##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936291.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	264820
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.816078846008608	31.0	31.0	34.0	30.0	34.0
2	31.969473604712636	33.0	31.0	34.0	30.0	34.0
3	31.906781965108376	33.0	31.0	34.0	30.0	34.0
4	35.64700173702892	37.0	35.0	37.0	33.0	37.0
5	31.273329053696852	37.0	35.0	37.0	0.0	37.0
6	33.41773657578733	37.0	35.0	37.0	17.0	37.0
7	29.364190771089795	35.0	32.0	37.0	0.0	37.0
8	32.45296805377237	35.0	33.0	37.0	17.0	37.0
9	36.30995015482215	39.0	35.0	39.0	32.0	39.0
10	36.72588173098708	38.0	35.0	39.0	33.0	39.0
11	36.87838909447927	39.0	37.0	39.0	33.0	39.0
12	36.82838154217959	39.0	35.0	39.0	33.0	39.0
13	36.535627973718	39.0	35.0	39.0	32.0	39.0
14	37.7490068725927	40.0	36.0	41.0	33.0	41.0
15	37.878381542179596	40.0	36.0	41.0	33.0	41.0
16	37.95141983233895	40.0	36.0	41.0	33.0	41.0
17	37.90776376406616	40.0	36.0	41.0	33.0	41.0
18	37.81642625179367	40.0	36.0	41.0	33.0	41.0
19	37.742466581073934	40.0	36.0	41.0	33.0	41.0
20	37.66757042519447	39.0	36.0	41.0	33.0	41.0
21	37.57501699267427	39.0	36.0	41.0	33.0	41.0
22	37.56464390907031	39.0	36.0	41.0	33.0	41.0
23	37.54747753190846	39.0	36.0	41.0	33.0	41.0
24	37.47293633411374	39.0	35.0	41.0	33.0	41.0
25	37.330617022883466	39.0	35.0	41.0	32.0	41.0
26	37.18939279510611	39.0	35.0	41.0	32.0	41.0
27	37.111234045766935	39.0	35.0	41.0	32.0	41.0
28	37.01099237217733	39.0	35.0	41.0	31.0	41.0
29	36.94896155879465	39.0	35.0	41.0	31.0	41.0
30	36.823223321501395	39.0	35.0	40.0	31.0	41.0
31	36.713635677063664	39.0	35.0	40.0	31.0	41.0
32	36.54498904916547	39.0	35.0	40.0	30.0	41.0
33	36.369333887168644	39.0	35.0	40.0	30.0	41.0
34	36.26460614757193	39.0	35.0	40.0	30.0	41.0
35	36.05847368023563	39.0	35.0	40.0	29.0	41.0
36	35.886303904538934	38.0	35.0	40.0	29.0	41.0
37	35.77378219167736	38.0	35.0	40.0	28.0	41.0
38	35.643995921758176	38.0	35.0	40.0	27.0	41.0
39	35.60928932860056	38.0	35.0	40.0	27.0	41.0
40	35.44764368250132	38.0	34.0	40.0	26.0	41.0
41	35.31282380484858	38.0	34.0	40.0	26.0	41.0
42	35.247677667849864	38.0	34.0	40.0	26.0	41.0
43	35.02219620874556	38.0	34.0	40.0	24.0	41.0
44	34.87124084283664	38.0	34.0	40.0	24.0	41.0
45	34.722211313344914	38.0	34.0	40.0	24.0	41.0
46	34.54392039876142	38.0	33.0	40.0	23.0	41.0
47	34.38090023412129	37.0	33.0	40.0	23.0	41.0
48	34.29984517785666	37.0	33.0	40.0	23.0	41.0
49	34.120017370289254	37.0	33.0	40.0	22.0	41.0
50	33.952182614606144	37.0	33.0	40.0	22.0	41.0
51	31.79782116154369	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	14.0
10	16.0
11	6.0
12	9.0
13	11.0
14	8.0
15	24.0
16	45.0
17	85.0
18	147.0
19	244.0
20	384.0
21	645.0
22	963.0
23	1410.0
24	2046.0
25	2589.0
26	3249.0
27	3820.0
28	4507.0
29	5470.0
30	6557.0
31	8140.0
32	10393.0
33	14602.0
34	20836.0
35	24497.0
36	26276.0
37	39435.0
38	46488.0
39	41893.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.87848349822521	22.660297560607205	26.316365833396272	12.144853107771317
2	31.730609470583794	26.83520882108602	23.534853863001285	17.8993278453289
3	32.13767842307983	24.51098859602749	26.426251793671174	16.925081187221508
4	30.152934068423836	23.900007552299677	26.821614681670567	19.12544369760592
5	22.83664375802432	38.29997734310097	21.1819348991768	17.68144399969791
6	25.813005060040783	34.585756362812475	24.65146136998716	14.949777207159581
7	67.6735140850389	20.024167358960803	7.4979231175893055	4.804395438410996
8	81.01389623140246	6.272940110263575	8.457065176346196	4.256098481987766
9	74.36334113737632	8.182916698134582	10.135186164186996	7.318556000302092
10	41.569367872517184	28.16025979910883	16.968506910354204	13.301865418019787
11	33.344535911184956	24.658636054678652	25.184276112076127	16.81255192206027
12	32.15882486217053	21.59013669662412	27.42013443093422	18.830904010271126
13	25.163884902952947	25.36553130428215	29.41696246507061	20.05362132769428
14	20.034740578506156	29.410165395362885	28.613397779623895	21.94169624650706
15	19.389018956272185	26.534627294011027	35.029453968733485	19.04689978098331
16	22.469601993807114	24.80061928857337	32.44694509478136	20.282833622838154
17	21.791405482969566	24.860282456007855	28.50577750925157	24.842534551771013
18	22.358960803564685	27.38917000226569	28.53447624801752	21.717392946152103
19	25.13443093421947	27.60441054301035	26.636583339626917	20.624575183143268
20	26.618457820406316	26.340533192357075	28.613397779623895	18.427611207612717
21	24.50607960123858	27.004380333811646	27.537572690884378	20.951967374065404
22	22.4495883996677	25.24242881957556	27.76602975606072	24.54195302469602
23	23.25843969488709	26.710218261460618	27.8660977267578	22.165244316894494
24	22.83702137300808	24.684313873574503	29.47813609244015	23.000528660977267
25	21.916773657578734	27.098784079752285	27.76112076127181	23.223321501397177
26	20.404048032625933	28.6526697379352	27.684842534551773	23.25843969488709
27	21.850691035420287	28.128540140472776	27.651234800996903	22.369534023110038
28	20.143116078846006	28.116456460992374	29.29461521033155	22.445812249830073
29	23.012234725473906	25.657050071746845	28.53107771316366	22.799637489615588
30	21.857488105128013	25.917982025526776	28.01601087531153	24.208518994033685
31	23.989879918435165	26.092817763008835	25.837172419001586	24.080129899554414
32	25.124612944641644	26.149837625556984	26.85975379503059	21.865795634770787
33	22.8105883241447	26.491956800845855	27.761498376255574	22.93595649875387
34	22.46129446416434	25.40215995770712	28.759157163356242	23.377388414772298
35	22.184125066082622	24.768899629937316	27.6391511215165	25.40782418246356
36	22.397099916924702	27.62442413714976	26.79858016766105	23.17989577826448
37	21.774790423684014	27.826070538478966	28.387206404350124	22.011932633486897
38	20.640435012461296	27.31817838531833	26.895627218488027	25.145759383732347
39	22.78226720036251	24.919945623442338	29.180953100219014	23.116834075976133
40	22.4616720791481	24.189260629861792	30.35420285476928	22.99486443622083
41	20.29114115248093	26.025979910882864	29.42791329959973	24.254965637036477
42	21.35979155652896	26.060720489389016	28.113057926138506	24.46643002794351
43	22.47224529869345	25.839060493920396	27.257004757948796	24.431689449437354
44	21.667170153311684	25.690280190317953	27.984291216675476	24.658258439694887
45	22.027414847821163	25.191073181783853	27.80983309417718	24.97167887621781
46	21.96661883543539	26.95000377614984	27.32459784004229	23.75877954837248
47	20.765803187070464	25.884374291971906	29.739445661203835	23.610376859753796
48	21.42436371875236	24.54346348463107	29.390529416207233	24.641643380409334
49	21.247262291367722	24.065402915187676	30.22128238048486	24.466052412959748
50	19.473604712634998	24.66732119930519	29.78249376935277	26.076580318707048
51	19.135261687183746	25.206177781134354	28.099463786723057	27.55909674495884
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	91.0
1	311.0
2	531.0
3	836.5
4	1142.0
5	879.5
6	617.0
7	569.5
8	522.0
9	532.0
10	542.0
11	540.5
12	539.0
13	569.0
14	599.0
15	624.0
16	649.0
17	672.0
18	695.0
19	736.5
20	778.0
21	952.0
22	1126.0
23	1321.5
24	1517.0
25	1613.0
26	2157.5
27	2606.0
28	3232.5
29	3859.0
30	4370.5
31	4882.0
32	5547.0
33	6212.0
34	6958.5
35	7705.0
36	8178.0
37	8651.0
38	9567.5
39	10484.0
40	11528.0
41	12572.0
42	14315.0
43	16058.0
44	18099.0
45	20140.0
46	21836.0
47	23532.0
48	22646.5
49	21761.0
50	21539.5
51	21318.0
52	18901.5
53	16485.0
54	15365.5
55	14246.0
56	13320.0
57	12394.0
58	12284.0
59	12174.0
60	11743.5
61	11313.0
62	10336.5
63	9360.0
64	7950.5
65	6541.0
66	5312.5
67	4084.0
68	3314.0
69	2544.0
70	2203.0
71	1862.0
72	1498.5
73	1135.0
74	930.5
75	588.5
76	451.0
77	380.0
78	309.0
79	212.5
80	116.0
81	126.5
82	137.0
83	92.5
84	48.0
85	35.5
86	23.0
87	22.0
88	21.0
89	13.0
90	5.0
91	4.0
92	3.0
93	4.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	264820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.68609082718973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.24197187866831	27.947594856334483
2	9.551337971969716	7.390078566096133
3	4.301554601631754	4.992309960505258
4	2.6912030233809636	4.164484983876942
5	1.8256933001951798	3.5314468416971243
6	1.389999460891481	3.2264187236275554
7	1.006275320650114	2.725020091628085
8	0.8453911580112133	2.6163903298659923
9	0.6175552415338986	2.1501718348308745
>10	5.463065541559748	38.70734542539468
>50	0.05196257694540006	1.2826807167028775
>100	0.012990644236350015	1.066581102285485
>500	9.99280325873078E-4	0.19947656715451623
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG	516	0.19484933162147874	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC	414	0.15633260327769807	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC	392	0.14802507363492184	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	326	0.12310248470659316	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	314	0.1185711049014425	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034740578506155126	0.0
2	0.0	0.0	0.0	0.32852503587342347	0.0
3	0.0	0.0	0.0	0.5139339929008383	0.0
4	0.0	0.0	0.0	0.8228230496186089	0.0
5	0.0	0.0	0.0	1.7823427233592628	0.0
6	0.0	0.0	0.0	2.343100974246658	0.0
7	0.0	0.0	0.0	2.9548372479419984	0.0
8	0.0	0.0	0.0	3.920021146439091	0.0
9	0.0	0.0	0.0	4.349369382977117	0.0
10	0.0	0.0	0.0	5.507892153160637	0.0
11	0.0	0.0	0.0	6.638471414545729	0.0
12	0.0	0.0	0.0	7.82644815346273	0.0
13	0.0	0.0	0.0	8.211237821916773	0.0
14	0.0	0.0	0.0	8.369080885129522	0.0
15	0.0	0.0	0.0	8.670040027188278	0.0
16	0.0	0.0	0.0	9.227399743221811	0.0
17	0.0	0.0	0.0	9.876897515293408	0.0
18	0.0	0.0	0.0	10.62948417793218	0.0
19	0.0	0.0	0.0	11.033154595574352	0.0
20	0.0	0.0	0.0	11.4421116229892	0.0
21	0.0	0.0	0.0	11.922060267351409	0.0
22	0.0	0.0	0.0	12.478664753417416	0.0
23	0.0	0.0	0.0	13.00241673589608	0.0
24	0.0	0.0	0.0	13.409485688392115	0.0
25	0.0	0.0	0.0	13.778793142511894	0.0
26	0.0	0.0	0.0	14.139415452005135	0.0
27	0.0	0.0	0.0	14.488709311985499	0.0
28	0.0	0.0	0.0	14.849709236462504	0.0
29	0.0	0.0	0.0	15.263952873650027	0.0
30	0.0	0.0	0.0	15.658182916698134	0.0
31	0.0	0.0	0.0	16.072048938901897	0.0
32	0.0	0.0	0.0	16.474208896609017	0.0
33	0.0	0.0	0.0	16.83369836115097	0.0
34	0.0	0.0	0.0	17.210180499962238	0.0
35	0.0	0.0	0.0	17.60214485310777	0.0
36	0.0	0.0	0.0	17.977116531983988	0.0
37	0.0	0.0	0.0	18.33811645646099	0.0
38	0.0	0.0	0.0	18.736500264330488	0.0
39	0.0	0.0	0.0	19.14168114190771	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTCAC	20	7.0234435E-4	45.000004	10
ATAATCG	20	7.0234435E-4	45.000004	22
TACGAGC	20	7.0234435E-4	45.000004	28
CGCACTG	20	7.0234435E-4	45.000004	3
ACGAGCC	20	7.0234435E-4	45.000004	29
GTAACGG	20	7.0234435E-4	45.000004	2
ATCGCAC	20	7.0234435E-4	45.000004	25
AGTCGTT	20	7.0234435E-4	45.000004	37
TAATCGC	20	7.0234435E-4	45.000004	23
TCAGACG	25	3.8826496E-5	45.0	22
TTTGACG	25	3.8826496E-5	45.0	1
ATCATCG	25	3.8826496E-5	45.0	42
TTCGAAG	25	3.8826496E-5	45.0	1
TAGTATG	25	3.8826496E-5	45.0	32
TACGGGC	30	2.1591532E-6	44.999996	4
AGGCGAT	60	0.0	44.999996	7
CGAATAT	30	2.1591532E-6	44.999996	14
CATACGA	100	0.0	42.75	18
ATGGGCG	70	0.0	41.785713	5
AATGGGC	60	3.6379788E-12	41.249996	4
>>END_MODULE