##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936290.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	633592
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.797249649616788	31.0	31.0	34.0	30.0	34.0
2	31.90416703493731	33.0	31.0	34.0	30.0	34.0
3	31.596420093688053	33.0	31.0	34.0	30.0	34.0
4	35.46493800426773	37.0	35.0	37.0	33.0	37.0
5	31.13652161012134	37.0	35.0	37.0	0.0	37.0
6	33.356342883117215	37.0	35.0	37.0	17.0	37.0
7	29.570719011603682	35.0	32.0	37.0	0.0	37.0
8	32.61998257553757	35.0	33.0	37.0	17.0	37.0
9	36.50878641144459	39.0	35.0	39.0	32.0	39.0
10	36.85216827232667	38.0	37.0	39.0	33.0	39.0
11	36.96357908559452	39.0	37.0	39.0	33.0	39.0
12	37.03662609376381	39.0	37.0	39.0	33.0	39.0
13	36.91629313501433	39.0	37.0	39.0	33.0	39.0
14	38.135097981035116	40.0	37.0	41.0	33.0	41.0
15	38.21195659036099	40.0	37.0	41.0	33.0	41.0
16	38.26018636598947	40.0	37.0	41.0	33.0	41.0
17	38.21504532885516	40.0	37.0	41.0	33.0	41.0
18	38.06704630108966	40.0	37.0	41.0	33.0	41.0
19	37.94414228715009	40.0	37.0	41.0	33.0	41.0
20	37.82549653404715	40.0	36.0	41.0	33.0	41.0
21	37.75414777964368	39.0	36.0	41.0	33.0	41.0
22	37.762020353792344	39.0	36.0	41.0	33.0	41.0
23	37.73456735564843	39.0	36.0	41.0	33.0	41.0
24	37.66577860831576	39.0	36.0	41.0	33.0	41.0
25	37.54768368287478	39.0	35.0	41.0	33.0	41.0
26	37.39994823166959	39.0	35.0	41.0	33.0	41.0
27	37.38807308173083	39.0	35.0	41.0	33.0	41.0
28	37.277142072500915	39.0	35.0	41.0	32.0	41.0
29	37.26836986578113	39.0	35.0	41.0	32.0	41.0
30	37.122796689352136	39.0	35.0	41.0	32.0	41.0
31	36.953119673228194	39.0	35.0	41.0	31.0	41.0
32	36.82354733014306	39.0	35.0	41.0	31.0	41.0
33	36.59893275167616	39.0	35.0	41.0	30.0	41.0
34	36.33973913812043	39.0	35.0	40.0	30.0	41.0
35	36.1733591964545	39.0	35.0	41.0	29.0	41.0
36	35.97419001502544	39.0	35.0	40.0	28.0	41.0
37	35.858964443995504	39.0	35.0	40.0	27.0	41.0
38	35.68761284864708	39.0	35.0	40.0	26.0	41.0
39	35.63219863887171	39.0	35.0	40.0	26.0	41.0
40	35.53161182590689	39.0	35.0	40.0	25.0	41.0
41	35.412446811197114	39.0	35.0	40.0	25.0	41.0
42	35.38829246581396	39.0	35.0	40.0	25.0	41.0
43	35.24371519842422	38.0	34.0	40.0	24.0	41.0
44	35.104875061553805	38.0	34.0	40.0	24.0	41.0
45	34.9841996111062	38.0	34.0	40.0	23.0	41.0
46	34.88825458654781	38.0	34.0	40.0	23.0	41.0
47	34.694301695728484	38.0	34.0	40.0	23.0	41.0
48	34.58184762433869	38.0	34.0	40.0	22.0	41.0
49	34.40044855364335	38.0	33.0	40.0	22.0	41.0
50	34.233773469361985	38.0	33.0	40.0	20.0	41.0
51	32.21178771196606	36.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	10.0
9	28.0
10	22.0
11	19.0
12	13.0
13	7.0
14	13.0
15	31.0
16	61.0
17	133.0
18	254.0
19	478.0
20	851.0
21	1401.0
22	2224.0
23	3446.0
24	5166.0
25	7753.0
26	9760.0
27	10615.0
28	10982.0
29	11759.0
30	13700.0
31	17181.0
32	21737.0
33	29580.0
34	41303.0
35	50228.0
36	61836.0
37	96566.0
38	120240.0
39	116176.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.499854796146415	20.508308185709414	26.137798457051225	14.854038561092944
2	31.954633265571537	25.963553832750414	25.571503428073587	16.510309473604465
3	30.799946969027385	24.789138751751917	29.749586484677838	14.661327794542862
4	26.137324966224323	24.39172211770351	31.909967297566887	17.560985618505285
5	21.72549527140494	37.38999229788255	25.051610500132576	15.832901930579931
6	24.156870667558934	33.644837687344534	28.38388110960997	13.81441053548656
7	67.44908395308022	18.54079596964608	10.48056162325282	3.529558454020884
8	81.72830465031124	4.511894089571838	10.473617091124888	3.2861841689920324
9	76.22144850313767	6.517443402063157	11.698380030050885	5.562728064748292
10	36.08252629452392	33.56608037980278	18.395434285786436	11.955959039886867
11	24.803501306834683	25.89473983257364	32.03244359145948	17.269315269132186
12	23.24839960100506	23.472045101579567	34.52363666207906	18.755918635336304
13	22.08203386406394	23.991622368969306	36.55096655260799	17.375377214358767
14	18.824101314410534	27.33446760691423	34.21949772093082	19.621933357744417
15	18.433944873041327	26.937208803141456	36.945857902246246	17.68298842157098
16	22.484185406381393	27.931223879089384	31.543958888369804	18.04063182615942
17	22.814208512733746	26.154844126819782	31.37366002095986	19.657287339486608
18	23.100354802459627	26.271165039962625	32.43775173928964	18.19072841828811
19	22.31120342428566	27.782389929165774	30.576617129004152	19.329789517544413
20	22.49034078713115	29.072968093031477	31.20525511685754	17.231436002979837
21	22.415529236480257	27.176637331279434	33.179869695324435	17.227963736915868
22	22.206719781815426	23.68480031313527	33.242686145027086	20.86579376002222
23	20.64609401633859	26.914007752623135	32.8689440523239	19.570954178714377
24	20.890573113296885	26.43830730185987	32.64766600588391	20.02345357895933
25	20.93018851248122	28.98410964784909	30.50906577103246	19.576636068637228
26	20.262250785994773	27.867460447732928	30.663897271430194	21.206391494842105
27	20.67576610815793	27.135443629338756	32.1558668670059	20.032923395497416
28	18.56352352933749	27.093776436571172	33.18034318615134	21.16235684794
29	20.607109938256794	25.794833268096816	32.99094685538959	20.607109938256794
30	21.695507519034333	25.344701321986392	33.73148650866804	19.22830465031124
31	23.183689187994798	25.510265281127285	31.085619767926364	20.220425762951553
32	22.23686536446167	26.125172035000443	32.31906337201227	19.318899228525613
33	23.090411495094635	25.56298059318931	30.59666157400977	20.749946337706284
34	20.949443806108665	25.67630273109509	32.54065707900352	20.833596383792724
35	19.563693986035176	26.443673531231454	32.29018043157111	21.702452051162265
36	22.425630374120885	27.461678809075874	30.149212742585135	19.96347807421811
37	20.08832182224523	27.827845048548593	31.874613315824696	20.20921981338148
38	21.604755110544325	27.453471634742865	29.818084824303337	21.123688430409473
39	21.83045240470208	26.390326898066895	30.184093233500427	21.595127463730606
40	22.07998207048069	25.294511294334526	31.185526332403185	21.4399803027816
41	19.694219623985152	27.095986060430054	30.101547999343424	23.108246316241367
42	20.5245647040998	26.60166163714188	31.10108713493857	21.772686523819747
43	21.20449753153449	26.52179951767068	30.737288349600373	21.53641460119446
44	21.478964380863395	25.706448313741337	30.480814151693835	22.333773153701436
45	20.866740741676033	25.1941312390308	30.56399070695337	23.375137312339803
46	22.15542494223412	25.961186378615892	29.670513516584805	22.212875162565187
47	19.466312705968512	25.888110960996986	32.58958446445031	22.055991868584197
48	20.755470397353502	26.380541420977536	30.644168486975847	22.219819694693115
49	21.471230697357292	25.134787055392117	31.7743910907966	21.619591156453996
50	19.799650248109195	25.417145418502756	31.565739466407404	23.217464866980645
51	20.383148777131023	24.83506736196164	30.08434449929923	24.6974393616081
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	275.0
1	549.5
2	824.0
3	5009.5
4	9195.0
5	7283.5
6	5372.0
7	4313.0
8	3254.0
9	3082.5
10	2911.0
11	2935.5
12	2960.0
13	2878.0
14	2796.0
15	2698.0
16	2600.0
17	2598.5
18	2597.0
19	2613.5
20	2630.0
21	3034.5
22	3439.0
23	3666.5
24	3894.0
25	4903.0
26	7001.5
27	8091.0
28	9746.5
29	11402.0
30	13057.0
31	14712.0
32	16717.0
33	18722.0
34	20919.5
35	23117.0
36	25178.0
37	27239.0
38	29413.5
39	31588.0
40	33448.0
41	35308.0
42	38319.5
43	41331.0
44	44934.0
45	48537.0
46	55067.5
47	61598.0
48	58696.0
49	55794.0
50	52529.0
51	49264.0
52	43659.5
53	38055.0
54	34336.5
55	30618.0
56	26840.0
57	23062.0
58	20939.5
59	18817.0
60	16722.0
61	14627.0
62	12661.0
63	10695.0
64	9090.0
65	7485.0
66	6256.5
67	5028.0
68	4264.0
69	3500.0
70	2904.0
71	2308.0
72	1993.0
73	1678.0
74	1390.5
75	812.0
76	521.0
77	432.5
78	344.0
79	262.0
80	180.0
81	140.5
82	101.0
83	74.5
84	48.0
85	34.5
86	21.0
87	16.0
88	11.0
89	12.0
90	13.0
91	8.5
92	4.0
93	4.5
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	633592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.6036789371671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.15380398413754	32.01828535846986
2	9.080349255349672	7.737125686245237
3	3.8952555627602607	4.97856652122157
4	2.35336722736284	4.010484071032703
5	1.619270972346457	3.4493450309061426
6	1.1760991641382021	3.0063690712328737
7	0.9331189765024155	2.782801089956082
8	0.7333579756255353	2.4994998191648903
9	0.5839212063584838	2.238947244027013
>10	4.424457240344525	32.643318586817806
>50	0.0294217846889042	0.8246897093874805
>100	0.012991437241200441	1.1974767951843868
>500	0.0026747076673059723	0.7218069752375709
>1k	0.0019105054766471233	1.8912840411163958
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4124	0.6508920567178879	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTC	2643	0.4171454185027589	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCG	2015	0.31802800540410864	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1427	0.22522380333085015	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGC	1409	0.22238285836942384	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	833	0.13147261960378287	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCT	705	0.11127034432252933	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	688	0.10858722963673784	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTT	638	0.10069571585499817	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10432581219459841	0.0
2	0.0	0.0	0.0	0.4416091112261518	0.0
3	0.0	0.0	0.0	0.6458414878975745	0.0
4	0.0	0.0	0.0	0.9845452594098411	0.0
5	0.0	0.0	0.0	2.149174863318981	0.0
6	0.0	0.0	0.0	2.8357365623303323	0.0
7	0.0	0.0	0.0	3.451905958408566	0.0
8	0.0	0.0	0.0	4.445289713253955	0.0
9	0.0	0.0	0.0	4.884531370345585	0.0
10	0.0	0.0	0.0	6.102191946868016	0.0
11	0.0	0.0	0.0	6.998510082198008	0.0
12	0.0	0.0	0.0	8.045398300483592	0.0
13	0.0	0.0	0.0	8.430188512481218	0.0
14	0.0	0.0	0.0	8.645311178171442	0.0
15	0.0	0.0	0.0	8.915200949506938	0.0
16	0.0	0.0	0.0	9.314985037689869	0.0
17	0.0	0.0	0.0	9.85002967209182	0.0
18	0.0	0.0	0.0	10.439525751587773	0.0
19	0.0	0.0	0.0	10.821475018623973	0.0
20	0.0	0.0	0.0	11.201056831525651	0.0
21	0.0	0.0	0.0	11.645980378540132	0.0
22	0.0	0.0	0.0	12.138884329347594	0.0
23	0.0	0.0	0.0	12.636207527872827	0.0
24	0.0	0.0	0.0	13.015158019671965	0.0
25	0.0	0.0	0.0	13.370276139850251	0.0
26	0.0	0.0	0.0	13.715766613214813	0.0
27	0.0	0.0	0.0	14.08508945820023	0.0
28	0.0	0.0	0.0	14.449677394916602	0.0
29	0.0	0.0	0.0	14.857195166605639	0.0
30	0.0	0.0	0.0	15.32247881917701	0.0
31	0.0	0.0	0.0	15.721158095430498	0.0
32	0.0	0.0	0.0	16.11841689920327	0.0
33	0.0	0.0	0.0	16.50383843230344	0.0
34	0.0	0.0	0.0	16.901886387454386	0.0
35	0.0	0.0	0.0	17.316190860995718	0.0
36	0.0	0.0	0.0	17.698297958307556	0.0
37	0.0	0.0	0.0	18.097766385939217	0.0
38	0.0	0.0	0.0	18.53842851551156	0.0
39	0.0	0.0	0.0	19.11640298488617	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAAT	25	3.888816E-5	45.000004	13
GGATCGA	55	1.8189894E-12	45.000004	8
TTACGCG	25	3.888816E-5	45.000004	1
TCGAATG	25	3.888816E-5	45.000004	11
TAGTACG	45	3.8380676E-10	45.000004	1
CTCCGAA	35	1.2107739E-7	45.0	20
TAACGCG	20	7.0308876E-4	45.0	1
TCTAGCG	40	6.8066583E-9	45.0	1
AGTGCGA	20	7.0308876E-4	45.0	1
ATCGAAT	20	7.0308876E-4	45.0	10
CGAATAT	65	0.0	44.999996	14
CGTTATT	475	0.0	43.578945	1
GTTACGG	80	0.0	42.1875	2
CGGTTTT	580	0.0	41.89655	1
CGTTTTT	1830	0.0	41.434425	1
TACGCGG	60	3.6379788E-12	41.249996	2
TTGCACG	45	1.925946E-8	40.000004	1
CGTTTTA	250	0.0	39.6	1
GGCGATA	205	0.0	39.512196	8
GTTTACG	40	3.454952E-7	39.375	1
>>END_MODULE