##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936289.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434082
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.860058698586904	31.0	31.0	34.0	30.0	34.0
2	32.01061780953829	33.0	31.0	34.0	30.0	34.0
3	31.97047332070899	33.0	31.0	34.0	30.0	34.0
4	35.684836044802594	37.0	35.0	37.0	33.0	37.0
5	30.949880437336724	37.0	35.0	37.0	0.0	37.0
6	33.276120640800585	37.0	35.0	37.0	17.0	37.0
7	29.771692905948644	35.0	32.0	37.0	0.0	37.0
8	32.79307135518174	35.0	35.0	37.0	17.0	37.0
9	36.6126814749287	39.0	35.0	39.0	32.0	39.0
10	36.753429075612445	38.0	35.0	39.0	33.0	39.0
11	36.938428683981364	39.0	37.0	39.0	33.0	39.0
12	36.993692435991356	39.0	37.0	39.0	33.0	39.0
13	36.807130910749585	39.0	37.0	39.0	33.0	39.0
14	38.006273008325614	40.0	37.0	41.0	33.0	41.0
15	38.13538686239006	40.0	37.0	41.0	33.0	41.0
16	38.186835666993794	40.0	37.0	41.0	33.0	41.0
17	38.17663252565183	40.0	37.0	41.0	34.0	41.0
18	38.09331877387222	40.0	37.0	41.0	33.0	41.0
19	38.047426523099325	40.0	37.0	41.0	34.0	41.0
20	37.988283319741434	40.0	37.0	41.0	33.0	41.0
21	37.90880524877788	40.0	37.0	41.0	33.0	41.0
22	37.94020254237679	40.0	37.0	41.0	33.0	41.0
23	37.86370777871462	40.0	36.0	41.0	33.0	41.0
24	37.82077349440889	40.0	36.0	41.0	33.0	41.0
25	37.67562119599523	39.0	36.0	41.0	33.0	41.0
26	37.51344907183435	39.0	36.0	41.0	33.0	41.0
27	37.45390502255334	39.0	36.0	41.0	32.0	41.0
28	37.37791016443898	39.0	36.0	41.0	32.0	41.0
29	37.33061495293516	39.0	36.0	41.0	32.0	41.0
30	37.15351707741855	39.0	36.0	41.0	31.0	41.0
31	37.05901189176239	39.0	35.0	41.0	31.0	41.0
32	36.96829861639045	39.0	35.0	41.0	31.0	41.0
33	36.73645762782147	39.0	35.0	41.0	30.0	41.0
34	36.656613266617825	39.0	35.0	41.0	30.0	41.0
35	36.51872918020098	39.0	35.0	41.0	30.0	41.0
36	36.29215678143761	39.0	35.0	40.0	30.0	41.0
37	36.25355347607134	39.0	35.0	40.0	30.0	41.0
38	36.14861247414083	39.0	35.0	40.0	29.0	41.0
39	36.15362995931644	39.0	35.0	40.0	29.0	41.0
40	36.02734506383587	39.0	35.0	40.0	29.0	41.0
41	35.90725485046604	39.0	35.0	40.0	28.0	41.0
42	35.80949912689307	39.0	35.0	40.0	27.0	41.0
43	35.62695527573131	38.0	35.0	40.0	27.0	41.0
44	35.45770154026198	38.0	34.0	40.0	26.0	41.0
45	35.29609152187835	38.0	34.0	40.0	26.0	41.0
46	35.1021811547127	38.0	34.0	40.0	24.0	41.0
47	34.974868803590105	38.0	34.0	40.0	24.0	41.0
48	34.89075105625204	38.0	34.0	40.0	23.0	41.0
49	34.699692684792275	38.0	34.0	40.0	23.0	41.0
50	34.53433222294405	38.0	33.0	40.0	23.0	41.0
51	32.41060675171972	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	7.0
9	19.0
10	14.0
11	12.0
12	7.0
13	8.0
14	9.0
15	16.0
16	28.0
17	53.0
18	130.0
19	249.0
20	509.0
21	830.0
22	1293.0
23	1916.0
24	2727.0
25	3638.0
26	4608.0
27	5586.0
28	6619.0
29	7800.0
30	9563.0
31	12150.0
32	15654.0
33	21462.0
34	30161.0
35	37325.0
36	43109.0
37	66316.0
38	82589.0
39	79657.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.920945812081584	22.472021415308628	28.78050690883289	10.82652586377689
2	29.679645781211843	25.389212176501214	28.210798881317356	16.720343160969588
3	31.067402011601498	23.837431637340412	28.503370330951295	16.5917960201068
4	27.32087485774577	26.463663547440348	28.13823194695933	18.077229647854555
5	20.95456618795527	40.043816606079034	22.46165471040034	16.539962495565355
6	23.792278878184305	35.70408356024898	25.973433590888355	14.530203970678352
7	71.62794126455371	18.22651019853392	5.967766458871826	4.177782078040555
8	87.43140696919015	3.3889910201298368	5.6512364023387285	3.528365608341281
9	82.2996576683668	5.706525495182937	7.36727162149087	4.6265452149593855
10	37.70900428951212	28.45867831423556	17.53862173506388	16.29369566118844
11	26.729051193092552	29.281333941513353	26.023654516888513	17.965960348505583
12	25.826226381190647	25.037665694500117	29.426237439009217	19.709870485300012
13	21.811547127040512	26.560880202358078	31.19917434954686	20.428398321054548
14	18.47692371487415	29.398132150146743	29.86233015881792	22.262613976161187
15	17.96526923484503	29.030459682732758	33.22206403398436	19.782207048437854
16	20.571919591229307	27.255449431213457	31.02178851000502	21.150842467552213
17	20.361360295980944	26.66915467584466	29.146566777705594	23.822918250468806
18	20.826479789532854	27.867315392022707	30.31063255329638	20.99557226514806
19	21.40263821121355	29.03506710713644	28.4522279200704	21.11006676157961
20	22.33886685004216	28.377357273510533	29.126294110329386	20.15748176611792
21	21.588778157122388	28.457756829354825	29.93789191903834	20.015573094484452
22	21.94608391962809	25.13925940260135	28.833261918255076	24.081394759515483
23	20.18789076718224	26.635059735257393	30.197520284185934	22.97952921337443
24	21.05362581263448	26.2805184273939	30.880570952032105	21.785284807939515
25	20.00290267737432	28.645048631364585	29.06524573698057	22.286802954280528
26	20.44613690500873	29.518385927082903	27.94541123566515	22.090065932243217
27	19.27585110647297	29.559622375495877	29.514930358780138	21.64959615925102
28	17.45799180799941	28.620398910804873	31.31850664160228	22.60310263959344
29	19.23622725660129	26.418971530724612	30.40969217797559	23.935109034698513
30	20.112559378182002	28.042397519362698	30.10007325804802	21.744969844407276
31	20.55464174971549	28.966416483521545	27.319031887984295	23.159909878778663
32	21.188392976442238	28.38104321303348	27.138881593800253	23.29168221672403
33	20.397528577549863	28.166567607041987	28.10874443077575	23.327159384632395
34	19.62762795969425	27.014941877341148	29.061559797457626	24.29587036550698
35	19.80294045825443	26.35746241493543	28.484479890896193	25.355117235913955
36	20.203556010154763	27.635561944517395	30.09408360632323	22.06679843900461
37	19.431121308877124	27.709971848636894	30.05238641546989	22.806520427016093
38	17.788574508963745	27.67910210513221	29.07399984334757	25.458323542556478
39	18.76074105814109	27.966835759142285	29.53220820029395	23.740214982422675
40	20.153565455374792	27.12091263862588	29.43291820439456	23.292603701604765
41	19.032348726738267	26.27660211665077	29.921996304845628	24.769052851765334
42	20.064411793163504	25.20675817011532	30.35279048659009	24.376039550131082
43	20.399832289751707	25.16989877488585	30.029118922231284	24.401150013131158
44	20.108412696218686	26.60418999175271	27.776088388829763	25.511308923198843
45	19.731986122437696	27.02093152906594	27.21237001303901	26.034712335457357
46	20.519855695467676	27.646159020645868	28.005538124133228	23.828447159753228
47	18.455268820176833	27.163761685580145	30.44263526246193	23.93833423178109
48	19.117816449426606	24.950815744490672	30.68383392999479	25.247533876087928
49	19.376753700913653	24.800613708930573	30.559203099875138	25.263429490280636
50	18.62320943969112	24.91602969024286	31.058647905234494	25.40211296483153
51	18.48429559392004	25.07982362779383	28.963191286438967	27.472689491847163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	116.0
1	347.0
2	578.0
3	938.0
4	1298.0
5	1018.0
6	738.0
7	624.5
8	511.0
9	561.0
10	611.0
11	679.5
12	748.0
13	784.0
14	820.0
15	847.0
16	874.0
17	851.5
18	829.0
19	858.0
20	887.0
21	1098.5
22	1310.0
23	1845.0
24	2380.0
25	3157.0
26	4409.0
27	4884.0
28	6004.5
29	7125.0
30	8035.5
31	8946.0
32	10443.5
33	11941.0
34	14280.0
35	16619.0
36	17940.5
37	19262.0
38	21117.5
39	22973.0
40	24668.5
41	26364.0
42	28569.5
43	30775.0
44	33436.5
45	36098.0
46	38323.5
47	40549.0
48	41433.0
49	42317.0
50	40333.0
51	38349.0
52	33694.0
53	29039.0
54	25375.0
55	21711.0
56	19423.5
57	17136.0
58	15265.5
59	13395.0
60	11724.5
61	10054.0
62	8783.5
63	7513.0
64	6454.5
65	5396.0
66	4343.0
67	3290.0
68	2511.0
69	1732.0
70	1422.0
71	1112.0
72	949.5
73	787.0
74	630.5
75	371.0
76	268.0
77	222.0
78	176.0
79	130.0
80	84.0
81	58.5
82	33.0
83	27.0
84	21.0
85	15.0
86	9.0
87	6.5
88	4.0
89	5.0
90	6.0
91	5.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.42303021085047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.97975255692893	29.559290502539014
2	9.039673985724228	7.127426812708906
3	3.8344100704253523	4.534921921415037
4	2.3371429950288563	3.6854903560040055
5	1.581551870688621	3.117478358909229
6	1.1783151483450494	2.787165221466578
7	0.8900841862227946	2.456287103456304
8	0.6641527476990318	2.0946331069726623
9	0.527712867425096	1.872363628363868
>10	4.874622369368396	39.82786157845474
>50	0.07802440935374182	1.9027329273218672
>100	0.013392249366687023	0.7972242075749598
>500	0.001164543423190176	0.23712427481282278
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	519	0.11956266327560232	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	514	0.11841080717468128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1290078833031547	0.0
2	0.0	0.0	0.0	0.5388382840108551	0.0
3	0.0	0.0	0.0	0.7634502236904548	0.0
4	0.0	0.0	0.0	1.060629097728079	0.0
5	0.0	0.0	0.0	1.8844365811067956	0.0
6	0.0	0.0	0.0	2.427421547080966	0.0
7	0.0	0.0	0.0	3.0127948175690307	0.0
8	0.0	0.0	0.0	4.082869135324662	0.0
9	0.0	0.0	0.0	4.475191323298363	0.0
10	0.0	0.0	0.0	5.445054160273865	0.0
11	0.0	0.0	0.0	6.738358190387991	0.0
12	0.0	0.0	0.0	7.696932837574468	0.0
13	0.0	0.0	0.0	8.090867624089459	0.0
14	0.0	0.0	0.0	8.235771121585323	0.0
15	0.0	0.0	0.0	8.528803313659632	0.0
16	0.0	0.0	0.0	9.255394142120613	0.0
17	0.0	0.0	0.0	10.14117148372888	0.0
18	0.0	0.0	0.0	11.140521836887961	0.0
19	0.0	0.0	0.0	11.715067660027367	0.0
20	0.0	0.0	0.0	12.245382208891408	0.0
21	0.0	0.0	0.0	12.945019604590838	0.0
22	0.0	0.0	0.0	13.708469828281293	0.0
23	0.0	0.0	0.0	14.456485180219406	0.0
24	0.0	0.0	0.0	15.028266548716601	0.0
25	0.0	0.0	0.0	15.544528453149406	0.0
26	0.0	0.0	0.0	16.01218203012334	0.0
27	0.0	0.0	0.0	16.462557765583462	0.0
28	0.0	0.0	0.0	16.943342502107896	0.0
29	0.0	0.0	0.0	17.468128141687515	0.0
30	0.0	0.0	0.0	18.038066540423237	0.0
31	0.0	0.0	0.0	18.601554544993803	0.0
32	0.0	0.0	0.0	19.09224524398616	0.0
33	0.0	0.0	0.0	19.6050515801162	0.0
34	0.0	0.0	0.0	20.13651798508116	0.0
35	0.0	0.0	0.0	20.66752364760575	0.0
36	0.0	0.0	0.0	21.17756552909358	0.0
37	0.0	0.0	0.0	21.661114720260226	0.0
38	0.0	0.0	0.0	22.171617344188427	0.0
39	0.0	0.0	0.0	22.71206822674057	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	45	3.8380676E-10	45.000004	28
GACGGTC	45	3.8380676E-10	45.000004	29
CGGTCTA	45	3.8380676E-10	45.000004	31
CTAGACG	20	7.0284307E-4	45.0	1
CGGGTCG	20	7.0284307E-4	45.0	6
GTACCGT	20	7.0284307E-4	45.0	25
TAGACCG	25	3.8867798E-5	45.0	1
GGCGTCA	35	1.2095916E-7	45.0	36
ATAGCGC	20	7.0284307E-4	45.0	12
TCGATGG	35	1.2095916E-7	45.0	2
TCGATCA	20	7.0284307E-4	45.0	17
TCGATAG	40	6.7975634E-9	45.0	1
CTCGGGT	20	7.0284307E-4	45.0	4
CTCGGCT	20	7.0284307E-4	45.0	36
AGTTGCG	20	7.0284307E-4	45.0	1
TGCGACG	20	7.0284307E-4	45.0	1
GCGATAT	20	7.0284307E-4	45.0	9
CCGACGG	20	7.0284307E-4	45.0	2
TATAGCG	20	7.0284307E-4	45.0	1
ACGTAGC	20	7.0284307E-4	45.0	30
>>END_MODULE