##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936295.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336812
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.878059570324098	31.0	31.0	34.0	30.0	34.0
2	32.01426314976901	33.0	31.0	34.0	30.0	34.0
3	31.96039927318504	33.0	31.0	34.0	30.0	34.0
4	35.68396612947282	37.0	35.0	37.0	33.0	37.0
5	31.11852903103215	37.0	35.0	37.0	0.0	37.0
6	33.35488937448785	37.0	35.0	37.0	17.0	37.0
7	29.53741553151313	35.0	32.0	37.0	0.0	37.0
8	32.5280779782193	35.0	33.0	37.0	17.0	37.0
9	36.33005356103702	39.0	35.0	39.0	32.0	39.0
10	36.78448511335701	38.0	35.0	39.0	33.0	39.0
11	36.90616723869696	39.0	37.0	39.0	33.0	39.0
12	36.823533603315795	39.0	35.0	39.0	33.0	39.0
13	36.50698015510136	39.0	35.0	39.0	32.0	39.0
14	37.751181668111585	40.0	36.0	41.0	33.0	41.0
15	37.898913934182865	40.0	36.0	41.0	33.0	41.0
16	37.95729665213828	40.0	36.0	41.0	33.0	41.0
17	37.91959015712029	40.0	36.0	41.0	33.0	41.0
18	37.82079320214244	40.0	36.0	41.0	33.0	41.0
19	37.76617222664276	40.0	36.0	41.0	33.0	41.0
20	37.71408679025688	39.0	36.0	41.0	33.0	41.0
21	37.61037908388062	39.0	36.0	41.0	33.0	41.0
22	37.58110756148831	39.0	36.0	41.0	33.0	41.0
23	37.56242354785459	39.0	35.0	41.0	33.0	41.0
24	37.481179411659916	39.0	35.0	41.0	33.0	41.0
25	37.37717480374808	39.0	35.0	41.0	32.0	41.0
26	37.17220882866406	39.0	35.0	41.0	32.0	41.0
27	37.14423179696686	39.0	35.0	41.0	32.0	41.0
28	36.990151776064984	39.0	35.0	41.0	31.0	41.0
29	36.98377433108084	39.0	35.0	41.0	31.0	41.0
30	36.86567283825992	39.0	35.0	41.0	31.0	41.0
31	36.73421077633814	39.0	35.0	41.0	31.0	41.0
32	36.627795327957436	39.0	35.0	40.0	31.0	41.0
33	36.47966521382848	39.0	35.0	40.0	30.0	41.0
34	36.36184280845101	39.0	35.0	40.0	30.0	41.0
35	36.2972934456017	39.0	35.0	40.0	30.0	41.0
36	36.14343610085152	39.0	35.0	40.0	30.0	41.0
37	36.05155101362184	38.0	35.0	40.0	29.0	41.0
38	35.958421908958115	38.0	35.0	40.0	29.0	41.0
39	35.9416499412135	38.0	35.0	40.0	29.0	41.0
40	35.77695569041483	38.0	35.0	40.0	28.0	41.0
41	35.662532807619684	38.0	35.0	40.0	28.0	41.0
42	35.67549552866287	38.0	35.0	40.0	28.0	41.0
43	35.48567153189316	38.0	34.0	40.0	27.0	41.0
44	35.31263434794485	38.0	34.0	40.0	27.0	41.0
45	35.2355349571868	38.0	34.0	40.0	26.0	41.0
46	35.09946201441754	38.0	34.0	40.0	26.0	41.0
47	34.985445886726126	38.0	34.0	40.0	25.0	41.0
48	34.828156360224696	37.0	34.0	40.0	24.0	41.0
49	34.66185587211857	37.0	34.0	40.0	24.0	41.0
50	34.509245513817795	37.0	34.0	40.0	24.0	41.0
51	32.30207949835516	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	19.0
10	12.0
11	11.0
12	9.0
13	13.0
14	11.0
15	21.0
16	54.0
17	108.0
18	182.0
19	316.0
20	505.0
21	766.0
22	1192.0
23	1694.0
24	2283.0
25	2871.0
26	3675.0
27	4325.0
28	5027.0
29	6057.0
30	7554.0
31	9983.0
32	12712.0
33	18623.0
34	26816.0
35	31259.0
36	32933.0
37	50365.0
38	61245.0
39	56158.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.510064962055985	21.72962958564422	24.704879873638706	11.055425578661094
2	30.90804365640179	27.587496882533873	24.20667909694429	17.297780364120044
3	33.09056684441172	24.469436955927936	25.655855492084605	16.78414070757574
4	28.76055484958968	25.89040770518865	25.88714178829733	19.461895656924337
5	22.259301925109558	39.39556785387694	21.39977197962068	16.945358241392825
6	26.523995582105155	35.79652743963992	23.61584504115055	14.063631937104379
7	68.03884659691461	20.04738548507773	7.361376673040152	4.552391244967519
8	79.6643231238792	7.728346971010534	8.089082336733846	4.518247568376424
9	72.92673657708157	9.812001947674073	10.08040093583364	7.180860539410709
10	43.151669180433	27.76920062230562	15.899671033098583	13.179459164162797
11	34.16030307708752	25.486027813735852	23.395544101754094	16.958125007422538
12	32.8485920929183	22.91842333408548	26.548044606486705	17.684939966509507
13	25.528187831787463	27.22498010759712	27.250513639656543	19.996318420958872
14	18.791788891132143	31.12715698965595	27.919135897770865	22.16191822144104
15	18.986556298469175	26.828913459140413	35.622543139793116	18.561987102597293
16	21.619776017481563	25.752942294217547	32.21322280678835	20.414058881512535
17	21.197878935429852	25.754723703430994	27.730306521145327	25.31709083999382
18	22.237925014548175	28.1596261415864	28.393287650083725	21.209161193781696
19	26.564967994014467	27.797109366649643	24.663610560193817	20.974312079142074
20	27.754652447062455	26.635630559481253	26.747265536857356	18.862451456598933
21	24.803748084985095	27.701210170659003	26.69679227580965	20.798249468546253
22	23.209683740484305	26.96548816550479	25.873781219196466	23.951046874814434
23	24.528817263042885	26.757360189066898	26.848508960488342	21.865313587401875
24	23.613469828865956	25.954241535337218	27.957139294324428	22.475149341472395
25	22.089474246760805	28.57024096528627	25.9180195479971	23.422265239955824
26	21.021816324834035	30.02179257271119	26.141586404284883	22.8148046981699
27	22.340652945857038	29.71153046803558	26.246986449413917	21.700830136693465
28	19.375794211607662	28.985606213555336	29.994774532973885	21.643825041863117
29	21.99476265691246	26.700058192700972	28.423868508247924	22.88131064213864
30	21.862047670510552	26.77547118273696	27.32236381126563	24.040117335486862
31	24.285951806942744	27.515052907853637	25.635666187665525	22.563329097538094
32	25.747894968112778	27.85441136301557	24.725366079593364	21.672327589278293
33	22.892889802026055	29.110007956961155	24.60808997304134	23.389012267971452
34	21.526548935311094	28.72462976378514	26.929859981235822	22.818961319667945
35	22.89556191584623	25.23781812999537	26.528746006674346	25.337873947484056
36	22.15687089533627	28.600524921914893	27.373133973848912	21.869470208899923
37	21.559801907295466	30.369761172404786	26.92332814745318	21.147108772846572
38	22.352232105744452	26.83514839138748	25.601522511074425	25.211096991793642
39	24.365521418476778	26.04954692825671	27.006460577414103	22.578471075852406
40	22.810054273600702	25.98749450732159	28.494827975250285	22.707623243827417
41	19.067313516145507	27.469626972910703	28.66465565359904	24.79840385734475
42	22.135197083239312	26.410876097051172	27.636782537439288	23.817144282270228
43	23.962032231630705	25.15349809389214	27.07682624134532	23.807643433131837
44	22.180326116646675	26.763001318242818	27.055152429248363	24.001520135862144
45	21.178580335617493	27.60946759616641	24.985154923221263	26.226797144994833
46	22.059487191667756	27.80868852653706	25.706625654667885	24.4251986271273
47	22.400330154507557	25.681092122608458	29.037860883816492	22.880716839067492
48	21.58117881785685	25.306996187784282	28.803308670712447	24.308516323646426
49	20.304502214885456	25.487809222949302	30.105815707278836	24.101872854886405
50	19.985926867213756	25.618148997066616	28.863876583969695	25.532047551749937
51	20.083310570882272	25.195658111943754	27.452406683847368	27.268624633326606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	193.0
1	414.5
2	636.0
3	947.5
4	1259.0
5	1033.5
6	808.0
7	684.5
8	561.0
9	572.0
10	583.0
11	641.0
12	699.0
13	712.0
14	725.0
15	773.5
16	822.0
17	782.5
18	743.0
19	795.5
20	848.0
21	890.5
22	933.0
23	1127.0
24	1321.0
25	1981.5
26	2962.0
27	3282.0
28	4112.5
29	4943.0
30	5987.0
31	7031.0
32	7875.5
33	8720.0
34	9205.5
35	9691.0
36	10145.0
37	10599.0
38	11682.5
39	12766.0
40	15128.5
41	17491.0
42	19728.5
43	21966.0
44	24153.0
45	26340.0
46	28991.0
47	31642.0
48	29971.0
49	28300.0
50	26206.0
51	24112.0
52	21998.0
53	19884.0
54	18202.0
55	16520.0
56	15860.0
57	15200.0
58	15240.0
59	15280.0
60	15033.0
61	14786.0
62	13744.0
63	12702.0
64	10212.5
65	7723.0
66	6295.5
67	4868.0
68	4127.5
69	3387.0
70	2934.5
71	2482.0
72	2087.0
73	1692.0
74	1411.5
75	873.0
76	615.0
77	527.5
78	440.0
79	322.0
80	204.0
81	160.0
82	116.0
83	89.0
84	62.0
85	51.0
86	40.0
87	27.5
88	15.0
89	10.5
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	336812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.8825698690944
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.03526534058688	26.565755813605513
2	8.711038409348925	6.251488887116555
3	3.8256525084016	4.118227302827901
4	2.3572476078172526	3.3833640794503275
5	1.7603321294622183	3.1582620314119882
6	1.3090869997954775	2.8184043440930626
7	1.0114581859946108	2.5405603320033365
8	0.7139529566652295	2.0494773480629296
9	0.6048734286298463	1.9533971758292228
>10	5.523179639305748	41.31007256503384
>50	0.11072801337963713	2.4918522765535354
>100	0.03387946678033674	2.437591333358712
>500	0.003305313832227974	0.9215465106530686
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCG	940	0.2790874434402575	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTC	820	0.2434592591712884	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGC	732	0.21733192404071114	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	616	0.18289134591404108	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	474	0.1407313278624277	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	416	0.12351103879909267	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCT	395	0.11727610655202307	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	394	0.11697920501644835	No Hit
GCTGTCTCTTATACACATCTGACGCTCTGCACTTCGTATGCCGTCTTCTGC	382	0.11341638658955144	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	380	0.11282258351840196	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	348	0.10332173438001022	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	346	0.10272793130886072	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9690153557474197E-4	0.0	0.0	0.10094652209541227	0.0
2	2.9690153557474197E-4	0.0	0.0	0.5210621949336722	0.0
3	2.9690153557474197E-4	0.0	0.0	0.7090008669524839	0.0
4	2.9690153557474197E-4	0.0	0.0	1.0842844079189577	0.0
5	2.9690153557474197E-4	0.0	0.0	2.083358075127965	0.0
6	2.9690153557474197E-4	0.0	0.0	2.6139211192000285	0.0
7	2.9690153557474197E-4	0.0	0.0	3.156954027766232	0.0
8	2.9690153557474197E-4	0.0	0.0	4.014999465577236	0.0
9	2.9690153557474197E-4	0.0	0.0	4.357326936094913	0.0
10	2.9690153557474197E-4	0.0	0.0	5.45408120850801	0.0
11	2.9690153557474197E-4	0.0	0.0	6.370913150362814	0.0
12	2.9690153557474197E-4	0.0	0.0	7.463510801277864	0.0
13	2.9690153557474197E-4	0.0	0.0	7.941225372017624	0.0
14	2.9690153557474197E-4	0.0	0.0	8.117881785684595	0.0
15	2.9690153557474197E-4	0.0	0.0	8.41181430590359	0.0
16	2.9690153557474197E-4	0.0	0.0	8.959894540574563	0.0
17	2.9690153557474197E-4	0.0	0.0	9.59763903898911	0.0
18	2.9690153557474197E-4	0.0	0.0	10.332173438001021	0.0
19	2.9690153557474197E-4	0.0	0.0	10.727943184922152	0.0
20	2.9690153557474197E-4	0.0	0.0	11.13915181169317	0.0
21	2.9690153557474197E-4	0.0	0.0	11.622804413144424	0.0
22	2.9690153557474197E-4	0.0	0.0	12.136740971224302	0.0
23	5.938030711494839E-4	0.0	0.0	12.610002018930443	0.0
24	8.90704606724226E-4	0.0	0.0	13.006662470458297	0.0
25	8.90704606724226E-4	0.0	0.0	13.362053608541263	0.0
26	8.90704606724226E-4	0.0	0.0	13.685676282317733	0.0
27	8.90704606724226E-4	0.0	0.0	14.020284312910466	0.0
28	8.90704606724226E-4	0.0	0.0	14.37122192795981	0.0
29	8.90704606724226E-4	0.0	0.0	14.732551096754273	0.0
30	8.90704606724226E-4	0.0	0.0	15.13247746517345	0.0
31	8.90704606724226E-4	0.0	0.0	15.543092288873318	0.0
32	8.90704606724226E-4	0.0	0.0	15.939752740401174	0.0
33	8.90704606724226E-4	0.0	0.0	16.309098250656152	0.0
34	8.90704606724226E-4	0.0	0.0	16.676959253233257	0.0
35	8.90704606724226E-4	0.0	0.0	17.041554338919042	0.0
36	8.90704606724226E-4	0.0	0.0	17.45840409486598	0.0
37	8.90704606724226E-4	0.0	0.0	17.848235811075615	0.0
38	8.90704606724226E-4	0.0	0.0	18.218768927472894	0.0
39	8.90704606724226E-4	0.0	0.0	18.6100851513604	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGG	35	1.2085002E-7	45.000004	2
CTTGTCA	25	3.8849146E-5	45.0	25
TTTAGCG	25	3.8849146E-5	45.0	1
CGGAATA	20	7.026178E-4	45.0	15
CGGCGAA	20	7.026178E-4	45.0	12
ATTAGCG	25	3.8849146E-5	45.0	1
AGTCACG	20	7.026178E-4	45.0	1
TGCTTCG	20	7.026178E-4	45.0	23
CTATCGA	25	3.8849146E-5	45.0	38
GCGAAGT	20	7.026178E-4	45.0	14
GTTAGCG	25	3.8849146E-5	45.0	1
ATTCGAA	20	7.026178E-4	45.0	8
CGATTCA	20	7.026178E-4	45.0	10
GAATACG	20	7.026178E-4	45.0	1
AGGCGAT	40	6.7902874E-9	45.0	7
TCGATGT	20	7.026178E-4	45.0	21
GGCCCGT	25	3.8849146E-5	45.0	8
TGTTGCG	20	7.026178E-4	45.0	1
GTTACGG	25	3.8849146E-5	45.0	2
CTAGTAG	20	7.026178E-4	45.0	1
>>END_MODULE