FastQCFastQC Report
Sat 14 Jan 2017
SRR2936294.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936294.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743949
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69500.9342038231115304No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19590.26332450208280406No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG18710.2514957342506005No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC16010.21520292385633963No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC15770.21197689626573862No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13720.18442124392935538No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.12258904844283681No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG351.2111559E-745.0000041
GTCAACG207.0316816E-445.01
GTATACG207.0316816E-445.01
CGTTTTA3350.042.3134351
CGTTTTT27150.041.850831
TACGGGT603.6379788E-1241.2500044
CGAATAT1100.040.9090914
GCGATAT556.002665E-1140.909099
CGTTATT5600.040.1785741
ACACGAC1250.039.626
TACGGCT4100.039.5121967
TAGCACG403.4560253E-739.3751
CGGTCTA403.4560253E-739.37531
CGGTTTT6150.038.4146351
TACGAAT1200.037.50000412
TAGTACG301.13953465E-437.5000041
ACGGCTG5150.037.135928
GACACGA1350.036.66666425
ACGACCA1350.036.66666428
CTATCTC1750.036.05
Produced by FastQC (version 0.11.5)