##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936296.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556515
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.901240757212296	31.0	31.0	34.0	30.0	34.0
2	32.03626676729289	33.0	31.0	34.0	30.0	34.0
3	31.981420087508873	33.0	31.0	34.0	30.0	34.0
4	35.70882545843329	37.0	35.0	37.0	35.0	37.0
5	31.18530318140571	37.0	35.0	37.0	0.0	37.0
6	33.39482673422999	37.0	35.0	37.0	17.0	37.0
7	29.518697609228862	35.0	32.0	37.0	0.0	37.0
8	32.54274008786825	35.0	33.0	37.0	17.0	37.0
9	36.417972561386485	39.0	35.0	39.0	32.0	39.0
10	36.795178925994804	38.0	35.0	39.0	33.0	39.0
11	36.925495269669284	39.0	37.0	39.0	33.0	39.0
12	36.96307556849321	39.0	37.0	39.0	33.0	39.0
13	36.77830965921853	39.0	37.0	39.0	33.0	39.0
14	38.04756924790886	40.0	37.0	41.0	33.0	41.0
15	38.13996208547838	40.0	37.0	41.0	33.0	41.0
16	38.17207802125729	40.0	37.0	41.0	33.0	41.0
17	38.120607710483995	40.0	37.0	41.0	33.0	41.0
18	38.034153616703954	40.0	37.0	41.0	33.0	41.0
19	37.94892141272023	40.0	37.0	41.0	33.0	41.0
20	37.89073430186069	40.0	36.0	41.0	33.0	41.0
21	37.81247944799332	40.0	36.0	41.0	33.0	41.0
22	37.806732972157086	40.0	36.0	41.0	33.0	41.0
23	37.75575141730232	40.0	36.0	41.0	33.0	41.0
24	37.68594557199716	40.0	36.0	41.0	33.0	41.0
25	37.559718965346846	39.0	36.0	41.0	33.0	41.0
26	37.37545259337125	39.0	36.0	41.0	32.0	41.0
27	37.324738776133614	39.0	36.0	41.0	32.0	41.0
28	37.226753995849165	39.0	36.0	41.0	32.0	41.0
29	37.1864028822224	39.0	36.0	41.0	32.0	41.0
30	37.05177218942886	39.0	35.0	41.0	31.0	41.0
31	36.92736404229895	39.0	35.0	41.0	31.0	41.0
32	36.830712559409896	39.0	35.0	41.0	31.0	41.0
33	36.67791703727662	39.0	35.0	41.0	30.0	41.0
34	36.54278141649372	39.0	35.0	41.0	30.0	41.0
35	36.46263263344205	39.0	35.0	41.0	30.0	41.0
36	36.35021877218045	39.0	35.0	40.0	30.0	41.0
37	36.26941052801811	39.0	35.0	40.0	30.0	41.0
38	36.158565357627374	39.0	35.0	40.0	29.0	41.0
39	36.104182277207265	39.0	35.0	40.0	29.0	41.0
40	35.9521055137777	39.0	35.0	40.0	29.0	41.0
41	35.86527586857497	38.0	35.0	40.0	28.0	41.0
42	35.820633765487	38.0	35.0	40.0	28.0	41.0
43	35.6540129196877	38.0	35.0	40.0	27.0	41.0
44	35.38262760213112	38.0	34.0	40.0	26.0	41.0
45	35.279282678813686	38.0	34.0	40.0	26.0	41.0
46	35.153131541827264	38.0	34.0	40.0	25.0	41.0
47	34.99874756295877	38.0	34.0	40.0	25.0	41.0
48	34.932806842582856	38.0	34.0	40.0	24.0	41.0
49	34.76869087086602	38.0	34.0	40.0	24.0	41.0
50	34.59620675094112	37.0	33.0	40.0	24.0	41.0
51	32.5579508189357	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	10.0
9	18.0
10	36.0
11	30.0
12	20.0
13	23.0
14	24.0
15	34.0
16	96.0
17	147.0
18	258.0
19	480.0
20	800.0
21	1305.0
22	1874.0
23	2519.0
24	3390.0
25	4612.0
26	5933.0
27	6819.0
28	8310.0
29	9843.0
30	12330.0
31	15611.0
32	19838.0
33	27610.0
34	39430.0
35	47864.0
36	56221.0
37	86697.0
38	105503.0
39	98817.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13738174173203	20.34716045389612	24.67588474704186	11.839573057329991
2	30.961249921385768	26.233434858000233	25.29599381867515	17.509321401938852
3	31.836877712191047	25.093124174550553	26.51842268402469	16.551575429233715
4	27.865915563821282	26.520039891108055	26.65929938995355	18.954745155117113
5	21.84289731633469	39.601627988463925	20.958464731408856	17.59700996379253
6	25.379908897334303	35.93056790922077	24.249481146060752	14.440042047384166
7	69.29444848746216	19.6257064050385	6.784004025048741	4.2958410824506075
8	83.07377159645293	5.839375398686468	7.068812161397267	4.018040843463338
9	76.87591529428677	7.90454884414616	8.841810193795316	6.377725667771758
10	41.50238538044796	27.33511226112504	16.196868008948545	14.965634349478451
11	30.913632157264402	27.28533822089252	23.118154946407554	18.682874675435524
12	29.71510201881351	23.515089440536194	27.13853175565798	19.63127678499232
13	24.2830831154596	24.739494892320963	29.050070528197804	21.927351464021637
14	19.776466043143493	28.913146995139392	28.32735865160867	22.98302831010844
15	19.82480256596857	26.16407464309138	33.15346396772774	20.857658823212315
16	22.546202707923417	25.427886040807522	30.869069117633845	21.156842133635212
17	21.934359361382892	25.422135971177777	28.45206328670386	24.19144138073547
18	22.94655130589472	26.624259903147262	28.803895672174157	21.625293118783862
19	25.03975634079944	27.664663126780052	25.918079476743667	21.377501055676845
20	26.67511208143536	25.90442306137301	27.455684033673844	19.964780823517785
21	25.663818585303183	27.32433088056926	26.274404104112197	20.737446430015364
22	23.8106789574405	24.857910388758615	26.530641581987908	24.80076907181298
23	23.93089135063745	26.4803284727276	27.543192905851594	22.045587270783358
24	24.048228709019522	24.48990593245465	28.06159762090869	23.400267737617135
25	22.670727653342677	26.896489762180714	26.79029316370628	23.642489420770328
26	22.247199087176448	28.23751381364384	26.26613837901943	23.249148720160285
27	23.05957611205448	27.75378920604117	26.637916318517917	22.548718363386435
28	20.97355866418695	28.1967242572078	27.833032353126153	22.996684725479096
29	22.534343189312057	27.1834541746404	27.80266479789404	22.47953783815351
30	22.806213668993646	26.769988230326224	26.76729288518728	23.656505215492842
31	22.885816195430493	27.3027681194577	25.23328212177569	24.57813356333612
32	24.51110931421435	28.554127022631913	25.000044922418986	21.934718740734752
33	23.149600639695247	28.37138262221144	24.100159025363197	24.378857712730113
34	21.295023494425127	27.989002991833107	26.574845242266605	24.14112827147516
35	21.85493652462198	26.493984888098254	26.535852582589868	25.1152260046899
36	21.246507281924117	29.49731813158675	25.385119897936264	23.87105468855287
37	22.232284844074286	28.425469214666272	25.94179851396638	23.400447427293063
38	22.278644780464138	28.294295751237613	25.124030798810455	24.303028669487794
39	22.524460257135924	26.99711598070133	25.93658751336442	24.541836248798326
40	22.539554189914018	25.373260379324908	29.197056683108276	22.890128747652806
41	19.5748542267504	26.83252023754975	28.40857838512888	25.184047150570965
42	21.656918501747484	26.04997169887604	27.72054661599418	24.572563183382297
43	22.27325409018625	25.409737383538626	27.170336828297533	25.14667169797759
44	21.864460077446253	26.503508440922523	26.681940289120686	24.950091192510534
45	21.230514900766376	26.52561027106188	26.481945679810963	25.761929148360778
46	21.51029172618887	27.839321491783693	25.694365830211225	24.956020951816214
47	20.730618222330037	26.65426807902752	28.184864738596442	24.430248960046
48	21.400501334195845	24.98387285158531	28.12394993845629	25.49167587576256
49	20.698813149690483	25.641896444839762	28.54029091758533	25.118999487884423
50	19.453204316146017	26.378264736799544	28.12628590424337	26.042245042811064
51	19.83809960198737	25.287728093582384	27.123437822879886	27.75073448155036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	286.0
1	590.0
2	894.0
3	1562.5
4	2231.0
5	1738.0
6	1245.0
7	1080.0
8	915.0
9	911.5
10	908.0
11	938.0
12	968.0
13	999.0
14	1030.0
15	1074.0
16	1118.0
17	1185.5
18	1253.0
19	1369.0
20	1485.0
21	1754.0
22	2023.0
23	2272.5
24	2522.0
25	3104.0
26	4463.0
27	5240.0
28	6071.5
29	6903.0
30	8830.0
31	10757.0
32	12135.5
33	13514.0
34	15375.5
35	17237.0
36	18515.5
37	19794.0
38	21180.5
39	22567.0
40	24574.5
41	26582.0
42	28532.0
43	30482.0
44	33926.5
45	37371.0
46	41658.5
47	45946.0
48	46480.5
49	47015.0
50	44934.0
51	42853.0
52	38508.5
53	34164.0
54	31501.5
55	28839.0
56	28561.0
57	28283.0
58	28098.0
59	27913.0
60	27497.5
61	27082.0
62	23847.0
63	20612.0
64	17391.5
65	14171.0
66	11860.0
67	9549.0
68	8122.0
69	6695.0
70	5734.0
71	4773.0
72	3872.5
73	2972.0
74	2507.0
75	1559.5
76	1077.0
77	849.0
78	621.0
79	496.5
80	372.0
81	315.5
82	259.0
83	186.5
84	114.0
85	93.0
86	72.0
87	56.5
88	41.0
89	29.0
90	17.0
91	12.5
92	8.0
93	7.0
94	6.0
95	5.5
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	556515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.79222447264551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73781645228618	26.03448254110315
2	8.702810412021655	6.2298588761991125
3	4.04678863395967	4.345307015401049
4	2.6529941266540598	3.798262452232489
5	1.9451847208739004	3.481124408513946
6	1.3745813064165104	2.951959360509724
7	1.0324819216263672	2.586837729195953
8	0.7877735640947163	2.255693459175527
9	0.6262415274025265	2.0173119590595245
>10	6.000841427662003	42.55160902789933
>50	0.06961304828153292	1.569533713852178
>100	0.02038667842518909	1.3543657514863694
>500	0.0014917081774528601	0.4370988548818033
>1k	9.944721183019068E-4	0.38655485048993354
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG	1133	0.20358840282831547	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC	1039	0.18669757329092657	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC	897	0.16118163930891352	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	782	0.14051732657700153	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	777	0.13961887819735316	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.593793518593389E-4	0.0	0.0	0.0812197335202106	0.0
2	3.593793518593389E-4	0.0	0.0	0.3737545259337125	0.0
3	3.593793518593389E-4	0.0	0.0	0.5040295409827228	0.0
4	3.593793518593389E-4	0.0	0.0	0.724688463024357	0.0
5	3.593793518593389E-4	0.0	0.0	1.4978931385497247	0.0
6	3.593793518593389E-4	0.0	0.0	1.949992363188773	0.0
7	3.593793518593389E-4	0.0	0.0	2.3918492762998302	0.0
8	3.593793518593389E-4	0.0	0.0	3.2202186823356063	0.0
9	3.593793518593389E-4	0.0	0.0	3.5567774453518775	0.0
10	3.593793518593389E-4	0.0	0.0	4.543094076529833	0.0
11	3.593793518593389E-4	0.0	0.0	5.342892824092791	0.0
12	3.593793518593389E-4	0.0	0.0	6.193543749943847	0.0
13	3.593793518593389E-4	0.0	0.0	6.487156680412927	0.0
14	3.593793518593389E-4	0.0	0.0	6.611681625832188	0.0
15	3.593793518593389E-4	0.0	0.0	6.837371858799853	0.0
16	3.593793518593389E-4	0.0	0.0	7.288572635059253	0.0
17	3.593793518593389E-4	0.0	0.0	7.76277368983765	0.0
18	3.593793518593389E-4	0.0	0.0	8.30184271762666	0.0
19	3.593793518593389E-4	0.0	0.0	8.624924754948205	0.0
20	3.593793518593389E-4	0.0	0.0	8.952678723843922	0.0
21	3.593793518593389E-4	0.0	0.0	9.382316738991761	0.0
22	3.593793518593389E-4	0.0	0.0	9.832439377195584	0.0
23	3.593793518593389E-4	0.0	0.0	10.289390223084732	0.0
24	3.593793518593389E-4	0.0	0.0	10.646613298832916	0.0
25	3.593793518593389E-4	0.0	0.0	10.97095316388597	0.0
26	3.593793518593389E-4	0.0	0.0	11.271394302040376	0.0
27	3.593793518593389E-4	0.0	0.0	11.56878071570398	0.0
28	3.593793518593389E-4	0.0	0.0	11.885034545340197	0.0
29	3.593793518593389E-4	0.0	0.0	12.218179204513804	0.0
30	3.593793518593389E-4	0.0	0.0	12.587800867901136	0.0
31	3.593793518593389E-4	0.0	0.0	12.943227046890021	0.0
32	3.593793518593389E-4	0.0	0.0	13.29721570847147	0.0
33	3.593793518593389E-4	0.0	0.0	13.611672641348392	0.0
34	3.593793518593389E-4	0.0	0.0	13.941403196679335	0.0
35	3.593793518593389E-4	0.0	0.0	14.289821478306964	0.0
36	5.390690277890084E-4	0.0	0.0	14.658185313962786	0.0
37	5.390690277890084E-4	0.0	0.0	15.01540838971097	0.0
38	5.390690277890084E-4	0.0	0.0	15.351428083699451	0.0
39	5.390690277890084E-4	0.0	0.0	15.688705605419441	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACGGT	25	3.8882026E-5	45.000004	16
GCTACGC	20	7.0301467E-4	45.0	42
CAGTACG	20	7.0301467E-4	45.0	1
TAATGCG	20	7.0301467E-4	45.0	1
ACGGGTA	65	0.0	41.538464	5
AACACGT	60	3.6379788E-12	41.249996	41
ATAACGG	50	1.0786607E-9	40.500004	2
GTATGCG	45	1.9252184E-8	40.0	1
ATTGGGC	130	0.0	39.807693	4
TAACGGG	200	0.0	39.375004	3
TACGGGT	40	3.4539335E-7	39.375	4
GACCGCC	35	6.2424824E-6	38.571426	9
TCTACGG	35	6.2424824E-6	38.571426	2
TGCACGG	155	0.0	37.741936	2
TATGCGG	90	0.0	37.5	2
ACGCGAG	30	1.13916554E-4	37.499996	2
GCGACAA	30	1.13916554E-4	37.499996	18
GTTTCGC	30	1.13916554E-4	37.499996	16
GATATAC	30	1.13916554E-4	37.499996	9
TTGCGAG	60	1.546141E-10	37.499996	1
>>END_MODULE