##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936301.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128624
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98713303893519	33.0	31.0	34.0	30.0	34.0
2	32.10347991043662	33.0	31.0	34.0	30.0	34.0
3	31.971086266948625	33.0	31.0	34.0	30.0	34.0
4	35.73272484139818	37.0	35.0	37.0	35.0	37.0
5	35.81946604055231	37.0	35.0	37.0	35.0	37.0
6	35.774474437119046	37.0	35.0	37.0	35.0	37.0
7	26.707783928349297	35.0	10.0	37.0	0.0	37.0
8	31.01621781316084	35.0	19.0	37.0	17.0	37.0
9	35.82916873989302	39.0	32.0	39.0	32.0	39.0
10	36.67534052742879	38.0	35.0	39.0	33.0	39.0
11	37.03095845254385	39.0	37.0	39.0	34.0	39.0
12	37.02364255504416	39.0	37.0	39.0	33.0	39.0
13	36.897616307998504	39.0	37.0	39.0	33.0	39.0
14	38.17274381141933	40.0	37.0	41.0	33.0	41.0
15	38.23025251897002	40.0	37.0	41.0	33.0	41.0
16	38.18913266575445	40.0	37.0	41.0	33.0	41.0
17	38.03885744495584	40.0	37.0	41.0	33.0	41.0
18	37.94548451299913	40.0	37.0	41.0	33.0	41.0
19	37.80166220923	40.0	36.0	41.0	33.0	41.0
20	37.8845472073641	40.0	36.0	41.0	33.0	41.0
21	37.86726427416345	40.0	36.0	41.0	33.0	41.0
22	37.849545963428284	40.0	36.0	41.0	33.0	41.0
23	37.81747574325165	40.0	36.0	41.0	33.0	41.0
24	37.726435190944144	40.0	36.0	41.0	33.0	41.0
25	37.62378716258241	40.0	36.0	41.0	33.0	41.0
26	37.46161680557283	39.0	35.0	41.0	33.0	41.0
27	37.360158290832196	39.0	35.0	41.0	32.0	41.0
28	37.33991323547705	39.0	35.0	41.0	32.0	41.0
29	37.30657575569101	39.0	35.0	41.0	32.0	41.0
30	37.22264118671477	39.0	35.0	41.0	32.0	41.0
31	37.08731651946759	39.0	35.0	41.0	32.0	41.0
32	36.9272686279388	39.0	35.0	41.0	31.0	41.0
33	36.79311792511506	39.0	35.0	41.0	31.0	41.0
34	36.640168242318694	39.0	35.0	41.0	30.0	41.0
35	36.53419268565742	39.0	35.0	41.0	30.0	41.0
36	36.35012128374176	39.0	35.0	41.0	30.0	41.0
37	36.159402599825846	39.0	35.0	41.0	30.0	41.0
38	36.119363415847744	39.0	35.0	40.0	30.0	41.0
39	36.057376539370566	39.0	35.0	40.0	29.0	41.0
40	35.80967004602562	38.0	35.0	40.0	28.0	41.0
41	35.75948501057346	38.0	35.0	40.0	28.0	41.0
42	35.68476334121159	38.0	35.0	40.0	28.0	41.0
43	35.67764180868267	38.0	35.0	40.0	28.0	41.0
44	35.4401589128001	38.0	34.0	40.0	27.0	41.0
45	35.39047922627192	38.0	34.0	40.0	27.0	41.0
46	35.24654807811917	38.0	34.0	40.0	26.0	41.0
47	35.0258816395074	38.0	34.0	40.0	25.0	41.0
48	34.968862731683046	38.0	34.0	40.0	25.0	41.0
49	34.795769063316335	37.0	34.0	40.0	24.0	41.0
50	34.575444707053116	37.0	34.0	40.0	24.0	41.0
51	32.44661960442841	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	4.0
10	9.0
11	5.0
12	7.0
13	5.0
14	4.0
15	14.0
16	16.0
17	31.0
18	85.0
19	97.0
20	184.0
21	283.0
22	428.0
23	548.0
24	857.0
25	1121.0
26	1367.0
27	1597.0
28	1798.0
29	2186.0
30	2652.0
31	3474.0
32	4503.0
33	6104.0
34	9470.0
35	11487.0
36	12234.0
37	18707.0
38	23586.0
39	25749.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.986938673964424	19.641746485881328	22.988711282497825	12.382603557656426
2	30.619480034830204	28.67660778703819	23.904559024754324	16.799353153377286
3	31.642617240950365	27.296616494588875	25.46492101007588	15.595845254384875
4	28.423155865157355	24.11913795248165	29.487498445080234	17.970207737280756
5	27.50342082348551	29.380986441099637	23.291920636895135	19.823672098519715
6	25.27522079860679	36.921569846995894	24.322832441846	13.480376912551312
7	58.51785047891529	28.298762283866154	8.798513496703569	4.38487374051499
8	77.68923373553925	8.701331011319816	9.310082099763651	4.299353153377286
9	71.59705809180247	8.482864784177137	10.627099141684289	9.292977982336112
10	42.757183729319564	25.66472820002488	17.707426296803085	13.870661773852468
11	33.05526184848862	26.18018410250031	23.40309740017415	17.36145664883692
12	32.40997014554049	21.673249160343328	26.449962681925616	19.466818012190572
13	25.27211095907451	25.017881577310614	28.932392088568232	20.777615375046647
14	20.718528423933325	28.857755939793506	26.74850727702451	23.67520835924866
15	19.42172533897251	26.16541236472198	34.33962557532031	20.073236720985197
16	21.916594103744245	25.31331633287722	30.864379897997264	21.905709665381266
17	22.033213086204753	25.110399303395948	28.864753078741135	23.991634531658164
18	22.386179873118547	27.86338474934693	27.369697723597465	22.380737653937057
19	25.670170419206368	27.76931210349546	24.83440104490608	21.72611643239209
20	27.592828710038564	25.876974748103	27.75609528548327	18.774101256375168
21	25.65773106107725	28.54366214703321	26.353557656424933	19.445049135464608
22	23.899894265455902	24.98367334245553	25.971824853837543	25.144607538251023
23	23.82137081726583	27.917029481278767	27.449776091553673	20.81182360990173
24	24.209323298917777	24.170450304764273	28.274661027490982	23.345565368826968
25	21.921258863042667	28.79478168926483	25.488244806567984	23.79571464112452
26	21.143021520089565	28.193027739768628	26.721296181117054	23.942654559024753
27	23.762283866152504	29.25581539992536	25.493687025749473	21.48821370817266
28	20.531160592113448	27.55473317576813	29.261257619106857	22.65284861301157
29	23.42953103619853	25.572987933822617	27.325382510262468	23.672098519716382
30	22.21202885931086	27.660467719865657	29.312569971389475	20.814933449434008
31	24.05072148277149	26.42197412613509	26.334898619231247	23.19240577186217
32	27.178442592362234	27.72110959074512	24.612824978231124	20.487622838661526
33	24.27385246921259	28.608968777211096	24.77609155367583	22.341087199900485
34	23.592797611643242	28.42004602562508	26.095440975245676	21.891715387486006
35	25.102624704565248	25.066084090060954	26.620226396317946	23.211064809055852
36	24.268410250031096	28.63384749346934	25.582317452419456	21.515424804080112
37	24.35082099763652	27.80507525811668	28.251337230998878	19.592766513247916
38	23.57802587386491	28.320531160592115	25.592424430899364	22.509018534643612
39	25.45170419206369	27.317607911431768	25.803116059211344	21.427571837293197
40	25.227795745739517	26.316239582037564	27.627036944893646	20.82892772732927
41	21.167122776464733	28.70381888294564	27.11780072148277	23.011257619106853
42	23.62467346684911	25.70982087324294	28.262999129244932	22.40250653066302
43	25.88319442716756	25.1764833934569	26.295248165194675	22.645074014180867
44	22.400174151013807	27.086702326159973	27.388356760791144	23.12476676203508
45	21.879276029356884	26.557252145789278	25.685719616867768	25.877752207986067
46	24.160343326284366	29.20683542729195	24.794750590869512	21.838070655554173
47	21.96090931707924	26.207395198407763	29.999067048140315	21.832628436372683
48	23.211064809055852	25.00544221918149	27.963677074262964	23.819815897499687
49	21.367707426296803	25.263558900360746	29.992847369075754	23.3758863042667
50	20.248942654559023	26.256375171041174	28.542884687150146	24.951797487249657
51	21.56518223659659	24.98056350292325	26.39787286975992	27.05638139072024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	111.0
1	220.5
2	330.0
3	619.0
4	908.0
5	685.5
6	463.0
7	399.5
8	336.0
9	314.0
10	292.0
11	294.0
12	296.0
13	314.0
14	332.0
15	332.0
16	332.0
17	354.5
18	377.0
19	397.0
20	417.0
21	482.0
22	547.0
23	604.0
24	661.0
25	831.0
26	1176.0
27	1351.0
28	1705.0
29	2059.0
30	2292.0
31	2525.0
32	2783.5
33	3042.0
34	3557.5
35	4073.0
36	4040.5
37	4008.0
38	4317.0
39	4626.0
40	5213.5
41	5801.0
42	6548.5
43	7296.0
44	7859.0
45	8422.0
46	11052.0
47	13682.0
48	11362.0
49	9042.0
50	8626.5
51	8211.0
52	7477.5
53	6744.0
54	6341.5
55	5939.0
56	5845.5
57	5752.0
58	6056.5
59	6361.0
60	6354.0
61	6347.0
62	5675.0
63	5003.0
64	4295.5
65	3588.0
66	3064.5
67	2541.0
68	2156.0
69	1771.0
70	1550.0
71	1329.0
72	1136.0
73	943.0
74	839.0
75	573.5
76	412.0
77	325.0
78	238.0
79	212.5
80	187.0
81	140.0
82	93.0
83	73.0
84	53.0
85	40.5
86	28.0
87	18.0
88	8.0
89	5.0
90	2.0
91	4.0
92	6.0
93	3.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	128624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.09208234855082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.78163736709396	29.886335365095164
2	10.482605399114842	9.243998009702699
3	6.093840918307972	8.060704067670109
4	4.023768800804048	7.096653812663267
5	2.8917532135489217	6.3751710411742755
6	2.1811577592439124	5.770307252145789
7	1.685680531800469	5.2027615375046645
8	1.2483910214589249	4.40353277770867
9	1.0050605681237106	3.988369200149272
>10	2.5461534392467335	14.515953476800597
>50	0.03702854724666302	1.1296492101007587
>100	0.01763264154603001	1.802929468839408
>500	0.0	0.0
>1k	0.005289792463809003	2.523634780445329
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC	1133	0.8808620475183481	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG	1080	0.8396566737156363	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC	1033	0.8031160592113448	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	370	0.28766015673591244	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT	270	0.2099141684289091	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG	257	0.1998071899489986	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC	256	0.19902973006592858	No Hit
GAATCTATCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC	247	0.1920325911182983	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	236	0.1834805324045279	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	208	0.161711655678567	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTC	192	0.14927229754944643	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT	172	0.1337230998880458	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08863042666998383	0.0
2	0.0	0.0	0.0	0.49912924493096156	0.0
3	0.0	0.0	0.0	0.6033088692623461	0.0
4	0.0	0.0	0.0	0.9189575817887797	0.0
5	0.0	0.0	0.0	2.346373927105361	0.0
6	0.0	0.0	0.0	2.662800099514865	0.0
7	0.0	0.0	0.0	3.148712526433636	0.0
8	0.0	0.0	0.0	3.8002239084463243	0.0
9	0.0	0.0	0.0	3.9969212588630425	0.0
10	0.0	0.0	0.0	5.519187709914169	0.0
11	0.0	0.0	0.0	6.149707675083966	0.0
12	0.0	0.0	0.0	7.769934071401916	0.0
13	0.0	0.0	0.0	8.059149147903968	0.0
14	0.0	0.0	0.0	8.185875108844384	0.0
15	0.0	0.0	0.0	8.475090185346437	0.0
16	0.0	0.0	0.0	8.844383629804701	0.0
17	0.0	0.0	0.0	9.216786913795248	0.0
18	0.0	0.0	0.0	9.670046025625078	0.0
19	0.0	0.0	0.0	9.92738524692126	0.0
20	0.0	0.0	0.0	10.18705684786665	0.0
21	0.0	0.0	0.0	10.516699838288345	0.0
22	0.0	0.0	0.0	10.859559646722229	0.0
23	0.0	0.0	0.0	11.231185470829706	0.0
24	0.0	0.0	0.0	11.498631670605796	0.0
25	0.0	0.0	0.0	11.731092175643736	0.0
26	0.0	0.0	0.0	11.957333001617117	0.0
27	0.0	0.0	0.0	12.178909068292077	0.0
28	0.0	0.0	0.0	12.402817514616245	0.0
29	0.0	0.0	0.0	12.675705933573827	0.0
30	0.0	0.0	0.0	12.94703943276527	0.0
31	0.0	0.0	0.0	13.240141808682672	0.0
32	0.0	0.0	0.0	13.498258489861923	0.0
33	0.0	0.0	0.0	13.748600572210474	0.0
34	0.0	0.0	0.0	14.012159472571215	0.0
35	0.0	0.0	0.0	14.274940913048887	0.0
36	0.0	0.0	0.0	14.557158850603308	0.0
37	0.0	0.0	0.0	14.826159970145541	0.0
38	0.0	0.0	0.0	15.112265207115312	0.0
39	0.0	0.0	0.0	15.454347555666128	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGGT	35	1.2007149E-7	45.000004	4
GCTCTCG	20	7.0099137E-4	45.000004	9
CTACGGG	20	7.0099137E-4	45.000004	3
CTACGAA	35	1.2007149E-7	45.000004	11
TACGAAT	35	1.2007149E-7	45.000004	12
CGGGCAA	20	7.0099137E-4	45.000004	6
CGAATAT	35	1.2007149E-7	45.000004	14
GGTTGTA	20	7.0099137E-4	45.000004	8
ACGTAAG	20	7.0099137E-4	45.000004	1
GCTACGA	35	1.2007149E-7	45.000004	10
CCTATGC	20	7.0099137E-4	45.000004	35
CGTTTTA	35	1.2007149E-7	45.000004	1
TATCGGG	20	7.0099137E-4	45.000004	3
GACCTAT	20	7.0099137E-4	45.000004	33
GTTGATC	55	1.8189894E-12	45.0	16
CATATGC	55	1.8189894E-12	45.0	33
CTATCTC	25	3.8714465E-5	45.0	5
GGGTCTC	25	3.8714465E-5	45.0	7
GCGCGAC	25	3.8714465E-5	45.0	9
TAGCATA	55	1.8189894E-12	45.0	30
>>END_MODULE