##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936292.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	111793
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76777615772007	31.0	31.0	34.0	30.0	34.0
2	31.90820534380507	33.0	31.0	34.0	30.0	34.0
3	31.836581896898732	33.0	31.0	34.0	30.0	34.0
4	35.58805113021388	37.0	35.0	37.0	33.0	37.0
5	31.02873167371839	37.0	35.0	37.0	0.0	37.0
6	33.28233431431306	37.0	35.0	37.0	17.0	37.0
7	29.603016289034198	35.0	32.0	37.0	0.0	37.0
8	32.653618741781685	35.0	33.0	37.0	17.0	37.0
9	36.415759484046404	39.0	35.0	39.0	32.0	39.0
10	36.791856377411825	38.0	35.0	39.0	33.0	39.0
11	36.90579911085667	39.0	37.0	39.0	33.0	39.0
12	36.687547520864456	39.0	35.0	39.0	33.0	39.0
13	36.237098923904	38.0	35.0	39.0	32.0	39.0
14	37.37821688298909	39.0	36.0	41.0	32.0	41.0
15	37.594160636175786	39.0	36.0	41.0	33.0	41.0
16	37.70954353134812	39.0	36.0	41.0	33.0	41.0
17	37.702342722710725	39.0	36.0	41.0	33.0	41.0
18	37.628259372232606	39.0	36.0	41.0	33.0	41.0
19	37.52071238807439	39.0	36.0	41.0	33.0	41.0
20	37.45584249461057	39.0	35.0	41.0	33.0	41.0
21	37.34672117216641	39.0	35.0	41.0	33.0	41.0
22	37.28062579946866	39.0	35.0	41.0	32.0	41.0
23	37.27568810211731	39.0	35.0	41.0	32.0	41.0
24	37.22268836152532	39.0	35.0	41.0	32.0	41.0
25	37.100659254157236	39.0	35.0	40.0	32.0	41.0
26	36.922025529326525	39.0	35.0	40.0	32.0	41.0
27	36.87128890002057	39.0	35.0	40.0	32.0	41.0
28	36.77334895744814	39.0	35.0	40.0	31.0	41.0
29	36.77751737586432	39.0	35.0	40.0	31.0	41.0
30	36.63798270016906	38.0	35.0	40.0	31.0	41.0
31	36.50201712092886	38.0	35.0	40.0	31.0	41.0
32	36.360684479350226	38.0	35.0	40.0	30.0	41.0
33	36.21792062114802	38.0	35.0	40.0	30.0	41.0
34	36.07024590090614	38.0	35.0	40.0	30.0	41.0
35	35.97748517349029	38.0	35.0	40.0	30.0	41.0
36	35.76307997817395	38.0	35.0	40.0	29.0	41.0
37	35.6559623590028	38.0	35.0	40.0	28.0	41.0
38	35.56350576511946	38.0	34.0	40.0	28.0	41.0
39	35.60605762435931	38.0	35.0	40.0	28.0	41.0
40	35.50538942509817	38.0	35.0	40.0	27.0	41.0
41	35.443381964881524	38.0	35.0	40.0	27.0	41.0
42	35.401035843031316	38.0	34.0	40.0	27.0	41.0
43	35.19127315663771	38.0	34.0	40.0	27.0	41.0
44	35.01530507276842	38.0	34.0	40.0	26.0	41.0
45	34.87487588668343	37.0	34.0	40.0	25.0	41.0
46	34.708595350334996	37.0	34.0	40.0	24.0	41.0
47	34.54851377098745	37.0	33.0	40.0	24.0	41.0
48	34.5104165734885	37.0	33.0	40.0	23.0	41.0
49	34.327685991072784	37.0	33.0	40.0	24.0	41.0
50	34.05011941713703	36.0	33.0	40.0	23.0	41.0
51	31.940112529407028	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	7.0
11	5.0
12	9.0
13	3.0
14	3.0
15	4.0
16	13.0
17	23.0
18	55.0
19	92.0
20	159.0
21	261.0
22	403.0
23	578.0
24	864.0
25	1121.0
26	1392.0
27	1629.0
28	1810.0
29	2138.0
30	2594.0
31	3404.0
32	4530.0
33	6752.0
34	9805.0
35	11761.0
36	10812.0
37	16337.0
38	19164.0
39	16056.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.81018489529756	21.679353805694454	27.522295671464224	10.988165627543763
2	31.0574007317095	25.288703228287996	26.320073707656118	17.33382233234639
3	31.40983782526634	23.968405893034447	27.573282763679302	17.048473518019915
4	27.94003202347195	25.574052042614476	27.57864982601773	18.907266107895843
5	22.037157961589724	39.335199878346586	22.348447577218607	16.27919458284508
6	25.396044475056577	34.953887989409	25.73685293354682	13.913214601987603
7	68.469403272119	20.0379272405249	7.234800032202374	4.257869455153722
8	81.9443077831349	5.338438005957439	8.10426413102788	4.612990079879778
9	75.36071131466193	8.299267395990805	10.640201085935614	5.6998202034116625
10	43.62348268675141	25.339690320503074	16.101187015287184	14.935639977458337
11	34.337570330879394	24.39866539049851	23.94425411251152	17.31951016611058
12	32.03778411886254	23.102519835767893	27.008846707754508	17.850849337615056
13	23.7259935774154	29.622606066569464	29.18697950676697	17.464420849248164
14	18.473428568872826	34.26958754125929	29.640496274364224	17.616487615503654
15	16.764913724472912	26.247618366087323	39.260061005608584	17.727406903831188
16	19.323213439124096	24.593668655461435	37.095345862442194	18.987772042972278
17	20.18462694444196	23.439750252699184	28.566189296288673	27.80943350657018
18	22.258102027855053	24.606191800917767	32.482355782562415	20.653350388664766
19	23.69200218260535	27.686885583176046	27.58759492991511	21.03351730430349
20	26.67072178043348	26.973066292164983	27.9552387000975	18.400973227304036
21	23.2644262163105	28.274578909234034	28.814863184635893	19.646131689819576
22	21.801007218698846	25.092805452935334	28.30946481443382	24.796722513932
23	20.42077768733284	27.31387475065523	28.4847888508225	23.780558711189432
24	21.487034071900744	24.43712933725725	31.620047766854814	22.45578882398719
25	19.57099281708157	26.4041576842915	30.707647169321874	23.317202329305058
26	18.542305868882668	29.712057105543284	28.864955766461225	22.880681259112826
27	20.014669970391708	29.9374737237573	29.985777284803163	20.06207902104783
28	17.437585537556018	27.521401161074486	33.71499109962162	21.326022201747875
29	18.49668583900602	25.77800041147478	32.70598337999696	23.019330369522244
30	21.152487186138664	27.89709552476452	30.569892569302194	20.38052471979462
31	25.06507563085345	27.286144928573346	26.930129793457553	20.71864964711565
32	23.623124882595512	28.453480987181667	26.990956499959744	20.932437630263077
33	23.990768652777902	30.248763339386187	25.11516821267879	20.645299795157122
34	19.762418040485542	27.982968522179384	28.53040888069915	23.724204556635925
35	20.654244899054504	26.672510801212958	29.371248647053033	23.301995652679505
36	23.738516722871736	29.182506954818283	27.285250418183608	19.793725904126376
37	21.412789709552477	28.552771640442604	29.313105471720053	20.721333178284866
38	19.764207061265015	29.129730841823726	26.982905906452103	24.123156190459152
39	19.93863658726396	29.21202579767964	28.173499235193617	22.675838379862782
40	21.623894161530686	27.425688549372502	28.107305466352994	22.843111822743822
41	19.729321156065225	25.843299669925667	29.182506954818283	25.244872219190828
42	22.31892873435725	25.67870975821384	27.54734196237689	24.455019545052014
43	23.419176513735206	25.254711833477945	27.094719705169375	24.23139194761747
44	22.025529326523127	26.93996940774467	28.1520309858399	22.8824702798923
45	21.215997423810077	29.178928913259327	26.106285724508687	23.498787938421906
46	23.18123675006485	28.48568336121224	27.090247153220687	21.242832735502223
47	19.870653797643858	27.055361248020898	29.866807402967986	23.20717755136726
48	21.700822055048167	25.272602041272705	29.73978692762517	23.286788976053955
49	21.65609653556126	23.337776068269033	30.87760414337212	24.12852325279758
50	20.062973531437567	25.761899224459494	29.945524317264944	24.229602926837995
51	18.994033615700445	27.250364512983815	27.492776828602867	26.262825042712873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	115.0
1	202.5
2	290.0
3	391.5
4	493.0
5	428.0
6	363.0
7	357.5
8	352.0
9	367.0
10	382.0
11	362.0
12	342.0
13	372.5
14	403.0
15	384.0
16	365.0
17	379.5
18	394.0
19	401.0
20	408.0
21	451.0
22	494.0
23	574.5
24	655.0
25	712.5
26	938.5
27	1107.0
28	1300.5
29	1494.0
30	1745.5
31	1997.0
32	2241.5
33	2486.0
34	2888.0
35	3290.0
36	3371.0
37	3452.0
38	3953.0
39	4454.0
40	5247.5
41	6041.0
42	7085.5
43	8130.0
44	9034.5
45	9939.0
46	10451.0
47	10963.0
48	11359.5
49	11756.0
50	10888.0
51	10020.0
52	8647.0
53	7274.0
54	6386.0
55	5498.0
56	4973.5
57	4449.0
58	4026.5
59	3604.0
60	3164.5
61	2725.0
62	2431.0
63	2137.0
64	1888.0
65	1639.0
66	1502.0
67	1365.0
68	1080.5
69	796.0
70	634.0
71	472.0
72	408.5
73	345.0
74	279.5
75	174.5
76	135.0
77	105.5
78	76.0
79	61.0
80	46.0
81	32.0
82	18.0
83	16.0
84	14.0
85	14.0
86	14.0
87	13.0
88	12.0
89	8.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	111793.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.08869070514254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.47315795738602	34.02985875680946
2	9.45919136610721	8.530051076543256
3	4.043169463952704	5.469036522859213
4	2.277506646034202	4.107591709677708
5	1.5275959211205015	3.443865000491981
6	1.095107725270801	2.9626184108128415
7	0.7598301789469508	2.398182354888052
8	0.6725389834543506	2.4259121769699354
9	0.5039082648891006	2.0448507509414724
>10	4.160219021545054	33.40012344243378
>50	0.017855017259850017	0.561752524755575
>100	0.00991945403325001	0.6261572728167237
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGC	186	0.16637893249130087	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	153	0.13686008962994106	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	125	0.1118137987172721	No Hit
GATTCTGGGGATTGGAAAGTTTTATTCTTTTATTGAACACTTCGGCCATAT	120	0.10734124676858121	No Hit
CTGTCTCTTATACACATCTGACGCTGGCATCATCGTATGCCGTCTTCTGCT	116	0.1037632052096285	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16548442210156272	0.0
2	0.0	0.0	0.0	0.6986126143855161	0.0
3	0.0	0.0	0.0	0.9669657313069692	0.0
4	0.0	0.0	0.0	1.3274534183714544	0.0
5	0.0	0.0	0.0	2.236275974345442	0.0
6	0.0	0.0	0.0	2.8821124757364056	0.0
7	0.0	0.0	0.0	3.5521007576503	0.0
8	0.0	0.0	0.0	4.960060111098191	0.0
9	0.0	0.0	0.0	5.501238896889787	0.0
10	0.0	0.0	0.0	6.664102403549418	0.0
11	0.0	0.0	0.0	8.094424516740762	0.0
12	0.0	0.0	0.0	9.22419113898008	0.0
13	0.0	0.0	0.0	9.743007165028223	0.0
14	0.0	0.0	0.0	9.909386097519523	0.0
15	0.0	0.0	0.0	10.2931310547172	0.0
16	0.0	0.0	0.0	11.006950345728265	0.0
17	0.0	0.0	0.0	11.846895601692413	0.0
18	0.0	0.0	0.0	12.926569642106394	0.0
19	0.0	0.0	0.0	13.440913116205845	0.0
20	0.0	0.0	0.0	13.966885225371893	0.0
21	0.0	0.0	0.0	14.634189976116573	0.0
22	0.0	0.0	0.0	15.26839784244094	0.0
23	0.0	0.0	0.0	15.960748884098289	0.0
24	0.0	0.0	0.0	16.440206452997952	0.0
25	0.0	0.0	0.0	16.89193419981573	0.0
26	0.0	0.0	0.0	17.32845527000796	0.0
27	0.0	0.0	0.0	17.713989247985115	0.0
28	0.0	0.0	0.0	18.095050674013578	0.0
29	0.0	0.0	0.0	18.52620468186738	0.0
30	0.0	0.0	0.0	19.035181093628402	0.0
31	0.0	0.0	0.0	19.5271618079844	0.0
32	0.0	0.0	0.0	19.960999347007416	0.0
33	0.0	0.0	0.0	20.400203948368862	0.0
34	0.0	0.0	0.0	20.82599089388423	0.0
35	0.0	0.0	0.0	21.385059887470593	0.0
36	0.0	0.0	0.0	21.79385113558094	0.0
37	0.0	0.0	0.0	22.282253808377984	0.0
38	0.0	0.0	0.0	22.74561019026236	0.0
39	0.0	0.0	0.0	23.21254461370569	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTAGC	20	7.0059526E-4	45.0	25
ACTATCA	20	7.0059526E-4	45.0	11
ATCTCAT	20	7.0059526E-4	45.0	15
GGAGCCT	20	7.0059526E-4	45.0	8
CATATCA	20	7.0059526E-4	45.0	29
GTATTGG	20	7.0059526E-4	45.0	2
CTATCTC	45	3.783498E-10	45.0	5
CATGCAT	20	7.0059526E-4	45.0	25
TCTTCTA	20	7.0059526E-4	45.0	23
CAGTTTA	20	7.0059526E-4	45.0	26
TATGGGC	45	3.783498E-10	45.0	4
CGCATGG	20	7.0059526E-4	45.0	2
GCTATGG	40	6.721166E-9	45.0	2
GCTATCT	20	7.0059526E-4	45.0	4
CGCGCAT	20	7.0059526E-4	45.0	43
CAATTTT	25	3.868166E-5	45.0	41
CGACAAT	20	7.0059526E-4	45.0	20
AGGCGAT	45	3.783498E-10	45.0	7
GCGCATT	20	7.0059526E-4	45.0	44
GCGCATG	20	7.0059526E-4	45.0	1
>>END_MODULE