
25	776.0
26	1115.5
27	1332.0
28	1549.0
29	1766.0
30	2098.0
31	2430.0
32	2661.5
33	2893.0
34	3348.0
35	3803.0
36	3957.5
37	4112.0
38	4447.0
39	4782.0
40	5164.5
41	5547.0
42	6162.0
43	6777.0
44	7560.0
45	8343.0
46	9346.5
47	10350.0
48	9879.0
49	9408.0
50	9033.5
51	8659.0
52	7781.5
53	6904.0
54	6548.0
55	6192.0
56	6160.0
57	6128.0
58	5974.5
59	5821.0
60	5619.0
61	5417.0
62	4869.0
63	4321.0
64	3729.5
65	3138.0
66	2615.5
67	2093.0
68	1705.5
69	1318.0
70	1067.5
71	817.0
72	681.0
73	545.0
74	441.5
75	286.5
76	235.0
77	183.5
78	132.0
79	99.5
80	67.0
81	44.0
82	21.0
83	16.5
84	12.0
85	13.0
86	14.0
87	8.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	118638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.54547446855139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.5392415834914	33.06276235270318
2	10.124629921818213	9.627606669026788
3	5.73155813994717	8.175289536236281
4	3.682167106919354	7.002815286838955
5	2.8010707890864612	6.658911984355771
6	2.06889215877462	5.9019875587922925
7	1.5813640151045083	5.263069168394612
8	1.2427535589554488	4.726984608641414
9	0.9218713989398479	3.9447731755424065
>10	2.276313223536086	13.74938889731789
>50	0.017728296133458613	0.5402990610091202
>100	0.012409807293421029	1.3461117011412869
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG	290	0.24444107284343972	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTC	281	0.2368549705827812	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	249	0.20988216254488445	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	214	0.18038065375343482	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGC	210	0.17700905274869771	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTT	195	0.1643655489809336	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	158	0.13317823968711542	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1070483319004029	0.0
2	0.0	0.0	0.0	0.5394561607579359	0.0
3	0.0	0.0	0.0	0.6953927072270267	0.0
4	0.0	0.0	0.0	0.941519580572835	0.0
5	0.0	0.0	0.0	1.8282506448186921	0.0
6	0.0	0.0	0.0	2.3811932095955766	0.0
7	0.0	0.0	0.0	2.738582916097709	0.0
8	0.0	0.0	0.0	3.3842445085048634	0.0
9	0.0	0.0	0.0	3.612670476575802	0.0
10	0.0	0.0	0.0	4.493501239063369	0.0
11	0.0	0.0	0.0	5.1855223452856585	0.0
12	0.0	0.0	0.0	6.013250391948617	0.0
13	0.0	0.0	0.0	6.360525295436538	0.0
14	0.0	0.0	0.0	6.570407457981423	0.0
15	0.0	0.0	0.0	6.839292638109206	0.0
16	0.0	0.0	0.0	7.302044876009373	0.0
17	0.0	0.0	0.0	7.796827323454543	0.0
18	0.0	0.0	0.0	8.39191490079064	0.0
19	0.0	0.0	0.0	8.69367319071461	0.0
20	0.0	0.0	0.0	9.035890692695427	0.0
21	0.0	0.0	0.0	9.43289671100322	0.0
22	0.0	0.0	0.0	9.848446534837068	0.0
23	0.0	0.0	0.0	10.224380046865255	0.0
24	0.0	0.0	0.0	10.543839242064093	0.0
25	0.0	0.0	0.0	10.843911731485695	0.0
26	0.0	0.0	0.0	11.12291171462769	0.0
27	0.0	0.0	0.0	11.389268194001922	0.0
28	0.0	0.0	0.0	11.695240985181814	0.0
29	0.0	0.0	0.0	12.002899576864074	0.0
30	0.0	0.0	0.0	12.325730373067652	0.0
31	0.0	0.0	0.0	12.63676056575465	0.0
32	0.0	0.0	0.0	12.963805863214148	0.0
33	0.0	0.0	0.0	13.260506751631011	0.0
34	0.0	0.0	0.0	13.572379844569193	0.0
35	0.0	0.0	0.0	13.90111094253106	0.0
36	0.0	0.0	0.0	14.21635563647398	0.0
37	0.0	0.0	0.0	14.540029332928741	0.0
38	0.0	0.0	0.0	14.854431126620476	0.0
39	0.0	0.0	0.0	15.157875217046815	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAATCG	20	7.0076995E-4	45.0	23
TCGCAAC	25	3.869612E-5	45.0	16
TTAGCGG	25	3.869612E-5	45.0	2
TTTCGCA	25	3.869612E-5	45.0	14
TACGAAT	35	1.1996417E-7	45.0	12
AGACCTA	20	7.0076995E-4	45.0	32
CGGGATT	20	7.0076995E-4	45.0	6
CGAATAT	35	1.1996417E-7	45.0	14
TCCCGGA	20	7.0076995E-4	45.0	39
TTACGGG	40	6.726623E-9	45.0	3
ATTTGGG	20	7.0076995E-4	45.0	3
TCTATCG	20	7.0076995E-4	45.0	30
TCCGCCC	30	2.149016E-6	44.999996	35
ACGGGTT	30	2.149016E-6	44.999996	21
GAACGCA	30	2.149016E-6	44.999996	36
TTTGCCG	30	2.149016E-6	44.999996	26
CCGCCCA	30	2.149016E-6	44.999996	36
CTCCAGA	40	3.4233744E-7	39.375	17
GAATATA	40	3.4233744E-7	39.375	15
AGATTAC	40	3.4233744E-7	39.375	21
>>END_MODULE