##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936294.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	743949
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79761247074732	31.0	31.0	34.0	30.0	34.0
2	31.905341629600954	33.0	31.0	34.0	30.0	34.0
3	31.551920897803477	33.0	31.0	34.0	30.0	34.0
4	35.454204522084176	37.0	35.0	37.0	33.0	37.0
5	31.075774011390564	37.0	35.0	37.0	0.0	37.0
6	33.2958415160179	37.0	35.0	37.0	17.0	37.0
7	29.48421867628023	35.0	32.0	37.0	0.0	37.0
8	32.47085754534249	35.0	33.0	37.0	17.0	37.0
9	36.35683763268719	39.0	35.0	39.0	32.0	39.0
10	36.805490698959204	38.0	35.0	39.0	33.0	39.0
11	36.91570793159209	39.0	37.0	39.0	33.0	39.0
12	36.8957845228638	39.0	37.0	39.0	33.0	39.0
13	36.71165899813025	39.0	35.0	39.0	33.0	39.0
14	37.893093478181974	40.0	37.0	41.0	33.0	41.0
15	37.98341418564982	40.0	37.0	41.0	33.0	41.0
16	38.05573500334028	40.0	37.0	41.0	33.0	41.0
17	37.99607634394293	40.0	37.0	41.0	33.0	41.0
18	37.83436767842957	40.0	37.0	41.0	33.0	41.0
19	37.69863660008952	39.0	36.0	41.0	33.0	41.0
20	37.575858022525736	39.0	35.0	41.0	33.0	41.0
21	37.489783573874014	39.0	35.0	41.0	33.0	41.0
22	37.4829820323705	39.0	35.0	41.0	33.0	41.0
23	37.44779413642602	39.0	35.0	41.0	33.0	41.0
24	37.357036571055275	39.0	35.0	41.0	33.0	41.0
25	37.21421764126304	39.0	35.0	41.0	32.0	41.0
26	37.046513941143814	39.0	35.0	41.0	32.0	41.0
27	36.99928624139558	39.0	35.0	41.0	32.0	41.0
28	36.87576298912963	39.0	35.0	40.0	31.0	41.0
29	36.80087747950464	39.0	35.0	40.0	31.0	41.0
30	36.65280415727422	39.0	35.0	40.0	31.0	41.0
31	36.456941268823535	39.0	35.0	40.0	30.0	41.0
32	36.264713038124924	38.0	35.0	40.0	30.0	41.0
33	35.993088235887136	38.0	35.0	40.0	29.0	41.0
34	35.69714052979438	38.0	35.0	40.0	27.0	41.0
35	35.38714481772272	38.0	34.0	40.0	25.0	41.0
36	35.194009266764255	38.0	34.0	40.0	25.0	41.0
37	35.075069662033286	38.0	34.0	40.0	24.0	41.0
38	34.80868849880839	38.0	34.0	40.0	23.0	41.0
39	34.69196409968963	38.0	34.0	40.0	23.0	41.0
40	34.39910397083671	38.0	33.0	40.0	21.0	41.0
41	34.254801068352805	38.0	33.0	40.0	21.0	41.0
42	34.122816214552344	38.0	33.0	40.0	20.0	41.0
43	33.97583302081191	38.0	33.0	40.0	18.0	41.0
44	33.771993779143465	37.0	33.0	40.0	18.0	41.0
45	33.62658192967529	37.0	33.0	40.0	18.0	41.0
46	33.42955229457933	37.0	32.0	40.0	17.0	41.0
47	33.219478754592046	37.0	32.0	40.0	15.0	41.0
48	33.075339841844	36.0	32.0	40.0	15.0	41.0
49	32.925707272944784	36.0	31.0	40.0	15.0	41.0
50	32.81289174392331	36.0	31.0	40.0	15.0	41.0
51	30.885142664349303	35.0	27.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	48.0
10	48.0
11	48.0
12	23.0
13	31.0
14	46.0
15	56.0
16	124.0
17	278.0
18	482.0
19	898.0
20	1409.0
21	2147.0
22	3335.0
23	5321.0
24	7686.0
25	11166.0
26	14267.0
27	16258.0
28	17273.0
29	17994.0
30	20240.0
31	23722.0
32	29211.0
33	39612.0
34	54070.0
35	61606.0
36	71914.0
37	106886.0
38	125475.0
39	112252.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44934666220399	19.112331624882888	23.405233423258853	16.033088289654263
2	34.94634712863382	24.732743776791153	24.02785674824484	16.293052346330192
3	31.37553783928737	24.54980112884082	28.352749986894267	15.721911044977546
4	26.551820084441275	24.24413501463138	31.084254431419357	18.11979046950799
5	22.26456383434886	37.14837979485153	24.007962911436135	16.579093459363477
6	24.584346507623504	33.81696863629093	27.852446874718563	13.746237981367
7	65.6432094135485	18.997942063232827	11.342444172920455	4.016404350298206
8	78.96428384203756	5.677539723825155	11.77325327408196	3.584923160055326
9	73.22881003939786	6.4441245300417105	13.27013007612081	7.056935354439618
10	41.12849133475547	24.703037439394368	20.262410460932134	13.906060764918024
11	30.305437603921774	24.65034565541455	26.91945281195351	18.124763928710166
12	27.788732829804193	22.20716742679942	30.91488798291281	19.089211760483582
13	24.70142442559907	21.767486749763762	33.84452428862731	19.68656453600986
14	20.95009200899524	24.01696890512656	33.11934016982347	21.91359891605473
15	20.311204128239975	24.00419921258043	35.83095077753986	19.853645881639736
16	25.051851672628096	24.422776292460906	30.747403383834104	19.77796865107689
17	25.348377375330838	23.54032332861527	30.954003567448847	20.15729572860505
18	24.80438847286575	23.968712909083823	31.316528417942628	19.910370200107803
19	24.64604428529375	25.800962162728897	29.49355399362053	20.05943955835682
20	25.780396236838815	26.1482977999836	29.935654191349137	18.135651771828446
21	25.647725852175352	25.817495554130726	30.446038639745467	18.08873995394846
22	24.302337929078472	22.646848103835072	31.056026690001598	21.994787277084853
23	23.193256526993114	24.737582818177053	31.590068674062334	20.4790919807675
24	23.914139275676156	23.852979169271013	30.88356863172072	21.349312923332107
25	24.03054510457034	26.02638084062214	28.561366437753126	21.381707617054392
26	23.099164055600585	24.433798553395462	29.546245777600344	22.92079161340361
27	22.726289033253625	24.52237989432071	30.476148230591075	22.27518284183459
28	21.100908798855837	25.356711279939887	30.59779635431999	22.944583566884287
29	23.070667478550277	25.283319152253714	29.020134444699842	22.625878924496167
30	23.68549457019231	24.289837072164893	30.196424754922717	21.82824360272008
31	24.258517720972808	24.99593386105768	28.77966097138379	21.965887446585718
32	26.278951917402942	24.62655370193387	28.011463151371935	21.083031229291255
33	24.469688110340897	24.89807769080945	28.316994847765102	22.31523935108455
34	23.95715297688417	24.696988637661992	29.888339120020323	21.45751926543352
35	22.434333536304234	26.1429210873326	29.557671291983723	21.86507408437944
36	23.005743673289434	27.61869429221627	28.422109580092187	20.953452454402115
37	23.892229171623324	26.713255881787596	28.164565044109207	21.229949902479873
38	23.45846287850377	27.452688289116594	27.850027354025613	21.238821478354026
39	24.969991222516597	24.67534736924171	28.267260255743338	22.087401152498355
40	24.770246347531888	24.575071678300528	29.92933655398421	20.725345420183373
41	22.209990200941192	25.23533199184353	29.58683995811541	22.96783784909987
42	22.096675981821335	25.016768622580308	30.402352849456076	22.484202546142278
43	22.522780459413212	24.30408536069005	29.54261649656092	23.63051768333582
44	22.69980872344744	24.50624975636771	29.29703514622642	23.496906373958428
45	22.752231671794707	24.18983021685626	28.433131841026736	24.624806270322296
46	23.474593016456772	25.56371471700345	27.93820544150204	23.023486825037736
47	21.562499579944326	25.236945005638827	30.836119142575637	22.364436271841214
48	21.956881452895296	25.443948442702393	29.477423855667528	23.12174624873479
49	22.659886632013755	24.520498044892864	30.011600257544536	22.808015065548847
50	21.49623159652073	24.82334138496053	30.005685873628433	23.67474114489031
51	21.573387423062602	24.341184677981957	29.581597663280686	24.503830235674755
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	251.0
1	737.5
2	1224.0
3	8174.0
4	15124.0
5	11343.5
6	7563.0
7	5969.0
8	4375.0
9	4150.0
10	3925.0
11	3842.0
12	3759.0
13	3565.5
14	3372.0
15	3261.0
16	3150.0
17	2957.0
18	2764.0
19	2724.0
20	2684.0
21	2791.0
22	2898.0
23	3082.0
24	3266.0
25	3962.0
26	5416.5
27	6175.0
28	7287.0
29	8399.0
30	10020.0
31	11641.0
32	13410.0
33	15179.0
34	17248.0
35	19317.0
36	20917.5
37	22518.0
38	25072.0
39	27626.0
40	28882.5
41	30139.0
42	33176.0
43	36213.0
44	39987.0
45	43761.0
46	50454.0
47	57147.0
48	55446.5
49	53746.0
50	53722.5
51	53699.0
52	51162.0
53	48625.0
54	45650.5
55	42676.0
56	41361.0
57	40046.0
58	38612.0
59	37178.0
60	35773.0
61	34368.0
62	31597.5
63	28827.0
64	25266.5
65	21706.0
66	18542.0
67	15378.0
68	13374.5
69	11371.0
70	9354.5
71	7338.0
72	6137.5
73	4937.0
74	4022.0
75	2398.0
76	1689.0
77	1332.0
78	975.0
79	744.0
80	513.0
81	389.0
82	265.0
83	220.0
84	175.0
85	132.0
86	89.0
87	68.5
88	48.0
89	38.5
90	29.0
91	19.5
92	10.0
93	14.5
94	19.0
95	10.5
96	2.0
97	2.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	743949.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.88973600603602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.62094134634506	30.103408561679107
2	8.412872773317757	6.880002935066622
3	4.286142501393949	5.257777060987483
4	2.6478524288647853	4.330799471968893
5	1.8764073154401273	3.8362899884070774
6	1.3791320094964814	3.3835406271471045
7	1.0851396404954927	3.1059751400681863
8	0.8897211677442729	2.9104370934436323
9	0.7576491815327587	2.7882067516257383
>10	4.999234316376736	33.24679023565614
>50	0.029491378358134677	0.8106900269200529
>100	0.012734907480619041	1.042687310688619
>500	6.702582884533995E-4	0.20267243166095253
>1k	0.0016756457211334985	1.1483400793230438
>5k	3.3512914422669974E-4	0.9523822853574171
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6950	0.9342038231115304	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1959	0.26332450208280406	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG	1871	0.2514957342506005	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC	1601	0.21520292385633963	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC	1577	0.21197689626573862	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1372	0.18442124392935538	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	912	0.12258904844283681	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.040190927066237066	0.0
2	0.0	0.0	0.0	0.22756936295364333	0.0
3	0.0	0.0	0.0	0.32851714297619866	0.0
4	0.0	0.0	0.0	0.5658990065179199	0.0
5	0.0	0.0	0.0	1.4552072789935868	0.0
6	0.0	0.0	0.0	1.9204273411215016	0.0
7	0.0	0.0	0.0	2.4028528837326215	0.0
8	0.0	0.0	0.0	3.061500183480319	0.0
9	0.0	0.0	0.0	3.460586680000914	0.0
10	0.0	0.0	0.0	4.617655242496461	0.0
11	0.0	0.0	0.0	5.131668971932216	0.0
12	0.0	0.0	0.0	5.963446419042166	0.0
13	0.0	0.0	0.0	6.164266636557076	0.0
14	0.0	0.0	0.0	6.29532400742524	0.0
15	0.0	0.0	0.0	6.45931374328079	0.0
16	0.0	0.0	0.0	6.671021803913978	0.0
17	0.0	0.0	0.0	6.898725584683897	0.0
18	0.0	0.0	0.0	7.153850599973923	0.0
19	0.0	0.0	0.0	7.3234858841130235	0.0
20	0.0	0.0	0.0	7.499842059065877	0.0
21	0.0	0.0	0.0	7.7050980645178635	0.0
22	0.0	0.0	0.0	7.927021879187955	0.0
23	0.0	0.0	0.0	8.1545912421416	0.0
24	0.0	0.0	0.0	8.34533012343588	0.0
25	0.0	0.0	0.0	8.52504674379561	0.0
26	0.0	0.0	0.0	8.698445726790412	0.0
27	0.0	0.0	0.0	8.897249677061197	0.0
28	0.0	0.0	0.0	9.0722616738513	0.0
29	0.0	0.0	0.0	9.27765209711956	0.0
30	0.0	0.0	0.0	9.524712043433086	0.0
31	1.3441781627504036E-4	0.0	0.0	9.722978322438768	0.0
32	1.3441781627504036E-4	0.0	0.0	9.945036554925135	0.0
33	1.3441781627504036E-4	0.0	0.0	10.166960369595227	0.0
34	1.3441781627504036E-4	0.0	0.0	10.395470657262797	0.0
35	1.3441781627504036E-4	0.0	0.0	10.62384652711409	0.0
36	1.3441781627504036E-4	0.0	0.0	10.847383355579481	0.0
37	1.3441781627504036E-4	0.0	0.0	11.095384226606932	0.0
38	1.3441781627504036E-4	0.0	0.0	11.377527222968242	0.0
39	1.3441781627504036E-4	0.0	0.0	11.818417660350374	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	35	1.2111559E-7	45.000004	1
GTCAACG	20	7.0316816E-4	45.0	1
GTATACG	20	7.0316816E-4	45.0	1
CGTTTTA	335	0.0	42.313435	1
CGTTTTT	2715	0.0	41.85083	1
TACGGGT	60	3.6379788E-12	41.250004	4
CGAATAT	110	0.0	40.90909	14
GCGATAT	55	6.002665E-11	40.90909	9
CGTTATT	560	0.0	40.178574	1
ACACGAC	125	0.0	39.6	26
TACGGCT	410	0.0	39.512196	7
TAGCACG	40	3.4560253E-7	39.375	1
CGGTCTA	40	3.4560253E-7	39.375	31
CGGTTTT	615	0.0	38.414635	1
TACGAAT	120	0.0	37.500004	12
TAGTACG	30	1.13953465E-4	37.500004	1
ACGGCTG	515	0.0	37.13592	8
GACACGA	135	0.0	36.666664	25
ACGACCA	135	0.0	36.666664	28
CTATCTC	175	0.0	36.0	5
>>END_MODULE