##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936294.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 743949 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79761247074732 31.0 31.0 34.0 30.0 34.0 2 31.905341629600954 33.0 31.0 34.0 30.0 34.0 3 31.551920897803477 33.0 31.0 34.0 30.0 34.0 4 35.454204522084176 37.0 35.0 37.0 33.0 37.0 5 31.075774011390564 37.0 35.0 37.0 0.0 37.0 6 33.2958415160179 37.0 35.0 37.0 17.0 37.0 7 29.48421867628023 35.0 32.0 37.0 0.0 37.0 8 32.47085754534249 35.0 33.0 37.0 17.0 37.0 9 36.35683763268719 39.0 35.0 39.0 32.0 39.0 10 36.805490698959204 38.0 35.0 39.0 33.0 39.0 11 36.91570793159209 39.0 37.0 39.0 33.0 39.0 12 36.8957845228638 39.0 37.0 39.0 33.0 39.0 13 36.71165899813025 39.0 35.0 39.0 33.0 39.0 14 37.893093478181974 40.0 37.0 41.0 33.0 41.0 15 37.98341418564982 40.0 37.0 41.0 33.0 41.0 16 38.05573500334028 40.0 37.0 41.0 33.0 41.0 17 37.99607634394293 40.0 37.0 41.0 33.0 41.0 18 37.83436767842957 40.0 37.0 41.0 33.0 41.0 19 37.69863660008952 39.0 36.0 41.0 33.0 41.0 20 37.575858022525736 39.0 35.0 41.0 33.0 41.0 21 37.489783573874014 39.0 35.0 41.0 33.0 41.0 22 37.4829820323705 39.0 35.0 41.0 33.0 41.0 23 37.44779413642602 39.0 35.0 41.0 33.0 41.0 24 37.357036571055275 39.0 35.0 41.0 33.0 41.0 25 37.21421764126304 39.0 35.0 41.0 32.0 41.0 26 37.046513941143814 39.0 35.0 41.0 32.0 41.0 27 36.99928624139558 39.0 35.0 41.0 32.0 41.0 28 36.87576298912963 39.0 35.0 40.0 31.0 41.0 29 36.80087747950464 39.0 35.0 40.0 31.0 41.0 30 36.65280415727422 39.0 35.0 40.0 31.0 41.0 31 36.456941268823535 39.0 35.0 40.0 30.0 41.0 32 36.264713038124924 38.0 35.0 40.0 30.0 41.0 33 35.993088235887136 38.0 35.0 40.0 29.0 41.0 34 35.69714052979438 38.0 35.0 40.0 27.0 41.0 35 35.38714481772272 38.0 34.0 40.0 25.0 41.0 36 35.194009266764255 38.0 34.0 40.0 25.0 41.0 37 35.075069662033286 38.0 34.0 40.0 24.0 41.0 38 34.80868849880839 38.0 34.0 40.0 23.0 41.0 39 34.69196409968963 38.0 34.0 40.0 23.0 41.0 40 34.39910397083671 38.0 33.0 40.0 21.0 41.0 41 34.254801068352805 38.0 33.0 40.0 21.0 41.0 42 34.122816214552344 38.0 33.0 40.0 20.0 41.0 43 33.97583302081191 38.0 33.0 40.0 18.0 41.0 44 33.771993779143465 37.0 33.0 40.0 18.0 41.0 45 33.62658192967529 37.0 33.0 40.0 18.0 41.0 46 33.42955229457933 37.0 32.0 40.0 17.0 41.0 47 33.219478754592046 37.0 32.0 40.0 15.0 41.0 48 33.075339841844 36.0 32.0 40.0 15.0 41.0 49 32.925707272944784 36.0 31.0 40.0 15.0 41.0 50 32.81289174392331 36.0 31.0 40.0 15.0 41.0 51 30.885142664349303 35.0 27.0 39.0 10.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 13.0 9 48.0 10 48.0 11 48.0 12 23.0 13 31.0 14 46.0 15 56.0 16 124.0 17 278.0 18 482.0 19 898.0 20 1409.0 21 2147.0 22 3335.0 23 5321.0 24 7686.0 25 11166.0 26 14267.0 27 16258.0 28 17273.0 29 17994.0 30 20240.0 31 23722.0 32 29211.0 33 39612.0 34 54070.0 35 61606.0 36 71914.0 37 106886.0 38 125475.0 39 112252.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.44934666220399 19.112331624882888 23.405233423258853 16.033088289654263 2 34.94634712863382 24.732743776791153 24.02785674824484 16.293052346330192 3 31.37553783928737 24.54980112884082 28.352749986894267 15.721911044977546 4 26.551820084441275 24.24413501463138 31.084254431419357 18.11979046950799 5 22.26456383434886 37.14837979485153 24.007962911436135 16.579093459363477 6 24.584346507623504 33.81696863629093 27.852446874718563 13.746237981367 7 65.6432094135485 18.997942063232827 11.342444172920455 4.016404350298206 8 78.96428384203756 5.677539723825155 11.77325327408196 3.584923160055326 9 73.22881003939786 6.4441245300417105 13.27013007612081 7.056935354439618 10 41.12849133475547 24.703037439394368 20.262410460932134 13.906060764918024 11 30.305437603921774 24.65034565541455 26.91945281195351 18.124763928710166 12 27.788732829804193 22.20716742679942 30.91488798291281 19.089211760483582 13 24.70142442559907 21.767486749763762 33.84452428862731 19.68656453600986 14 20.95009200899524 24.01696890512656 33.11934016982347 21.91359891605473 15 20.311204128239975 24.00419921258043 35.83095077753986 19.853645881639736 16 25.051851672628096 24.422776292460906 30.747403383834104 19.77796865107689 17 25.348377375330838 23.54032332861527 30.954003567448847 20.15729572860505 18 24.80438847286575 23.968712909083823 31.316528417942628 19.910370200107803 19 24.64604428529375 25.800962162728897 29.49355399362053 20.05943955835682 20 25.780396236838815 26.1482977999836 29.935654191349137 18.135651771828446 21 25.647725852175352 25.817495554130726 30.446038639745467 18.08873995394846 22 24.302337929078472 22.646848103835072 31.056026690001598 21.994787277084853 23 23.193256526993114 24.737582818177053 31.590068674062334 20.4790919807675 24 23.914139275676156 23.852979169271013 30.88356863172072 21.349312923332107 25 24.03054510457034 26.02638084062214 28.561366437753126 21.381707617054392 26 23.099164055600585 24.433798553395462 29.546245777600344 22.92079161340361 27 22.726289033253625 24.52237989432071 30.476148230591075 22.27518284183459 28 21.100908798855837 25.356711279939887 30.59779635431999 22.944583566884287 29 23.070667478550277 25.283319152253714 29.020134444699842 22.625878924496167 30 23.68549457019231 24.289837072164893 30.196424754922717 21.82824360272008 31 24.258517720972808 24.99593386105768 28.77966097138379 21.965887446585718 32 26.278951917402942 24.62655370193387 28.011463151371935 21.083031229291255 33 24.469688110340897 24.89807769080945 28.316994847765102 22.31523935108455 34 23.95715297688417 24.696988637661992 29.888339120020323 21.45751926543352 35 22.434333536304234 26.1429210873326 29.557671291983723 21.86507408437944 36 23.005743673289434 27.61869429221627 28.422109580092187 20.953452454402115 37 23.892229171623324 26.713255881787596 28.164565044109207 21.229949902479873 38 23.45846287850377 27.452688289116594 27.850027354025613 21.238821478354026 39 24.969991222516597 24.67534736924171 28.267260255743338 22.087401152498355 40 24.770246347531888 24.575071678300528 29.92933655398421 20.725345420183373 41 22.209990200941192 25.23533199184353 29.58683995811541 22.96783784909987 42 22.096675981821335 25.016768622580308 30.402352849456076 22.484202546142278 43 22.522780459413212 24.30408536069005 29.54261649656092 23.63051768333582 44 22.69980872344744 24.50624975636771 29.29703514622642 23.496906373958428 45 22.752231671794707 24.18983021685626 28.433131841026736 24.624806270322296 46 23.474593016456772 25.56371471700345 27.93820544150204 23.023486825037736 47 21.562499579944326 25.236945005638827 30.836119142575637 22.364436271841214 48 21.956881452895296 25.443948442702393 29.477423855667528 23.12174624873479 49 22.659886632013755 24.520498044892864 30.011600257544536 22.808015065548847 50 21.49623159652073 24.82334138496053 30.005685873628433 23.67474114489031 51 21.573387423062602 24.341184677981957 29.581597663280686 24.503830235674755 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 251.0 1 737.5 2 1224.0 3 8174.0 4 15124.0 5 11343.5 6 7563.0 7 5969.0 8 4375.0 9 4150.0 10 3925.0 11 3842.0 12 3759.0 13 3565.5 14 3372.0 15 3261.0 16 3150.0 17 2957.0 18 2764.0 19 2724.0 20 2684.0 21 2791.0 22 2898.0 23 3082.0 24 3266.0 25 3962.0 26 5416.5 27 6175.0 28 7287.0 29 8399.0 30 10020.0 31 11641.0 32 13410.0 33 15179.0 34 17248.0 35 19317.0 36 20917.5 37 22518.0 38 25072.0 39 27626.0 40 28882.5 41 30139.0 42 33176.0 43 36213.0 44 39987.0 45 43761.0 46 50454.0 47 57147.0 48 55446.5 49 53746.0 50 53722.5 51 53699.0 52 51162.0 53 48625.0 54 45650.5 55 42676.0 56 41361.0 57 40046.0 58 38612.0 59 37178.0 60 35773.0 61 34368.0 62 31597.5 63 28827.0 64 25266.5 65 21706.0 66 18542.0 67 15378.0 68 13374.5 69 11371.0 70 9354.5 71 7338.0 72 6137.5 73 4937.0 74 4022.0 75 2398.0 76 1689.0 77 1332.0 78 975.0 79 744.0 80 513.0 81 389.0 82 265.0 83 220.0 84 175.0 85 132.0 86 89.0 87 68.5 88 48.0 89 38.5 90 29.0 91 19.5 92 10.0 93 14.5 94 19.0 95 10.5 96 2.0 97 2.5 98 3.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 743949.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.88973600603602 #Duplication Level Percentage of deduplicated Percentage of total 1 73.62094134634506 30.103408561679107 2 8.412872773317757 6.880002935066622 3 4.286142501393949 5.257777060987483 4 2.6478524288647853 4.330799471968893 5 1.8764073154401273 3.8362899884070774 6 1.3791320094964814 3.3835406271471045 7 1.0851396404954927 3.1059751400681863 8 0.8897211677442729 2.9104370934436323 9 0.7576491815327587 2.7882067516257383 >10 4.999234316376736 33.24679023565614 >50 0.029491378358134677 0.8106900269200529 >100 0.012734907480619041 1.042687310688619 >500 6.702582884533995E-4 0.20267243166095253 >1k 0.0016756457211334985 1.1483400793230438 >5k 3.3512914422669974E-4 0.9523822853574171 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6950 0.9342038231115304 No Hit CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1959 0.26332450208280406 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG 1871 0.2514957342506005 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC 1601 0.21520292385633963 No Hit GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC 1577 0.21197689626573862 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1372 0.18442124392935538 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 912 0.12258904844283681 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.040190927066237066 0.0 2 0.0 0.0 0.0 0.22756936295364333 0.0 3 0.0 0.0 0.0 0.32851714297619866 0.0 4 0.0 0.0 0.0 0.5658990065179199 0.0 5 0.0 0.0 0.0 1.4552072789935868 0.0 6 0.0 0.0 0.0 1.9204273411215016 0.0 7 0.0 0.0 0.0 2.4028528837326215 0.0 8 0.0 0.0 0.0 3.061500183480319 0.0 9 0.0 0.0 0.0 3.460586680000914 0.0 10 0.0 0.0 0.0 4.617655242496461 0.0 11 0.0 0.0 0.0 5.131668971932216 0.0 12 0.0 0.0 0.0 5.963446419042166 0.0 13 0.0 0.0 0.0 6.164266636557076 0.0 14 0.0 0.0 0.0 6.29532400742524 0.0 15 0.0 0.0 0.0 6.45931374328079 0.0 16 0.0 0.0 0.0 6.671021803913978 0.0 17 0.0 0.0 0.0 6.898725584683897 0.0 18 0.0 0.0 0.0 7.153850599973923 0.0 19 0.0 0.0 0.0 7.3234858841130235 0.0 20 0.0 0.0 0.0 7.499842059065877 0.0 21 0.0 0.0 0.0 7.7050980645178635 0.0 22 0.0 0.0 0.0 7.927021879187955 0.0 23 0.0 0.0 0.0 8.1545912421416 0.0 24 0.0 0.0 0.0 8.34533012343588 0.0 25 0.0 0.0 0.0 8.52504674379561 0.0 26 0.0 0.0 0.0 8.698445726790412 0.0 27 0.0 0.0 0.0 8.897249677061197 0.0 28 0.0 0.0 0.0 9.0722616738513 0.0 29 0.0 0.0 0.0 9.27765209711956 0.0 30 0.0 0.0 0.0 9.524712043433086 0.0 31 1.3441781627504036E-4 0.0 0.0 9.722978322438768 0.0 32 1.3441781627504036E-4 0.0 0.0 9.945036554925135 0.0 33 1.3441781627504036E-4 0.0 0.0 10.166960369595227 0.0 34 1.3441781627504036E-4 0.0 0.0 10.395470657262797 0.0 35 1.3441781627504036E-4 0.0 0.0 10.62384652711409 0.0 36 1.3441781627504036E-4 0.0 0.0 10.847383355579481 0.0 37 1.3441781627504036E-4 0.0 0.0 11.095384226606932 0.0 38 1.3441781627504036E-4 0.0 0.0 11.377527222968242 0.0 39 1.3441781627504036E-4 0.0 0.0 11.818417660350374 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTAGCG 35 1.2111559E-7 45.000004 1 GTCAACG 20 7.0316816E-4 45.0 1 GTATACG 20 7.0316816E-4 45.0 1 CGTTTTA 335 0.0 42.313435 1 CGTTTTT 2715 0.0 41.85083 1 TACGGGT 60 3.6379788E-12 41.250004 4 CGAATAT 110 0.0 40.90909 14 GCGATAT 55 6.002665E-11 40.90909 9 CGTTATT 560 0.0 40.178574 1 ACACGAC 125 0.0 39.6 26 TACGGCT 410 0.0 39.512196 7 TAGCACG 40 3.4560253E-7 39.375 1 CGGTCTA 40 3.4560253E-7 39.375 31 CGGTTTT 615 0.0 38.414635 1 TACGAAT 120 0.0 37.500004 12 TAGTACG 30 1.13953465E-4 37.500004 1 ACGGCTG 515 0.0 37.13592 8 GACACGA 135 0.0 36.666664 25 ACGACCA 135 0.0 36.666664 28 CTATCTC 175 0.0 36.0 5 >>END_MODULE