##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936298.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	299147
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.560139329493527	31.0	31.0	34.0	30.0	34.0
2	31.68448622249262	31.0	31.0	34.0	30.0	34.0
3	31.6372519196248	31.0	31.0	34.0	30.0	34.0
4	35.41269008213353	37.0	35.0	37.0	33.0	37.0
5	30.90405720264619	35.0	35.0	37.0	0.0	37.0
6	33.14712499206076	36.0	35.0	37.0	17.0	37.0
7	29.624358592932573	35.0	32.0	37.0	0.0	37.0
8	32.61186974965485	35.0	33.0	37.0	17.0	37.0
9	36.33616583151427	39.0	35.0	39.0	32.0	39.0
10	36.49293156876051	38.0	35.0	39.0	32.0	39.0
11	36.6485373411734	38.0	35.0	39.0	32.0	39.0
12	36.643088515011016	38.0	35.0	39.0	33.0	39.0
13	36.354681811951984	39.0	35.0	39.0	32.0	39.0
14	37.48436053177869	39.0	36.0	41.0	32.0	41.0
15	37.61780328734702	39.0	36.0	41.0	33.0	41.0
16	37.690058733666056	39.0	36.0	41.0	33.0	41.0
17	37.65479847700294	39.0	36.0	41.0	33.0	41.0
18	37.59067949870799	39.0	36.0	41.0	33.0	41.0
19	37.48741588583539	39.0	36.0	41.0	32.0	41.0
20	37.409236930338594	39.0	36.0	40.0	32.0	41.0
21	37.316954540744184	39.0	35.0	40.0	32.0	41.0
22	37.28595974554316	39.0	35.0	40.0	32.0	41.0
23	37.25382169969948	39.0	35.0	40.0	32.0	41.0
24	37.21320955917994	39.0	35.0	40.0	32.0	41.0
25	37.08704750507275	39.0	35.0	40.0	32.0	41.0
26	36.91434980126827	39.0	35.0	40.0	31.0	41.0
27	36.87264789551625	39.0	35.0	40.0	31.0	41.0
28	36.777600978783006	39.0	35.0	40.0	31.0	41.0
29	36.78006130765142	39.0	35.0	40.0	31.0	41.0
30	36.644642934744454	39.0	35.0	40.0	31.0	41.0
31	36.51982804440626	38.0	35.0	40.0	30.0	41.0
32	36.436370747492035	38.0	35.0	40.0	30.0	41.0
33	36.279177126964335	38.0	35.0	40.0	30.0	41.0
34	36.138530555211986	38.0	35.0	40.0	30.0	41.0
35	36.00664890505337	38.0	35.0	40.0	29.0	41.0
36	35.86526356607287	38.0	35.0	40.0	29.0	41.0
37	35.72818714545023	38.0	35.0	40.0	28.0	41.0
38	35.64024710259505	38.0	34.0	40.0	28.0	41.0
39	35.65920099482863	38.0	35.0	40.0	28.0	41.0
40	35.483722049694634	38.0	34.0	40.0	27.0	41.0
41	35.41623683339629	38.0	34.0	40.0	27.0	41.0
42	35.34377078827466	38.0	34.0	40.0	27.0	41.0
43	35.09415437895082	38.0	34.0	40.0	26.0	41.0
44	34.94099556405379	38.0	34.0	40.0	25.0	41.0
45	34.789558310797034	38.0	34.0	40.0	24.0	41.0
46	34.60746723182917	37.0	33.0	40.0	24.0	41.0
47	34.417400141067766	37.0	33.0	40.0	23.0	41.0
48	34.28930258367959	37.0	33.0	40.0	23.0	41.0
49	34.049273434131045	37.0	33.0	40.0	23.0	41.0
50	33.82675407074114	37.0	33.0	40.0	22.0	41.0
51	31.53749494395732	35.0	29.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	14.0
10	19.0
11	8.0
12	6.0
13	7.0
14	9.0
15	11.0
16	25.0
17	56.0
18	135.0
19	257.0
20	453.0
21	650.0
22	1063.0
23	1568.0
24	2140.0
25	2943.0
26	3524.0
27	4441.0
28	5141.0
29	6493.0
30	7971.0
31	9909.0
32	12739.0
33	17719.0
34	24647.0
35	29572.0
36	32037.0
37	45181.0
38	50300.0
39	40101.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.353582018205095	22.08746870267795	26.857030155742827	9.701919123374127
2	29.823799001828533	24.30243325187951	28.304144785005363	17.56962296128659
3	30.1781398442906	24.446843859373484	28.47429524614989	16.90072105018603
4	27.393889960454228	26.8814328741388	27.241122257619168	18.48355490778781
5	21.019431918087093	39.06273504330647	23.398195535973954	16.519637502632484
6	24.109885775220878	35.08542622857659	26.183782555064898	14.620905441137635
7	68.96776501185036	18.714879306829086	7.670810671676468	4.646545009644088
8	82.04996205878714	4.236378770303563	8.228396072833757	5.485263098075528
9	76.87959431316375	6.402537882713181	10.434000675253303	6.283867128869754
10	39.664445907864696	25.226059428976388	18.90876391874229	16.200730744416624
11	29.4243298445246	25.42428973046696	25.130788542088005	20.02059188292044
12	26.928566891862527	23.45602663573427	30.459606815378393	19.155799657024808
13	22.554463190337863	27.33472172543933	31.120485914951512	18.990329169271295
14	18.672425262496365	31.040592083490726	29.446058292411426	20.840924361601488
15	17.82936148448756	27.326030346284604	36.3801074388177	18.464500730410133
16	20.920818193062274	25.791333357847478	32.47500392783481	20.812844521255435
17	20.4518180025205	25.51421207633705	27.762270723089316	26.271699198053135
18	22.24424781127673	26.16706836438273	30.922589897274584	20.66609392706596
19	22.293721815695964	30.086880363165935	26.055751854439457	21.563645966698648
20	23.70205952257586	28.254336496772492	27.44269539724617	20.600908583405484
21	21.936038135097462	29.385552922141954	27.965849565598184	20.7125593771624
22	20.950235168662896	26.452546741234244	26.09987731784039	26.49734077226247
23	18.60824277027682	28.36732442578398	27.741879410457067	25.282553393482132
24	21.111359966839046	25.84415020040315	30.129000123685014	22.915489709072798
25	18.93985231341113	28.49802939691857	27.829127485818013	24.732990803852285
26	18.403995360140666	29.038900607393693	28.795207707247606	23.76189632521804
27	20.135585514813787	28.378021507820574	28.968701006528562	22.51769197083708
28	18.53169177695247	27.530946324048045	30.293133476183947	23.644228422815537
29	18.735270619461335	25.17090259972522	30.017015046114455	26.076811734698996
30	20.83457296914226	26.717968089267153	29.06062905528051	23.386829886310075
31	22.098834352341825	28.200851086589534	25.467078058613325	24.233236502455316
32	20.98934637485918	28.597980257197964	24.89645558872394	25.516217779218913
33	20.96661507553143	27.543314825152855	26.346578772309265	25.143491327006455
34	19.011723333344477	26.605314444069307	28.302807649750793	26.08015457283543
35	18.209442180600174	26.239942235757002	28.079840346050606	27.47077523759222
36	21.361738543257996	28.215559574389847	26.960992421785946	23.46170946056621
37	19.760519075905826	27.83848743260003	30.199199724550134	22.20179376694401
38	18.31240159520236	30.411135662400092	24.902806981183165	26.373655761214387
39	19.20928506720776	27.67836548586481	28.145694257338366	24.966655189589066
40	19.42757239751694	26.829284599210425	29.60116598194199	24.14197702133065
41	17.658876739529394	28.463932447926936	27.57808034177177	26.299110470771897
42	20.608931394932924	27.312993277552504	27.932421184233835	24.14565414328073
43	21.048514609874076	26.52976630218588	27.549666217612078	24.872052870327966
44	20.118202756504328	26.410092696901522	26.581246009486975	26.890458537107175
45	19.754836251073886	25.587754515338613	26.606651579323877	28.050757654263624
46	21.657245434518817	26.696908209007614	27.166911250990317	24.478935105483256
47	17.972100672913317	27.072308931729218	29.759282225795346	25.19630816956212
48	19.487743483972764	25.60981724703908	29.005806509842984	25.89663275914517
49	19.966437905110197	24.67749969078747	29.9073699552394	25.448692448862936
50	18.507289058556495	23.793653287514164	30.460609666819323	27.238447987110014
51	18.526008952120527	24.894784169655722	26.960992421785946	29.6182144564378
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	267.0
1	320.5
2	374.0
3	741.0
4	1108.0
5	912.5
6	717.0
7	667.5
8	618.0
9	624.0
10	630.0
11	644.5
12	659.0
13	679.5
14	700.0
15	774.0
16	848.0
17	847.5
18	847.0
19	944.0
20	1041.0
21	1239.5
22	1438.0
23	1537.0
24	1636.0
25	1689.0
26	1915.5
27	2089.0
28	2636.5
29	3184.0
30	3742.5
31	4301.0
32	5177.5
33	6054.0
34	7241.5
35	8429.0
36	8925.5
37	9422.0
38	10422.0
39	11422.0
40	13524.0
41	15626.0
42	17781.5
43	19937.0
44	22419.5
45	24902.0
46	28899.0
47	32896.0
48	34209.0
49	35522.0
50	33310.0
51	31098.0
52	26650.5
53	22203.0
54	18699.0
55	15195.0
56	12980.5
57	10766.0
58	9884.0
59	9002.0
60	8323.5
61	7645.0
62	6500.5
63	5356.0
64	4565.0
65	3774.0
66	3236.0
67	2698.0
68	2234.0
69	1770.0
70	1427.5
71	1085.0
72	980.0
73	875.0
74	741.5
75	454.0
76	300.0
77	238.0
78	176.0
79	130.5
80	85.0
81	66.0
82	47.0
83	34.5
84	22.0
85	18.5
86	15.0
87	10.0
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	3.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	299147.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.12610731283508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.73339402187351	37.89131769800412
2	8.995936636339492	8.658788238798781
3	3.519319092336961	5.081133849177538
4	1.962940556589143	3.7787475150050134
5	1.206224468286663	2.9025444102065685
6	0.8817761860522543	2.5461873213511934
7	0.6444507060334539	2.1710432685478835
8	0.4785346732280158	1.8424008829347165
9	0.3664730918884954	1.587323101274298
>10	3.1316855142954534	30.097897625317067
>50	0.06466359593089957	1.975287983634006
>100	0.013906149662559049	1.2598609619485064
>500	6.953074831279524E-4	0.20746714380028525
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	620	0.20725596445894495	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	405	0.13538494452560113	No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	358	0.11967360528435853	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3428381364345957E-4	0.0	0.0	0.20324455869522343	0.0
2	3.3428381364345957E-4	0.0	0.0	0.9517060174429294	0.0
3	3.3428381364345957E-4	0.0	0.0	1.349169471865003	0.0
4	3.3428381364345957E-4	0.0	0.0	1.8626294096213567	0.0
5	3.3428381364345957E-4	0.0	0.0	3.2642814402283826	0.0
6	3.3428381364345957E-4	0.0	0.0	4.194927577411774	0.0
7	3.3428381364345957E-4	0.0	0.0	5.150310716804782	0.0
8	3.3428381364345957E-4	0.0	0.0	7.0657569689818045	0.0
9	3.3428381364345957E-4	0.0	0.0	7.774772937719582	0.0
10	3.3428381364345957E-4	0.0	0.0	9.574556990375969	0.0
11	3.3428381364345957E-4	0.0	0.0	11.645445215897201	0.0
12	3.3428381364345957E-4	0.0	0.0	13.102588359569042	0.0
13	3.3428381364345957E-4	0.0	0.0	13.750096106596423	0.0
14	3.3428381364345957E-4	0.0	0.0	13.981420505637697	0.0
15	3.3428381364345957E-4	0.0	0.0	14.409638070914967	0.0
16	3.3428381364345957E-4	0.0	0.0	15.434886527359458	0.0
17	3.3428381364345957E-4	0.0	0.0	16.753636172182908	0.0
18	3.3428381364345957E-4	0.0	0.0	18.240530575269016	0.0
19	3.3428381364345957E-4	0.0	0.0	19.076574393191308	0.0
20	3.3428381364345957E-4	0.0	0.0	19.837404353043823	0.0
21	3.3428381364345957E-4	0.0	0.0	20.87301560771126	0.0
22	3.3428381364345957E-4	0.0	0.0	21.898932631783037	0.0
23	3.3428381364345957E-4	0.0	0.0	23.123748524972672	0.0
24	3.3428381364345957E-4	0.0	0.0	23.97282941162706	0.0
25	3.3428381364345957E-4	0.0	0.0	24.724967992324842	0.0
26	3.3428381364345957E-4	0.0	0.0	25.37849284799781	0.0
27	3.3428381364345957E-4	0.0	0.0	26.032686271298058	0.0
28	3.3428381364345957E-4	0.0	0.0	26.706936723416916	0.0
29	3.3428381364345957E-4	0.0	0.0	27.420632665545703	0.0
30	3.3428381364345957E-4	0.0	0.0	28.168759840479765	0.0
31	3.3428381364345957E-4	0.0	0.0	28.918892718295687	0.0
32	3.3428381364345957E-4	0.0	0.0	29.60684880677393	0.0
33	3.3428381364345957E-4	0.0	0.0	30.29213062474302	0.0
34	3.3428381364345957E-4	0.0	0.0	30.935292682193037	0.0
35	3.3428381364345957E-4	0.0	0.0	31.628263027875928	0.0
36	3.3428381364345957E-4	0.0	0.0	32.331261887968125	0.0
37	3.3428381364345957E-4	0.0	0.0	33.02222653076915	0.0
38	3.3428381364345957E-4	0.0	0.0	33.65469150618258	0.0
39	3.3428381364345957E-4	0.0	0.0	34.26475946608189	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACT	20	7.024909E-4	45.000004	27
GTTGACG	20	7.024909E-4	45.000004	1
CGGGTAT	20	7.024909E-4	45.000004	6
TCGTTGT	20	7.024909E-4	45.000004	20
GTATTAG	45	3.8198777E-10	45.000004	1
CATGCGG	20	7.024909E-4	45.000004	2
CGTGCGG	20	7.024909E-4	45.000004	2
CGCGCAT	20	7.024909E-4	45.000004	45
CTAACGG	20	7.024909E-4	45.000004	2
CAGATCG	20	7.024909E-4	45.000004	14
TATAGCG	20	7.024909E-4	45.000004	1
CGTAAAG	20	7.024909E-4	45.000004	2
GCCAGTA	20	7.024909E-4	45.000004	25
ATCGGGT	20	7.024909E-4	45.000004	4
ATTGCCC	20	7.024909E-4	45.000004	13
CTCTCGC	20	7.024909E-4	45.000004	11
CCACGGA	20	7.024909E-4	45.000004	13
GTCCTGC	35	1.2078999E-7	45.0	14
CGGTTTT	55	1.8189894E-12	45.0	1
AATGGTC	35	1.2078999E-7	45.0	10
>>END_MODULE