##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936304.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219265
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.822502451371626	31.0	31.0	34.0	30.0	34.0
2	31.94370282534832	33.0	31.0	34.0	30.0	34.0
3	31.336884591704102	31.0	31.0	34.0	28.0	34.0
4	35.36318153832121	37.0	35.0	37.0	33.0	37.0
5	35.63344355004219	37.0	35.0	37.0	33.0	37.0
6	35.66736141199006	37.0	35.0	37.0	35.0	37.0
7	26.94999201879005	35.0	16.0	37.0	0.0	37.0
8	31.193815702460494	35.0	23.0	37.0	17.0	37.0
9	35.904508243449705	39.0	32.0	39.0	32.0	39.0
10	36.67221854833193	38.0	35.0	39.0	33.0	39.0
11	37.04630926048389	39.0	37.0	39.0	34.0	39.0
12	37.08070599502884	39.0	37.0	39.0	34.0	39.0
13	37.05877363008232	39.0	37.0	39.0	33.0	39.0
14	38.197601076323174	40.0	38.0	41.0	33.0	41.0
15	38.27908238889015	40.0	38.0	41.0	33.0	41.0
16	38.22506555993888	40.0	37.0	41.0	33.0	41.0
17	38.134508471484274	40.0	37.0	41.0	33.0	41.0
18	37.93027614986432	39.0	37.0	41.0	33.0	41.0
19	37.71793491893371	39.0	37.0	41.0	33.0	41.0
20	37.62727293457688	39.0	35.0	41.0	33.0	41.0
21	37.55460287779627	39.0	35.0	41.0	33.0	41.0
22	37.56922445442729	39.0	35.0	41.0	33.0	41.0
23	37.51312338950585	39.0	35.0	41.0	33.0	41.0
24	37.417686361252365	39.0	35.0	41.0	33.0	41.0
25	37.285344218183475	39.0	35.0	41.0	33.0	41.0
26	37.1620276833968	39.0	35.0	41.0	33.0	41.0
27	37.037753403415955	39.0	35.0	41.0	32.0	41.0
28	36.9748751510729	39.0	35.0	41.0	32.0	41.0
29	36.892878480377625	39.0	35.0	41.0	32.0	41.0
30	36.740984653273436	39.0	35.0	41.0	31.0	41.0
31	36.55496773310834	39.0	35.0	40.0	31.0	41.0
32	36.292385925706334	39.0	35.0	40.0	30.0	41.0
33	35.96850842587736	38.0	35.0	40.0	29.0	41.0
34	35.56803411397168	38.0	35.0	40.0	26.0	41.0
35	35.26698287460379	38.0	34.0	40.0	24.0	41.0
36	34.98126467972545	38.0	34.0	40.0	23.0	41.0
37	34.8173306273231	38.0	34.0	40.0	23.0	41.0
38	34.57887487743142	38.0	34.0	40.0	21.0	41.0
39	34.46609353978063	38.0	34.0	40.0	21.0	41.0
40	34.22090620938134	38.0	33.0	40.0	20.0	41.0
41	34.12971062413062	38.0	33.0	40.0	19.0	41.0
42	34.01862586368093	38.0	33.0	40.0	18.0	41.0
43	34.00259047271567	38.0	33.0	40.0	18.0	41.0
44	33.77415456183157	37.0	33.0	40.0	18.0	41.0
45	33.69489886666819	37.0	33.0	40.0	18.0	41.0
46	33.59811643445146	37.0	33.0	40.0	18.0	41.0
47	33.416185893781496	37.0	33.0	40.0	17.0	41.0
48	33.305009919503796	37.0	32.0	40.0	17.0	41.0
49	33.10296216906483	37.0	32.0	40.0	15.0	41.0
50	32.88650263379928	36.0	31.0	40.0	15.0	41.0
51	30.783955487651927	35.0	27.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	10.0
9	12.0
10	18.0
11	16.0
12	9.0
13	6.0
14	13.0
15	17.0
16	21.0
17	72.0
18	126.0
19	224.0
20	359.0
21	632.0
22	998.0
23	1521.0
24	2381.0
25	3760.0
26	4712.0
27	4994.0
28	4938.0
29	4962.0
30	5540.0
31	6294.0
32	7753.0
33	10503.0
34	15320.0
35	18798.0
36	20500.0
37	30531.0
38	36864.0
39	37352.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.93553462704946	18.65824458987983	21.88128520283675	20.524935580233965
2	37.215241830661526	23.46384511892003	23.147789204843455	16.173123845574988
3	29.549175655029302	22.87734020477504	31.83681846167879	15.736665678516863
4	25.88694046017376	22.939365607826147	33.61320776229677	17.560486169703328
5	23.399083301028437	27.17579185004447	30.952044329920415	18.473080519006682
6	23.524502314550887	32.01286114975031	31.100266800446946	13.362369735251864
7	54.87971176430347	27.220030556632384	13.48915695619456	4.411100722869587
8	74.23665427678836	5.458691537637106	15.989784051262172	4.314870134312362
9	68.00036485531207	6.954142247964791	17.896153056803414	7.149339839919731
10	38.82106127288897	21.68654368002189	25.33372859325474	14.158666453834401
11	29.33026246779012	23.669076231956765	29.519531160923997	17.481130139329125
12	26.054773903723806	21.12557863772148	33.8631336510615	18.956513807493216
13	23.317902994093906	21.78915923654026	35.58251430916927	19.310423460196567
14	20.00866531366155	24.019793400679543	35.56746402754658	20.40407725811233
15	19.55670079584065	23.75481723029211	38.07903678197615	18.60944519189109
16	22.557179668437737	24.082274872870727	34.250336350990814	19.110209107700726
17	23.060679999087863	23.94591019998632	32.767199507445326	20.22621029348049
18	23.00184708001733	23.7575536451326	33.881376416664764	19.3592228581853
19	23.34709141905913	25.439080564613597	31.79486010079128	19.418967915535994
20	23.42781565685358	25.76380179235172	32.083095797322876	18.725286753471828
21	22.984972521834308	25.512051627026654	33.46863384489089	18.034342006248146
22	22.440425968576836	22.727749526828266	33.309465715002396	21.522358789592502
23	21.085900622534375	23.898022940277748	33.858572959660684	21.157503477527193
24	22.25799831254418	23.134107130641006	33.54206097644403	21.065833580370786
25	22.029963742503362	25.776571728274007	31.882425375686957	20.311039153535678
26	20.53724944701617	24.89134152737555	32.209883018265565	22.361526007342714
27	20.95820126331152	24.68565434519873	32.598453925615125	21.757690465874628
28	19.62967185825371	24.46446081225914	32.938681504116026	22.967185825371125
29	21.131051467402457	23.956855859348277	32.91770232367227	21.994390349576996
30	21.298884910952502	23.662691263995622	33.18951953116092	21.848904293890953
31	21.613116548468746	23.690511481540604	32.022438601692016	22.673933368298634
32	22.867306683693247	23.75709757599252	31.26764417485691	22.107951565457324
33	22.434041000615693	24.090028048252115	30.35915444781429	23.116776503317904
34	20.807242377944497	23.99334139055481	33.442181834766146	21.757234396734546
35	21.633183590632342	24.97753859485098	31.222493329988826	22.166784484527856
36	23.4396734544957	25.759241100950902	29.594782569037463	21.206302875515927
37	22.475999361503206	25.766994276332294	30.05997309192073	21.697033270243768
38	22.247964791462387	26.046108590062257	29.35352199393428	22.35240462454108
39	23.172416938407864	24.891797596515634	29.943219392059838	21.99256607301667
40	23.712858869404602	24.387841196725425	31.14952226757576	20.74977766629421
41	22.085148108453243	25.13989920872004	30.823888901557478	21.95106378126924
42	22.37383987412492	24.94652589332543	30.6022392994778	22.077394933071854
43	22.668004469477573	24.068592798668277	30.34729665017217	22.916106081681985
44	21.812874831824505	24.837525368845917	30.243312886233554	23.106286913096024
45	20.615237269970127	24.95336693042665	29.730691172781793	24.700704626821427
46	21.68517547260165	25.988187809271885	29.457961827012973	22.868674891113493
47	20.743392698333068	24.722595945545343	31.9681663740223	22.565844982099286
48	20.945887396529315	25.05552641780494	30.8033657902538	23.195220395411944
49	21.40423688231136	24.34998745809865	31.289079424440747	22.95669623514925
50	20.316055914076575	24.61040293708526	31.813558935534626	23.259982213303537
51	20.486169703327025	24.644608122591386	30.344560235331674	24.524661938749915
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	102.0
1	229.0
2	356.0
3	3721.0
4	7086.0
5	4838.0
6	2590.0
7	2231.0
8	1872.0
9	1831.5
10	1791.0
11	1685.0
12	1579.0
13	1517.5
14	1456.0
15	1426.0
16	1396.0
17	1279.0
18	1162.0
19	1126.5
20	1091.0
21	1152.0
22	1213.0
23	1296.0
24	1379.0
25	1510.5
26	1913.0
27	2184.0
28	2481.0
29	2778.0
30	3153.0
31	3528.0
32	4080.0
33	4632.0
34	5266.0
35	5900.0
36	6320.0
37	6740.0
38	7181.5
39	7623.0
40	8289.0
41	8955.0
42	9552.0
43	10149.0
44	10872.0
45	11595.0
46	12515.5
47	13436.0
48	16070.0
49	18704.0
50	18043.5
51	17383.0
52	16641.0
53	15899.0
54	14155.0
55	12411.0
56	11301.5
57	10192.0
58	9695.0
59	9198.0
60	8682.0
61	8166.0
62	7577.5
63	6989.0
64	6154.5
65	5320.0
66	4679.5
67	4039.0
68	3597.5
69	3156.0
70	2625.5
71	2095.0
72	1727.5
73	1360.0
74	1091.5
75	655.5
76	488.0
77	387.0
78	286.0
79	257.5
80	229.0
81	188.0
82	147.0
83	113.0
84	79.0
85	56.5
86	34.0
87	25.5
88	17.0
89	12.0
90	7.0
91	6.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	219265.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.98185360549115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.54890750819824	34.26201459891118
2	12.638190614122294	12.633603862267057
3	6.349021974353179	9.520076605805011
4	3.922055025727306	7.8412632051433215
5	2.7479154541048785	6.867295397366839
6	1.7621197339431576	5.284440634637635
7	1.3157870922529558	4.603583447468748
8	0.8794979325684762	3.5167149529575767
9	0.6071996153346229	2.7314066054669093
>10	1.1925352158566187	7.60163961526876
>50	0.017888027126729805	0.6407550064164722
>100	0.014906689272274837	1.6455979350835446
>500	0.002981337854454967	0.918912218504243
>1k	9.937792848183225E-4	1.9326959147027079
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3891	1.774565024057647	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	681	0.31058308439559434	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC	668	0.3046541855745331	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG	501	0.22849063918089982	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT	434	0.1979340067954302	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGC	417	0.19018083141404235	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	378	0.17239413495085856	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC	360	0.16418489042938908	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	245	0.11173693932000092	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	224	0.10215948737828655	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1067201787791029	0.0
2	0.0	0.0	0.0	0.4145668483342075	0.0
3	0.0	0.0	0.0	0.7009782683054752	0.0
4	0.0	0.0	0.0	0.9714272683738855	0.0
5	0.0	0.0	0.0	2.0500307846669554	0.0
6	0.0	0.0	0.0	2.8513442637903905	0.0
7	0.0	0.0	0.0	3.481631815383212	0.0
8	0.0	0.0	0.0	4.419766036531138	0.0
9	0.0	0.0	0.0	4.9752582491505715	0.0
10	0.0	0.0	0.0	6.076209153307642	0.0
11	0.0	0.0	0.0	6.852438829726586	0.0
12	0.0	0.0	0.0	7.746334344286594	0.0
13	0.0	0.0	0.0	8.071967710304882	0.0
14	0.0	0.0	0.0	8.279023099901945	0.0
15	0.0	0.0	0.0	8.495655941440722	0.0
16	0.0	0.0	0.0	8.796661573894603	0.0
17	0.0	0.0	0.0	9.12229493991289	0.0
18	4.5606914008163637E-4	0.0	0.0	9.529108612865711	0.0
19	4.5606914008163637E-4	0.0	0.0	9.755318906346202	0.0
20	4.5606914008163637E-4	0.0	0.0	10.020295076733634	0.0
21	4.5606914008163637E-4	0.0	0.0	10.305794358424738	0.0
22	4.5606914008163637E-4	0.0	0.0	10.647846213485964	0.0
23	4.5606914008163637E-4	0.0	0.0	10.993546621667845	0.0
24	4.5606914008163637E-4	0.0	0.0	11.276309488518459	0.0
25	4.5606914008163637E-4	0.0	0.0	11.521218616742297	0.0
26	4.5606914008163637E-4	0.0	0.0	11.748797117643035	0.0
27	4.5606914008163637E-4	0.0	0.0	12.004651905228833	0.0
28	4.5606914008163637E-4	0.0	0.0	12.254577793993569	0.0
29	4.5606914008163637E-4	0.0	0.0	12.528675347182633	0.0
30	4.5606914008163637E-4	0.0	0.0	12.862061888582309	0.0
31	4.5606914008163637E-4	0.0	0.0	13.16580393587668	0.0
32	4.5606914008163637E-4	0.0	0.0	13.42257086174264	0.0
33	4.5606914008163637E-4	0.0	0.0	13.718559733655622	0.0
34	4.5606914008163637E-4	0.0	0.0	14.013180398148359	0.0
35	4.5606914008163637E-4	0.0	0.0	14.290014366177912	0.0
36	4.5606914008163637E-4	0.0	0.0	14.5709529564682	0.0
37	4.5606914008163637E-4	0.0	0.0	14.875151072902652	0.0
38	4.5606914008163637E-4	0.0	0.0	15.209449752582492	0.0
39	4.5606914008163637E-4	0.0	0.0	15.654573233302168	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACGG	30	2.1574415E-6	45.000004	2
CGGGTAT	40	6.7720975E-9	45.0	6
TTAGGCA	20	7.0207904E-4	45.0	5
ATCAGTA	20	7.0207904E-4	45.0	34
CTAAGCG	20	7.0207904E-4	45.0	2
TACGGGC	25	3.88045E-5	45.0	4
ACCACCT	20	7.0207904E-4	45.0	14
CGAGATA	25	3.88045E-5	45.0	19
CGCCGCA	20	7.0207904E-4	45.0	18
CGTTTTA	225	0.0	45.0	1
CGTTTTT	1445	0.0	42.664356	1
ACGGGCT	55	6.002665E-11	40.909092	5
AGGCGAT	50	1.0732037E-9	40.5	7
ATACGGG	85	0.0	39.705883	3
AGCGCAA	40	3.4411823E-7	39.375	43
AACGGGT	35	6.2251875E-6	38.57143	4
ACGCCGG	65	9.094947E-12	38.07692	27
GTAGGGT	65	9.094947E-12	38.07692	4
GGCGATC	30	1.13691116E-4	37.500004	8
ACTCATG	30	1.13691116E-4	37.500004	22
>>END_MODULE